Click here to switch to the map view.

The map label for this gene is phrB2 [H]

Identifier: 134302253

GI number: 134302253

Start: 1260609

End: 1262108

Strand: Reverse

Name: phrB2 [H]

Synonym: FTW_1339

Alternate gene names: 134302253

Gene position: 1262108-1260609 (Counterclockwise)

Preceding gene: 134302254

Following gene: 134302252

Centisome position: 66.48

GC content: 34.13

Gene sequence:

>1500_bases
ATGCAGATAGTTTGGTTTAAGCGAGACTTACGTGTAACTGATAACTTAGCTTTATCATTAGCCTCTGAAAAAGGAGATAT
TTTACCACTATACATAATTGAACTTGAACTTTGGCAACAGCCTGATATGAGTCATAGGCAATATTTGTTTTTATCAGAGT
GTCTAGAAGAGCTAAATACTGAGCTTACAAAATTGGGTCAATCTTTAGCTATAATGCTAGGGGATGCTGTAGAGATTTTT
GAGCAGCTAATACAAAAATATAATATAAAGAATGTTTGGTCGCATCAAGAAACTTGGAATGACTGGACATATCAGCGAGA
TATTAAACTTGAGAAATTTTTTAAACAGAATAATATTGTATGGCATCAACCATATCAAAATGGTGTAGTTCGTTGTTTAG
CTGATAGAGATAACTGGGCTTTGTTATGGCATCAGCGCATGAGTGAAAAGATTATAAGAGCGCCTACCAAACTTAAGTTT
ATTTGCGAAAACCAAATCAAAATACCTACTGCTGAGAGCCTTGGTCTAGAGTATGATGATTGTTACAAAAGACAAAAAGG
CGGTCGAATTCGTGCACTTAGAATTCTTGATAGTTTTTTATATCAAAGGGGGTGCGGCTATACCAAAGAAATGTCTTCAC
CTGTAACAGCTTTTAAAAGTTGTTCAAGGTTATCGCCATATATAGCTTTTGGAGTTATTTCGTTAAAAGAGATATACCAG
AAAGCTAATCAGCGTAAAAATGAAATCAAAGAAAGTAGCGTAAAAAATAAAACAAAATGGTTAAGTGCAATGCGTTCATT
TTTATCACGTTTGCGTTGGCATTGTCATTTTATGCAGAAGTTAGAGGATCAACCAAGTATTGAATATGAGAATTTGCATT
CAGCTTATGATCAACTACGTACAGAACCGCTAAATCAACAATGTTTTGAAGCATGGAAAACTGGCAATACTGGTTATCCA
ATGATAGATGCTTGTATGCGAGCTTTGATTGCGACAGGGTGGCTAAATTTTCGTATGCGTGCAATGCTTATGAGTTTTGC
AAGCTATCATTTGTGGTTAGATTGGCGTGTTACATCGCTATATTTAGCAAGATTATTTACTGATTATGAGCCAGGTATTC
ATTACTCACAAGTACAAATGCAATCTGGGACTACAGGTATTAATAGTATCCGTATTTATAACCCTATTAAGCAAAGCATT
GACCAAGATCCTAATGGCGAGTTTATTCGAAGATGGCTACCTGAACTAGAAAATGTAAGTAATGAAAATATTCATACTCC
ATGGTTAGAAAAACATAATTCTCTAGATTATCCAGATCCAACCATTGATGAAAAACAAGCACGTAAATTTGCTGCAGATA
ATATTTATAAAATTAGAAAAAGCTCTAAAAATAGTCAAGAAACTAAAAATATTGTCAAAAAACACGCCAGTAGGAAAACG
TCTAGAAAAATAAAATCTAAACAACACAAGGTAGAGAGCATACAAGGAGAATTATTCTAA

Upstream 100 bases:

>100_bases
AAGATTATGCGCAACAATCTTAAGCCGAGTAGTTTTAAGCTTTGGCGTAAAAGAGTAACAGGAAGAGTTGCAAAACATCA
AAAAAGGTTAAATCATATAA

Downstream 100 bases:

>100_bases
TGAGGACTTTACGATTAATTCTAGGAGATCAGCTTTCACAGAGTATTTCTAGCTTGAGAGATTGTGATAAAGCAAATGAT
GTTGTGATGATGTGTGAAGT

Product: deoxyribodipyrimidine photolyase

Products: 2 pyrimidine residues (in DNA) [C]

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MQIVWFKRDLRVTDNLALSLASEKGDILPLYIIELELWQQPDMSHRQYLFLSECLEELNTELTKLGQSLAIMLGDAVEIF
EQLIQKYNIKNVWSHQETWNDWTYQRDIKLEKFFKQNNIVWHQPYQNGVVRCLADRDNWALLWHQRMSEKIIRAPTKLKF
ICENQIKIPTAESLGLEYDDCYKRQKGGRIRALRILDSFLYQRGCGYTKEMSSPVTAFKSCSRLSPYIAFGVISLKEIYQ
KANQRKNEIKESSVKNKTKWLSAMRSFLSRLRWHCHFMQKLEDQPSIEYENLHSAYDQLRTEPLNQQCFEAWKTGNTGYP
MIDACMRALIATGWLNFRMRAMLMSFASYHLWLDWRVTSLYLARLFTDYEPGIHYSQVQMQSGTTGINSIRIYNPIKQSI
DQDPNGEFIRRWLPELENVSNENIHTPWLEKHNSLDYPDPTIDEKQARKFAADNIYKIRKSSKNSQETKNIVKKHASRKT
SRKIKSKQHKVESIQGELF

Sequences:

>Translated_499_residues
MQIVWFKRDLRVTDNLALSLASEKGDILPLYIIELELWQQPDMSHRQYLFLSECLEELNTELTKLGQSLAIMLGDAVEIF
EQLIQKYNIKNVWSHQETWNDWTYQRDIKLEKFFKQNNIVWHQPYQNGVVRCLADRDNWALLWHQRMSEKIIRAPTKLKF
ICENQIKIPTAESLGLEYDDCYKRQKGGRIRALRILDSFLYQRGCGYTKEMSSPVTAFKSCSRLSPYIAFGVISLKEIYQ
KANQRKNEIKESSVKNKTKWLSAMRSFLSRLRWHCHFMQKLEDQPSIEYENLHSAYDQLRTEPLNQQCFEAWKTGNTGYP
MIDACMRALIATGWLNFRMRAMLMSFASYHLWLDWRVTSLYLARLFTDYEPGIHYSQVQMQSGTTGINSIRIYNPIKQSI
DQDPNGEFIRRWLPELENVSNENIHTPWLEKHNSLDYPDPTIDEKQARKFAADNIYKIRKSSKNSQETKNIVKKHASRKT
SRKIKSKQHKVESIQGELF
>Mature_499_residues
MQIVWFKRDLRVTDNLALSLASEKGDILPLYIIELELWQQPDMSHRQYLFLSECLEELNTELTKLGQSLAIMLGDAVEIF
EQLIQKYNIKNVWSHQETWNDWTYQRDIKLEKFFKQNNIVWHQPYQNGVVRCLADRDNWALLWHQRMSEKIIRAPTKLKF
ICENQIKIPTAESLGLEYDDCYKRQKGGRIRALRILDSFLYQRGCGYTKEMSSPVTAFKSCSRLSPYIAFGVISLKEIYQ
KANQRKNEIKESSVKNKTKWLSAMRSFLSRLRWHCHFMQKLEDQPSIEYENLHSAYDQLRTEPLNQQCFEAWKTGNTGYP
MIDACMRALIATGWLNFRMRAMLMSFASYHLWLDWRVTSLYLARLFTDYEPGIHYSQVQMQSGTTGINSIRIYNPIKQSI
DQDPNGEFIRRWLPELENVSNENIHTPWLEKHNSLDYPDPTIDEKQARKFAADNIYKIRKSSKNSQETKNIVKKHASRKT
SRKIKSKQHKVESIQGELF

Specific function: Has no photolyase activity [H]

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=500, Percent_Identity=23, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI188536100, Length=501, Percent_Identity=22.3552894211577, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI188536103, Length=452, Percent_Identity=21.9026548672566, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786926, Length=475, Percent_Identity=26.5263157894737, Blast_Score=139, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324962, Length=268, Percent_Identity=30.2238805970149, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137248, Length=527, Percent_Identity=22.7703984819734, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24585455, Length=527, Percent_Identity=22.7703984819734, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24648152, Length=500, Percent_Identity=21.4, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: 4.1.99.3 [C]

Molecular weight: Translated: 58997; Mature: 58997

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIVWFKRDLRVTDNLALSLASEKGDILPLYIIELELWQQPDMSHRQYLFLSECLEELNT
CEEEEEECCCCCCCHHHEEEECCCCCEEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHH
ELTKLGQSLAIMLGDAVEIFEQLIQKYNIKNVWSHQETWNDWTYQRDIKLEKFFKQNNIV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEECCCHHHHHHHHCCCE
WHQPYQNGVVRCLADRDNWALLWHQRMSEKIIRAPTKLKFICENQIKIPTAESLGLEYDD
EECCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHCCCCHHH
CYKRQKGGRIRALRILDSFLYQRGCGYTKEMSSPVTAFKSCSRLSPYIAFGVISLKEIYQ
HHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
KANQRKNEIKESSVKNKTKWLSAMRSFLSRLRWHCHFMQKLEDQPSIEYENLHSAYDQLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TEPLNQQCFEAWKTGNTGYPMIDACMRALIATGWLNFRMRAMLMSFASYHLWLDWRVTSL
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHH
YLARLFTDYEPGIHYSQVQMQSGTTGINSIRIYNPIKQSIDQDPNGEFIRRWLPELENVS
HHHHHHHCCCCCCCHHHEEECCCCCCCCCEEEECHHHHHCCCCCCHHHHHHHHHHHHCCC
NENIHTPWLEKHNSLDYPDPTIDEKQARKFAADNIYKIRKSSKNSQETKNIVKKHASRKT
CCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SRKIKSKQHKVESIQGELF
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQIVWFKRDLRVTDNLALSLASEKGDILPLYIIELELWQQPDMSHRQYLFLSECLEELNT
CEEEEEECCCCCCCHHHEEEECCCCCEEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHH
ELTKLGQSLAIMLGDAVEIFEQLIQKYNIKNVWSHQETWNDWTYQRDIKLEKFFKQNNIV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEECCCHHHHHHHHCCCE
WHQPYQNGVVRCLADRDNWALLWHQRMSEKIIRAPTKLKFICENQIKIPTAESLGLEYDD
EECCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHCCCCHHH
CYKRQKGGRIRALRILDSFLYQRGCGYTKEMSSPVTAFKSCSRLSPYIAFGVISLKEIYQ
HHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
KANQRKNEIKESSVKNKTKWLSAMRSFLSRLRWHCHFMQKLEDQPSIEYENLHSAYDQLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TEPLNQQCFEAWKTGNTGYPMIDACMRALIATGWLNFRMRAMLMSFASYHLWLDWRVTSL
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHH
YLARLFTDYEPGIHYSQVQMQSGTTGINSIRIYNPIKQSIDQDPNGEFIRRWLPELENVS
HHHHHHHCCCCCCCHHHEEECCCCCCCCCEEEECHHHHHCCCCCCHHHHHHHHHHHHCCC
NENIHTPWLEKHNSLDYPDPTIDEKQARKFAADNIYKIRKSSKNSQETKNIVKKHASRKT
CCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SRKIKSKQHKVESIQGELF
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; FADH2; Flavin; pterin [C]

Metal ions: NA

Kcat value (1/min): 2.4 [C]

Specific activity: NA

Km value (mM): NA

Substrates: cyclobutadipyrimidine (in DNA) [C]

Specific reaction: cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) [C]

General reaction: C-C-bond cleavage [C]

Inhibitor: yeast DNA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]