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The map label for this gene is truA [H]

Identifier: 134301921

GI number: 134301921

Start: 893482

End: 894258

Strand: Reverse

Name: truA [H]

Synonym: FTW_0928

Alternate gene names: 134301921

Gene position: 894258-893482 (Counterclockwise)

Preceding gene: 134301922

Following gene: 134301920

Centisome position: 47.1

GC content: 33.59

Gene sequence:

>777_bases
ATGAAAAACTACCTACTACAAATCGAATACTTTGGCAAAAACTACTGTGGTTGGCAAAGACAATCTCATTCACCAAGTGT
GCAAGAATCGCTTGAAAAAGCACTTTCAAAAATAGCAAATCAAAATATAGAAGTTACTTGTGCAGGGCGAACTGATACAG
GCGTGCATGCAACTTCACAGATTGTTAATTTTTATTCTAATGCAGATAGGCCTTTAAGCGCATGGCAGCGTGGGGTAAAT
GCTTTGCTACCTCAAGATATTAAAATATTAGCAGTTCAACAAGTTGATAATAATTTTAATTCTAGGTTTACAGCTATAAA
TAGAACTTACAATTATATTATCTACAATTCTGCAACTAGCTCACCAATATTCGCGGAACATTGCTTATGGGAGAATAGAG
AACTTGATATAGATAAGATGAATCAAGCTTGTGAATATCTTCTTGGCGAACAAGATTTTAGTTCTTTTAGATCATCACAA
TGTCAGTCAAATACACCATTTAGAAATATCCAAAAAGCTGAGTTTATCAAGCAGGGTAGTTTTATTGTTTTTGAGGTTGT
TGGTAATGCTTTTTTACATCATATGATTAGAAATTTAGTAGGCTCATTATTAAAAGTAGGCTTAGGTTTTGAATCTCCAG
AATGGATTAAAGTAGTATTAGAAGCTAAAGATAGAACACAAGCAGCTGAGACAGCTAAAGCACATGGTTTGTATTTTGTC
GGTGTTGAATATCCGGAATTTAGCTTCAAACGGCAAATAATTAAATTGTTTTGTTAG

Upstream 100 bases:

>100_bases
ATTTTGATAGTTTGGTTCATGGTTTTATCAATTTCTCAAAACTTATTCCAAAAGAAATGGATGTTCTGCATTCTAGAGTT
GTTAAATTTCTTGGCTAATT

Downstream 100 bases:

>100_bases
GTTTAATCTGATAATAATTATTCAATAGATATAAAATTATAAATAAAGGATAGCGATGAAAAAACTATCTACAATGGCAG
TTGTGTTTATTTCCACTGGG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKNYLLQIEYFGKNYCGWQRQSHSPSVQESLEKALSKIANQNIEVTCAGRTDTGVHATSQIVNFYSNADRPLSAWQRGVN
ALLPQDIKILAVQQVDNNFNSRFTAINRTYNYIIYNSATSSPIFAEHCLWENRELDIDKMNQACEYLLGEQDFSSFRSSQ
CQSNTPFRNIQKAEFIKQGSFIVFEVVGNAFLHHMIRNLVGSLLKVGLGFESPEWIKVVLEAKDRTQAAETAKAHGLYFV
GVEYPEFSFKRQIIKLFC

Sequences:

>Translated_258_residues
MKNYLLQIEYFGKNYCGWQRQSHSPSVQESLEKALSKIANQNIEVTCAGRTDTGVHATSQIVNFYSNADRPLSAWQRGVN
ALLPQDIKILAVQQVDNNFNSRFTAINRTYNYIIYNSATSSPIFAEHCLWENRELDIDKMNQACEYLLGEQDFSSFRSSQ
CQSNTPFRNIQKAEFIKQGSFIVFEVVGNAFLHHMIRNLVGSLLKVGLGFESPEWIKVVLEAKDRTQAAETAKAHGLYFV
GVEYPEFSFKRQIIKLFC
>Mature_258_residues
MKNYLLQIEYFGKNYCGWQRQSHSPSVQESLEKALSKIANQNIEVTCAGRTDTGVHATSQIVNFYSNADRPLSAWQRGVN
ALLPQDIKILAVQQVDNNFNSRFTAINRTYNYIIYNSATSSPIFAEHCLWENRELDIDKMNQACEYLLGEQDFSSFRSSQ
CQSNTPFRNIQKAEFIKQGSFIVFEVVGNAFLHHMIRNLVGSLLKVGLGFESPEWIKVVLEAKDRTQAAETAKAHGLYFV
GVEYPEFSFKRQIIKLFC

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=27.2058823529412, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI199559785, Length=282, Percent_Identity=25.531914893617, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788657, Length=241, Percent_Identity=50.207468879668, Blast_Score=252, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321112, Length=276, Percent_Identity=27.536231884058, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648455, Length=235, Percent_Identity=26.8085106382979, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281362146, Length=235, Percent_Identity=26.8085106382979, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922728, Length=265, Percent_Identity=26.4150943396226, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29489; Mature: 29489

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYLLQIEYFGKNYCGWQRQSHSPSVQESLEKALSKIANQNIEVTCAGRTDTGVHATSQ
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
IVNFYSNADRPLSAWQRGVNALLPQDIKILAVQQVDNNFNSRFTAINRTYNYIIYNSATS
HHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCEEEEECEEEEEEECCCCC
SPIFAEHCLWENRELDIDKMNQACEYLLGEQDFSSFRSSQCQSNTPFRNIQKAEFIKQGS
CCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
FIVFEVVGNAFLHHMIRNLVGSLLKVGLGFESPEWIKVVLEAKDRTQAAETAKAHGLYFV
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEHHHHHHHHHHHHHHCCEEEE
GVEYPEFSFKRQIIKLFC
EECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKNYLLQIEYFGKNYCGWQRQSHSPSVQESLEKALSKIANQNIEVTCAGRTDTGVHATSQ
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
IVNFYSNADRPLSAWQRGVNALLPQDIKILAVQQVDNNFNSRFTAINRTYNYIIYNSATS
HHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCEEEEECEEEEEEECCCCC
SPIFAEHCLWENRELDIDKMNQACEYLLGEQDFSSFRSSQCQSNTPFRNIQKAEFIKQGS
CCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
FIVFEVVGNAFLHHMIRNLVGSLLKVGLGFESPEWIKVVLEAKDRTQAAETAKAHGLYFV
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEHHHHHHHHHHHHHHCCEEEE
GVEYPEFSFKRQIIKLFC
EECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA