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The map label for this gene is grpE

Identifier: 134301631

GI number: 134301631

Start: 564692

End: 565279

Strand: Direct

Name: grpE

Synonym: FTW_0570

Alternate gene names: 134301631

Gene position: 564692-565279 (Clockwise)

Preceding gene: 134301624

Following gene: 134301632

Centisome position: 29.74

GC content: 32.99

Gene sequence:

>588_bases
ATGAGTAAGCAAGAAAAAAGTAATGTAGAAGATAAAAGTCTTGATATTGAGACAGCAGCACAAGTAGAAACAGCACAAGA
ATCTGCTAGTGGAGCTTTAGAAGAATTATCTGTAGAAGAACAGCTAGAAAGAGCAAAAGATACTATAAAAGAACTTGAAG
ATAGTTGTGATCAATTTAAAGATGAGGCTTTAAGAGCGAAGGCTGAAATGGAAAACATTCGTAAAAGAGCTGAAAGAGAT
GTATCAAATGCACGCAAATTTGGTATAGAGAAGTTTTCTAAAGAGCTTTTGCCAGTAATTGATAGTATTGAGCAAGCATT
AAAGCATGAGGTTAAGCTTGAAGAAGCTATCGCAATGAAAGAAGGTATTGAGTTAACAGCAAAAATGCTGGTTGATATAC
TTAAGAAAAATGGTGTAGAAGAGTTAGATCCAAAAGGAGAGAAATTTGACCCTAATCTACATGAAGCTATGGCAATGATT
CCTAACCCTGAATTTGAAGATAATACTATTTTTGATGTTTTTCAAAAGGGTTATATGTTAAATGGTCGTATTGTTAGAGC
TGCAAAAGTTGTTATAGTAAAAAATTAA

Upstream 100 bases:

>100_bases
AAAAAATACCAATAATTTCTTGAAAATACAGAATTAATCACTATCTATAAAACATCTAACAAATTGTAGTATTAACAGAA
CTAAAATAAAAGGTTATTTT

Downstream 100 bases:

>100_bases
AAAAAATACTTGAAAAGATTATAAATATGCCCATCTAGTAAGTAGTCAGATGGATTTAAAATTTTTGGAAAAAATTACAG
AATAAAAACAGGAGAATCAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSKQEKSNVEDKSLDIETAAQVETAQESASGALEELSVEEQLERAKDTIKELEDSCDQFKDEALRAKAEMENIRKRAERD
VSNARKFGIEKFSKELLPVIDSIEQALKHEVKLEEAIAMKEGIELTAKMLVDILKKNGVEELDPKGEKFDPNLHEAMAMI
PNPEFEDNTIFDVFQKGYMLNGRIVRAAKVVIVKN

Sequences:

>Translated_195_residues
MSKQEKSNVEDKSLDIETAAQVETAQESASGALEELSVEEQLERAKDTIKELEDSCDQFKDEALRAKAEMENIRKRAERD
VSNARKFGIEKFSKELLPVIDSIEQALKHEVKLEEAIAMKEGIELTAKMLVDILKKNGVEELDPKGEKFDPNLHEAMAMI
PNPEFEDNTIFDVFQKGYMLNGRIVRAAKVVIVKN
>Mature_194_residues
SKQEKSNVEDKSLDIETAAQVETAQESASGALEELSVEEQLERAKDTIKELEDSCDQFKDEALRAKAEMENIRKRAERDV
SNARKFGIEKFSKELLPVIDSIEQALKHEVKLEEAIAMKEGIELTAKMLVDILKKNGVEELDPKGEKFDPNLHEAMAMIP
NPEFEDNTIFDVFQKGYMLNGRIVRAAKVVIVKN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=150, Percent_Identity=35.3333333333333, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI40255109, Length=152, Percent_Identity=33.5526315789474, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1788967, Length=136, Percent_Identity=47.0588235294118, Blast_Score=126, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17552458, Length=144, Percent_Identity=38.1944444444444, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=144, Percent_Identity=39.5833333333333, Blast_Score=108, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24653432, Length=139, Percent_Identity=37.410071942446, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_FRAT1 (Q14GW8)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_667358.1
- ProteinModelPortal:   Q14GW8
- SMR:   Q14GW8
- STRING:   Q14GW8
- GeneID:   4200300
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF1270c
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   EVEPNSV
- PhylomeDB:   Q14GW8
- ProtClustDB:   PRK14148
- BioCyc:   FTUL393115:FTF1270C-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22052; Mature: 21921

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQEKSNVEDKSLDIETAAQVETAQESASGALEELSVEEQLERAKDTIKELEDSCDQFK
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEALRAKAEMENIRKRAERDVSNARKFGIEKFSKELLPVIDSIEQALKHEVKLEEAIAMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGIELTAKMLVDILKKNGVEELDPKGEKFDPNLHEAMAMIPNPEFEDNTIFDVFQKGYML
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCC
NGRIVRAAKVVIVKN
CCEEEEHEEEEEECC
>Mature Secondary Structure 
SKQEKSNVEDKSLDIETAAQVETAQESASGALEELSVEEQLERAKDTIKELEDSCDQFK
CCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEALRAKAEMENIRKRAERDVSNARKFGIEKFSKELLPVIDSIEQALKHEVKLEEAIAMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGIELTAKMLVDILKKNGVEELDPKGEKFDPNLHEAMAMIPNPEFEDNTIFDVFQKGYML
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCC
NGRIVRAAKVVIVKN
CCEEEEHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA