Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 134302445

GI number: 134302445

Start: 1497061

End: 1497843

Strand: Direct

Name: birA [H]

Synonym: FTW_1593

Alternate gene names: 134302445

Gene position: 1497061-1497843 (Clockwise)

Preceding gene: 134302444

Following gene: 134302446

Centisome position: 78.86

GC content: 27.71

Gene sequence:

>783_bases
ATGAAAAATCACCGCCTCATAATAACACAACTTAACAAATATATAGATGATATAAAAGTTGAATATTTTCCAACTATAGA
CTCAACAAATGACTATTTCTTAGCAACAAATTTCACACATAAATATCATTTTTGTTATGTGGATAAGCAAACAAAAGGTC
GTGGTCGTCGTGGCGATAAATGGATTTCTGAAGATAAAGATAATATCTACTCAACATTAGCGTTTCATTGTGATTTTGCA
ATCACAGCTGACTCACTTAAAAGTGTCAAAATAGCTCTAGGAGTCTTAGCAGCTATCAAAAAATATATTCCCAAAAATCT
ACAACAATACCTAAAGATAAAATTACCAAATGATATTTATTTTCAAGATCAAAAACTCGCTGGGATACTAATAGAAACAA
AAAATATCAAAAAAGATAGTTTTGACATTATTATAGGAGTAGGTATAAATGTCAATATGACAAATACTGATGAAAATATT
GATCGCGAGTGGACATCTTTATCAAACATAAATAATAAACAATTAAACTCTTCTAGAATCATAGTTGATTTAGTTAAAAG
CATTATTGATAGCTTTGATATGAATGATGCCACTGCACTAGCACAATTAATTAGTTATGATTATATTTTAGATAAGAGAA
TTACTTTTAACTATGCAGATCAAAGTTACCAGGGTATCGCCAAAGGTATTTCTGAGGATCTGAAACTAAAAATTATAGAT
GAAAATAATAATTATTGTGAATTTGAACTAGCTAATATTAATAAAATTAGAGTTATAAAATGA

Upstream 100 bases:

>100_bases
AGCTAATTATCGGCGACTGTATAAAAGCCTGCTATGAGTTACAAAAAGAAGAATTCTTAGGACAAATAAACCTATCACTT
AATATTAAAACTATCGAAAA

Downstream 100 bases:

>100_bases
GTTTAGAAAATATTAAAAAACGTGCTGAATATCTTGCAAAGATTCGTAACTTTTTCAAAGATTTAGATGTCTTAGAAGTA
GATACGCCGTTAGCATATGA

Product: biotin-(acetyl-CoA-carboxylase) ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKNHRLIITQLNKYIDDIKVEYFPTIDSTNDYFLATNFTHKYHFCYVDKQTKGRGRRGDKWISEDKDNIYSTLAFHCDFA
ITADSLKSVKIALGVLAAIKKYIPKNLQQYLKIKLPNDIYFQDQKLAGILIETKNIKKDSFDIIIGVGINVNMTNTDENI
DREWTSLSNINNKQLNSSRIIVDLVKSIIDSFDMNDATALAQLISYDYILDKRITFNYADQSYQGIAKGISEDLKLKIID
ENNNYCEFELANINKIRVIK

Sequences:

>Translated_260_residues
MKNHRLIITQLNKYIDDIKVEYFPTIDSTNDYFLATNFTHKYHFCYVDKQTKGRGRRGDKWISEDKDNIYSTLAFHCDFA
ITADSLKSVKIALGVLAAIKKYIPKNLQQYLKIKLPNDIYFQDQKLAGILIETKNIKKDSFDIIIGVGINVNMTNTDENI
DREWTSLSNINNKQLNSSRIIVDLVKSIIDSFDMNDATALAQLISYDYILDKRITFNYADQSYQGIAKGISEDLKLKIID
ENNNYCEFELANINKIRVIK
>Mature_260_residues
MKNHRLIITQLNKYIDDIKVEYFPTIDSTNDYFLATNFTHKYHFCYVDKQTKGRGRRGDKWISEDKDNIYSTLAFHCDFA
ITADSLKSVKIALGVLAAIKKYIPKNLQQYLKIKLPNDIYFQDQKLAGILIETKNIKKDSFDIIIGVGINVNMTNTDENI
DREWTSLSNINNKQLNSSRIIVDLVKSIIDSFDMNDATALAQLISYDYILDKRITFNYADQSYQGIAKGISEDLKLKIID
ENNNYCEFELANINKIRVIK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=236, Percent_Identity=29.6610169491525, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 30065; Mature: 30065

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNHRLIITQLNKYIDDIKVEYFPTIDSTNDYFLATNFTHKYHFCYVDKQTKGRGRRGDK
CCCCEEEHHHHHHHHHHCEEEEEECCCCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCC
WISEDKDNIYSTLAFHCDFAITADSLKSVKIALGVLAAIKKYIPKNLQQYLKIKLPNDIY
CCCCCHHHHHHHHHEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEE
FQDQKLAGILIETKNIKKDSFDIIIGVGINVNMTNTDENIDREWTSLSNINNKQLNSSRI
EECCCEEEEEEEECCCCCCCCEEEEEECEEEEECCCCCCCHHHHHHHHCCCCCCCCCCHH
IVDLVKSIIDSFDMNDATALAQLISYDYILDKRITFNYADQSYQGIAKGISEDLKLKIID
HHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCEEEEEECCCHHHHHHHCCCCCEEEEEEE
ENNNYCEFELANINKIRVIK
CCCCEEEEEECCCCEEEEEC
>Mature Secondary Structure
MKNHRLIITQLNKYIDDIKVEYFPTIDSTNDYFLATNFTHKYHFCYVDKQTKGRGRRGDK
CCCCEEEHHHHHHHHHHCEEEEEECCCCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCC
WISEDKDNIYSTLAFHCDFAITADSLKSVKIALGVLAAIKKYIPKNLQQYLKIKLPNDIY
CCCCCHHHHHHHHHEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEE
FQDQKLAGILIETKNIKKDSFDIIIGVGINVNMTNTDENIDREWTSLSNINNKQLNSSRI
EECCCEEEEEEEECCCCCCCCEEEEEECEEEEECCCCCCCHHHHHHHHCCCCCCCCCCHH
IVDLVKSIIDSFDMNDATALAQLISYDYILDKRITFNYADQSYQGIAKGISEDLKLKIID
HHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCEEEEEECCCHHHHHHHCCCCCEEEEEEE
ENNNYCEFELANINKIRVIK
CCCCEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]