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The map label for this gene is mscM [H]

Identifier: 134301896

GI number: 134301896

Start: 863625

End: 864815

Strand: Direct

Name: mscM [H]

Synonym: FTW_0899

Alternate gene names: 134301896

Gene position: 863625-864815 (Clockwise)

Preceding gene: 134301895

Following gene: 134301899

Centisome position: 45.49

GC content: 28.8

Gene sequence:

>1191_bases
ATGTCGTTACTAAAAAAATACTATTTTGATATTATTAATAACCATGGATTTTCTGTAGCTTTAAGCTGTTTAACTATTAT
TCTAATAGTACTATTATTATCATTTATCATTAATAGATTATCATCGAAATATATAGTTTTGATTGCTAAAAAATTCTTTG
ATAAAAGTAATTCCATGCTAGGAAAATACCTATTAGAATATAAGTTTTTTGTTAAGTTATCTCATATTATTCCAGGTATA
TTTGCCTATGTTATAATAGGTTTTGCTACTGATAATGATTATCTTTGGACACAACATTTAGTCACTGGTTTACAGTTATT
ATTACAAATTTATATAACTGTTACTATAATTTCATTTTTAGTATGTTTTGTAGATGCTCTTTTTGACTACCTTGAGAATC
TTGCTTATTTTAAAAATCATTCATTACGTAGCTATGCTCAAGTAATCAAGATTATCTTATACATAATAGGCTTTATCTTA
GTAGTATCGTTATTGTTAAATAAATCACCTATTGCCTTTTTAACGGGATTAGGAGCATTATCAGCAGTTCTTATGTTGGT
CTTTAAGGATACAATATTAGGCTTTGTAACCAATGTTCAAGTTGCCGCTCTTGATTTAGTAAGAGTTGGAGATTGGATTA
CTATCCCATCTGCTAATGTTGATGGTACTGTTATGGATGTATCTATTAATACAGTAAAAATCCGTAATTTTGATAAAACT
ATATCGATGATACCAACTTATACACTAACTACTCATAGTGTCCAAAATTGGCGTGGTATGGTAGAAACCGGAGGTCGTCG
TATTAAGCGCTCGATAAATATAGATATAGATACGATAAAATTCTGTGATCAAGAGCTTCTAGAAAGACTAGGCAAAGAAA
CTCTTTTAGCGGATTTTATCAAAGCTAAAGCTAATGAAAAACTTACTAATATTAGTCTATTTCGTGCTTATATTGAAAAC
TATCTGAGAAATCATCCTAAGATACATTTAGGTTTGACATTTTTAATTAGGGAGCTTCAGCCGACACAGTTTGGCTTACC
TGTTGAGTTATATATATTTACAAATGACACTAACTGGGTTAACTATGAGAAAATTCAAGCTGATATTTTTGATTATGTAT
TTGCTAGTTTACATATGTTTGACTTAAAAGCTTTCCAAGCTATTACTGGTAGAATTAATAATCAATTTTAA

Upstream 100 bases:

>100_bases
AACTCAAGCATCAACTTCTAGTGATAATTCGTTACAATAAATTCAATAATTATTTTGTTTTATAATAACAATATCTAACT
TAAAGGTAATATAAAATATA

Downstream 100 bases:

>100_bases
AAAACTTTAGCATTTAGGTAATGTAACGCCAACTTGACCTTGGTATTTACCGCCTTTATCAGCATAAGAAGTTTCACACT
CTTCATCTGACTGAAAAAAT

Product: mechanosensitive ion channel family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSLLKKYYFDIINNHGFSVALSCLTIILIVLLLSFIINRLSSKYIVLIAKKFFDKSNSMLGKYLLEYKFFVKLSHIIPGI
FAYVIIGFATDNDYLWTQHLVTGLQLLLQIYITVTIISFLVCFVDALFDYLENLAYFKNHSLRSYAQVIKIILYIIGFIL
VVSLLLNKSPIAFLTGLGALSAVLMLVFKDTILGFVTNVQVAALDLVRVGDWITIPSANVDGTVMDVSINTVKIRNFDKT
ISMIPTYTLTTHSVQNWRGMVETGGRRIKRSINIDIDTIKFCDQELLERLGKETLLADFIKAKANEKLTNISLFRAYIEN
YLRNHPKIHLGLTFLIRELQPTQFGLPVELYIFTNDTNWVNYEKIQADIFDYVFASLHMFDLKAFQAITGRINNQF

Sequences:

>Translated_396_residues
MSLLKKYYFDIINNHGFSVALSCLTIILIVLLLSFIINRLSSKYIVLIAKKFFDKSNSMLGKYLLEYKFFVKLSHIIPGI
FAYVIIGFATDNDYLWTQHLVTGLQLLLQIYITVTIISFLVCFVDALFDYLENLAYFKNHSLRSYAQVIKIILYIIGFIL
VVSLLLNKSPIAFLTGLGALSAVLMLVFKDTILGFVTNVQVAALDLVRVGDWITIPSANVDGTVMDVSINTVKIRNFDKT
ISMIPTYTLTTHSVQNWRGMVETGGRRIKRSINIDIDTIKFCDQELLERLGKETLLADFIKAKANEKLTNISLFRAYIEN
YLRNHPKIHLGLTFLIRELQPTQFGLPVELYIFTNDTNWVNYEKIQADIFDYVFASLHMFDLKAFQAITGRINNQF
>Mature_395_residues
SLLKKYYFDIINNHGFSVALSCLTIILIVLLLSFIINRLSSKYIVLIAKKFFDKSNSMLGKYLLEYKFFVKLSHIIPGIF
AYVIIGFATDNDYLWTQHLVTGLQLLLQIYITVTIISFLVCFVDALFDYLENLAYFKNHSLRSYAQVIKIILYIIGFILV
VSLLLNKSPIAFLTGLGALSAVLMLVFKDTILGFVTNVQVAALDLVRVGDWITIPSANVDGTVMDVSINTVKIRNFDKTI
SMIPTYTLTTHSVQNWRGMVETGGRRIKRSINIDIDTIKFCDQELLERLGKETLLADFIKAKANEKLTNISLFRAYIENY
LRNHPKIHLGLTFLIRELQPTQFGLPVELYIFTNDTNWVNYEKIQADIFDYVFASLHMFDLKAFQAITGRINNQF

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=385, Percent_Identity=36.6233766233766, Blast_Score=268, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 45346; Mature: 45215

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLKKYYFDIINNHGFSVALSCLTIILIVLLLSFIINRLSSKYIVLIAKKFFDKSNSML
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCHHHH
GKYLLEYKFFVKLSHIIPGIFAYVIIGFATDNDYLWTQHLVTGLQLLLQIYITVTIISFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VCFVDALFDYLENLAYFKNHSLRSYAQVIKIILYIIGFILVVSLLLNKSPIAFLTGLGAL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SAVLMLVFKDTILGFVTNVQVAALDLVRVGDWITIPSANVDGTVMDVSINTVKIRNFDKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEEEEEEECCCHH
ISMIPTYTLTTHSVQNWRGMVETGGRRIKRSINIDIDTIKFCDQELLERLGKETLLADFI
HHHCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHH
KAKANEKLTNISLFRAYIENYLRNHPKIHLGLTFLIRELQPTQFGLPVELYIFTNDTNWV
HHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCCCEEEEEEECCCCCC
NYEKIQADIFDYVFASLHMFDLKAFQAITGRINNQF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLLKKYYFDIINNHGFSVALSCLTIILIVLLLSFIINRLSSKYIVLIAKKFFDKSNSML
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCHHHH
GKYLLEYKFFVKLSHIIPGIFAYVIIGFATDNDYLWTQHLVTGLQLLLQIYITVTIISFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VCFVDALFDYLENLAYFKNHSLRSYAQVIKIILYIIGFILVVSLLLNKSPIAFLTGLGAL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SAVLMLVFKDTILGFVTNVQVAALDLVRVGDWITIPSANVDGTVMDVSINTVKIRNFDKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEEEEEEECCCHH
ISMIPTYTLTTHSVQNWRGMVETGGRRIKRSINIDIDTIKFCDQELLERLGKETLLADFI
HHHCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHH
KAKANEKLTNISLFRAYIENYLRNHPKIHLGLTFLIRELQPTQFGLPVELYIFTNDTNWV
HHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCCCEEEEEEECCCCCC
NYEKIQADIFDYVFASLHMFDLKAFQAITGRINNQF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]