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The map label for this gene is recF [H]

Identifier: 138893683

GI number: 138893683

Start: 3292

End: 4410

Strand: Direct

Name: recF [H]

Synonym: GTNG_0004

Alternate gene names: 138893683

Gene position: 3292-4410 (Clockwise)

Preceding gene: 138893682

Following gene: 138893684

Centisome position: 0.09

GC content: 47.36

Gene sequence:

>1119_bases
GTGTTTCTGACTAACTTAACACTAACGAACTACCGCAATTATGAACATGAGACGTTAAGTTTTGACCAAGGCGTTAACAT
CATCTTAGGGGAAAACGCCCAAGGAAAGACGAACATGATGGAGGCCATTTATGTTCTGGCCATGGCAAAATCGCACCGCA
CCACGAACGATAAAGACCTTATCCGTTGGAATGAAGACTATGCTAAAATAGAAGGAAGAGCAGAAAAGCGAAGCGGTTCT
TTAGCGCTTGAACTGACGATCTCAAAAAAGGGAAAAAAGGCAAGATGTAACCATATTGAACAGCAGCGGCTCAGCCAATA
TGTTGGCCATTTAAACGTTGTCATGTTCGCTCCTGAGGACTTGAATTTAGTCAAAGGAAGCCCGCAAGTACGGCGCCGGT
TTATCGACATGGAAATCGGGCAAGTATCGCCCGTTTATATACATGATTTAAGCCAATATCAAAAGCTGTTGCAGCAGCGC
AATCATTACTTGAAAATGATGCAGGCACGTGAGCAACACGATGAAGCAGTGCTCGACGTATTAACCGAGCAGCTCATGGT
GCTGGCGGCAAAAATCACCCTGCGGCGCCGCCAGTTTTTAGCGTTGCTCGAGCAATGGGCAATGCCGATCCACCATGAAA
TCAGTCGTGGCGCCGAACGGCTTCACATTCGATACGAACCATCAGTTGACGTATCAGAAAAGGCAGAATTGTCGAGAATA
GTAGAAGCATACAGTGAAACGTTTGCCGCCATGAGAGAGCGGGAAATTCAGCGGGGAACGACGCTTGTCGGCCCACACCG
TGATGACATTGCGTTTATCGTCAATGGAAAAAACGTGCAAACATTCGGCTCCCAAGGGCAGCAGCGCACAACAGCGCTGG
CGGTCAAACTAGCGGAAATTGAACTCATCTTTTCTGAGTTAGGAGATTACCCCATTTTATTGCTCGATGATGTGTTGTCG
GAACTGGATGACTTCCGACAAACCCACCTCCTTGATGCGATCCGGAAAAAAGTGCAAACTTTTGTCACTACAACAAGTAT
CGACGGCATTAAGCACGATCTCATTCAAGAAGCGGCCATTTATCGAGTTCACTCCGGTTCGGTAGCCGCCCCTTCTTGA

Upstream 100 bases:

>100_bases
AAGAGGACCGGCGCGGCCGGAAACTGAAAGACGGCGACCGAGTTGAGATTGAACGGATCGGCACGTTTATCGTCACGCAA
GTGGAGTAGGTGAAGGCCGG

Downstream 100 bases:

>100_bases
TTGTGGAACGTTGAACGTTTGCAAAGAAAGCGTAGGTGATATGGCATGACAATGGAACAACGGGTTGAGCAGGCGTACGA
TGCGAGCCAAATCCAAGTGC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MFLTNLTLTNYRNYEHETLSFDQGVNIILGENAQGKTNMMEAIYVLAMAKSHRTTNDKDLIRWNEDYAKIEGRAEKRSGS
LALELTISKKGKKARCNHIEQQRLSQYVGHLNVVMFAPEDLNLVKGSPQVRRRFIDMEIGQVSPVYIHDLSQYQKLLQQR
NHYLKMMQAREQHDEAVLDVLTEQLMVLAAKITLRRRQFLALLEQWAMPIHHEISRGAERLHIRYEPSVDVSEKAELSRI
VEAYSETFAAMREREIQRGTTLVGPHRDDIAFIVNGKNVQTFGSQGQQRTTALAVKLAEIELIFSELGDYPILLLDDVLS
ELDDFRQTHLLDAIRKKVQTFVTTTSIDGIKHDLIQEAAIYRVHSGSVAAPS

