Click here to switch to the map view.

The map label for this gene is gyrB [H]

Identifier: 138893684

GI number: 138893684

Start: 4456

End: 6378

Strand: Direct

Name: gyrB [H]

Synonym: GTNG_0005

Alternate gene names: 138893684

Gene position: 4456-6378 (Clockwise)

Preceding gene: 138893683

Following gene: 138893685

Centisome position: 0.13

GC content: 50.86

Gene sequence:

>1923_bases
ATGACAATGGAACAACGGGTTGAGCAGGCGTACGATGCGAGCCAAATCCAAGTGCTTGAAGGCCTTGAGGCGGTTCGGAA
ACGCCCAGGAATGTACATCGGTTCGACAGGACCAAAAGGGCTTCACCATCTCGTCTGGGAAATCGTCGACAACAGTATCG
ATGAGGCATTGGCCGGCTATTGCACGGAAATTCATGTGACGATCGGAGAAGATAACAGCATTACGATCGCTGACAACGGG
CGCGGTATTCCGGTTGATGTGCACGAGGCGACGGGGCGTCCAGCTGTTGAGGTCATTATGACTGTCCTTCACGCCGGCGG
AAAGTTTGGTGGAGGGGGCTACAAAGTATCGGGCGGCTTGCATGGCGTCGGTGCCTCAGTTGTGAACGCCTTATCGGAAT
GGCTCGAAGTTTACGTGTATCGTGACGGAAAAATCCACTACCAAAAATACGAACGAGGTGAACCTCGCACCGACCTGCAA
GTTATCGACGAAACGGACCGAACCGGGACGACGACCCGTTTCAAGCCGGACCCGGAAATTTTTACAGAAACAACCGAGTT
TGACTATGAGACGCTGGCTACCCGGCTGCGCGAGCTCGCTTTTTTAAACCGCGGCTTGAAAATTACCCTAACGGACGAAC
GGGTTGACAACCGGAAAAATGAATATTTTTATGAGGGTGGTATTCGCTCTTATGTTCGCCATTTAAACCGGACACGGGAA
GTGCTGCACGAAGAGCCGATTTACATTGCTGGCGAGCGCGACGGCATCGCCGTTGAAATTGCCTTGCAATATAACGACGG
CTACACAAGCAACATTTACTCATTCGTTAACAACATCCATACGCATGAAGGGGGCACGCATGAGTCCGGCTTTAAAATGG
CGCTGACGCGCATTATCAATGACTATGCTCGCAAACAGCAAATTTTTAAAGACAACGATGCCAACTTGACCGGTGAGGAT
GTGCGCGAGGGACTGACAGCCATCGTGTCGATCAAGCACCCGGCTCCGCAGTTTGAGGGGCAGACGAAAACAAAACTTGG
CAACAGCGATGCGCGCACTGTGACAGACGCCGTTTTCTCTGAGCAGTTCGAGACGTTTTTACTTGAACATCCAGCTGTTG
CTCGAAAAATTGTCGAAAAAGGTATGATGGCAGCGCGCGCCCGGGTCGCGGCGAAAAAAGCGCGCGAACTGACACGTCGG
AAAAGCGCGCTTGAAATTTCCAACTTGCCCGGCAAGCTGGCTGATTGTTCATCGAGAGATCCATCAATCAGCGAACTGTA
CGTCGTAGAGGGCGATTCGGCGGGTGGTTCAGCAAAACAAGGGCGCGACCGACATTTCCAAGCGATTTTACCGCTGCGCG
GGAAAATTCTTAACGTAGAAAAAGCGCGTTTGGACAAGATTTTGTCGAATAATGAGGTGCGGGCAATCATCACTGCGCTT
GGCACCGGCATTGGTGAAGACTTTGACATCTCAAAAGCGCGTTACCATAAAGTCATTATCATGACCGACGCGGACGTCGA
CGGCGCCCATATCCGCACGCTCCTTTTGACGTTCTTTTACCGCTATATGCGTGAATTGATCGAACGCGGCTACATTTATA
TCGCCCAGCCGCCGCTATATAAAATCGAGCAAGGAAAACAAGTGCGTTATGCTTACAATGACCGTCAGCTCGAAAAAATT
CTTGCCGAACTGCCGGCTCAGCCAAAACCGATGATTCAGCGCTACAAAGGATTGGGCGAGATGAATCCAGAGCAACTTTG
GGAAACGACGATGAACCCGGAAACGAGAACATTGCTGCAAGTGAGCCTACAAGACGCGATTGACGCGGATGAAACATTTG
AAATTTTAATGGGCGACAAGGTGGAACCGCGTCGTCAGTTTATTGAAGAAAACGCCCGTTATGTGAAAAACTTGGATATT
TAG

Upstream 100 bases:

>100_bases
TCAAGAAGCGGCCATTTATCGAGTTCACTCCGGTTCGGTAGCCGCCCCTTCTTGATTGTGGAACGTTGAACGTTTGCAAA
GAAAGCGTAGGTGATATGGC

Downstream 100 bases:

>100_bases
GCTGAGAAGAGAGGGAAGCGGCCGCCCTCCCCGCTTCACCTTGCCGACGGGATCACCCGGCGCGCGAGCGAAGCGGCGCG
CGCCGCTTGTCTGTTTCTGC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MTMEQRVEQAYDASQIQVLEGLEAVRKRPGMYIGSTGPKGLHHLVWEIVDNSIDEALAGYCTEIHVTIGEDNSITIADNG
RGIPVDVHEATGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVYVYRDGKIHYQKYERGEPRTDLQ
VIDETDRTGTTTRFKPDPEIFTETTEFDYETLATRLRELAFLNRGLKITLTDERVDNRKNEYFYEGGIRSYVRHLNRTRE
VLHEEPIYIAGERDGIAVEIALQYNDGYTSNIYSFVNNIHTHEGGTHESGFKMALTRIINDYARKQQIFKDNDANLTGED
VREGLTAIVSIKHPAPQFEGQTKTKLGNSDARTVTDAVFSEQFETFLLEHPAVARKIVEKGMMAARARVAAKKARELTRR
KSALEISNLPGKLADCSSRDPSISELYVVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRAIITAL
GTGIGEDFDISKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRELIERGYIYIAQPPLYKIEQGKQVRYAYNDRQLEKI
LAELPAQPKPMIQRYKGLGEMNPEQLWETTMNPETRTLLQVSLQDAIDADETFEILMGDKVEPRRQFIEENARYVKNLDI

Sequences:

>Translated_640_residues
MTMEQRVEQAYDASQIQVLEGLEAVRKRPGMYIGSTGPKGLHHLVWEIVDNSIDEALAGYCTEIHVTIGEDNSITIADNG
RGIPVDVHEATGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVYVYRDGKIHYQKYERGEPRTDLQ
VIDETDRTGTTTRFKPDPEIFTETTEFDYETLATRLRELAFLNRGLKITLTDERVDNRKNEYFYEGGIRSYVRHLNRTRE
VLHEEPIYIAGERDGIAVEIALQYNDGYTSNIYSFVNNIHTHEGGTHESGFKMALTRIINDYARKQQIFKDNDANLTGED
VREGLTAIVSIKHPAPQFEGQTKTKLGNSDARTVTDAVFSEQFETFLLEHPAVARKIVEKGMMAARARVAAKKARELTRR
KSALEISNLPGKLADCSSRDPSISELYVVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRAIITAL
GTGIGEDFDISKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRELIERGYIYIAQPPLYKIEQGKQVRYAYNDRQLEKI
LAELPAQPKPMIQRYKGLGEMNPEQLWETTMNPETRTLLQVSLQDAIDADETFEILMGDKVEPRRQFIEENARYVKNLDI
>Mature_639_residues
TMEQRVEQAYDASQIQVLEGLEAVRKRPGMYIGSTGPKGLHHLVWEIVDNSIDEALAGYCTEIHVTIGEDNSITIADNGR
GIPVDVHEATGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVYVYRDGKIHYQKYERGEPRTDLQV
IDETDRTGTTTRFKPDPEIFTETTEFDYETLATRLRELAFLNRGLKITLTDERVDNRKNEYFYEGGIRSYVRHLNRTREV
LHEEPIYIAGERDGIAVEIALQYNDGYTSNIYSFVNNIHTHEGGTHESGFKMALTRIINDYARKQQIFKDNDANLTGEDV
REGLTAIVSIKHPAPQFEGQTKTKLGNSDARTVTDAVFSEQFETFLLEHPAVARKIVEKGMMAARARVAAKKARELTRRK
SALEISNLPGKLADCSSRDPSISELYVVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRAIITALG
TGIGEDFDISKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRELIERGYIYIAQPPLYKIEQGKQVRYAYNDRQLEKIL
AELPAQPKPMIQRYKGLGEMNPEQLWETTMNPETRTLLQVSLQDAIDADETFEILMGDKVEPRRQFIEENARYVKNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=615, Percent_Identity=27.3170731707317, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI19913406, Length=606, Percent_Identity=25.9075907590759, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI48994957, Length=555, Percent_Identity=58.1981981981982, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=629, Percent_Identity=41.335453100159, Blast_Score=462, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17535065, Length=563, Percent_Identity=26.8206039076377, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI212645845, Length=594, Percent_Identity=24.2424242424242, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=209, Percent_Identity=28.2296650717703, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=624, Percent_Identity=26.2820512820513, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI17136538, Length=617, Percent_Identity=25.1215559157212, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72223; Mature: 72091

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMEQRVEQAYDASQIQVLEGLEAVRKRPGMYIGSTGPKGLHHLVWEIVDNSIDEALAGY
CCHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
CTEIHVTIGEDNSITIADNGRGIPVDVHEATGRPAVEVIMTVLHAGGKFGGGGYKVSGGL
EEEEEEEECCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGASVVNALSEWLEVYVYRDGKIHYQKYERGEPRTDLQVIDETDRTGTTTRFKPDPEI
CCHHHHHHHHHHHHHHEEEEECCEEEEEHHCCCCCCCCCEEEECCCCCCCCCCCCCCHHH
FTETTEFDYETLATRLRELAFLNRGLKITLTDERVDNRKNEYFYEGGIRSYVRHLNRTRE
HCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHCCCCCCEEECCCHHHHHHHHHHHHH
VLHEEPIYIAGERDGIAVEIALQYNDGYTSNIYSFVNNIHTHEGGTHESGFKMALTRIIN
HHHCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
DYARKQQIFKDNDANLTGEDVREGLTAIVSIKHPAPQFEGQTKTKLGNSDARTVTDAVFS
HHHHHHHHHCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCHHCCCCCHHHHHHHHHHH
EQFETFLLEHPAVARKIVEKGMMAARARVAAKKARELTRRKSALEISNLPGKLADCSSRD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PSISELYVVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRAIITAL
CCCEEEEEEECCCCCCCCCCCCCCHHEEEECCCCEEECHHHHHHHHHHCCCCHHHHHHHH
GTGIGEDFDISKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRELIERGYIYIAQPPLY
HCCCCCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCE
KIEQGKQVRYAYNDRQLEKILAELPAQPKPMIQRYKGLGEMNPEQLWETTMNPETRTLLQ
ECCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHH
VSLQDAIDADETFEILMGDKVEPRRQFIEENARYVKNLDI
HHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TMEQRVEQAYDASQIQVLEGLEAVRKRPGMYIGSTGPKGLHHLVWEIVDNSIDEALAGY
CHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
CTEIHVTIGEDNSITIADNGRGIPVDVHEATGRPAVEVIMTVLHAGGKFGGGGYKVSGGL
EEEEEEEECCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGASVVNALSEWLEVYVYRDGKIHYQKYERGEPRTDLQVIDETDRTGTTTRFKPDPEI
CCHHHHHHHHHHHHHHEEEEECCEEEEEHHCCCCCCCCCEEEECCCCCCCCCCCCCCHHH
FTETTEFDYETLATRLRELAFLNRGLKITLTDERVDNRKNEYFYEGGIRSYVRHLNRTRE
HCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHCCCCCCEEECCCHHHHHHHHHHHHH
VLHEEPIYIAGERDGIAVEIALQYNDGYTSNIYSFVNNIHTHEGGTHESGFKMALTRIIN
HHHCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
DYARKQQIFKDNDANLTGEDVREGLTAIVSIKHPAPQFEGQTKTKLGNSDARTVTDAVFS
HHHHHHHHHCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCHHCCCCCHHHHHHHHHHH
EQFETFLLEHPAVARKIVEKGMMAARARVAAKKARELTRRKSALEISNLPGKLADCSSRD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PSISELYVVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRAIITAL
CCCEEEEEEECCCCCCCCCCCCCCHHEEEECCCCEEECHHHHHHHHHHCCCCHHHHHHHH
GTGIGEDFDISKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRELIERGYIYIAQPPLY
HCCCCCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCE
KIEQGKQVRYAYNDRQLEKILAELPAQPKPMIQRYKGLGEMNPEQLWETTMNPETRTLLQ
ECCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHH
VSLQDAIDADETFEILMGDKVEPRRQFIEENARYVKNLDI
HHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]