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The map label for this gene is dacB2 [H]

Identifier: 134302386

GI number: 134302386

Start: 1420058

End: 1421452

Strand: Direct

Name: dacB2 [H]

Synonym: FTW_1514

Alternate gene names: 134302386

Gene position: 1420058-1421452 (Clockwise)

Preceding gene: 134302385

Following gene: 134302387

Centisome position: 74.8

GC content: 30.11

Gene sequence:

>1395_bases
ATGCCCATAAAATTACTTAAATCATTATTATTATTTTTTATATTATCAGTCTTTATAGCATATCCACTATCAGTAAATTC
AATAGAATTAAATCAAGTATTAGACAACTCTATAGAGCAGAATAACCTTCAGTCAGCTCAGATTGCAGTTAAGATTATTG
ACTCTGAAAAAAATACTGTGATTTTGTCAAAAAATGCCGATAAAAACTTCATACCTGCTAGCAATACTAAATTACTTACT
GGAATTGCAGGGTTATTATTTCTTGGTAAAGATTTTAGGTTTGAAACTAAGCTATATTATGACAAAATTAACAATCAAAG
TATTGATAACCTTTATATTGAGTTTTCAGGTGATCCAAGTTTTACGCGCGAAGACTTACATAAACTCTTGATAAGCTTAC
ATAAAAAAAATATAAAGAATATAAAAAATATCTATTTTGTAAACCGCTACTTTGAAGGTCGCGATACCCCAATTAATCAA
AGTAATACTAGCTCTATATTCGGCTATGGCGCTCCAAGCTCAATATACAACCTAAATGAAAATGCGATAACTTTACAACT
CATACCTCAATCAAATACTTTCGAAGTAAGCCAAAGTGTTGGTGAGAAAATTAACTTTATCAACAAAATTTTTATAGCTA
GTGTAGAGCAACTAAAAACCTGTCAATTTAACGCCTATTATAAAGATAATACTCTAGTACTTAATGGCTGCTTACCATCA
AATAGTTATACTTTTAGTTTTGCTATAGAAGATCCACAACAACAGATGCAACAAGCCGTCTTAGCTGAACTAGCACAACT
TAAAATCTCGTTAGATACTCAGATAAAAACTACTAATAAACTACCGGATAATCTAACACTCTTAGCAAAACATAAATCAG
CAGATTTAACCCAACTACTAAAGCATATGCTTGTAACATCTGATAATCTCTATGCGCAAACAATTACAAGAACTATCGGT
TATTATTATAATCAAGTTGGTAGTATTGTATCTGGTAAAAATGCCATTATTAAAATACTAAAAACTAAGCTTAATCTTGA
CACTGATAGCATTCAACTTGAAGATGGTGCTGGAATGTCTGAGAATAATTTATTATCTGCGAATTTTATTGTTAGCCTGT
TACAAAAAATATCACAAAATAAGAACTTTGAGCTTTTAAAATGCCTACTGCCAATATATGGGGAAACTGGTACACTTAAA
AATCGCTCAAGCAAACTATTAAAAGGTAAAGTATTTGCAAAAATAGGAACTGAAACAACAGTTGTTGCCCTTTCTGGGTA
TCTCTATGCTAATAATAAACAATATATCTTTTCAATATTAGTTAACAATCTCAAAAACTCACAAAAATCAAGCGCTTTAG
CTTTAGAGAAAGATTTGCTTGAAAGTATATGTTAA

Upstream 100 bases:

>100_bases
ATTTTTATTAGGTTCATGTATAATTGCCATAGCACTTCTAGCTGTTTGCTGGAATCTTAGTTATAATCAAGCTTTCAGTG
ATTAATTACTTAGAATATAA

Downstream 100 bases:

>100_bases
CATTACAAAATTATAATTTAATAAAAAACTCTCAAATGACTAAAAATATTTTAATCACTGGTGCTAATGGAGGTATTGGT
TCTGCTATAGCTTTAGCAGC

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPIKLLKSLLLFFILSVFIAYPLSVNSIELNQVLDNSIEQNNLQSAQIAVKIIDSEKNTVILSKNADKNFIPASNTKLLT
GIAGLLFLGKDFRFETKLYYDKINNQSIDNLYIEFSGDPSFTREDLHKLLISLHKKNIKNIKNIYFVNRYFEGRDTPINQ
SNTSSIFGYGAPSSIYNLNENAITLQLIPQSNTFEVSQSVGEKINFINKIFIASVEQLKTCQFNAYYKDNTLVLNGCLPS
NSYTFSFAIEDPQQQMQQAVLAELAQLKISLDTQIKTTNKLPDNLTLLAKHKSADLTQLLKHMLVTSDNLYAQTITRTIG
YYYNQVGSIVSGKNAIIKILKTKLNLDTDSIQLEDGAGMSENNLLSANFIVSLLQKISQNKNFELLKCLLPIYGETGTLK
NRSSKLLKGKVFAKIGTETTVVALSGYLYANNKQYIFSILVNNLKNSQKSSALALEKDLLESIC

Sequences:

>Translated_464_residues
MPIKLLKSLLLFFILSVFIAYPLSVNSIELNQVLDNSIEQNNLQSAQIAVKIIDSEKNTVILSKNADKNFIPASNTKLLT
GIAGLLFLGKDFRFETKLYYDKINNQSIDNLYIEFSGDPSFTREDLHKLLISLHKKNIKNIKNIYFVNRYFEGRDTPINQ
SNTSSIFGYGAPSSIYNLNENAITLQLIPQSNTFEVSQSVGEKINFINKIFIASVEQLKTCQFNAYYKDNTLVLNGCLPS
NSYTFSFAIEDPQQQMQQAVLAELAQLKISLDTQIKTTNKLPDNLTLLAKHKSADLTQLLKHMLVTSDNLYAQTITRTIG
YYYNQVGSIVSGKNAIIKILKTKLNLDTDSIQLEDGAGMSENNLLSANFIVSLLQKISQNKNFELLKCLLPIYGETGTLK
NRSSKLLKGKVFAKIGTETTVVALSGYLYANNKQYIFSILVNNLKNSQKSSALALEKDLLESIC
>Mature_463_residues
PIKLLKSLLLFFILSVFIAYPLSVNSIELNQVLDNSIEQNNLQSAQIAVKIIDSEKNTVILSKNADKNFIPASNTKLLTG
IAGLLFLGKDFRFETKLYYDKINNQSIDNLYIEFSGDPSFTREDLHKLLISLHKKNIKNIKNIYFVNRYFEGRDTPINQS
NTSSIFGYGAPSSIYNLNENAITLQLIPQSNTFEVSQSVGEKINFINKIFIASVEQLKTCQFNAYYKDNTLVLNGCLPSN
SYTFSFAIEDPQQQMQQAVLAELAQLKISLDTQIKTTNKLPDNLTLLAKHKSADLTQLLKHMLVTSDNLYAQTITRTIGY
YYNQVGSIVSGKNAIIKILKTKLNLDTDSIQLEDGAGMSENNLLSANFIVSLLQKISQNKNFELLKCLLPIYGETGTLKN
RSSKLLKGKVFAKIGTETTVVALSGYLYANNKQYIFSILVNNLKNSQKSSALALEKDLLESIC

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=391, Percent_Identity=28.3887468030691, Blast_Score=135, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 51993; Mature: 51861

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIKLLKSLLLFFILSVFIAYPLSVNSIELNQVLDNSIEQNNLQSAQIAVKIIDSEKNTV
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCHHHCCCCCEEEEEEEEECCCCEE
ILSKNADKNFIPASNTKLLTGIAGLLFLGKDFRFETKLYYDKINNQSIDNLYIEFSGDPS
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHEEEEECCCCCCCCEEEEEECCCCC
FTREDLHKLLISLHKKNIKNIKNIYFVNRYFEGRDTPINQSNTSSIFGYGAPSSIYNLNE
CCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEECCCCCCEEECCC
NAITLQLIPQSNTFEVSQSVGEKINFINKIFIASVEQLKTCQFNAYYKDNTLVLNGCLPS
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEECCCC
NSYTFSFAIEDPQQQMQQAVLAELAQLKISLDTQIKTTNKLPDNLTLLAKHKSADLTQLL
CCEEEEEEECCHHHHHHHHHHHHHHHHEEEECCEEEECCCCCCCEEEEEECCCCCHHHHH
KHMLVTSDNLYAQTITRTIGYYYNQVGSIVSGKNAIIKILKTKLNLDTDSIQLEDGAGMS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCC
ENNLLSANFIVSLLQKISQNKNFELLKCLLPIYGETGTLKNRSSKLLKGKVFAKIGTETT
CCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCEEEEEECCCEE
VVALSGYLYANNKQYIFSILVNNLKNSQKSSALALEKDLLESIC
EEEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIKLLKSLLLFFILSVFIAYPLSVNSIELNQVLDNSIEQNNLQSAQIAVKIIDSEKNTV
CHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCHHHCCCCCEEEEEEEEECCCCEE
ILSKNADKNFIPASNTKLLTGIAGLLFLGKDFRFETKLYYDKINNQSIDNLYIEFSGDPS
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHEEEEECCCCCCCCEEEEEECCCCC
FTREDLHKLLISLHKKNIKNIKNIYFVNRYFEGRDTPINQSNTSSIFGYGAPSSIYNLNE
CCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEECCCCCCEEECCC
NAITLQLIPQSNTFEVSQSVGEKINFINKIFIASVEQLKTCQFNAYYKDNTLVLNGCLPS
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEECCCC
NSYTFSFAIEDPQQQMQQAVLAELAQLKISLDTQIKTTNKLPDNLTLLAKHKSADLTQLL
CCEEEEEEECCHHHHHHHHHHHHHHHHEEEECCEEEECCCCCCCEEEEEECCCCCHHHHH
KHMLVTSDNLYAQTITRTIGYYYNQVGSIVSGKNAIIKILKTKLNLDTDSIQLEDGAGMS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCC
ENNLLSANFIVSLLQKISQNKNFELLKCLLPIYGETGTLKNRSSKLLKGKVFAKIGTETT
CCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCEEEEEECCCEE
VVALSGYLYANNKQYIFSILVNNLKNSQKSSALALEKDLLESIC
EEEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]