BacMap

Definition	Francisella tularensis subsp. tularensis WY96-3418, complete genome.
Accession	NC_009257
Length	1,898,476

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The map label for this gene is ygfF [H]

Identifier: 134302387

GI number: 134302387

Start: 1421446

End: 1422234

Strand: Direct

Name: ygfF [H]

Synonym: FTW_1515

Alternate gene names: 134302387

Gene position: 1421446-1422234 (Clockwise)

Preceding gene: 134302386

Following gene: 134302388

Centisome position: 74.87

GC content: 31.94

Gene sequence:

>789_bases
ATGTTAACATTACAAAATTATAATTTAATAAAAAACTCTCAAATGACTAAAAATATTTTAATCACTGGTGCTAATGGAGG
TATTGGTTCTGCTATAGCTTTAGCAGCAGCAGAAGCTGGATATAATATAGGTCTTCATTATCATATTAATAATCAAAATA
TTGATAAAATAAAGAAACAACTTGCTCAATATCCCATTCAAATTTCAACTCATCGTGCTGATATAGCAAATGAAGAAGAT
GTTAGAGAAATGTTTAATGAATTTATTTCTTATCACGGTTCTATGTATGCCCTTATAAATAATGCAAGTACAATTTTTCA
TACCAGCCGTTTAGAAGATATGAGTATTGATCGTCTACAGAAAGTATTCCAAGTGAATGCTATAGGAAATATTCTATGTG
CCAAACACGCAATAAAAAAGATGTCCAGAAAATATGGATACCATGGTGGTAAAATTATTAATATATCCTCTAGTGCATCT
CGCTCTGGCTCTCCTAATGAATACATAGATTATGCTTGTACTAAAGGTGCTATTGATACTCTTACAAAAGGCTTATCAAA
AGAACTTGCTGATGAAGGCATTCTTGTAAATTGTATAAGACCTGGATCTACATATACAGATATTCATAAACTATCAGGTG
ATGCTGATAGAGTTAATAAAATAAAACACAAAATCCCACTAAAAAGAGGTGGTTACCCTGAAGAAATCGCCAGTGGAGTA
ATATGGCTACTATCTGATGGTGCAAACTATTGCACTGGGACTTTTATAGATATCGCTGGTGGTAAATAA

Upstream 100 bases:

>100_bases
CTAATAATAAACAATATATCTTTTCAATATTAGTTAACAATCTCAAAAACTCACAAAAATCAAGCGCTTTAGCTTTAGAG
AAAGATTTGCTTGAAAGTAT

Downstream 100 bases:

>100_bases
AGTTAGGAATATTAATTTCACATAATAATACTTTATATGCCAAAGTTATGAGTTCTACCAATATACAACAACTGTTATTT
GTGCTATATGATACAAAGAT

Product: oxidoreductase, short-chain dehydrogenase/reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MLTLQNYNLIKNSQMTKNILITGANGGIGSAIALAAAEAGYNIGLHYHINNQNIDKIKKQLAQYPIQISTHRADIANEED
VREMFNEFISYHGSMYALINNASTIFHTSRLEDMSIDRLQKVFQVNAIGNILCAKHAIKKMSRKYGYHGGKIINISSSAS
RSGSPNEYIDYACTKGAIDTLTKGLSKELADEGILVNCIRPGSTYTDIHKLSGDADRVNKIKHKIPLKRGGYPEEIASGV
IWLLSDGANYCTGTFIDIAGGK

Sequences:

