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The map label for this gene is ppdK [H]

Identifier: 134302676

GI number: 134302676

Start: 1750931

End: 1753564

Strand: Direct

Name: ppdK [H]

Synonym: FTW_1867

Alternate gene names: 134302676

Gene position: 1750931-1753564 (Clockwise)

Preceding gene: 134302675

Following gene: 134302677

Centisome position: 92.23

GC content: 37.21

Gene sequence:

>2634_bases
ATGTCGAAGTTTGTCTATGCCTTTAGCGAAGGTAATAAGTCTATGCGTGATTTACTAGGTGGAAAAGGTGCAAATCTTAG
TGAAATGCTAAATAGTGGTCTACCTGTGCCAGATGGATTTACCGTAACTACAGAAGCATGTCTTAAGTATTATGATGATC
GCCAAAAGTTAAGTAATGAAGTTAAAGAACAAATATTTGCTCATATCAAAGATTTAGAGAAAAGAACTGGTAAAACTTTT
GGTGGTGGCAATAATCCTCTACTAGTATCAGTACGCTCTGGTGCAAGAGTTTCTATGCCTGGGATGATGGATACTGTCCT
TAATTTAGGACTAAATGATACTGTTGCTCAAGCTATGGTAGCAAAGACAAACAATGAGCAATTTGTTTATGATAGCTATA
GAAGATTCATAATGATGTTTGCAGATGTTGTTATGGATTGTGAGAAGAAACCTTTTGATAAAGTTTTAGAAGATAAAAAA
GCTAAAAGAAAGGTAAAAAATGATTGTGATTTACCAGCTCAAGATTATAAAGATATAGTTACAGAATATAAGAAAATTTA
TAAAGATTTAGTCGGTAAGGACTTCCCTACTGATCCAATTGAGCAACTTCTGGCTGCTGTAGAAGCCGTTTTTAAATCAT
GGAATGCCGAGAGAGCAATCATCTATAGAGAGATAAATAATATTTCAAATAACTGGGGTACAGCTGTTAATGTCCAAGAA
ATGGTCTATGGTAACTCTGGTAATAATTCTGGGACAGGAGTAGCATTTACTAGAAACCCATCGACTGGTGAAAATGAGCT
TTTTGGTGAATATCTAATTAATGCGCAGGGAGAAGATGTTGTAGCTGGTATCAGAACCCCAGCGCATATCTCAACTTTAA
AAGATAAAATGCCAGAAGTATTTAATGACTTTGTGAAGATTGCTAAAAACTTAGAAAAAGTTTACAAAGACATGCAAGAT
ATGGAGTTTACTATCGAAGATGGTAAGCTTTTCATGCTACAAACAAGAAATGGTAAAAGAACTGCAAAAGCAGCTTTGAA
AATAGCAGTTGATATGGCTAAAGAAGGCTTAATCACAAATGAAGAAGCTGTAATGATGGTAGAGCCTCATTTATTAGAGC
AACTACTACATCCTAAATTTGATGAGAAAGCTCTAGCTTCTAAACGCCCTCTTGGTTCAGCTCTAGGTGCGTCTCCAGGC
GCTGCTAGTGGTAGAATATACTTTGATGTTGAATCACTACTTGCAGCAAAAGCACGTGGTGAAGAAAAAACTATTCTTGT
TAGGATTGAAACCTCTCCTGAAGATATCGCGGGGATGAATGCTTGTAATGGTATCTTAACTCTACGTGGTGGTATGACAT
CTCATGCTGCTGTTGTTGCTCGTGGTATGGGTAAATGTTGTGTGTCTGGTTTAGAATCAGCAAGAATTGATGAAGAAAAG
AAAACTATCACATTTGAGAGGGGCCAAGTATTTACAGAAGGTGATTATTTATCTCTTGATGGTACCAAAGGTACTGTCTA
TAGAGGTATTATTAAAACTGTAGATCCTGAAGTTACTAAAGATTTTGAAGACTTTATGAAGTTCGTCGATAGTGTACGTA
AACTTCGTGTTAGATGTAATGCCGATACTTATAAAGATGCCTCAATTGCAAGAGCATTTGGCGCGGAAGGTATTGGTTTA
TGTCGTACTGAGCATATGTTCTTTGAGGAAGATCGTATCTCTTATGTTAGACAGATGATCCTAGCTAAAGATAAAAAAGA
AAGACAAAGAGCTTTAGATAAACTACTTCCTGTACAGCAACAAGATTTTGAAGAGCTTTTTGAAGCGATGGATAATCTAG
CTGTAACGATTAGATTTATCGATCCGCCTTTACATGAGTTTTTACCTCACGAGGTGGATGAGATTGATGATCTAGCTCGT
GAGTTTAATATTAGCTATAGTGAACTAGAAGCACGTATTGAAGCACTTAGCGAAGTAAACCCTATGATGGGTCATCGCGG
CTGTAGACTTGCTGTAACGTATCCTGAAATCATTGAGATGCAAACTAAAGCTATCATCTATGCAGCGATATCAAGTAAAC
GTATGGGTATAGAAGTTAAGCCAGAGCTTATGATTCCTCTAGTTAGTACTCTTGGTGAGTTTAAACTCTTAAGTGGTATT
GTCAGAGAAGTTGCAGACACTATCCTAAAGCGTGAAAAAATTGATATTGATTACAAAGTTGGGGTAATGCTTGAAACTCC
ACGTGGTGCTATCGGCGCTGGTATGCTTGCAGCTGCTGGTAGTGAATTTTTCTCATTTGGTACAAATGATTTGACTCAGA
TGACTTTTGGTTTCAGTAGAGATGATGCTAATAAGTTTATTAAAGACTATCTTGAAAAAGGCATACTAAGCTTTGATCCA
TTTGCTAGGCTAGATATTAAAGGTGTTGGTAAACTAATGCAAATAGCCAAAGAAGGCGTCAAGGCTGTTAATCCTAATGC
TAAGATGGGTATATGTGGCGAGCATGGTGGCGAACCGTATTCTGTTGGTTTCTGTCATGATTTGGATCTTGACTATGTAT
CTTGCTCTCCTTTTAGAGTTCCTATCGCAAGATTAGCGGCTGCTCAAGCTAAAATATATGCAGACCGGAGATAA

Upstream 100 bases:

>100_bases
CTTTAATATATCTATCAAAATGTGATAGGATAAGACTCAAGTACGCTTGAGAGTGTACTGTTTTCAAATTAATTTATAAA
AAAACTTGAGAGGAGTTTTT

Downstream 100 bases:

>100_bases
TAATCATTTCTATTGTTTAATCTCTTGATTTTAAAAAATCATTAATACGCTATAATCTAGGTTTACACTAAAATATTATA
TAGCTACAATAACTATATCT

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 877; Mature: 876

Protein sequence:

>877_residues
MSKFVYAFSEGNKSMRDLLGGKGANLSEMLNSGLPVPDGFTVTTEACLKYYDDRQKLSNEVKEQIFAHIKDLEKRTGKTF
GGGNNPLLVSVRSGARVSMPGMMDTVLNLGLNDTVAQAMVAKTNNEQFVYDSYRRFIMMFADVVMDCEKKPFDKVLEDKK
AKRKVKNDCDLPAQDYKDIVTEYKKIYKDLVGKDFPTDPIEQLLAAVEAVFKSWNAERAIIYREINNISNNWGTAVNVQE
MVYGNSGNNSGTGVAFTRNPSTGENELFGEYLINAQGEDVVAGIRTPAHISTLKDKMPEVFNDFVKIAKNLEKVYKDMQD
MEFTIEDGKLFMLQTRNGKRTAKAALKIAVDMAKEGLITNEEAVMMVEPHLLEQLLHPKFDEKALASKRPLGSALGASPG
AASGRIYFDVESLLAAKARGEEKTILVRIETSPEDIAGMNACNGILTLRGGMTSHAAVVARGMGKCCVSGLESARIDEEK
KTITFERGQVFTEGDYLSLDGTKGTVYRGIIKTVDPEVTKDFEDFMKFVDSVRKLRVRCNADTYKDASIARAFGAEGIGL
CRTEHMFFEEDRISYVRQMILAKDKKERQRALDKLLPVQQQDFEELFEAMDNLAVTIRFIDPPLHEFLPHEVDEIDDLAR
EFNISYSELEARIEALSEVNPMMGHRGCRLAVTYPEIIEMQTKAIIYAAISSKRMGIEVKPELMIPLVSTLGEFKLLSGI
VREVADTILKREKIDIDYKVGVMLETPRGAIGAGMLAAAGSEFFSFGTNDLTQMTFGFSRDDANKFIKDYLEKGILSFDP
FARLDIKGVGKLMQIAKEGVKAVNPNAKMGICGEHGGEPYSVGFCHDLDLDYVSCSPFRVPIARLAAAQAKIYADRR

Sequences:

>Translated_877_residues
MSKFVYAFSEGNKSMRDLLGGKGANLSEMLNSGLPVPDGFTVTTEACLKYYDDRQKLSNEVKEQIFAHIKDLEKRTGKTF
GGGNNPLLVSVRSGARVSMPGMMDTVLNLGLNDTVAQAMVAKTNNEQFVYDSYRRFIMMFADVVMDCEKKPFDKVLEDKK
AKRKVKNDCDLPAQDYKDIVTEYKKIYKDLVGKDFPTDPIEQLLAAVEAVFKSWNAERAIIYREINNISNNWGTAVNVQE
MVYGNSGNNSGTGVAFTRNPSTGENELFGEYLINAQGEDVVAGIRTPAHISTLKDKMPEVFNDFVKIAKNLEKVYKDMQD
MEFTIEDGKLFMLQTRNGKRTAKAALKIAVDMAKEGLITNEEAVMMVEPHLLEQLLHPKFDEKALASKRPLGSALGASPG
AASGRIYFDVESLLAAKARGEEKTILVRIETSPEDIAGMNACNGILTLRGGMTSHAAVVARGMGKCCVSGLESARIDEEK
KTITFERGQVFTEGDYLSLDGTKGTVYRGIIKTVDPEVTKDFEDFMKFVDSVRKLRVRCNADTYKDASIARAFGAEGIGL
CRTEHMFFEEDRISYVRQMILAKDKKERQRALDKLLPVQQQDFEELFEAMDNLAVTIRFIDPPLHEFLPHEVDEIDDLAR
EFNISYSELEARIEALSEVNPMMGHRGCRLAVTYPEIIEMQTKAIIYAAISSKRMGIEVKPELMIPLVSTLGEFKLLSGI
VREVADTILKREKIDIDYKVGVMLETPRGAIGAGMLAAAGSEFFSFGTNDLTQMTFGFSRDDANKFIKDYLEKGILSFDP
FARLDIKGVGKLMQIAKEGVKAVNPNAKMGICGEHGGEPYSVGFCHDLDLDYVSCSPFRVPIARLAAAQAKIYADRR
>Mature_876_residues
SKFVYAFSEGNKSMRDLLGGKGANLSEMLNSGLPVPDGFTVTTEACLKYYDDRQKLSNEVKEQIFAHIKDLEKRTGKTFG
GGNNPLLVSVRSGARVSMPGMMDTVLNLGLNDTVAQAMVAKTNNEQFVYDSYRRFIMMFADVVMDCEKKPFDKVLEDKKA
KRKVKNDCDLPAQDYKDIVTEYKKIYKDLVGKDFPTDPIEQLLAAVEAVFKSWNAERAIIYREINNISNNWGTAVNVQEM
VYGNSGNNSGTGVAFTRNPSTGENELFGEYLINAQGEDVVAGIRTPAHISTLKDKMPEVFNDFVKIAKNLEKVYKDMQDM
EFTIEDGKLFMLQTRNGKRTAKAALKIAVDMAKEGLITNEEAVMMVEPHLLEQLLHPKFDEKALASKRPLGSALGASPGA
ASGRIYFDVESLLAAKARGEEKTILVRIETSPEDIAGMNACNGILTLRGGMTSHAAVVARGMGKCCVSGLESARIDEEKK
TITFERGQVFTEGDYLSLDGTKGTVYRGIIKTVDPEVTKDFEDFMKFVDSVRKLRVRCNADTYKDASIARAFGAEGIGLC
RTEHMFFEEDRISYVRQMILAKDKKERQRALDKLLPVQQQDFEELFEAMDNLAVTIRFIDPPLHEFLPHEVDEIDDLARE
FNISYSELEARIEALSEVNPMMGHRGCRLAVTYPEIIEMQTKAIIYAAISSKRMGIEVKPELMIPLVSTLGEFKLLSGIV
REVADTILKREKIDIDYKVGVMLETPRGAIGAGMLAAAGSEFFSFGTNDLTQMTFGFSRDDANKFIKDYLEKGILSFDPF
ARLDIKGVGKLMQIAKEGVKAVNPNAKMGICGEHGGEPYSVGFCHDLDLDYVSCSPFRVPIARLAAAQAKIYADRR

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=462, Percent_Identity=25.5411255411255, Blast_Score=102, Evalue=2e-22,
Organism=Escherichia coli, GI1789193, Length=449, Percent_Identity=22.9398663697105, Blast_Score=77, Evalue=7e-15,
Organism=Escherichia coli, GI48994992, Length=453, Percent_Identity=23.6203090507726, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97596; Mature: 97465

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFVYAFSEGNKSMRDLLGGKGANLSEMLNSGLPVPDGFTVTTEACLKYYDDRQKLSNE
CCCEEEEEECCCHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
VKEQIFAHIKDLEKRTGKTFGGGNNPLLVSVRSGARVSMPGMMDTVLNLGLNDTVAQAMV
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHHHH
AKTNNEQFVYDSYRRFIMMFADVVMDCEKKPFDKVLEDKKAKRKVKNDCDLPAQDYKDIV
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TEYKKIYKDLVGKDFPTDPIEQLLAAVEAVFKSWNAERAIIYREINNISNNWGTAVNVQE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCEEEHHH
MVYGNSGNNSGTGVAFTRNPSTGENELFGEYLINAQGEDVVAGIRTPAHISTLKDKMPEV
HHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHH
FNDFVKIAKNLEKVYKDMQDMEFTIEDGKLFMLQTRNGKRTAKAALKIAVDMAKEGLITN
HHHHHHHHHHHHHHHHHHHHCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCC
EEAVMMVEPHLLEQLLHPKFDEKALASKRPLGSALGASPGAASGRIYFDVESLLAAKARG
CCEEEEECHHHHHHHHCCCCCHHHHHHCCCCCHHHCCCCCCCCCEEEEEHHHHHHHHCCC
EEKTILVRIETSPEDIAGMNACNGILTLRGGMTSHAAVVARGMGKCCVSGLESARIDEEK
CCCEEEEEEECCCHHHCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
KTITFERGQVFTEGDYLSLDGTKGTVYRGIIKTVDPEVTKDFEDFMKFVDSVRKLRVRCN
HEEEEECCEEEECCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
ADTYKDASIARAFGAEGIGLCRTEHMFFEEDRISYVRQMILAKDKKERQRALDKLLPVQQ
CCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCH
QDFEELFEAMDNLAVTIRFIDPPLHEFLPHEVDEIDDLAREFNISYSELEARIEALSEVN
HHHHHHHHHHCCEEEEEEEECCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
PMMGHRGCRLAVTYPEIIEMQTKAIIYAAISSKRMGIEVKPELMIPLVSTLGEFKLLSGI
HHHCCCCCEEEEECHHHHHHHHHHEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHH
VREVADTILKREKIDIDYKVGVMLETPRGAIGAGMLAAAGSEFFSFGTNDLTQMTFGFSR
HHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHCCCCCHHHHHCCCCC
DDANKFIKDYLEKGILSFDPFARLDIKGVGKLMQIAKEGVKAVNPNAKMGICGEHGGEPY
CHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEE
SVGFCHDLDLDYVSCSPFRVPIARLAAAQAKIYADRR
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKFVYAFSEGNKSMRDLLGGKGANLSEMLNSGLPVPDGFTVTTEACLKYYDDRQKLSNE
CCEEEEEECCCHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
VKEQIFAHIKDLEKRTGKTFGGGNNPLLVSVRSGARVSMPGMMDTVLNLGLNDTVAQAMV
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHHHH
AKTNNEQFVYDSYRRFIMMFADVVMDCEKKPFDKVLEDKKAKRKVKNDCDLPAQDYKDIV
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TEYKKIYKDLVGKDFPTDPIEQLLAAVEAVFKSWNAERAIIYREINNISNNWGTAVNVQE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCEEEHHH
MVYGNSGNNSGTGVAFTRNPSTGENELFGEYLINAQGEDVVAGIRTPAHISTLKDKMPEV
HHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHH
FNDFVKIAKNLEKVYKDMQDMEFTIEDGKLFMLQTRNGKRTAKAALKIAVDMAKEGLITN
HHHHHHHHHHHHHHHHHHHHCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCC
EEAVMMVEPHLLEQLLHPKFDEKALASKRPLGSALGASPGAASGRIYFDVESLLAAKARG
CCEEEEECHHHHHHHHCCCCCHHHHHHCCCCCHHHCCCCCCCCCEEEEEHHHHHHHHCCC
EEKTILVRIETSPEDIAGMNACNGILTLRGGMTSHAAVVARGMGKCCVSGLESARIDEEK
CCCEEEEEEECCCHHHCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
KTITFERGQVFTEGDYLSLDGTKGTVYRGIIKTVDPEVTKDFEDFMKFVDSVRKLRVRCN
HEEEEECCEEEECCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
ADTYKDASIARAFGAEGIGLCRTEHMFFEEDRISYVRQMILAKDKKERQRALDKLLPVQQ
CCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCH
QDFEELFEAMDNLAVTIRFIDPPLHEFLPHEVDEIDDLAREFNISYSELEARIEALSEVN
HHHHHHHHHHCCEEEEEEEECCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
PMMGHRGCRLAVTYPEIIEMQTKAIIYAAISSKRMGIEVKPELMIPLVSTLGEFKLLSGI
HHHCCCCCEEEEECHHHHHHHHHHEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHH
VREVADTILKREKIDIDYKVGVMLETPRGAIGAGMLAAAGSEFFSFGTNDLTQMTFGFSR
HHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHCCCCCHHHHHCCCCC
DDANKFIKDYLEKGILSFDPFARLDIKGVGKLMQIAKEGVKAVNPNAKMGICGEHGGEPY
CHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEE
SVGFCHDLDLDYVSCSPFRVPIARLAAAQAKIYADRR
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]