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The map label for this gene is trmJ [H]

Identifier: 134301622

GI number: 134301622

Start: 557633

End: 558376

Strand: Direct

Name: trmJ [H]

Synonym: FTW_0561

Alternate gene names: 134301622

Gene position: 557633-558376 (Clockwise)

Preceding gene: 134301621

Following gene: 134301623

Centisome position: 29.37

GC content: 35.08

Gene sequence:

>744_bases
ATGAATAATCTATTTAATAATATACGTATAGTTCTAGTAGAACCATCACACAGTGGTAATGTTGGTTCTACTGCTAGAGC
AATGCTTAATATGGGGTTGACAAACTTATGGCTTGTAAACCCTAAAAAAGGTATTGATGATGAGGCATTAGCTCTATCAT
GTCATGCTACAGAAGTAGTAAATAATGCTACTATTGTAGCTAATCTAGAAGAGGCTCTTGAAGGTGTCGATTATGTGGTT
GGAACTAGCGCAAGAGTGCGTAGAGTATCTCTACCTATAGAGCCTATATCTAAAGTTGCTACAAGCATACTTAATAAAAT
CCAAAAATCTGATGAAAAAATAGCAATATTATTTGGCAGAGAAAGAACGGGACTTTTAAATGAAGAGCTTTTGATGAGTA
ATGTACATGCGTATATCCCGTCAAATGAAGAATATACATCTCTTAATCTAGCTCAGGCTGTACAACTAGTAGCTTATGAG
ATTTATAAGCAAGCAATTGAGATTAGCAAGCTTAAACAAGTTCCTGAGTACAATCATTTACATAAAAAAGCTTCCGTAAA
AGAGCTGCAAGGTCTATATCAGCATTTTGAGGATAGTATGATCAAGTCGGGATTCTTAGATAAAGATAAACCTGGGCATG
TCATGGATAAAGTGCGCAGACTTTTTCAAAGATCCGAACTTGAGAGTCAAGAAGTGAATATTTTGAGAGGTTTTTTAACC
TCATTAGAAAATCAGGATAAGTAG

Upstream 100 bases:

>100_bases
AAGCAATTAGCTTTATATTTGAAACAAGATAAAACGCAGCAGTATTTTCAGCAAGCAGAAAATTCCGAAGATTTGTATAA
TTTAATTATTAATACTAGTA

Downstream 100 bases:

>100_bases
TATGATTATACTAGGTATAGATGAAGCAGGGCGTGGACCATTATCAGGGCCTGTAGTTGCTGCAGGAGTTATATTGGATC
AAGATAAAATTATTGATGGT

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MNNLFNNIRIVLVEPSHSGNVGSTARAMLNMGLTNLWLVNPKKGIDDEALALSCHATEVVNNATIVANLEEALEGVDYVV
GTSARVRRVSLPIEPISKVATSILNKIQKSDEKIAILFGRERTGLLNEELLMSNVHAYIPSNEEYTSLNLAQAVQLVAYE
IYKQAIEISKLKQVPEYNHLHKKASVKELQGLYQHFEDSMIKSGFLDKDKPGHVMDKVRRLFQRSELESQEVNILRGFLT
SLENQDK

Sequences:

>Translated_247_residues
MNNLFNNIRIVLVEPSHSGNVGSTARAMLNMGLTNLWLVNPKKGIDDEALALSCHATEVVNNATIVANLEEALEGVDYVV
GTSARVRRVSLPIEPISKVATSILNKIQKSDEKIAILFGRERTGLLNEELLMSNVHAYIPSNEEYTSLNLAQAVQLVAYE
IYKQAIEISKLKQVPEYNHLHKKASVKELQGLYQHFEDSMIKSGFLDKDKPGHVMDKVRRLFQRSELESQEVNILRGFLT
SLENQDK
>Mature_247_residues
MNNLFNNIRIVLVEPSHSGNVGSTARAMLNMGLTNLWLVNPKKGIDDEALALSCHATEVVNNATIVANLEEALEGVDYVV
GTSARVRRVSLPIEPISKVATSILNKIQKSDEKIAILFGRERTGLLNEELLMSNVHAYIPSNEEYTSLNLAQAVQLVAYE
IYKQAIEISKLKQVPEYNHLHKKASVKELQGLYQHFEDSMIKSGFLDKDKPGHVMDKVRRLFQRSELESQEVNILRGFLT
SLENQDK

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=244, Percent_Identity=46.7213114754098, Blast_Score=217, Evalue=7e-58,
Organism=Escherichia coli, GI1790865, Length=158, Percent_Identity=32.2784810126582, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27727; Mature: 27727

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLFNNIRIVLVEPSHSGNVGSTARAMLNMGLTNLWLVNPKKGIDDEALALSCHATEVV
CCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHEEEEHHHHHHH
NNATIVANLEEALEGVDYVVGTSARVRRVSLPIEPISKVATSILNKIQKSDEKIAILFGR
CCCCEEEEHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECC
ERTGLLNEELLMSNVHAYIPSNEEYTSLNLAQAVQLVAYEIYKQAIEISKLKQVPEYNHL
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
HKKASVKELQGLYQHFEDSMIKSGFLDKDKPGHVMDKVRRLFQRSELESQEVNILRGFLT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SLENQDK
HHCCCCC
>Mature Secondary Structure
MNNLFNNIRIVLVEPSHSGNVGSTARAMLNMGLTNLWLVNPKKGIDDEALALSCHATEVV
CCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHEEEEHHHHHHH
NNATIVANLEEALEGVDYVVGTSARVRRVSLPIEPISKVATSILNKIQKSDEKIAILFGR
CCCCEEEEHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECC
ERTGLLNEELLMSNVHAYIPSNEEYTSLNLAQAVQLVAYEIYKQAIEISKLKQVPEYNHL
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
HKKASVKELQGLYQHFEDSMIKSGFLDKDKPGHVMDKVRRLFQRSELESQEVNILRGFLT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SLENQDK
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA