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The map label for this gene is truB [H]

Identifier: 134301464

GI number: 134301464

Start: 367139

End: 368047

Strand: Direct

Name: truB [H]

Synonym: FTW_0373

Alternate gene names: 134301464

Gene position: 367139-368047 (Clockwise)

Preceding gene: 134301463

Following gene: 134301465

Centisome position: 19.34

GC content: 31.79

Gene sequence:

>909_bases
ATGAAAAAAAATAGACTAAACTTAAATGGTGTGGTTGTTATTAATAAGGCTAAAGATATTAGTTCAAATAAAGTTTTACA
GCAATTAAAGTATTTATTTAATGCACAAAAAGTAGGTCATACAGGTACTTTGGATCCTATGGCGACTGGAGTTCTACCGA
TATGTTTTGGTAGGGCTACAAAAATAGCACAATACTTACTTGATGCTGATAAAGAGTATATAGCTACAATTAGACTCGGT
ATTGAAACTGATAGTGGTGATGCTGAGGGTGAAATAATAGCAAAAAGCATTAACATTCCTGAGTTATCTGCTGAGTATCT
AGAAACAGTTTTAGCAAAGTTTCGCGGAGATGTAGTCCAAATCCCTCCAATGTACTCAGCTCTTAAATATAATGGACAAC
CTCTTTATAAGTTAGCAAGGGAAGGTAAAACTGTTGAGGTGAAGTCACGCAATATCAAAATATATGAGTTAGAACTACTA
GAGTTTAATATAGATTCATTAAAAATAAGAGTTAAATGTTCAAAAGGAACATATATCCGTAGTTTAGCTATAGATATTGG
AAAGACTCTAGGTTGTGGAGGGCACTTAATAGCTTTACAAAGAACTCAAAGTGGACCTTTTAAATTATCTGAGGCTTTTA
GGCTAGAACAACTAAAAGATTTAAGTTTTGAGCAAAAAATTGCTAGTATAACTAATATAGAGAGTGTATTTATCGACAAA
CCAATATATTCTTTATTAGAAGAAGAAAAAGATGATTTATATAAAAGAGGGCTCTTTGCTGACAAACCTCATCTTGATGG
AACGGTCAGGATATATGATGTTGAGAAGTTTGTTGCTATAGCAGAGTTTGATAAAGGTAAGTTGATTAATAAAAAATTTT
TTGATCAGGATATATTGATAAGTGAGTAA

Upstream 100 bases:

>100_bases
GATTTAAATCTTCGAGTTAAAGCTCAGGGCACTTCACGGAAAAAAGCTGAGCAAAAAACTGCTGAAAAAATGATAGAAAT
GTTATCACAACAAGGCTTAC

Downstream 100 bases:

>100_bases
GTACAGCATACATAATGATCCGAATAAGGATATTGAAGCGCAAAAATATGAAAATCCAATTCCAAGTAGGGAAGTGATTT
TAAATTATATAAGAGATGTA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKKNRLNLNGVVVINKAKDISSNKVLQQLKYLFNAQKVGHTGTLDPMATGVLPICFGRATKIAQYLLDADKEYIATIRLG
IETDSGDAEGEIIAKSINIPELSAEYLETVLAKFRGDVVQIPPMYSALKYNGQPLYKLAREGKTVEVKSRNIKIYELELL
EFNIDSLKIRVKCSKGTYIRSLAIDIGKTLGCGGHLIALQRTQSGPFKLSEAFRLEQLKDLSFEQKIASITNIESVFIDK
PIYSLLEEEKDDLYKRGLFADKPHLDGTVRIYDVEKFVAIAEFDKGKLINKKFFDQDILISE

Sequences:

>Translated_302_residues
MKKNRLNLNGVVVINKAKDISSNKVLQQLKYLFNAQKVGHTGTLDPMATGVLPICFGRATKIAQYLLDADKEYIATIRLG
IETDSGDAEGEIIAKSINIPELSAEYLETVLAKFRGDVVQIPPMYSALKYNGQPLYKLAREGKTVEVKSRNIKIYELELL
EFNIDSLKIRVKCSKGTYIRSLAIDIGKTLGCGGHLIALQRTQSGPFKLSEAFRLEQLKDLSFEQKIASITNIESVFIDK
PIYSLLEEEKDDLYKRGLFADKPHLDGTVRIYDVEKFVAIAEFDKGKLINKKFFDQDILISE
>Mature_302_residues
MKKNRLNLNGVVVINKAKDISSNKVLQQLKYLFNAQKVGHTGTLDPMATGVLPICFGRATKIAQYLLDADKEYIATIRLG
IETDSGDAEGEIIAKSINIPELSAEYLETVLAKFRGDVVQIPPMYSALKYNGQPLYKLAREGKTVEVKSRNIKIYELELL
EFNIDSLKIRVKCSKGTYIRSLAIDIGKTLGCGGHLIALQRTQSGPFKLSEAFRLEQLKDLSFEQKIASITNIESVFIDK
PIYSLLEEEKDDLYKRGLFADKPHLDGTVRIYDVEKFVAIAEFDKGKLINKKFFDQDILISE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=223, Percent_Identity=36.7713004484305, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI4503337, Length=199, Percent_Identity=29.6482412060301, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI215599015, Length=199, Percent_Identity=29.6482412060301, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI2367200, Length=302, Percent_Identity=39.4039735099338, Blast_Score=214, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI17553978, Length=246, Percent_Identity=26.4227642276423, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=135, Percent_Identity=44.4444444444444, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=242, Percent_Identity=31.8181818181818, Blast_Score=102, Evalue=8e-23,
Organism=Drosophila melanogaster, GI281364189, Length=199, Percent_Identity=29.6482412060301, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281364187, Length=199, Percent_Identity=29.6482412060301, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281364185, Length=199, Percent_Identity=29.6482412060301, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281364183, Length=199, Percent_Identity=29.6482412060301, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI62471759, Length=199, Percent_Identity=29.6482412060301, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI17975520, Length=199, Percent_Identity=29.6482412060301, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33959; Mature: 33959

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNRLNLNGVVVINKAKDISSNKVLQQLKYLFNAQKVGHTGTLDPMATGVLPICFGRAT
CCCCEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHCHHH
KIAQYLLDADKEYIATIRLGIETDSGDAEGEIIAKSINIPELSAEYLETVLAKFRGDVVQ
HHHHHHHHCCCCEEEEEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEE
IPPMYSALKYNGQPLYKLAREGKTVEVKSRNIKIYELELLEFNIDSLKIRVKCSKGTYIR
CCCCHHHHHCCCCHHHHHHHCCCEEEEECCCEEEEEEEEEEECCCEEEEEEEECCCCEEE
SLAIDIGKTLGCGGHLIALQRTQSGPFKLSEAFRLEQLKDLSFEQKIASITNIESVFIDK
HHHHHHCCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
PIYSLLEEEKDDLYKRGLFADKPHLDGTVRIYDVEKFVAIAEFDKGKLINKKFFDQDILI
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHEEEEEECCCCCCCCHHHCCCCCEE
SE
CC
>Mature Secondary Structure
MKKNRLNLNGVVVINKAKDISSNKVLQQLKYLFNAQKVGHTGTLDPMATGVLPICFGRAT
CCCCEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHCHHH
KIAQYLLDADKEYIATIRLGIETDSGDAEGEIIAKSINIPELSAEYLETVLAKFRGDVVQ
HHHHHHHHCCCCEEEEEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEE
IPPMYSALKYNGQPLYKLAREGKTVEVKSRNIKIYELELLEFNIDSLKIRVKCSKGTYIR
CCCCHHHHHCCCCHHHHHHHCCCEEEEECCCEEEEEEEEEEECCCEEEEEEEECCCCEEE
SLAIDIGKTLGCGGHLIALQRTQSGPFKLSEAFRLEQLKDLSFEQKIASITNIESVFIDK
HHHHHHCCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
PIYSLLEEEKDDLYKRGLFADKPHLDGTVRIYDVEKFVAIAEFDKGKLINKKFFDQDILI
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHEEEEEECCCCCCCCHHHCCCCCEE
SE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA