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The map label for this gene is gltA [H]

Identifier: 134301285

GI number: 134301285

Start: 157199

End: 158464

Strand: Reverse

Name: gltA [H]

Synonym: FTW_0147

Alternate gene names: 134301285

Gene position: 158464-157199 (Counterclockwise)

Preceding gene: 134301299

Following gene: 134301284

Centisome position: 8.35

GC content: 36.33

Gene sequence:

>1266_bases
ATGTTAATGAGTAAATACGCAACTCTTAAGTATGCAGATAAAAATATTGAAATAGAATTACCAGTATATTCTCCTAGCTT
GGGTAATGACTGCATAGATGTTTCATCATTAGTAAAACACGGAATTTTTACTTATGATCCTGGATTCATGTCTACAGCTG
CATGCGAATCTAAAATTACATATATCGATGGTGGTAAAGGTGTACTCTTACATAGAGGCTACCCAATCGAAGAATGGACT
CAAAAATCAAACTACAGAACTCTTTGCTATGCATTGATTTATGGCGAACTCCCAACAGATGAACAGGTTAAAAGCTTTAG
ACAAGAAATTATCAATAAAATGCCAGTATGCGAGCATGTCAAAGCTGCAATAGCTGCAATGCCTCAACATACACATCCAA
TGTCAAGCTTAATAGCTGGTGTTAATGTCTTAGCTGCTGAACATATCCACAATGGACAAAAAGAATCTCAAGATGAAGTC
GCAAAAAATATCGTAGCAAAAATTGCAACTATAGCAGCCATGGCTTACAGACATAATCACGGCAAGAAATTCTTAGAACC
TAAAATGGAATATGGCTATGCTGAGAATTTCTTGTATATGATGTTTGCTGATGATGAGAGCTACAAACCAGATGAGCTAC
ATATCAAAGCAATGGATACAATATTTATGCTTCATGCTGATCACGAGCAAAATGCTTCAACATCAACTGTAAGGTTATCA
GGATCAACTGGTAACTCTCCTTATGCGGCAATTATCGCAGGTATTACCGCATTATGGGGACCTGCTCATGGCGGCGCTAA
TGAAGCTGTGCTTAAAATGTTATCTGAAATTGGTAGCACTGAGAATATCGATAAGTATATTGCTAAAGCGAAAGATAAAG
ATGATCCATTTAGATTAATGGGCTTTGGACATAGAGTCTACAAAAATACTGATCCGAGAGCTACTGCAATGAAAAAGAAC
TGTGAAGAAATACTTGCTAAACTTGGTCATAGTGATAACCCTCTTCTCACAGTAGCTAAAAAACTAGAAGAAATTGCTTT
ACAAGATGAGTTCTTTATCGAGAGAAAGCTTTTCTCTAATGTTGATTTTTACTCAGGTATAATCTTAAAAGCCATTGGCA
TTCCAGAAGATATGTTTACAGCCATATTTGCTCTAGCAAGAACATCAGGGTGGATATCTCAATGGATAGAGATGGTTAAT
GATCCTGCTCAAAAGATTGGTCGTCCAAGACAATTATATACTGGTGCAACAAATAGAAATTTCTAA

Upstream 100 bases:

>100_bases
TTGTAATGATACCTTATAACTATTTTCTTTTTTGACGAAAGGTATCTTTTTACTGCAAAACCTTGCATTATAGTTTCGAT
CAACTCTTCACTTGGAGGTC

Downstream 100 bases:

>100_bases
TTTTATTTTCTTTGCTCTATTCTTTATTTACTTTCTTAGTTATTTCAACTAGTATTTTATCTATATAAAAAATCCATATA
GATAAGAAATGACTAGTTCT

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MLMSKYATLKYADKNIEIELPVYSPSLGNDCIDVSSLVKHGIFTYDPGFMSTAACESKITYIDGGKGVLLHRGYPIEEWT
QKSNYRTLCYALIYGELPTDEQVKSFRQEIINKMPVCEHVKAAIAAMPQHTHPMSSLIAGVNVLAAEHIHNGQKESQDEV
AKNIVAKIATIAAMAYRHNHGKKFLEPKMEYGYAENFLYMMFADDESYKPDELHIKAMDTIFMLHADHEQNASTSTVRLS
GSTGNSPYAAIIAGITALWGPAHGGANEAVLKMLSEIGSTENIDKYIAKAKDKDDPFRLMGFGHRVYKNTDPRATAMKKN
CEEILAKLGHSDNPLLTVAKKLEEIALQDEFFIERKLFSNVDFYSGIILKAIGIPEDMFTAIFALARTSGWISQWIEMVN
DPAQKIGRPRQLYTGATNRNF

Sequences:

>Translated_421_residues
MLMSKYATLKYADKNIEIELPVYSPSLGNDCIDVSSLVKHGIFTYDPGFMSTAACESKITYIDGGKGVLLHRGYPIEEWT
QKSNYRTLCYALIYGELPTDEQVKSFRQEIINKMPVCEHVKAAIAAMPQHTHPMSSLIAGVNVLAAEHIHNGQKESQDEV
AKNIVAKIATIAAMAYRHNHGKKFLEPKMEYGYAENFLYMMFADDESYKPDELHIKAMDTIFMLHADHEQNASTSTVRLS
GSTGNSPYAAIIAGITALWGPAHGGANEAVLKMLSEIGSTENIDKYIAKAKDKDDPFRLMGFGHRVYKNTDPRATAMKKN
CEEILAKLGHSDNPLLTVAKKLEEIALQDEFFIERKLFSNVDFYSGIILKAIGIPEDMFTAIFALARTSGWISQWIEMVN
DPAQKIGRPRQLYTGATNRNF
>Mature_421_residues
MLMSKYATLKYADKNIEIELPVYSPSLGNDCIDVSSLVKHGIFTYDPGFMSTAACESKITYIDGGKGVLLHRGYPIEEWT
QKSNYRTLCYALIYGELPTDEQVKSFRQEIINKMPVCEHVKAAIAAMPQHTHPMSSLIAGVNVLAAEHIHNGQKESQDEV
AKNIVAKIATIAAMAYRHNHGKKFLEPKMEYGYAENFLYMMFADDESYKPDELHIKAMDTIFMLHADHEQNASTSTVRLS
GSTGNSPYAAIIAGITALWGPAHGGANEAVLKMLSEIGSTENIDKYIAKAKDKDDPFRLMGFGHRVYKNTDPRATAMKKN
CEEILAKLGHSDNPLLTVAKKLEEIALQDEFFIERKLFSNVDFYSGIILKAIGIPEDMFTAIFALARTSGWISQWIEMVN
DPAQKIGRPRQLYTGATNRNF

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=356, Percent_Identity=25.8426966292135, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786939, Length=408, Percent_Identity=52.2058823529412, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1786527, Length=366, Percent_Identity=29.5081967213115, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17555174, Length=383, Percent_Identity=26.1096605744125, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324328, Length=373, Percent_Identity=29.2225201072386, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319850, Length=352, Percent_Identity=28.4090909090909, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6325257, Length=362, Percent_Identity=27.3480662983425, Blast_Score=105, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24640124, Length=400, Percent_Identity=27, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24640126, Length=400, Percent_Identity=27, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21356863, Length=374, Percent_Identity=26.2032085561497, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47021; Mature: 47021

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMSKYATLKYADKNIEIELPVYSPSLGNDCIDVSSLVKHGIFTYDPGFMSTAACESKIT
CCCCCCCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHCCEEE
YIDGGKGVLLHRGYPIEEWTQKSNYRTLCYALIYGELPTDEQVKSFRQEIINKMPVCEHV
EEECCCEEEEECCCCHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHH
KAAIAAMPQHTHPMSSLIAGVNVLAAEHIHNGQKESQDEVAKNIVAKIATIAAMAYRHNH
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GKKFLEPKMEYGYAENFLYMMFADDESYKPDELHIKAMDTIFMLHADHEQNASTSTVRLS
CHHHHCHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCEEEEE
GSTGNSPYAAIIAGITALWGPAHGGANEAVLKMLSEIGSTENIDKYIAKAKDKDDPFRLM
CCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHH
GFGHRVYKNTDPRATAMKKNCEEILAKLGHSDNPLLTVAKKLEEIALQDEFFIERKLFSN
CCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDFYSGIILKAIGIPEDMFTAIFALARTSGWISQWIEMVNDPAQKIGRPRQLYTGATNRN
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCCC
F
C
>Mature Secondary Structure
MLMSKYATLKYADKNIEIELPVYSPSLGNDCIDVSSLVKHGIFTYDPGFMSTAACESKIT
CCCCCCCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHCCEEE
YIDGGKGVLLHRGYPIEEWTQKSNYRTLCYALIYGELPTDEQVKSFRQEIINKMPVCEHV
EEECCCEEEEECCCCHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHH
KAAIAAMPQHTHPMSSLIAGVNVLAAEHIHNGQKESQDEVAKNIVAKIATIAAMAYRHNH
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GKKFLEPKMEYGYAENFLYMMFADDESYKPDELHIKAMDTIFMLHADHEQNASTSTVRLS
CHHHHCHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCEEEEE
GSTGNSPYAAIIAGITALWGPAHGGANEAVLKMLSEIGSTENIDKYIAKAKDKDDPFRLM
CCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHH
GFGHRVYKNTDPRATAMKKNCEEILAKLGHSDNPLLTVAKKLEEIALQDEFFIERKLFSN
CCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDFYSGIILKAIGIPEDMFTAIFALARTSGWISQWIEMVNDPAQKIGRPRQLYTGATNRN
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2507528; 10984043 [H]