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The map label for this gene is yjeA [H]

Identifier: 134302446

GI number: 134302446

Start: 1497840

End: 1498763

Strand: Direct

Name: yjeA [H]

Synonym: FTW_1594

Alternate gene names: 134302446

Gene position: 1497840-1498763 (Clockwise)

Preceding gene: 134302445

Following gene: 134302456

Centisome position: 78.9

GC content: 30.84

Gene sequence:

>924_bases
ATGAGTTTAGAAAATATTAAAAAACGTGCTGAATATCTTGCAAAGATTCGTAACTTTTTCAAAGATTTAGATGTCTTAGA
AGTAGATACGCCGTTAGCATATGATTATGCAGTTACCGATCCATTTATAGATGTATTTAGCATAAATACAGTGGCTGGAA
AAAAATATATACAAAGTTCTCCTGAATATGCAATGAAAAGATTACTTGCTGCTGGTAGTGGTAGTATTTATCAAATTTGT
AAAGCTTTTCGCGATGAGCCTTGTGGTAAGTTACACAATCATGAGTTTACAATGATAGAATGGTATCGAGTTGGCATAGA
CTACTATCAACTAATGCAGGAAATGCAAAAGTTATTTACTATGCTCAAAGCAAATTTAGAATTTATCTATTTAAGCTATC
AAGAAGTATTTGAAAAATACTACAATATTAATCCTCATGATATTTGTTTAGCTGAGCTACAAGCAATAGTTAAAGAAAAT
GTTGGTGAAATTCAAGGCTTAAGAAATCCAACTATAGCAGATTGCTTAGATATTCTTTTTAGCTATAAAATTGAAAAAAA
CCTCAATCAACAAAATACTATTTATTTTATCTATGACTACACAATTCACCAATCAGCCTTAGCTAGAAAAGTTAGAGATA
AAAACAGCCAATTAGTTGCCGCAAGATTTGAAGTTTTTTGTAACGGTATAGAACTAGCAAATGGCTACTATGAACTTATA
GATAAAAAGGAGCAATCAAAACGCTTTGAGAATGATCTTGCAACGAGAAAACAACAACAAAAGGCTCTTTTAGATATTGA
CACTAAACTTCTAGAGTGTTTGGAAAATATTCCTGAATGTTCTGGAGTTGCTTTAGGATTTGATAGGCTACTTATGAGTT
TAGAGGGTATTAGCAATATAAAAAATCTAACTATCTTAGATTAG

Upstream 100 bases:

>100_bases
CCAAAGGTATTTCTGAGGATCTGAAACTAAAAATTATAGATGAAAATAATAATTATTGTGAATTTGAACTAGCTAATATT
AATAAAATTAGAGTTATAAA

Downstream 100 bases:

>100_bases
ATTACTAATATTTATGTAACGTAACTTGATTTATCGCAAACTCTATATCTAAATCATTTAATTCTTTGGCAATATTTATA
AAAATTTCATCCTGAACATT

Product: tRNA synthetase, class II (D, K and N)

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSLENIKKRAEYLAKIRNFFKDLDVLEVDTPLAYDYAVTDPFIDVFSINTVAGKKYIQSSPEYAMKRLLAAGSGSIYQIC
KAFRDEPCGKLHNHEFTMIEWYRVGIDYYQLMQEMQKLFTMLKANLEFIYLSYQEVFEKYYNINPHDICLAELQAIVKEN
VGEIQGLRNPTIADCLDILFSYKIEKNLNQQNTIYFIYDYTIHQSALARKVRDKNSQLVAARFEVFCNGIELANGYYELI
DKKEQSKRFENDLATRKQQQKALLDIDTKLLECLENIPECSGVALGFDRLLMSLEGISNIKNLTILD

Sequences:

>Translated_307_residues
MSLENIKKRAEYLAKIRNFFKDLDVLEVDTPLAYDYAVTDPFIDVFSINTVAGKKYIQSSPEYAMKRLLAAGSGSIYQIC
KAFRDEPCGKLHNHEFTMIEWYRVGIDYYQLMQEMQKLFTMLKANLEFIYLSYQEVFEKYYNINPHDICLAELQAIVKEN
VGEIQGLRNPTIADCLDILFSYKIEKNLNQQNTIYFIYDYTIHQSALARKVRDKNSQLVAARFEVFCNGIELANGYYELI
DKKEQSKRFENDLATRKQQQKALLDIDTKLLECLENIPECSGVALGFDRLLMSLEGISNIKNLTILD
>Mature_306_residues
SLENIKKRAEYLAKIRNFFKDLDVLEVDTPLAYDYAVTDPFIDVFSINTVAGKKYIQSSPEYAMKRLLAAGSGSIYQICK
AFRDEPCGKLHNHEFTMIEWYRVGIDYYQLMQEMQKLFTMLKANLEFIYLSYQEVFEKYYNINPHDICLAELQAIVKENV
GEIQGLRNPTIADCLDILFSYKIEKNLNQQNTIYFIYDYTIHQSALARKVRDKNSQLVAARFEVFCNGIELANGYYELID
KKEQSKRFENDLATRKQQQKALLDIDTKLLECLENIPECSGVALGFDRLLMSLEGISNIKNLTILD

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87082379, Length=313, Percent_Identity=38.0191693290735, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI1789256, Length=327, Percent_Identity=26.9113149847095, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790571, Length=320, Percent_Identity=26.875, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 35648; Mature: 35517

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLENIKKRAEYLAKIRNFFKDLDVLEVDTPLAYDYAVTDPFIDVFSINTVAGKKYIQSS
CCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECEECCCCCHHHHCCCHHHHHHHHHCC
PEYAMKRLLAAGSGSIYQICKAFRDEPCGKLHNHEFTMIEWYRVGIDYYQLMQEMQKLFT
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCHHHHHHHHHHHHHHH
MLKANLEFIYLSYQEVFEKYYNINPHDICLAELQAIVKENVGEIQGLRNPTIADCLDILF
HHHCCHHEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SYKIEKNLNQQNTIYFIYDYTIHQSALARKVRDKNSQLVAARFEVFCNGIELANGYYELI
HHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DKKEQSKRFENDLATRKQQQKALLDIDTKLLECLENIPECSGVALGFDRLLMSLEGISNI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
KNLTILD
CCCEECC
>Mature Secondary Structure 
SLENIKKRAEYLAKIRNFFKDLDVLEVDTPLAYDYAVTDPFIDVFSINTVAGKKYIQSS
CHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECEECCCCCHHHHCCCHHHHHHHHHCC
PEYAMKRLLAAGSGSIYQICKAFRDEPCGKLHNHEFTMIEWYRVGIDYYQLMQEMQKLFT
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCHHHHHHHHHHHHHHH
MLKANLEFIYLSYQEVFEKYYNINPHDICLAELQAIVKENVGEIQGLRNPTIADCLDILF
HHHCCHHEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SYKIEKNLNQQNTIYFIYDYTIHQSALARKVRDKNSQLVAARFEVFCNGIELANGYYELI
HHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DKKEQSKRFENDLATRKQQQKALLDIDTKLLECLENIPECSGVALGFDRLLMSLEGISNI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
KNLTILD
CCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA