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The map label for this gene is tyrS [H]

Identifier: 134302006

GI number: 134302006

Start: 979900

End: 981120

Strand: Reverse

Name: tyrS [H]

Synonym: FTW_1035

Alternate gene names: 134302006

Gene position: 981120-979900 (Counterclockwise)

Preceding gene: 134302008

Following gene: 134302004

Centisome position: 51.68

GC content: 33.99

Gene sequence:

>1221_bases
TTGAGAGAAATTTTTAATATTATAAATATAATGTCGAGCATAAAAGAGACTTTAGAAATTATCAAAAGAGGGGCGGATGA
GGTCCTAATCGAAGAAGAATTAATCAAAAAATTACAAAAACATAAGCCATTAATAATTAAATTTGGTTGCGACCCAACTG
CTCCAGATATTCATTTGGGACATACTGTAGTTATCAATAAGCTAAAGCAATTACAAGATTTGGGACATGAAATTCACTTT
TTGATCGGGGATTTTACAGCTCAGATAGGTGATCCTACAGGTAAAAATGCAACAAGACCGCCATTAACTGCCGAAGAAGT
TGCGGCAAATGCTGAAACTTATACTAAGCAAGTATTTAAGATTCTTGATAAAGACAAAACTGTTATTCGTCGTAATGGTG
ATTGGTTTAACAAAATGTCTGCTAGTGAGATGATCAAACTAGCTTCTAAATCAACGGTTGCAAGAATGCTTGAAAGAGAT
GATTTCTCAAAAAGATATAAAGGCGGACAGTCGATCTCAATACATGAGTTTTTATATCCATTAGTACAAGGATATGATTC
TGTAGCTATGAATGCTGATATTGAGCTTGGAGGTACAGATCAAAAATTTAACCTACTAATGGGTAGAGAGCTGCAAAAGC
AGCAGGGTCAAGAACCACAAGTTATTATCACAATGCCACTTTTAGAAGGTTTAGATGGCGTCAAAAAAATGTCTAAATCA
AGCCAAAACTATATCGGTATCGAAGAACCAGCAAATGAAATCTTTGGTAAGATAATGTCAATTTCAGATGAACTTATGTG
GCGCTATTATGAATTACTAAGTTTCAAATCTTTAGAAAATATCGCTAAATTAAAGCAAGATGTTGCAGCTGGAGCTAATC
CGCGCGATATTAAGATAGAGCTGGCAAAAGAGTTAATTGAAAGGTTTCACTCTAAAGAAGATGCTGAGAGTGCGCACCAG
GATTTTATTCAAAGATTCCAAAAAAATCAGATACCTGATGATATAAATGTTGTAGAATTAAACCAAGAGCTACCGATAGC
AAACTTATTAAAAGAAGCAGGCTTAGTTGCAAGTACTTCTGAAGCTAATCGTATGATTCAGCAAGGTGCTGTTAAAATCG
ATGGTGAAAAGCTTAGTGATGCTAAGATTATTTTTGCTAAAGGAACAAATAATGTTTTCCAAGTAGGAAAACGTAAATTT
GCAAAAATTATTATAAAATGA

Upstream 100 bases:

>100_bases
GATATGTATATATGCATAGGATATTAGATATTTTTACTTTCTAATAAGTATAAGGTTTTATTGGTTCTATAAAAGAGTTT
TGTGTTATAATTTTGCTTTA

Downstream 100 bases:

>100_bases
GGGGTTGTAAGATGGATAAGTTTTTATCAATATTTGTTGGCGTATTAATCATAGTAACTATTGTAACTATATTTATTCAC
CTGTTTCCTATAGCTATAAA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MREIFNIINIMSSIKETLEIIKRGADEVLIEEELIKKLQKHKPLIIKFGCDPTAPDIHLGHTVVINKLKQLQDLGHEIHF
LIGDFTAQIGDPTGKNATRPPLTAEEVAANAETYTKQVFKILDKDKTVIRRNGDWFNKMSASEMIKLASKSTVARMLERD
DFSKRYKGGQSISIHEFLYPLVQGYDSVAMNADIELGGTDQKFNLLMGRELQKQQGQEPQVIITMPLLEGLDGVKKMSKS
SQNYIGIEEPANEIFGKIMSISDELMWRYYELLSFKSLENIAKLKQDVAAGANPRDIKIELAKELIERFHSKEDAESAHQ
DFIQRFQKNQIPDDINVVELNQELPIANLLKEAGLVASTSEANRMIQQGAVKIDGEKLSDAKIIFAKGTNNVFQVGKRKF
AKIIIK

Sequences:

>Translated_406_residues
MREIFNIINIMSSIKETLEIIKRGADEVLIEEELIKKLQKHKPLIIKFGCDPTAPDIHLGHTVVINKLKQLQDLGHEIHF
LIGDFTAQIGDPTGKNATRPPLTAEEVAANAETYTKQVFKILDKDKTVIRRNGDWFNKMSASEMIKLASKSTVARMLERD
DFSKRYKGGQSISIHEFLYPLVQGYDSVAMNADIELGGTDQKFNLLMGRELQKQQGQEPQVIITMPLLEGLDGVKKMSKS
SQNYIGIEEPANEIFGKIMSISDELMWRYYELLSFKSLENIAKLKQDVAAGANPRDIKIELAKELIERFHSKEDAESAHQ
DFIQRFQKNQIPDDINVVELNQELPIANLLKEAGLVASTSEANRMIQQGAVKIDGEKLSDAKIIFAKGTNNVFQVGKRKF
AKIIIK
>Mature_406_residues
MREIFNIINIMSSIKETLEIIKRGADEVLIEEELIKKLQKHKPLIIKFGCDPTAPDIHLGHTVVINKLKQLQDLGHEIHF
LIGDFTAQIGDPTGKNATRPPLTAEEVAANAETYTKQVFKILDKDKTVIRRNGDWFNKMSASEMIKLASKSTVARMLERD
DFSKRYKGGQSISIHEFLYPLVQGYDSVAMNADIELGGTDQKFNLLMGRELQKQQGQEPQVIITMPLLEGLDGVKKMSKS
SQNYIGIEEPANEIFGKIMSISDELMWRYYELLSFKSLENIAKLKQDVAAGANPRDIKIELAKELIERFHSKEDAESAHQ
DFIQRFQKNQIPDDINVVELNQELPIANLLKEAGLVASTSEANRMIQQGAVKIDGEKLSDAKIIFAKGTNNVFQVGKRKF
AKIIIK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=408, Percent_Identity=25, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1787925, Length=431, Percent_Identity=27.1461716937355, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25145802, Length=367, Percent_Identity=24.7956403269755, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325160, Length=388, Percent_Identity=25, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI20130383, Length=400, Percent_Identity=22.5, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45809; Mature: 45809

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIFNIINIMSSIKETLEIIKRGADEVLIEEELIKKLQKHKPLIIKFGCDPTAPDIHLG
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
HTVVINKLKQLQDLGHEIHFLIGDFTAQIGDPTGKNATRPPLTAEEVAANAETYTKQVFK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHH
ILDKDKTVIRRNGDWFNKMSASEMIKLASKSTVARMLERDDFSKRYKGGQSISIHEFLYP
HHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEHHHHHHH
LVQGYDSVAMNADIELGGTDQKFNLLMGRELQKQQGQEPQVIITMPLLEGLDGVKKMSKS
HHHCCCHHEECCCEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHCHHHHHHHHHC
SQNYIGIEEPANEIFGKIMSISDELMWRYYELLSFKSLENIAKLKQDVAAGANPRDIKIE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LAKELIERFHSKEDAESAHQDFIQRFQKNQIPDDINVVELNQELPIANLLKEAGLVASTS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCH
EANRMIQQGAVKIDGEKLSDAKIIFAKGTNNVFQVGKRKFAKIIIK
HHHHHHHHCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MREIFNIINIMSSIKETLEIIKRGADEVLIEEELIKKLQKHKPLIIKFGCDPTAPDIHLG
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
HTVVINKLKQLQDLGHEIHFLIGDFTAQIGDPTGKNATRPPLTAEEVAANAETYTKQVFK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHH
ILDKDKTVIRRNGDWFNKMSASEMIKLASKSTVARMLERDDFSKRYKGGQSISIHEFLYP
HHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEHHHHHHH
LVQGYDSVAMNADIELGGTDQKFNLLMGRELQKQQGQEPQVIITMPLLEGLDGVKKMSKS
HHHCCCHHEECCCEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHCHHHHHHHHHC
SQNYIGIEEPANEIFGKIMSISDELMWRYYELLSFKSLENIAKLKQDVAAGANPRDIKIE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LAKELIERFHSKEDAESAHQDFIQRFQKNQIPDDINVVELNQELPIANLLKEAGLVASTS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCH
EANRMIQQGAVKIDGEKLSDAKIIFAKGTNNVFQVGKRKFAKIIIK
HHHHHHHHCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA