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The map label for this gene is rpoH [H]

Identifier: 134302102

GI number: 134302102

Start: 1083248

End: 1084126

Strand: Reverse

Name: rpoH [H]

Synonym: FTW_1144

Alternate gene names: 134302102

Gene position: 1084126-1083248 (Counterclockwise)

Preceding gene: 134302103

Following gene: 134302101

Centisome position: 57.11

GC content: 35.49

Gene sequence:

>879_bases
ATGGCTAACAAAAAATTATTACCAGCTACGAAGACGAAAACTTTACCAGTTGTATCTGATAATAATCTCAGTGCGTATTT
AAATTTTGTCAATACTTTACCAGTATTATCACTAGAGCAAGAGCAAGAGCTAGCAAGACGCTATAAATATAAAAAAGATC
TAGATGCTGCACAGCAACTTGTGTTATCACATTTACGCTTTGTTACTAAAATAGCAAGAAATTTTTCAGGTTATGGACTT
TCAATAGCGGACCTTATCCAAGAGGGTAATATTGGTCTTATGAAAGCTGTAAGTAAGTTTGATCCAGATCAAGGTGTTAG
ATTACTTTCATTTGCAGTTCATTGGATTAAGGCGGAAATGCATGACTATGTCCTAAAAAACTGGAAGATAGTCAAGGTTG
CTACCACAAAAGCACAGCGTAAATTATTTTTTAATCTTAGAAGTAGTAAAGACAAAATTGGTTGGTTAAGCTCGGAGAAT
ATCAAAGAGTTAGCTGAAGAGCTAGGTGTCAAAGAAGAAACTGTCATTGAGATGGAGAAAAGAATGTGTCAGGGGGATGC
AAGTCTTGATTTACCATACACTGATGATGACGGTGAGCAAACTTCGCAGCAAAGTTTGTATCTAGAGGATAAGTCTTCAA
ACATTGAGCATCAAGTTGTTCAGCAAGATTACTATGATAATTTCAAAGCGATTGTAAAAGATGTTTTAAGTGGTTTTGAT
ACACGTACAAAAGATATTATCATGTCGCGCTATCTACTTGATAACAAGGCAACTCTTCAGGATCTAGCTGCTAAATATAA
TATCTCAGCCGAAAGAGTACGCCAAATTGAAGAAGATGCTCTAGCTAAGCTTAAAAAAGCTATCAAGAATCGCTCTTAA

Upstream 100 bases:

>100_bases
GACAAGAGTTTGCGTATGTATTAGAGCAGCAACAAGCTCAAATTAATCAGCTAAAACAAGCGTACTACTATCAGTAGTAT
AAGCTAACGATAGGAGCCAC

Downstream 100 bases:

>100_bases
TATTTTGTCTATTTAGATAAATAGGTTCTGTGCACAAAAATAAATATTCATCGTAACCAAATAAAAGTACAAGCTAATTT
AATCATTCCTATATATGCTG

Product: RNA polymerase factor sigma-32

Products: diphosphate; RNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MANKKLLPATKTKTLPVVSDNNLSAYLNFVNTLPVLSLEQEQELARRYKYKKDLDAAQQLVLSHLRFVTKIARNFSGYGL
SIADLIQEGNIGLMKAVSKFDPDQGVRLLSFAVHWIKAEMHDYVLKNWKIVKVATTKAQRKLFFNLRSSKDKIGWLSSEN
IKELAEELGVKEETVIEMEKRMCQGDASLDLPYTDDDGEQTSQQSLYLEDKSSNIEHQVVQQDYYDNFKAIVKDVLSGFD
TRTKDIIMSRYLLDNKATLQDLAAKYNISAERVRQIEEDALAKLKKAIKNRS

Sequences:

>Translated_292_residues
MANKKLLPATKTKTLPVVSDNNLSAYLNFVNTLPVLSLEQEQELARRYKYKKDLDAAQQLVLSHLRFVTKIARNFSGYGL
SIADLIQEGNIGLMKAVSKFDPDQGVRLLSFAVHWIKAEMHDYVLKNWKIVKVATTKAQRKLFFNLRSSKDKIGWLSSEN
IKELAEELGVKEETVIEMEKRMCQGDASLDLPYTDDDGEQTSQQSLYLEDKSSNIEHQVVQQDYYDNFKAIVKDVLSGFD
TRTKDIIMSRYLLDNKATLQDLAAKYNISAERVRQIEEDALAKLKKAIKNRS
>Mature_291_residues
ANKKLLPATKTKTLPVVSDNNLSAYLNFVNTLPVLSLEQEQELARRYKYKKDLDAAQQLVLSHLRFVTKIARNFSGYGLS
IADLIQEGNIGLMKAVSKFDPDQGVRLLSFAVHWIKAEMHDYVLKNWKIVKVATTKAQRKLFFNLRSSKDKIGWLSSENI
KELAEELGVKEETVIEMEKRMCQGDASLDLPYTDDDGEQTSQQSLYLEDKSSNIEHQVVQQDYYDNFKAIVKDVLSGFDT
RTKDIIMSRYLLDNKATLQDLAAKYNISAERVRQIEEDALAKLKKAIKNRS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=52.5, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI1789098, Length=272, Percent_Identity=29.7794117647059, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789448, Length=255, Percent_Identity=28.2352941176471, Blast_Score=100, Evalue=8e-23,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: 2.7.7.6

Molecular weight: Translated: 33375; Mature: 33243

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKKLLPATKTKTLPVVSDNNLSAYLNFVNTLPVLSLEQEQELARRYKYKKDLDAAQQL
CCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VLSHLRFVTKIARNFSGYGLSIADLIQEGNIGLMKAVSKFDPDQGVRLLSFAVHWIKAEM
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
HDYVLKNWKIVKVATTKAQRKLFFNLRSSKDKIGWLSSENIKELAEELGVKEETVIEMEK
HHHHHHCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
RMCQGDASLDLPYTDDDGEQTSQQSLYLEDKSSNIEHQVVQQDYYDNFKAIVKDVLSGFD
HHHCCCCCCCCCCCCCCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
TRTKDIIMSRYLLDNKATLQDLAAKYNISAERVRQIEEDALAKLKKAIKNRS
CHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANKKLLPATKTKTLPVVSDNNLSAYLNFVNTLPVLSLEQEQELARRYKYKKDLDAAQQL
CCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VLSHLRFVTKIARNFSGYGLSIADLIQEGNIGLMKAVSKFDPDQGVRLLSFAVHWIKAEM
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
HDYVLKNWKIVKVATTKAQRKLFFNLRSSKDKIGWLSSENIKELAEELGVKEETVIEMEK
HHHHHHCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
RMCQGDASLDLPYTDDDGEQTSQQSLYLEDKSSNIEHQVVQQDYYDNFKAIVKDVLSGFD
HHHCCCCCCCCCCCCCCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
TRTKDIIMSRYLLDNKATLQDLAAKYNISAERVRQIEEDALAKLKKAIKNRS
CHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside triphosphate; RNAn

Specific reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]