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The map label for this gene is topA [H]

Identifier: 134302203

GI number: 134302203

Start: 1205400

End: 1207694

Strand: Direct

Name: topA [H]

Synonym: FTW_1273

Alternate gene names: 134302203

Gene position: 1205400-1207694 (Clockwise)

Preceding gene: 134302197

Following gene: 134302210

Centisome position: 63.49

GC content: 34.55

Gene sequence:

>2295_bases
ATGGCAAAAAATTTAGTAATCGTAGAGTCTCCAGCCAAAACTAAGACTATAAAAAAATACCTTGGTAACGAATTTGAAAT
ATTAGCATCATTTGGACACGTACGCGAAATACCTTCCAAAGATACTTCTATCGATGTTAATGATAATTTTAAGATTAAGT
TCACAACTAGTGAAAAAAGCAAAAAACATTTAGATGCAATCAAAAAAGCAGCTAAAGATGCTCAAAATATCTATCTCGCT
ACTGACCCAGATAGAGAAGGTGAAGCTATATCATGGCATGTACAAGAAGTATTAAAAAGTGCACGCTTACTTAAAGATAA
AAATATCTATCGTGTCACATTTAACGAAATCACAAAATCAGCAGTTACAAATGCTATAGCAAATCCAAAAGAGTTATCTA
TGGATTTAGTTGATGCTCAAAAAGCACGCCAAGCACTAGATTTTCTGGTTGGCTTTAATATATCTCCGCTATTATGGCGT
AAAATCACCAGTGGCTTATCAGCAGGAAGAGTACAAAGTCCTGCTCTAAGAATGATTGTCGAGCGTGAAATAGAGCGTGA
AAACTTCATCAAACAAGATTATTGGAGTCTAACTGCAGATACTTTCAAACAAAAACAAATATTTGCAAATTTGCTTGAAT
TTAATAATAATAAAGTTGAACAATTCACTTTCACAACAGCAGAAGATGCTGAAAATGCAAAAGCCCATATCCTAAAAGAT
GCTAATGGTTTTTTGATAGTTGATGGCATTACTGAGAAAAAAACACGGAGAAACCCATATCCTCCATTTATAACATCAAC
ACTACAGCAAGAGGCTTCAAAAAAACTCGGCTTTACCGCTAAAAGAACAATGTCTACTGCACAAAAATTATATGAAGGTA
TTGATCTAGGCAATGGTGAATCCGTTGGTCTTATCTCGTACATGAGAACAGATTCAACCAACCTTTCTAACGATGCTCTT
AATGATATTAGAAACTTTATTCAAGCAAAATATGATAAGGATATGCTACCAGCTAAGCCACGAGTTTTTGAGAAAAAATC
ACAGAATGCACAAGAAGCACACGAAGCTATCCGTGTCACAGCTGCGAATAGAACTCCAGAATCAATAAAGCAGTTTTTAA
CTAATGATGAATTTAAGCTCTATAGCCTAATCTACAATCGAACTATTGCTTGTCAAATGAAACATGCAACTTTAAATAGT
ACATCAGTAGATTTAATTACCGAAAATACTAAACATAGGTTTAGAGTTACAGGAACTGTTATAGTAGATGCCGGATTCTT
GAAAATTTATAATGTTGAGAAAGATGAGGATGAAAAAGACTCAGATGATGAGCAAACTTTACCAAAATTTGAAAAAGGTG
AAAAAATAATACTAAATGATGTAATTACTAAAGCTCACTCAACAGAGCCACCACCTCGCTATACCGAAGCATCTTTGGTT
AAAGCATTAGAAAAATATGGTATTGGTAGACCTTCAACCTATGCAACAATCATCTCAACTTTACAGCAACGTGAGTATGT
TGAGGTAGACAAACGTCGTTTTATCCCTACAGATAAAGGTCGTATCGTAAATAAATTTCTAACTGAATATTTCAAAAAAT
ATGTTGAGTACTCTTATACCGCAGGCTTAGAAAAAGAGCTTGATGAAATTGCTCATCACAAAAATGATTACCTAAGTGTA
TTAAATAATTTCTGGCATCCATTTATTGAGAGAATCAATAAGATTTCTGAAGATGTCTCACGCAAAGATGTTGTGCAAGA
AGAGCTAGAGGAAGACTGTCCTGAATGCGGTAGTAAATTATCACTTCGACTTGGTAAAAATGGTAGATTTATTGGCTGTA
CAAACTACCCAACTTGTAAATACACCCGTCCAGTAGATAGTGATCCTAAAGAAGTCATCAAAGAAGAACCTATAGTCGTA
GAAGATAGAAAATGCCCTAAGTGCAATTCTGATTTGCATATCAAACAAGGTCGCTATGGTAAGTTTATTGGTTGCTCAAA
TTATCCTGAATGCAAACATATGGAACCTCTTGAAAAACCCAAAGATACTGGTGCAGTTTGTCCTAAATGTAATAAAAATC
ATATTGTTGAGAAAAAATCTCGCAAAGGTAAAGTTTTCTATGCTTGTGCTGGCTTCCCTAAGTGTAAAAATGCTTATTGG
TATCCACCAATAAAAGAAGAGTGTCCTAAATGCCACTCACCAATACTGTTACACAAGATAACCAAAAAAGATGGCGAACA
GAAAGCTTGCCCTAATAGCGAGTGTGACTATGCTGTAGCATTTGAAACTAAATAA

Upstream 100 bases:

>100_bases
TAACTATTGATAAGCTGCTTGTTTTCTTATATTTTATTAAATCACCGCTGAGTATTCACATAAATACTCTTTTTATAAAA
AATCATTTCAAAGGTTAAAA

Downstream 100 bases:

>100_bases
ATTATATCCTTTCAAGCTTAGATTTATAGAGTATTCTGTATGTTTAAAATAAATTATATCCACAAAGGTGGAAATATAAG
TGGTAAAATTGCAATACAAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 764; Mature: 763

Protein sequence:

>764_residues
MAKNLVIVESPAKTKTIKKYLGNEFEILASFGHVREIPSKDTSIDVNDNFKIKFTTSEKSKKHLDAIKKAAKDAQNIYLA
TDPDREGEAISWHVQEVLKSARLLKDKNIYRVTFNEITKSAVTNAIANPKELSMDLVDAQKARQALDFLVGFNISPLLWR
KITSGLSAGRVQSPALRMIVEREIERENFIKQDYWSLTADTFKQKQIFANLLEFNNNKVEQFTFTTAEDAENAKAHILKD
ANGFLIVDGITEKKTRRNPYPPFITSTLQQEASKKLGFTAKRTMSTAQKLYEGIDLGNGESVGLISYMRTDSTNLSNDAL
NDIRNFIQAKYDKDMLPAKPRVFEKKSQNAQEAHEAIRVTAANRTPESIKQFLTNDEFKLYSLIYNRTIACQMKHATLNS
TSVDLITENTKHRFRVTGTVIVDAGFLKIYNVEKDEDEKDSDDEQTLPKFEKGEKIILNDVITKAHSTEPPPRYTEASLV
KALEKYGIGRPSTYATIISTLQQREYVEVDKRRFIPTDKGRIVNKFLTEYFKKYVEYSYTAGLEKELDEIAHHKNDYLSV
LNNFWHPFIERINKISEDVSRKDVVQEELEEDCPECGSKLSLRLGKNGRFIGCTNYPTCKYTRPVDSDPKEVIKEEPIVV
EDRKCPKCNSDLHIKQGRYGKFIGCSNYPECKHMEPLEKPKDTGAVCPKCNKNHIVEKKSRKGKVFYACAGFPKCKNAYW
YPPIKEECPKCHSPILLHKITKKDGEQKACPNSECDYAVAFETK

Sequences:

>Translated_764_residues
MAKNLVIVESPAKTKTIKKYLGNEFEILASFGHVREIPSKDTSIDVNDNFKIKFTTSEKSKKHLDAIKKAAKDAQNIYLA
TDPDREGEAISWHVQEVLKSARLLKDKNIYRVTFNEITKSAVTNAIANPKELSMDLVDAQKARQALDFLVGFNISPLLWR
KITSGLSAGRVQSPALRMIVEREIERENFIKQDYWSLTADTFKQKQIFANLLEFNNNKVEQFTFTTAEDAENAKAHILKD
ANGFLIVDGITEKKTRRNPYPPFITSTLQQEASKKLGFTAKRTMSTAQKLYEGIDLGNGESVGLISYMRTDSTNLSNDAL
NDIRNFIQAKYDKDMLPAKPRVFEKKSQNAQEAHEAIRVTAANRTPESIKQFLTNDEFKLYSLIYNRTIACQMKHATLNS
TSVDLITENTKHRFRVTGTVIVDAGFLKIYNVEKDEDEKDSDDEQTLPKFEKGEKIILNDVITKAHSTEPPPRYTEASLV
KALEKYGIGRPSTYATIISTLQQREYVEVDKRRFIPTDKGRIVNKFLTEYFKKYVEYSYTAGLEKELDEIAHHKNDYLSV
LNNFWHPFIERINKISEDVSRKDVVQEELEEDCPECGSKLSLRLGKNGRFIGCTNYPTCKYTRPVDSDPKEVIKEEPIVV
EDRKCPKCNSDLHIKQGRYGKFIGCSNYPECKHMEPLEKPKDTGAVCPKCNKNHIVEKKSRKGKVFYACAGFPKCKNAYW
YPPIKEECPKCHSPILLHKITKKDGEQKACPNSECDYAVAFETK
>Mature_763_residues
AKNLVIVESPAKTKTIKKYLGNEFEILASFGHVREIPSKDTSIDVNDNFKIKFTTSEKSKKHLDAIKKAAKDAQNIYLAT
DPDREGEAISWHVQEVLKSARLLKDKNIYRVTFNEITKSAVTNAIANPKELSMDLVDAQKARQALDFLVGFNISPLLWRK
ITSGLSAGRVQSPALRMIVEREIERENFIKQDYWSLTADTFKQKQIFANLLEFNNNKVEQFTFTTAEDAENAKAHILKDA
NGFLIVDGITEKKTRRNPYPPFITSTLQQEASKKLGFTAKRTMSTAQKLYEGIDLGNGESVGLISYMRTDSTNLSNDALN
DIRNFIQAKYDKDMLPAKPRVFEKKSQNAQEAHEAIRVTAANRTPESIKQFLTNDEFKLYSLIYNRTIACQMKHATLNST
SVDLITENTKHRFRVTGTVIVDAGFLKIYNVEKDEDEKDSDDEQTLPKFEKGEKIILNDVITKAHSTEPPPRYTEASLVK
ALEKYGIGRPSTYATIISTLQQREYVEVDKRRFIPTDKGRIVNKFLTEYFKKYVEYSYTAGLEKELDEIAHHKNDYLSVL
NNFWHPFIERINKISEDVSRKDVVQEELEEDCPECGSKLSLRLGKNGRFIGCTNYPTCKYTRPVDSDPKEVIKEEPIVVE
DRKCPKCNSDLHIKQGRYGKFIGCSNYPECKHMEPLEKPKDTGAVCPKCNKNHIVEKKSRKGKVFYACAGFPKCKNAYWY
PPIKEECPKCHSPILLHKITKKDGEQKACPNSECDYAVAFETK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=591, Percent_Identity=26.0575296108291, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI4507635, Length=617, Percent_Identity=24.4732576985413, Blast_Score=127, Evalue=5e-29,
Organism=Escherichia coli, GI1787529, Length=821, Percent_Identity=35.6881851400731, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI48994931, Length=173, Percent_Identity=43.3526011560694, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1788061, Length=563, Percent_Identity=25.9325044404973, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=782, Percent_Identity=25.3196930946292, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17555378, Length=465, Percent_Identity=27.9569892473118, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323263, Length=510, Percent_Identity=24.5098039215686, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24585251, Length=630, Percent_Identity=25.5555555555556, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24640096, Length=586, Percent_Identity=22.8668941979522, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 87224; Mature: 87093

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNLVIVESPAKTKTIKKYLGNEFEILASFGHVREIPSKDTSIDVNDNFKIKFTTSEKS
CCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCHHH
KKHLDAIKKAAKDAQNIYLATDPDREGEAISWHVQEVLKSARLLKDKNIYRVTFNEITKS
HHHHHHHHHHHHHHCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
AVTNAIANPKELSMDLVDAQKARQALDFLVGFNISPLLWRKITSGLSAGRVQSPALRMIV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHH
EREIERENFIKQDYWSLTADTFKQKQIFANLLEFNNNKVEQFTFTTAEDAENAKAHILKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEC
ANGFLIVDGITEKKTRRNPYPPFITSTLQQEASKKLGFTAKRTMSTAQKLYEGIDLGNGE
CCCEEEEECCCCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
SVGLISYMRTDSTNLSNDALNDIRNFIQAKYDKDMLPAKPRVFEKKSQNAQEAHEAIRVT
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHEEEE
AANRTPESIKQFLTNDEFKLYSLIYNRTIACQMKHATLNSTSVDLITENTKHRFRVTGTV
ECCCCHHHHHHHHCCCCHHHHHHHHCCEEEEEEECCCCCCCCEEEEECCCCCEEEEEEEE
IVDAGFLKIYNVEKDEDEKDSDDEQTLPKFEKGEKIILNDVITKAHSTEPPPRYTEASLV
EEECCEEEEEECCCCCCCCCCCHHHHCCCHHCCCEEEHHHHHHHHCCCCCCCCCHHHHHH
KALEKYGIGRPSTYATIISTLQQREYVEVDKRRFIPTDKGRIVNKFLTEYFKKYVEYSYT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGLEKELDEIAHHKNDYLSVLNNFWHPFIERINKISEDVSRKDVVQEELEEDCPECGSKL
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
SLRLGKNGRFIGCTNYPTCKYTRPVDSDPKEVIKEEPIVVEDRKCPKCNSDLHIKQGRYG
EEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCEECCCCCC
KFIGCSNYPECKHMEPLEKPKDTGAVCPKCNKNHIVEKKSRKGKVFYACAGFPKCKNAYW
CEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
YPPIKEECPKCHSPILLHKITKKDGEQKACPNSECDYAVAFETK
CCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
AKNLVIVESPAKTKTIKKYLGNEFEILASFGHVREIPSKDTSIDVNDNFKIKFTTSEKS
CCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCHHH
KKHLDAIKKAAKDAQNIYLATDPDREGEAISWHVQEVLKSARLLKDKNIYRVTFNEITKS
HHHHHHHHHHHHHHCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
AVTNAIANPKELSMDLVDAQKARQALDFLVGFNISPLLWRKITSGLSAGRVQSPALRMIV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHH
EREIERENFIKQDYWSLTADTFKQKQIFANLLEFNNNKVEQFTFTTAEDAENAKAHILKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEC
ANGFLIVDGITEKKTRRNPYPPFITSTLQQEASKKLGFTAKRTMSTAQKLYEGIDLGNGE
CCCEEEEECCCCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
SVGLISYMRTDSTNLSNDALNDIRNFIQAKYDKDMLPAKPRVFEKKSQNAQEAHEAIRVT
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHEEEE
AANRTPESIKQFLTNDEFKLYSLIYNRTIACQMKHATLNSTSVDLITENTKHRFRVTGTV
ECCCCHHHHHHHHCCCCHHHHHHHHCCEEEEEEECCCCCCCCEEEEECCCCCEEEEEEEE
IVDAGFLKIYNVEKDEDEKDSDDEQTLPKFEKGEKIILNDVITKAHSTEPPPRYTEASLV
EEECCEEEEEECCCCCCCCCCCHHHHCCCHHCCCEEEHHHHHHHHCCCCCCCCCHHHHHH
KALEKYGIGRPSTYATIISTLQQREYVEVDKRRFIPTDKGRIVNKFLTEYFKKYVEYSYT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGLEKELDEIAHHKNDYLSVLNNFWHPFIERINKISEDVSRKDVVQEELEEDCPECGSKL
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
SLRLGKNGRFIGCTNYPTCKYTRPVDSDPKEVIKEEPIVVEDRKCPKCNSDLHIKQGRYG
EEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCEECCCCCC
KFIGCSNYPECKHMEPLEKPKDTGAVCPKCNKNHIVEKKSRKGKVFYACAGFPKCKNAYW
CEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
YPPIKEECPKCHSPILLHKITKKDGEQKACPNSECDYAVAFETK
CCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]