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The map label for this gene is ilvE [H]

Identifier: 134302677

GI number: 134302677

Start: 1753964

End: 1754851

Strand: Direct

Name: ilvE [H]

Synonym: FTW_1868

Alternate gene names: 134302677

Gene position: 1753964-1754851 (Clockwise)

Preceding gene: 134302676

Following gene: 134302678

Centisome position: 92.39

GC content: 33.56

Gene sequence:

>888_bases
ATGATAGATAAAATTTGGAAAAATGGAGAAATTATCTCCTATCAAGATGCAAAAGTTGGTATCAATACTCACTCTTTACA
CTATGGTTCATCTGTATTTGAGGGTATAAGAGCATACGATACGCCTAATGGAATTGGAGTACTAAAACTAAGAGAACATA
TGCAAAGATTTATGTACTCAATGAATGTCTTAGGTATGACATGCAAATATACTCTTGATGAGCTATGCCAAGCTGTATTA
GATACTGTCAAAGCTAGCGGTAAAAAATCATGCTATATAAGGCCTTTAGCATATTTTGCTGAAGGAGGAGTCAATGTTTT
ACCTGCGAAAGATCATCCTGTTGATATTACTATTTATTGTATAGATATGGGTAAATATATGGCTGCAGATAAAGTAGATA
TCAAAGTCAGTAAATATATTAGAATTCATCCGCGCTCAACTGTATGTGATGCAAAAATTGGCGGTCATTATGTCAATAGT
ATATTAGCCTCCAGGGAAACTCTTGGTACTCATTATCATGAATCTCTACTTCTTGATGCTGATGGATTTGTAGCTGAAGG
TGCTGCAATGAATGTCTTTTTTGTAAAAGATAATGAAGTTATAACGACTCCTCTAGGCACGATACTAGATGGTATTACTC
GAAGACTTATAATCCAGATTGCTAAGGACCTAGAATATAAAGTTACAGAACGCTTATTTAAAGTTGATGAACTAATAAAT
GCTGATGAAGTATTTTTCTGTGGCACAGCTACTGAAATCACTCCGGTAGCTAGTATTGATGATAATAAGTTAAAACGTTC
TGACTACACAATAACAAACCAAATAAAAGAAGTCTTTGAAAAATTAAAGCAAGGACAGGTTTATAAAGAAGCTCTAACAT
ACATATAA

Upstream 100 bases:

>100_bases
AGCACTCCTCTCCCTCAGCACAAAACTCCTCTGTCTATAACATAAAGACAGTCTCATATAAAAAATACTTAAATTCAATT
ACAAAACACTGGGGTCAATT

Downstream 100 bases:

>100_bases
GGAGCCTAGTATGGATAAGCAAAAAATTTATATTTTTGATACGACTCTTAGAGATGGTCAACAATCTCCAGGAGCAGGCA
TGTCTTTTGAAGACAATATT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MIDKIWKNGEIISYQDAKVGINTHSLHYGSSVFEGIRAYDTPNGIGVLKLREHMQRFMYSMNVLGMTCKYTLDELCQAVL
DTVKASGKKSCYIRPLAYFAEGGVNVLPAKDHPVDITIYCIDMGKYMAADKVDIKVSKYIRIHPRSTVCDAKIGGHYVNS
ILASRETLGTHYHESLLLDADGFVAEGAAMNVFFVKDNEVITTPLGTILDGITRRLIIQIAKDLEYKVTERLFKVDELIN
ADEVFFCGTATEITPVASIDDNKLKRSDYTITNQIKEVFEKLKQGQVYKEALTYI

Sequences:

>Translated_295_residues
MIDKIWKNGEIISYQDAKVGINTHSLHYGSSVFEGIRAYDTPNGIGVLKLREHMQRFMYSMNVLGMTCKYTLDELCQAVL
DTVKASGKKSCYIRPLAYFAEGGVNVLPAKDHPVDITIYCIDMGKYMAADKVDIKVSKYIRIHPRSTVCDAKIGGHYVNS
ILASRETLGTHYHESLLLDADGFVAEGAAMNVFFVKDNEVITTPLGTILDGITRRLIIQIAKDLEYKVTERLFKVDELIN
ADEVFFCGTATEITPVASIDDNKLKRSDYTITNQIKEVFEKLKQGQVYKEALTYI
>Mature_295_residues
MIDKIWKNGEIISYQDAKVGINTHSLHYGSSVFEGIRAYDTPNGIGVLKLREHMQRFMYSMNVLGMTCKYTLDELCQAVL
DTVKASGKKSCYIRPLAYFAEGGVNVLPAKDHPVDITIYCIDMGKYMAADKVDIKVSKYIRIHPRSTVCDAKIGGHYVNS
ILASRETLGTHYHESLLLDADGFVAEGAAMNVFFVKDNEVITTPLGTILDGITRRLIIQIAKDLEYKVTERLFKVDELIN
ADEVFFCGTATEITPVASIDDNKLKRSDYTITNQIKEVFEKLKQGQVYKEALTYI

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=282, Percent_Identity=28.7234042553192, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI38176287, Length=282, Percent_Identity=28.7234042553192, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI296010906, Length=282, Percent_Identity=28.7234042553192, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI48994963, Length=290, Percent_Identity=38.9655172413793, Blast_Score=211, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17565728, Length=278, Percent_Identity=25.8992805755396, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322608, Length=289, Percent_Identity=28.0276816608997, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322002, Length=289, Percent_Identity=26.9896193771626, Blast_Score=83, Evalue=6e-17,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33146; Mature: 33146

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKIWKNGEIISYQDAKVGINTHSLHYGSSVFEGIRAYDTPNGIGVLKLREHMQRFMYS
CCCCCCCCCCEEEECCCEECCCCCCEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
MNVLGMTCKYTLDELCQAVLDTVKASGKKSCYIRPLAYFAEGGVNVLPAKDHPVDITIYC
HHHHCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCCCCEEEECCCCCEEEEEEE
IDMGKYMAADKVDIKVSKYIRIHPRSTVCDAKIGGHYVNSILASRETLGTHYHESLLLDA
EECCCCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECC
DGFVAEGAAMNVFFVKDNEVITTPLGTILDGITRRLIIQIAKDLEYKVTERLFKVDELIN
CCCEECCCEEEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADEVFFCGTATEITPVASIDDNKLKRSDYTITNQIKEVFEKLKQGQVYKEALTYI
CCCEEEECCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MIDKIWKNGEIISYQDAKVGINTHSLHYGSSVFEGIRAYDTPNGIGVLKLREHMQRFMYS
CCCCCCCCCCEEEECCCEECCCCCCEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
MNVLGMTCKYTLDELCQAVLDTVKASGKKSCYIRPLAYFAEGGVNVLPAKDHPVDITIYC
HHHHCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCCCCEEEECCCCCEEEEEEE
IDMGKYMAADKVDIKVSKYIRIHPRSTVCDAKIGGHYVNSILASRETLGTHYHESLLLDA
EECCCCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECC
DGFVAEGAAMNVFFVKDNEVITTPLGTILDGITRRLIIQIAKDLEYKVTERLFKVDELIN
CCCEECCCEEEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADEVFFCGTATEITPVASIDDNKLKRSDYTITNQIKEVFEKLKQGQVYKEALTYI
CCCEEEECCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]