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The map label for this gene is kdc [H]

Identifier: 134302770

GI number: 134302770

Start: 1858937

End: 1860634

Strand: Reverse

Name: kdc [H]

Synonym: FTW_1982

Alternate gene names: 134302770

Gene position: 1860634-1858937 (Counterclockwise)

Preceding gene: 134302781

Following gene: 134302768

Centisome position: 98.01

GC content: 35.51

Gene sequence:

>1698_bases
ATGAAAAAATATATAACTGGAGAAAAACTTTTTGACACCAGTAAGCATGCTACTAATGTTGCTGAATATATCGTTGCAAG
ACTTGTCGATCTAGGAATCACTCATTCTTTTTGTATACCAGGGGATTTCTCATTTGCATTAGATCGTGCACTGATTAATA
ACCCAAAACTTAACAATGTAGTAAATGCTAATGAACTTAATGCTAGCTATGCTGCTGATGGTTACGCAAGAGTCAAAGGA
GCTGCGATTCTTTCGACTACTTATGCAGTTGGTGAACTTAGTGCCCTAAATGGTGTAATGGGCTCAAAAGCTGAAAATCT
TGTTGTTTTTTATCTAGTCGGTAGTCCAGGAGATGGCGCAGTAGGAAAGAAGAGACAGGTTCATCATACATTAGGAGATG
GAGTTTTTGGAAATTTCTTTGATCTATCAGCTTCAGCGGCTTGTGTTTCAGCTGTCATAACACCAGAAAATGCTCGTAGA
GAAATGAATAGAGTCATAGCTGAAGCGTTCAAATATCGCAAACCTGCTTATATAAGCGTATCTTTGGATAGTGGCAATCG
CCCTGTAACCGATATCACACCTGATGATATAGACTGCAGCTATCTAAAAAGTGACTCACATCAGCTTGAATTAGCCACAA
ACCTAGTGCTAGAGCATTTAAGCAAGGCTAAAAAAGTTGTTGCTATCCCAGCCATAAAATTAGATAGGTTTGGAGTAACT
GAAAAAGCTATCAACCTTATAGAAAAGTTAAATATTCCTTTTAGTATTATGCCTCATGATAAAAGTGTCATATCCGAAAC
ACATCCTAACTATGTAGGTTTTTATGCTGGCTTATTATCAGACACAAATACTGCTGAAATAGTTGAGGGCGCAGATCTTA
TAATTAATTTAGGTGATGCGCTATGGTCAGACTTCAATACAGCTGGATTCACCAATAATTTAGATTTAAATAAAGTACTG
AATTTAGGACCTCTATTTGTCGAAGATAACAAAACTTATCTAGCTGACGTTTACTTATCAGAACTACTTGATGCTTTATT
AGAAAAAGTTGAAAGTATAAATTATCGTCCTAATTACTCTAGAATGAAGATACAAGACACTACTATAACGAAAGAAAAGT
TAACTTTAAGAGCACTATATACTCAAGTTTTAAAATTTTTAGAAAATACAGATAGTCTTGTTGTAGAAACCGGTTCCTCT
TCACTTAATATGCCGAAGTTACCACTGCCTGAAGGAGTAAAATATTTCAATCAAACATTATGGGGATCAATCGGTTGGGC
TACTCCTGCTGCACTTGGTGTGGCATTAGCTAATCCAGATTCTAGAACTATATTGATTACAGGTGAAGGGTCCCACCAAT
TAACACTTAATGAACTTGGTGTAATGGGTAGATATAAAATAAATCCTATTATCTTATGCATAAATAATGATGGTTACATG
ATTGAAAGAGCTTTGGAATTAGATCCTAACCCTAGCTATGATGATATTGCGCAACTTAACTACACCAAACTTCCTGAAGC
TTTTGGCTGTAATGATTGGTTAGCTATAAGAGTTAAAACAAGCGAAGAATTAGCCGAAGCTCTTAAAAAAGCTAGAGAGC
ACCAAAGTGGCGTATATATAGAAGTCATTACAGGAAAATATGACTATGGTAATGCTCTTGATTTCTTCAATAATCATTTA
AAGGAAATGTATAGCTAA

Upstream 100 bases:

>100_bases
TTAGCCGCCATAATTAATTATTAGTTGATATTAATATTTTATTCATATAATAATTAATAAAGTTGGGAGTAAAACTTATG
TTCTTAGGGGAAAAGAGATT

Downstream 100 bases:

>100_bases
AATTGACTACTATTCCTCAACATATGCCTTTTCTAACACTTTTCTAAAAGAAATTCCTTGTATAAATATTGAAAGTAATA
CCGCTATATAAATTATCGAA

Product: thiamine pyrophosphate binding domain-containing protein

Products: 2-(indol-3-yl)acetaldehyde; CO2

Alternate protein names: KDC [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MKKYITGEKLFDTSKHATNVAEYIVARLVDLGITHSFCIPGDFSFALDRALINNPKLNNVVNANELNASYAADGYARVKG
AAILSTTYAVGELSALNGVMGSKAENLVVFYLVGSPGDGAVGKKRQVHHTLGDGVFGNFFDLSASAACVSAVITPENARR
EMNRVIAEAFKYRKPAYISVSLDSGNRPVTDITPDDIDCSYLKSDSHQLELATNLVLEHLSKAKKVVAIPAIKLDRFGVT
EKAINLIEKLNIPFSIMPHDKSVISETHPNYVGFYAGLLSDTNTAEIVEGADLIINLGDALWSDFNTAGFTNNLDLNKVL
NLGPLFVEDNKTYLADVYLSELLDALLEKVESINYRPNYSRMKIQDTTITKEKLTLRALYTQVLKFLENTDSLVVETGSS
SLNMPKLPLPEGVKYFNQTLWGSIGWATPAALGVALANPDSRTILITGEGSHQLTLNELGVMGRYKINPIILCINNDGYM
IERALELDPNPSYDDIAQLNYTKLPEAFGCNDWLAIRVKTSEELAEALKKAREHQSGVYIEVITGKYDYGNALDFFNNHL
KEMYS

Sequences:

>Translated_565_residues
MKKYITGEKLFDTSKHATNVAEYIVARLVDLGITHSFCIPGDFSFALDRALINNPKLNNVVNANELNASYAADGYARVKG
AAILSTTYAVGELSALNGVMGSKAENLVVFYLVGSPGDGAVGKKRQVHHTLGDGVFGNFFDLSASAACVSAVITPENARR
EMNRVIAEAFKYRKPAYISVSLDSGNRPVTDITPDDIDCSYLKSDSHQLELATNLVLEHLSKAKKVVAIPAIKLDRFGVT
EKAINLIEKLNIPFSIMPHDKSVISETHPNYVGFYAGLLSDTNTAEIVEGADLIINLGDALWSDFNTAGFTNNLDLNKVL
NLGPLFVEDNKTYLADVYLSELLDALLEKVESINYRPNYSRMKIQDTTITKEKLTLRALYTQVLKFLENTDSLVVETGSS
SLNMPKLPLPEGVKYFNQTLWGSIGWATPAALGVALANPDSRTILITGEGSHQLTLNELGVMGRYKINPIILCINNDGYM
IERALELDPNPSYDDIAQLNYTKLPEAFGCNDWLAIRVKTSEELAEALKKAREHQSGVYIEVITGKYDYGNALDFFNNHL
KEMYS
>Mature_565_residues
MKKYITGEKLFDTSKHATNVAEYIVARLVDLGITHSFCIPGDFSFALDRALINNPKLNNVVNANELNASYAADGYARVKG
AAILSTTYAVGELSALNGVMGSKAENLVVFYLVGSPGDGAVGKKRQVHHTLGDGVFGNFFDLSASAACVSAVITPENARR
EMNRVIAEAFKYRKPAYISVSLDSGNRPVTDITPDDIDCSYLKSDSHQLELATNLVLEHLSKAKKVVAIPAIKLDRFGVT
EKAINLIEKLNIPFSIMPHDKSVISETHPNYVGFYAGLLSDTNTAEIVEGADLIINLGDALWSDFNTAGFTNNLDLNKVL
NLGPLFVEDNKTYLADVYLSELLDALLEKVESINYRPNYSRMKIQDTTITKEKLTLRALYTQVLKFLENTDSLVVETGSS
SLNMPKLPLPEGVKYFNQTLWGSIGWATPAALGVALANPDSRTILITGEGSHQLTLNELGVMGRYKINPIILCINNDGYM
IERALELDPNPSYDDIAQLNYTKLPEAFGCNDWLAIRVKTSEELAEALKKAREHQSGVYIEVITGKYDYGNALDFFNNHL
KEMYS

Specific function: Decarboxylates branched-chain and aromatic alpha-keto acids to aldehydes [H]

COG id: COG3961

COG function: function code GHR; Pyruvate decarboxylase and related thiamine pyrophosphate-requiring enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321524, Length=534, Percent_Identity=33.7078651685393, Blast_Score=266, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6323163, Length=538, Percent_Identity=33.6431226765799, Blast_Score=259, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6323073, Length=516, Percent_Identity=33.7209302325581, Blast_Score=258, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6320123, Length=527, Percent_Identity=30.9297912713473, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6320588, Length=619, Percent_Identity=29.5638126009693, Blast_Score=204, Evalue=2e-53,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012110
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 4.1.1.74

Molecular weight: Translated: 62177; Mature: 62177

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYITGEKLFDTSKHATNVAEYIVARLVDLGITHSFCIPGDFSFALDRALINNPKLNNV
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCCC
VNANELNASYAADGYARVKGAAILSTTYAVGELSALNGVMGSKAENLVVFYLVGSPGDGA
CCCCCCCCCCCCCCHHHHCCEEEEEEHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
VGKKRQVHHTLGDGVFGNFFDLSASAACVSAVITPENARREMNRVIAEAFKYRKPAYISV
CCCCHHHHHHHCCCCCCCHHHCCCHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
SLDSGNRPVTDITPDDIDCSYLKSDSHQLELATNLVLEHLSKAKKVVAIPAIKLDRFGVT
EECCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCEEEEECCEEECCCCCH
EKAINLIEKLNIPFSIMPHDKSVISETHPNYVGFYAGLLSDTNTAEIVEGADLIINLGDA
HHHHHHHHHCCCCEEECCCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCEEEEECCHH
LWSDFNTAGFTNNLDLNKVLNLGPLFVEDNKTYLADVYLSELLDALLEKVESINYRPNYS
HHCCCCCCCCCCCCCHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
RMKIQDTTITKEKLTLRALYTQVLKFLENTDSLVVETGSSSLNMPKLPLPEGVKYFNQTL
EEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
WGSIGWATPAALGVALANPDSRTILITGEGSHQLTLNELGVMGRYKINPIILCINNDGYM
CCCCCCCCHHHHEEEEECCCCCEEEEEECCCCEEEHHHHCCCCEEECCEEEEEECCCCEE
IERALELDPNPSYDDIAQLNYTKLPEAFGCNDWLAIRVKTSEELAEALKKAREHQSGVYI
EEEEEECCCCCCHHHHHHCCHHCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCEEE
EVITGKYDYGNALDFFNNHLKEMYS
EEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKYITGEKLFDTSKHATNVAEYIVARLVDLGITHSFCIPGDFSFALDRALINNPKLNNV
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCCC
VNANELNASYAADGYARVKGAAILSTTYAVGELSALNGVMGSKAENLVVFYLVGSPGDGA
CCCCCCCCCCCCCCHHHHCCEEEEEEHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
VGKKRQVHHTLGDGVFGNFFDLSASAACVSAVITPENARREMNRVIAEAFKYRKPAYISV
CCCCHHHHHHHCCCCCCCHHHCCCHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
SLDSGNRPVTDITPDDIDCSYLKSDSHQLELATNLVLEHLSKAKKVVAIPAIKLDRFGVT
EECCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCEEEEECCEEECCCCCH
EKAINLIEKLNIPFSIMPHDKSVISETHPNYVGFYAGLLSDTNTAEIVEGADLIINLGDA
HHHHHHHHHCCCCEEECCCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCEEEEECCHH
LWSDFNTAGFTNNLDLNKVLNLGPLFVEDNKTYLADVYLSELLDALLEKVESINYRPNYS
HHCCCCCCCCCCCCCHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
RMKIQDTTITKEKLTLRALYTQVLKFLENTDSLVVETGSSSLNMPKLPLPEGVKYFNQTL
EEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
WGSIGWATPAALGVALANPDSRTILITGEGSHQLTLNELGVMGRYKINPIILCINNDGYM
CCCCCCCCHHHHEEEEECCCCCEEEEEECCCCEEEHHHHCCCCEEECCEEEEEECCCCEE
IERALELDPNPSYDDIAQLNYTKLPEAFGCNDWLAIRVKTSEELAEALKKAREHQSGVYI
EEEEEECCCCCCHHHHHHCCHHCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCEEE
EVITGKYDYGNALDFFNNHLKEMYS
EEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(indol-3-yl)pyruvate

Specific reaction: 3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA