| Definition | Geobacillus thermodenitrificans NG80-2 chromosome, complete genome. |
|---|---|
| Accession | NC_009328 |
| Length | 3,550,319 |
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The map label for this gene is spoIIE [H]
Identifier: 138893736
GI number: 138893736
Start: 67440
End: 69917
Strand: Direct
Name: spoIIE [H]
Synonym: GTNG_0057
Alternate gene names: 138893736
Gene position: 67440-69917 (Clockwise)
Preceding gene: 138893735
Following gene: 138893737
Centisome position: 1.9
GC content: 48.1
Gene sequence:
>2478_bases ATGGAAAGAGTAGAAAGAGGAACGGTGCGTCGCATTTCAGAAGTACGGATTAATGAAACGCAAGCAACCTTGTCGCGCTG GATGCGCCATGTGAAGTTGCGACTTGGCCATCTGTTCGTTCATCAAGGCTTTTTGCTTCTCCTTGTCGGCTTTTTGTTGG GACGGGCGTTAATTTTGGCGAAATTGACCCCGTTTGCGCTGCCGTTTTTTGTCTCTGTCTACATGTTGCGTCGTGACAAG GCAGCTTTCGCTTTAGTTTCACTGCTGGCCGGCGCTGTGACGCTGTCGTTTGATACGGTGTTGTTTGTCGGTGCTTCCAT TTTTGGTTTCCTCTTTATTTATAGCTGGGTGCGGAAAGTGATTAGTGAATCATTAAAACTTGTGCCGTTCGTCGTTTTCG CTGTATCGTTGGCGACAAAATGGCTCATTTCTTATTACTGGCTTGGGGAACGGACGGCATACGGAGTGGCTATGGCGGTT GTAGAAGCCGGGTTGTCACTCGTGCTGACATTGATTTTTATGCAAAGCATTCCGCTGTTGACGGTACAGAAACATAAACA TGCGCTGCGGGCGGAAGAAATGATTTCATTGATTATTTTGCTGGCGTCGATGCTGACTGGGATGATCGGCTGGTCGGCGT ACGGCTTATCGCTTGAACATGTCATGTCGCGCTATCTCGTTTTATTATTTGCATTTGTAGCTGGGGCAGCGACTGGTTCG ACCGTCGGGGTCGTCACTGGGCTCGTTCTGAGCTTAGCGAACGTCGGTAACTTGTCTGAAATGAGCTTGCTTGCTTTTGC CGGCCTGCTTGGCGGCCTGCTGAAAGAAGGAAAAAAGATTGGGGTCGCGTTCGGACTCATTGTCGCAACGCTGTTGATGG GCTTATACGGGGAAGGGAAAGCCGAACTCATGCCGACGCTGCTTGAATCAAGCATCGCTATCATGCTGTTTTTATGTACA GCCCATTCGCTGACAGAGAAGATTGCCAAACATATTCCTGGCACGGCTGAATATATCAACGAACAACAGCAATACGTTCG CAAAATCCGTGATGTAACGGCACAGCGCGTTTCGCAGTTTTCGAACGTCTTTCAAGCGCTGGCGGAAAGCTTTTCGCCTG GACCGCTGTCGGATTCTGATGAATACCATGAACGGGAGGTTGATTATTTTCTTGGTGATATTACAGAAAAGACGTGCCAA ACGTGCTTTAAGAAAGCGCAATGCTGGGTAGCCAACTTTAGTACAACATATGCGTATATGAAACAAATGATGCATGAGGC GGACGAAAAAACGTTGGCACAAAACCATCAGTTGTTACGCGAATGGGAGCGGTATTGCGTCAAAGCAGGCAAAGTGGTTG ACACGATTGAAAAAGAGATGGTGATTTATCAGGCGAACCGTAAGCTCAAACGGCAAGTTTACGAAAGCCGGAAGCTTGTT GCCGAGCAATTGTTGGGTGTTTCGCAAGTCATGGACGATTTTGCGAAGGAGATTCAAAAAGAGCGAGAAAACCATTATTT GCAAGAGGAACAAATCTACCATGCCCTGCAAGAGTTTGGCATCGAAATCGGGCATGTCGATATTTACAGTTTAGAAAAAG GAAATATCGATATTGAAATGAGCATTCCTTACTGCGAGGGGCGTGGTGAGTGCGAAAAACTGATCGCGCCGATGCTGTCG GACATTTTAGGGGAGACGATTGTCGTCAAGCGCGAGGAATGTGCTGCCTATCCAAATGGCTATTGCCGGGTTGCCTTCGG TTCGACAAAAGCGTTTGTCGTCGAGACAGGCGTGGCGTTTGCTGCTAAAGGAGGCGGGCTCATTTCTGGTGACAGCTATG CAACGATTGAACTTGGCACCGGTAAATACGCTGTCGCCATTAGCGACGGCATGGGCAACGGGGAACGAGCGCATTACGAG AGCCACGAGACGTTACATTTGCTGCAAAAAATCTTGCAGACAGGAATGGATGAATCGATCGCCATTAAATCGATCAATTC CATTTTGGCGCTGCGGACGACAGAAGATATGTACGCGACGCTTGATTTGGCGATCATCGATTTGCAAAACGCGGCGACGA AGTTTTTGAAAATCGGGTCGATGCCAAGCTTTATTAAACGCGGCGATAAAGTGATGAAAGTAGAGGCGAGCAATTTGCCA ATGGGGATTATTAAAGAAGTCGAATTCGATGTGGTCAGTGAACAGCTGAAATCCGGCGACTTGCTCATTATGATGAGCGA CGGTGTGTTTGAAGGGCCAATGAATGTGGAGAACCATGATTTGTGGATGAAACGGAAAATCCAAGAACTGAAAACGACCG ATCCACAAGAAGTCGCCGATGTGATCATGGAGGAAGTGATCCGGCAGTGCGGCGGAGAGATTGAAGATGATATGACCGTT GTTGTCGCTAAAGTGCGGCACAACACGCCAAAATGGGCGACGATTCCGGCATATATGTATATGAAAAAGGCGCAGTAG
Upstream 100 bases:
>100_bases ACGTCGAACGATTTTTTATTTTGCTCCGTTGTTTTGACAAATTTCAGATAATTTTAACTATATAATAGGGGGTAATAGAA AGCAAAGGAGAGGATCGGGA
Downstream 100 bases:
>100_bases TACGCGTATAAAAACGTTCTCCCGCGGCGATGATGGTAATACATTCCGCTGAGGAGGGGAACGAAGATGCGAAAGGGAAC GTTGCGGCAGATTTTGCTCA
Product: Serine phosphatase
Products: NA
Alternate protein names: Stage II sporulation protein H [H]
Number of amino acids: Translated: 825; Mature: 825
Protein sequence:
>825_residues MERVERGTVRRISEVRINETQATLSRWMRHVKLRLGHLFVHQGFLLLLVGFLLGRALILAKLTPFALPFFVSVYMLRRDK AAFALVSLLAGAVTLSFDTVLFVGASIFGFLFIYSWVRKVISESLKLVPFVVFAVSLATKWLISYYWLGERTAYGVAMAV VEAGLSLVLTLIFMQSIPLLTVQKHKHALRAEEMISLIILLASMLTGMIGWSAYGLSLEHVMSRYLVLLFAFVAGAATGS TVGVVTGLVLSLANVGNLSEMSLLAFAGLLGGLLKEGKKIGVAFGLIVATLLMGLYGEGKAELMPTLLESSIAIMLFLCT AHSLTEKIAKHIPGTAEYINEQQQYVRKIRDVTAQRVSQFSNVFQALAESFSPGPLSDSDEYHEREVDYFLGDITEKTCQ TCFKKAQCWVANFSTTYAYMKQMMHEADEKTLAQNHQLLREWERYCVKAGKVVDTIEKEMVIYQANRKLKRQVYESRKLV AEQLLGVSQVMDDFAKEIQKERENHYLQEEQIYHALQEFGIEIGHVDIYSLEKGNIDIEMSIPYCEGRGECEKLIAPMLS DILGETIVVKREECAAYPNGYCRVAFGSTKAFVVETGVAFAAKGGGLISGDSYATIELGTGKYAVAISDGMGNGERAHYE SHETLHLLQKILQTGMDESIAIKSINSILALRTTEDMYATLDLAIIDLQNAATKFLKIGSMPSFIKRGDKVMKVEASNLP MGIIKEVEFDVVSEQLKSGDLLIMMSDGVFEGPMNVENHDLWMKRKIQELKTTDPQEVADVIMEEVIRQCGGEIEDDMTV VVAKVRHNTPKWATIPAYMYMKKAQ
Sequences:
>Translated_825_residues MERVERGTVRRISEVRINETQATLSRWMRHVKLRLGHLFVHQGFLLLLVGFLLGRALILAKLTPFALPFFVSVYMLRRDK AAFALVSLLAGAVTLSFDTVLFVGASIFGFLFIYSWVRKVISESLKLVPFVVFAVSLATKWLISYYWLGERTAYGVAMAV VEAGLSLVLTLIFMQSIPLLTVQKHKHALRAEEMISLIILLASMLTGMIGWSAYGLSLEHVMSRYLVLLFAFVAGAATGS TVGVVTGLVLSLANVGNLSEMSLLAFAGLLGGLLKEGKKIGVAFGLIVATLLMGLYGEGKAELMPTLLESSIAIMLFLCT AHSLTEKIAKHIPGTAEYINEQQQYVRKIRDVTAQRVSQFSNVFQALAESFSPGPLSDSDEYHEREVDYFLGDITEKTCQ TCFKKAQCWVANFSTTYAYMKQMMHEADEKTLAQNHQLLREWERYCVKAGKVVDTIEKEMVIYQANRKLKRQVYESRKLV AEQLLGVSQVMDDFAKEIQKERENHYLQEEQIYHALQEFGIEIGHVDIYSLEKGNIDIEMSIPYCEGRGECEKLIAPMLS DILGETIVVKREECAAYPNGYCRVAFGSTKAFVVETGVAFAAKGGGLISGDSYATIELGTGKYAVAISDGMGNGERAHYE SHETLHLLQKILQTGMDESIAIKSINSILALRTTEDMYATLDLAIIDLQNAATKFLKIGSMPSFIKRGDKVMKVEASNLP MGIIKEVEFDVVSEQLKSGDLLIMMSDGVFEGPMNVENHDLWMKRKIQELKTTDPQEVADVIMEEVIRQCGGEIEDDMTV VVAKVRHNTPKWATIPAYMYMKKAQ >Mature_825_residues MERVERGTVRRISEVRINETQATLSRWMRHVKLRLGHLFVHQGFLLLLVGFLLGRALILAKLTPFALPFFVSVYMLRRDK AAFALVSLLAGAVTLSFDTVLFVGASIFGFLFIYSWVRKVISESLKLVPFVVFAVSLATKWLISYYWLGERTAYGVAMAV VEAGLSLVLTLIFMQSIPLLTVQKHKHALRAEEMISLIILLASMLTGMIGWSAYGLSLEHVMSRYLVLLFAFVAGAATGS TVGVVTGLVLSLANVGNLSEMSLLAFAGLLGGLLKEGKKIGVAFGLIVATLLMGLYGEGKAELMPTLLESSIAIMLFLCT AHSLTEKIAKHIPGTAEYINEQQQYVRKIRDVTAQRVSQFSNVFQALAESFSPGPLSDSDEYHEREVDYFLGDITEKTCQ TCFKKAQCWVANFSTTYAYMKQMMHEADEKTLAQNHQLLREWERYCVKAGKVVDTIEKEMVIYQANRKLKRQVYESRKLV AEQLLGVSQVMDDFAKEIQKERENHYLQEEQIYHALQEFGIEIGHVDIYSLEKGNIDIEMSIPYCEGRGECEKLIAPMLS DILGETIVVKREECAAYPNGYCRVAFGSTKAFVVETGVAFAAKGGGLISGDSYATIELGTGKYAVAISDGMGNGERAHYE SHETLHLLQKILQTGMDESIAIKSINSILALRTTEDMYATLDLAIIDLQNAATKFLKIGSMPSFIKRGDKVMKVEASNLP MGIIKEVEFDVVSEQLKSGDLLIMMSDGVFEGPMNVENHDLWMKRKIQELKTTDPQEVADVIMEEVIRQCGGEIEDDMTV VVAKVRHNTPKWATIPAYMYMKKAQ
Specific function: Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates spoIIAA by dephosphorylation [H]
COG id: COG2208
COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein. Note=Polar septum [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PP2C-like domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001932 - InterPro: IPR014221 - InterPro: IPR010822 [H]
Pfam domain/function: PF07228 SpoIIE [H]
EC number: =3.1.3.16 [H]
Molecular weight: Translated: 91988; Mature: 91988
Theoretical pI: Translated: 6.48; Mature: 6.48
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 4.1 %Met (Translated Protein) 5.3 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 4.1 %Met (Mature Protein) 5.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MERVERGTVRRISEVRINETQATLSRWMRHVKLRLGHLFVHQGFLLLLVGFLLGRALILA CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KLTPFALPFFVSVYMLRRDKAAFALVSLLAGAVTLSFDTVLFVGASIFGFLFIYSWVRKV HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ISESLKLVPFVVFAVSLATKWLISYYWLGERTAYGVAMAVVEAGLSLVLTLIFMQSIPLL HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH TVQKHKHALRAEEMISLIILLASMLTGMIGWSAYGLSLEHVMSRYLVLLFAFVAGAATGS HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC TVGVVTGLVLSLANVGNLSEMSLLAFAGLLGGLLKEGKKIGVAFGLIVATLLMGLYGEGK HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCH AELMPTLLESSIAIMLFLCTAHSLTEKIAKHIPGTAEYINEQQQYVRKIRDVTAQRVSQF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH SNVFQALAESFSPGPLSDSDEYHEREVDYFLGDITEKTCQTCFKKAQCWVANFSTTYAYM HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KQMMHEADEKTLAQNHQLLREWERYCVKAGKVVDTIEKEMVIYQANRKLKRQVYESRKLV HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH AEQLLGVSQVMDDFAKEIQKERENHYLQEEQIYHALQEFGIEIGHVDIYSLEKGNIDIEM HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEECCEEEEEECCCCEEEEE SIPYCEGRGECEKLIAPMLSDILGETIVVKREECAAYPNGYCRVAFGSTKAFVVETGVAF ECCCCCCCCHHHHHHHHHHHHHCCCCEEEECHHHCCCCCCEEEEEECCCEEEEEECCCCE AAKGGGLISGDSYATIELGTGKYAVAISDGMGNGERAHYESHETLHLLQKILQTGMDESI EECCCCEECCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHH AIKSINSILALRTTEDMYATLDLAIIDLQNAATKFLKIGSMPSFIKRGDKVMKVEASNLP HHHHHHHHHHEEECHHHHHHHHHHEEEHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCCC MGIIKEVEFDVVSEQLKSGDLLIMMSDGVFEGPMNVENHDLWMKRKIQELKTTDPQEVAD HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH VIMEEVIRQCGGEIEDDMTVVVAKVRHNTPKWATIPAYMYMKKAQ HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEECHHHHHHCCCC >Mature Secondary Structure MERVERGTVRRISEVRINETQATLSRWMRHVKLRLGHLFVHQGFLLLLVGFLLGRALILA CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KLTPFALPFFVSVYMLRRDKAAFALVSLLAGAVTLSFDTVLFVGASIFGFLFIYSWVRKV HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ISESLKLVPFVVFAVSLATKWLISYYWLGERTAYGVAMAVVEAGLSLVLTLIFMQSIPLL HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH TVQKHKHALRAEEMISLIILLASMLTGMIGWSAYGLSLEHVMSRYLVLLFAFVAGAATGS HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC TVGVVTGLVLSLANVGNLSEMSLLAFAGLLGGLLKEGKKIGVAFGLIVATLLMGLYGEGK HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCH AELMPTLLESSIAIMLFLCTAHSLTEKIAKHIPGTAEYINEQQQYVRKIRDVTAQRVSQF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH SNVFQALAESFSPGPLSDSDEYHEREVDYFLGDITEKTCQTCFKKAQCWVANFSTTYAYM HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KQMMHEADEKTLAQNHQLLREWERYCVKAGKVVDTIEKEMVIYQANRKLKRQVYESRKLV HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH AEQLLGVSQVMDDFAKEIQKERENHYLQEEQIYHALQEFGIEIGHVDIYSLEKGNIDIEM HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEECCEEEEEECCCCEEEEE SIPYCEGRGECEKLIAPMLSDILGETIVVKREECAAYPNGYCRVAFGSTKAFVVETGVAF ECCCCCCCCHHHHHHHHHHHHHCCCCEEEECHHHCCCCCCEEEEEECCCEEEEEECCCCE AAKGGGLISGDSYATIELGTGKYAVAISDGMGNGERAHYESHETLHLLQKILQTGMDESI EECCCCEECCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHH AIKSINSILALRTTEDMYATLDLAIIDLQNAATKFLKIGSMPSFIKRGDKVMKVEASNLP HHHHHHHHHHEEECHHHHHHHHHHEEEHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCCC MGIIKEVEFDVVSEQLKSGDLLIMMSDGVFEGPMNVENHDLWMKRKIQELKTTDPQEVAD HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH VIMEEVIRQCGGEIEDDMTVVVAKVRHNTPKWATIPAYMYMKKAQ HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEECHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 7584024; 8830262; 9384377; 2832371; 8113187; 7570023 [H]