Sequences:

>Translated_372_residues
MFLTNLTLTNYRNYEHETLSFDQGVNIILGENAQGKTNMMEAIYVLAMAKSHRTTNDKDLIRWNEDYAKIEGRAEKRSGS
LALELTISKKGKKARCNHIEQQRLSQYVGHLNVVMFAPEDLNLVKGSPQVRRRFIDMEIGQVSPVYIHDLSQYQKLLQQR
NHYLKMMQAREQHDEAVLDVLTEQLMVLAAKITLRRRQFLALLEQWAMPIHHEISRGAERLHIRYEPSVDVSEKAELSRI
VEAYSETFAAMREREIQRGTTLVGPHRDDIAFIVNGKNVQTFGSQGQQRTTALAVKLAEIELIFSELGDYPILLLDDVLS
ELDDFRQTHLLDAIRKKVQTFVTTTSIDGIKHDLIQEAAIYRVHSGSVAAPS
>Mature_372_residues
MFLTNLTLTNYRNYEHETLSFDQGVNIILGENAQGKTNMMEAIYVLAMAKSHRTTNDKDLIRWNEDYAKIEGRAEKRSGS
LALELTISKKGKKARCNHIEQQRLSQYVGHLNVVMFAPEDLNLVKGSPQVRRRFIDMEIGQVSPVYIHDLSQYQKLLQQR
NHYLKMMQAREQHDEAVLDVLTEQLMVLAAKITLRRRQFLALLEQWAMPIHHEISRGAERLHIRYEPSVDVSEKAELSRI
VEAYSETFAAMREREIQRGTTLVGPHRDDIAFIVNGKNVQTFGSQGQQRTTALAVKLAEIELIFSELGDYPILLLDDVLS
ELDDFRQTHLLDAIRKKVQTFVTTTSIDGIKHDLIQEAAIYRVHSGSVAAPS

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=370, Percent_Identity=26.7567567567568, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 42583; Mature: 42583

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLTNLTLTNYRNYEHETLSFDQGVNIILGENAQGKTNMMEAIYVLAMAKSHRTTNDKDL
CEEEEEEEECCCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
IRWNEDYAKIEGRAEKRSGSLALELTISKKGKKARCNHIEQQRLSQYVGHLNVVMFAPED
EEECCHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCC
LNLVKGSPQVRRRFIDMEIGQVSPVYIHDLSQYQKLLQQRNHYLKMMQAREQHDEAVLDV
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTEQLMVLAAKITLRRRQFLALLEQWAMPIHHEISRGAERLHIRYEPSVDVSEKAELSRI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHEEEEEECCCCCCHHHHHHHHH
VEAYSETFAAMREREIQRGTTLVGPHRDDIAFIVNGKNVQTFGSQGQQRTTALAVKLAEI
HHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCEEEECCCCCCHHHHHHHHHHHHH
ELIFSELGDYPILLLDDVLSELDDFRQTHLLDAIRKKVQTFVTTTSIDGIKHDLIQEAAI
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YRVHSGSVAAPS
EEEECCCCCCCC
>Mature Secondary Structure
MFLTNLTLTNYRNYEHETLSFDQGVNIILGENAQGKTNMMEAIYVLAMAKSHRTTNDKDL
CEEEEEEEECCCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
IRWNEDYAKIEGRAEKRSGSLALELTISKKGKKARCNHIEQQRLSQYVGHLNVVMFAPED
EEECCHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCC
LNLVKGSPQVRRRFIDMEIGQVSPVYIHDLSQYQKLLQQRNHYLKMMQAREQHDEAVLDV
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTEQLMVLAAKITLRRRQFLALLEQWAMPIHHEISRGAERLHIRYEPSVDVSEKAELSRI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHEEEEEECCCCCCHHHHHHHHH
VEAYSETFAAMREREIQRGTTLVGPHRDDIAFIVNGKNVQTFGSQGQQRTTALAVKLAEI
HHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCEEEECCCCCCHHHHHHHHHHHHH
ELIFSELGDYPILLLDDVLSELDDFRQTHLLDAIRKKVQTFVTTTSIDGIKHDLIQEAAI
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YRVHSGSVAAPS
EEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA