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The map label for this gene is rrmJ

Identifier: 134302197

GI number: 134302197

Start: 1200781

End: 1201401

Strand: Direct

Name: rrmJ

Synonym: FTW_1267

Alternate gene names: 134302197

Gene position: 1200781-1201401 (Clockwise)

Preceding gene: 134302195

Following gene: 134302203

Centisome position: 63.25

GC content: 33.17

Gene sequence:

>621_bases
ATGTCAAAAGGTTCTAGTACTAAAAAATGGTTACATGAGCATACTTCGGATTACTATGTTATCCAAGCAAATAAGCTTGG
CTATCGTAGTAGAGCAAGCTTTAAAATTCTTGAAATACAAGATAAATATCAATTATTTAAACCAAATATGTTTGTTGTCG
ATCTTGGTGCAGCTCCAGGTGGTTGGTCTGAGCAAGTTATAAAATATATAGGTAAAAATGGTAAGCTTATTGCTCTAGAT
CTACTAGAAATGGCTCCAATAGCTGGAGTAGAGTTTATTCAAGGCGACTTCTCTAGTGATGAAACTTACCAAAAACTCAA
TACTCTTGTTAATAATCAAAAGATTGATTGTGTTATCTCTGATATGGCGCCAAATCTAAGTGGTAACAAAACTTCAGATC
AAGCAAAATCGATATATTTGCTTGAATTAGCTTTGGATTTTGCTAATACTAATCTCAACAAAAACGGCAGTTTCGTTGCA
AAAGTCTTTCAAGGTCAAGGCAGTGATGAATATCTCAAATTAGTAAGAGAGTCTTTTAATAAAGTGATTCAATTTAAACC
AAAATCTTCAAGAGCAAAGTCACGAGAGTTTTATGTTATCGCAACTGAATTTAAGGGTTGA

Upstream 100 bases:

>100_bases
TATTTTATAAGCTTTTATAATATACAATTCTAATAACTCCAAATCTTTATTTATTATAAAAATATCCATACAATATACAA
AAAAATAATTAGCTATATAA

Downstream 100 bases:

>100_bases
CTTATCCTTCAAACACATAATTTATAAATAGAATTTTTAAGATTAGTCTATTTTATAGTTTATTTAAAAAAGTCTCCGCT
AAAATTTTCTTCTAACTTTT

Product: ribosomal large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSKGSSTKKWLHEHTSDYYVIQANKLGYRSRASFKILEIQDKYQLFKPNMFVVDLGAAPGGWSEQVIKYIGKNGKLIALD
LLEMAPIAGVEFIQGDFSSDETYQKLNTLVNNQKIDCVISDMAPNLSGNKTSDQAKSIYLLELALDFANTNLNKNGSFVA
KVFQGQGSDEYLKLVRESFNKVIQFKPKSSRAKSREFYVIATEFKG

Sequences:

>Translated_206_residues
MSKGSSTKKWLHEHTSDYYVIQANKLGYRSRASFKILEIQDKYQLFKPNMFVVDLGAAPGGWSEQVIKYIGKNGKLIALD
LLEMAPIAGVEFIQGDFSSDETYQKLNTLVNNQKIDCVISDMAPNLSGNKTSDQAKSIYLLELALDFANTNLNKNGSFVA
KVFQGQGSDEYLKLVRESFNKVIQFKPKSSRAKSREFYVIATEFKG
>Mature_205_residues
SKGSSTKKWLHEHTSDYYVIQANKLGYRSRASFKILEIQDKYQLFKPNMFVVDLGAAPGGWSEQVIKYIGKNGKLIALDL
LEMAPIAGVEFIQGDFSSDETYQKLNTLVNNQKIDCVISDMAPNLSGNKTSDQAKSIYLLELALDFANTNLNKNGSFVAK
VFQGQGSDEYLKLVRESFNKVIQFKPKSSRAKSREFYVIATEFKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=206, Percent_Identity=31.5533980582524, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI29029591, Length=187, Percent_Identity=35.2941176470588, Blast_Score=114, Evalue=5e-26,
Organism=Homo sapiens, GI29029589, Length=187, Percent_Identity=35.2941176470588, Blast_Score=114, Evalue=5e-26,
Organism=Homo sapiens, GI7110661, Length=187, Percent_Identity=35.2941176470588, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=30.3191489361702, Blast_Score=110, Evalue=7e-25,
Organism=Escherichia coli, GI1789569, Length=200, Percent_Identity=54, Blast_Score=223, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI17553474, Length=202, Percent_Identity=30.1980198019802, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17554650, Length=207, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71987550, Length=185, Percent_Identity=31.8918918918919, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71987561, Length=188, Percent_Identity=33.5106382978723, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17553860, Length=188, Percent_Identity=33.5106382978723, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319535, Length=195, Percent_Identity=33.8461538461538, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=34.5549738219895, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321302, Length=280, Percent_Identity=27.5, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356387, Length=213, Percent_Identity=32.3943661971831, Blast_Score=130, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=33.1632653061224, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18859957, Length=179, Percent_Identity=32.4022346368715, Blast_Score=100, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_FRAT1 (Q14HT5)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_667041.1
- ProteinModelPortal:   Q14HT5
- SMR:   Q14HT5
- STRING:   Q14HT5
- GeneID:   4198962
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF0912c
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q14HT5
- ProtClustDB:   CLSK934750
- BioCyc:   FTUL393115:FTF0912C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23160; Mature: 23028

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: ACT_SITE 161-161 BINDING 60-60 BINDING 62-62 BINDING 80-80 BINDING 96-96 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGSSTKKWLHEHTSDYYVIQANKLGYRSRASFKILEIQDKYQLFKPNMFVVDLGAAPG
CCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCHHEECCCEEEEECCCCCC
GWSEQVIKYIGKNGKLIALDLLEMAPIAGVEFIQGDFSSDETYQKLNTLVNNQKIDCVIS
CHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCEECCCCCCCHHHHHHHHHHCCCEEEEEEE
DMAPNLSGNKTSDQAKSIYLLELALDFANTNLNKNGSFVAKVFQGQGSDEYLKLVRESFN
CCCCCCCCCCCCCHHHEEEEEEEEEHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
KVIQFKPKSSRAKSREFYVIATEFKG
HHEEECCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
SKGSSTKKWLHEHTSDYYVIQANKLGYRSRASFKILEIQDKYQLFKPNMFVVDLGAAPG
CCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCHHEECCCEEEEECCCCCC
GWSEQVIKYIGKNGKLIALDLLEMAPIAGVEFIQGDFSSDETYQKLNTLVNNQKIDCVIS
CHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCEECCCCCCCHHHHHHHHHHCCCEEEEEEE
DMAPNLSGNKTSDQAKSIYLLELALDFANTNLNKNGSFVAKVFQGQGSDEYLKLVRESFN
CCCCCCCCCCCCCHHHEEEEEEEEEHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
KVIQFKPKSSRAKSREFYVIATEFKG
HHEEECCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA