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The map label for this gene is shiA [H]

Identifier: 134301899

GI number: 134301899

Start: 866334

End: 867566

Strand: Direct

Name: shiA [H]

Synonym: FTW_0902

Alternate gene names: 134301899

Gene position: 866334-867566 (Clockwise)

Preceding gene: 134301896

Following gene: 134301900

Centisome position: 45.63

GC content: 31.31

Gene sequence:

>1233_bases
ATGCTTAAAAAAGATGATTTTAAAACGATTTTGCTTACAAGTATTGGTGGAATGTTAGAATTTTATGACTTTGTCATATT
TGGTATGTTTGCTATCGTGCTTGGTAAAACATTTTTCCCTCCCGAAGGCTCGCCAGCATTGCAAGCACTTTCTGCATTTA
CAGTTTTTGCTGTTGGATACTTTGCTAGACCATTTGGTGGAATTATTTTTGGTCATATAGGTGATAAATATGGACGTAAA
AAATCTTTCTTACTGACAATACTACTTATGGGTTTAGCTGCTTTTATGATTGCTATATTACCCTCTTATCAAAATGTTGG
TATAATCGCACCACTATTATTTGTTATACTAAGAATCATTCAAGGTGCTGCGATTGGTGGTGAAATTCCAAGTGCTGTAG
TATTCGTCAAAGAATCACTACTAAAACATGGTGGTTTAGCTTGTGGAATTATATTTTGTTTTATCAATTTTGGGATATTT
TTTGCAGAGATCACTAAAATAGTAACTACATATTTCCTAAGTGATGACTATGCTTGGCGTGTTGCATTTATGTTTGGAGG
TATTGCTGCAATTATCAGTTATTTTTTTAGAAAAGAGATTCATGAGACTGCAATATTCTTAAATGAAAAAACGCATTATA
AAATTCCAATTATTGATTTATTCAAAACAGAGAAATTAGCAACAATTAGAGCTATTGCAGCTATTTCAATTTTTGCGATG
GTAGTTGGATTCTTCTCACTTTACTTGCCAACATACTTTGAACTAAATCACATTGCTAATAGTTCAAGTTTGATTCTTAT
TAATCTATTTGTTTTTTCTGTAGTTTCTATTCCAGCAGGTTTTCTTGCTGATAAATTTACACCACTACGTATACTTTTTA
TCGGTGCGATAGGTTTAGTAATTTTTGGAAGTATTTTTTATTATGGTATTGTTACTAACTCTAAATATTTACTTTTGATA
ATGCTAATTAACAATATTTTTATGGGATTAGTTGTAGGTGTTGCTTCTAATTATGCAAGTACCCTTTTTAGTCCTGGAGT
GAGGGCTAGTGGTTTAGGTTTCAGTTATAATATTAGTTTCGCTATTTTTAATGGTGCATTTTTAGCTTTGGCAAGTTTAG
GGATTGCTAAAGGTTTTATATTAACACCGCTATATCTAGTTTTAATAGTCATTTTTACCTCTACGCTTATCTTAGTCTTT
ACACGTAATACTTCACAAAACATCTCTATCTAA

Upstream 100 bases:

>100_bases
TTTGACAACATTTTCGATTCTTATCATATTAAACTATTAGCTTTTTATAATTTATTAGCTATTATAAAGACAATTTGAGT
GTAATTATACTTAATTAAAG

Downstream 100 bases:

>100_bases
CACACTAGTAACTGTGATAAAATTTTTTAAAAGGTCATTCTATTTTAATAGTAATGGAAAAATTCTTATCAAGTCTAATA
TATATTAGAAGCCAATACAA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MLKKDDFKTILLTSIGGMLEFYDFVIFGMFAIVLGKTFFPPEGSPALQALSAFTVFAVGYFARPFGGIIFGHIGDKYGRK
KSFLLTILLMGLAAFMIAILPSYQNVGIIAPLLFVILRIIQGAAIGGEIPSAVVFVKESLLKHGGLACGIIFCFINFGIF
FAEITKIVTTYFLSDDYAWRVAFMFGGIAAIISYFFRKEIHETAIFLNEKTHYKIPIIDLFKTEKLATIRAIAAISIFAM
VVGFFSLYLPTYFELNHIANSSSLILINLFVFSVVSIPAGFLADKFTPLRILFIGAIGLVIFGSIFYYGIVTNSKYLLLI
MLINNIFMGLVVGVASNYASTLFSPGVRASGLGFSYNISFAIFNGAFLALASLGIAKGFILTPLYLVLIVIFTSTLILVF
TRNTSQNISI

Sequences:

>Translated_410_residues
MLKKDDFKTILLTSIGGMLEFYDFVIFGMFAIVLGKTFFPPEGSPALQALSAFTVFAVGYFARPFGGIIFGHIGDKYGRK
KSFLLTILLMGLAAFMIAILPSYQNVGIIAPLLFVILRIIQGAAIGGEIPSAVVFVKESLLKHGGLACGIIFCFINFGIF
FAEITKIVTTYFLSDDYAWRVAFMFGGIAAIISYFFRKEIHETAIFLNEKTHYKIPIIDLFKTEKLATIRAIAAISIFAM
VVGFFSLYLPTYFELNHIANSSSLILINLFVFSVVSIPAGFLADKFTPLRILFIGAIGLVIFGSIFYYGIVTNSKYLLLI
MLINNIFMGLVVGVASNYASTLFSPGVRASGLGFSYNISFAIFNGAFLALASLGIAKGFILTPLYLVLIVIFTSTLILVF
TRNTSQNISI
>Mature_410_residues
MLKKDDFKTILLTSIGGMLEFYDFVIFGMFAIVLGKTFFPPEGSPALQALSAFTVFAVGYFARPFGGIIFGHIGDKYGRK
KSFLLTILLMGLAAFMIAILPSYQNVGIIAPLLFVILRIIQGAAIGGEIPSAVVFVKESLLKHGGLACGIIFCFINFGIF
FAEITKIVTTYFLSDDYAWRVAFMFGGIAAIISYFFRKEIHETAIFLNEKTHYKIPIIDLFKTEKLATIRAIAAISIFAM
VVGFFSLYLPTYFELNHIANSSSLILINLFVFSVVSIPAGFLADKFTPLRILFIGAIGLVIFGSIFYYGIVTNSKYLLLI
MLINNIFMGLVVGVASNYASTLFSPGVRASGLGFSYNISFAIFNGAFLALASLGIAKGFILTPLYLVLIVIFTSTLILVF
TRNTSQNISI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=375, Percent_Identity=30.1333333333333, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=27.2727272727273, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1788942, Length=417, Percent_Identity=27.0983213429257, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789941, Length=375, Percent_Identity=28.2666666666667, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI87082231, Length=218, Percent_Identity=25.2293577981651, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44968; Mature: 44968

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCC
>Mature Secondary Structure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EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]