>Translated_262_residues
MLTLQNYNLIKNSQMTKNILITGANGGIGSAIALAAAEAGYNIGLHYHINNQNIDKIKKQLAQYPIQISTHRADIANEED
VREMFNEFISYHGSMYALINNASTIFHTSRLEDMSIDRLQKVFQVNAIGNILCAKHAIKKMSRKYGYHGGKIINISSSAS
RSGSPNEYIDYACTKGAIDTLTKGLSKELADEGILVNCIRPGSTYTDIHKLSGDADRVNKIKHKIPLKRGGYPEEIASGV
IWLLSDGANYCTGTFIDIAGGK
>Mature_262_residues
MLTLQNYNLIKNSQMTKNILITGANGGIGSAIALAAAEAGYNIGLHYHINNQNIDKIKKQLAQYPIQISTHRADIANEED
VREMFNEFISYHGSMYALINNASTIFHTSRLEDMSIDRLQKVFQVNAIGNILCAKHAIKKMSRKYGYHGGKIINISSSAS
RSGSPNEYIDYACTKGAIDTLTKGLSKELADEGILVNCIRPGSTYTDIHKLSGDADRVNKIKHKIPLKRGGYPEEIASGV
IWLLSDGANYCTGTFIDIAGGK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=246, Percent_Identity=28.4552845528455, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI59889578, Length=193, Percent_Identity=30.0518134715026, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI109715829, Length=234, Percent_Identity=31.6239316239316, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI19923817, Length=259, Percent_Identity=29.3436293436293, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI40254992, Length=251, Percent_Identity=28.2868525896414, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI15277342, Length=258, Percent_Identity=26.3565891472868, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI7705925, Length=247, Percent_Identity=24.6963562753036, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI126723750, Length=264, Percent_Identity=23.1060606060606, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI2367175, Length=243, Percent_Identity=44.8559670781893, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1788459, Length=247, Percent_Identity=31.9838056680162, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789378, Length=248, Percent_Identity=27.0161290322581, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786812, Length=255, Percent_Identity=30.1960784313725, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787905, Length=245, Percent_Identity=27.3469387755102, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790717, Length=246, Percent_Identity=27.6422764227642, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789208, Length=246, Percent_Identity=28.0487804878049, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787335, Length=243, Percent_Identity=30.4526748971193, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI2367365, Length=245, Percent_Identity=26.1224489795918, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082160, Length=249, Percent_Identity=27.710843373494, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=32.6771653543307, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789057, Length=201, Percent_Identity=27.8606965174129, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=248, Percent_Identity=31.8548387096774, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71994604, Length=256, Percent_Identity=26.953125, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=257, Percent_Identity=27.6264591439689, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17555706, Length=265, Percent_Identity=27.9245283018868, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=249, Percent_Identity=26.5060240963855, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=242, Percent_Identity=29.7520661157025, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560150, Length=243, Percent_Identity=26.3374485596708, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=243, Percent_Identity=27.9835390946502, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=250, Percent_Identity=28.4, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=245, Percent_Identity=25.3061224489796, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=267, Percent_Identity=25.4681647940075, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17563726, Length=242, Percent_Identity=25.2066115702479, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=247, Percent_Identity=25.9109311740891, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536025, Length=263, Percent_Identity=27.3764258555133, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=264, Percent_Identity=26.8939393939394, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=261, Percent_Identity=25.2873563218391, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17538480, Length=265, Percent_Identity=27.5471698113208, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=261, Percent_Identity=25.6704980842912, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=262, Percent_Identity=24.8091603053435, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=272, Percent_Identity=28.3088235294118, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=265, Percent_Identity=25.6603773584906, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17561272, Length=181, Percent_Identity=29.8342541436464, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=226, Percent_Identity=25.6637168141593, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=190, Percent_Identity=27.8947368421053, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323882, Length=197, Percent_Identity=28.4263959390863, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24644339, Length=255, Percent_Identity=30.5882352941176, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=28.125, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI23397609, Length=251, Percent_Identity=29.8804780876494, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355319, Length=247, Percent_Identity=31.1740890688259, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357041, Length=260, Percent_Identity=28.0769230769231, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24641232, Length=238, Percent_Identity=28.9915966386555, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24639444, Length=251, Percent_Identity=27.4900398406374, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24643142, Length=246, Percent_Identity=27.2357723577236, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116007236, Length=240, Percent_Identity=25.4166666666667, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 28713; Mature: 28713

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLQNYNLIKNSQMTKNILITGANGGIGSAIALAAAEAGYNIGLHYHINNQNIDKIKKQ
CEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHHH
LAQYPIQISTHRADIANEEDVREMFNEFISYHGSMYALINNASTIFHTSRLEDMSIDRLQ
HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCHHHHH
KVFQVNAIGNILCAKHAIKKMSRKYGYHGGKIINISSSASRSGSPNEYIDYACTKGAIDT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCHHHH
LTKGLSKELADEGILVNCIRPGSTYTDIHKLSGDADRVNKIKHKIPLKRGGYPEEIASGV
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHCCE
IWLLSDGANYCTGTFIDIAGGK
EEEEECCCCCCCEEEEEECCCC
>Mature Secondary Structure
MLTLQNYNLIKNSQMTKNILITGANGGIGSAIALAAAEAGYNIGLHYHINNQNIDKIKKQ
CEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHHH
LAQYPIQISTHRADIANEEDVREMFNEFISYHGSMYALINNASTIFHTSRLEDMSIDRLQ
HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCHHHHH
KVFQVNAIGNILCAKHAIKKMSRKYGYHGGKIINISSSASRSGSPNEYIDYACTKGAIDT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCHHHH
LTKGLSKELADEGILVNCIRPGSTYTDIHKLSGDADRVNKIKHKIPLKRGGYPEEIASGV
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHCCE
IWLLSDGANYCTGTFIDIAGGK
EEEEECCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 7764507 [H]