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The map label for this gene is nuoM [H]

Identifier: 134301258

GI number: 134301258

Start: 127483

End: 129072

Strand: Direct

Name: nuoM [H]

Synonym: FTW_0118

Alternate gene names: 134301258

Gene position: 127483-129072 (Clockwise)

Preceding gene: 134301257

Following gene: 134301259

Centisome position: 6.72

GC content: 36.29

Gene sequence:

>1590_bases
ATGAATTTAGGTAATTACTTATTAAGTTTAATAATCTGGCTCCCAATAGTTGGTGGTTTTGTTGTTTTGGCGACAAGAAC
CAAAGAGTTGCATGGCGATGCAGCACGTTGGGTAGCATTAGTCTTTAGTTGTTTAACTCTAGCTTTATGTGTTCCGTTAG
TTACATCTTTTGATTATAGTAGTTCAGCAATGCAGTTCCAAGAATCAGTAAAATGGTTTAAGGTTTTTGGCATGCATGAT
ATTTACTATAGTTTGGGAGTAGATGGATTTTCTGTATTATTTATAGTTTTGACATCTTTTGCTACATTAGTGATAGTTTT
AGCTGCTTGGACATCTATCAAAACTAAAGTTAGACAATACATGGCGATATTTTTGATTACATGTGGTTTGACTAATGGCG
TTTTCTGTGCGACTGATTCGATACTTTATTATGTTTTCTGGGAGGCGCTATTAATTCCAACTTGCGTTGGTATTGGTATT
TGGGGTGGTAAGCACAAAGCGTATGCTGCAGTTAAATACTTTATGTATACATTCTTTGGTTCGGTATTTTTACTAGCAGC
TATTTTGTATATTCAAACACGAGTTGCTGCTTCACCGACACACTTTTTGGTAAATCAAGATACTTATTCTATTCAGAATT
TTATTGCTTGGGCAACTCAGTCACAAGGCTTTATAGATTCTGTTAAATTTACTCTAACAGCACAATGGTTAGTTTTTGGG
GCATTTTTCTTAGCATTTGCTGTTAAGATTCCGATGTGGCCATTTCACTCATGGTTACCAGATGCTCACTCAGAGGCGCC
TGCTGGTGGTTCTGTCATCCTTGCAGCCCTTATGCTTAAGCTAGGTGCTTATGGTTTCTTAAGATTTGCTATTCCAATGC
TGCCAGAGGTAACAGCATCTCTCGAGTATGTTTTGATTATAATGTCTTTGATAGCTATAGTTTATGTTGGGGTTGTTGCT
GTTGCACAAACTGATGTTAAAAGACTAATTGCATATTCATCAATTTCTCATATGGGTCTAGTTACTCTTGGTTTATTCTC
AATATTTATATTGAAAAATGCTGATCCAGTATTAGGGACAACTCATGCGCAATTAGCTTTACAAGGCGCAGTGTTTCAGA
TGATTGCTCATGCTTTTTCATCTGGTGGTATGTTTATTGGTATTGGTTATTTATATTTGAGAATGCATACTAGAGAAATA
TCAGATTTCTCTGGTGTAGCTAAAACTATGCCAATATTTGCAACATTCTTTTTGCTTTTCTGTATGGCGAATGTTGGTTT
ACCTGGCACAAGTGGATTTGTTGGTGAGTTTATGATCTTACTGGCAGTATTTCAATACTCTCCATTAATAGCATTAATTG
CAGGATTAACTCTTGTGATCGCTCCAATTTATACTCTTTGGATGTATAAGCGAGTTTTCTTCGGTGAGGTTGTATCTACA
CAGGTAGCAAGCTTGACTGATTTAAATAGGATGGAGCTATTTGTATTTATATTATTAGCGGTACCGACTTTATTGTTTGG
TTTCTATCCAGAGCCAATTTTGCAGCTATCAGCAGCAGCATCGGCACATATTGTTGGTCTATCTCTATAA

Upstream 100 bases:

>100_bases
GGATATTTGTTTGACTATGCATTTGTGATGATGGTTGGTGTATTATTGTTTATGATTTTGCTAATTTCTGTTTAGGCAAG
TTAGAGGATAAATTTGTATT

Downstream 100 bases:

>100_bases
GGTGGTGTTTTGATTATGAGTTTATCAATCCTTTATATTTTGCCAGAAATACTACTAGCACTAGGTGTCATAGTTGTAAT
GTTTTCTGGACTTTTTCTAC

Product: NADH dehydrogenase I, M subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MNLGNYLLSLIIWLPIVGGFVVLATRTKELHGDAARWVALVFSCLTLALCVPLVTSFDYSSSAMQFQESVKWFKVFGMHD
IYYSLGVDGFSVLFIVLTSFATLVIVLAAWTSIKTKVRQYMAIFLITCGLTNGVFCATDSILYYVFWEALLIPTCVGIGI
WGGKHKAYAAVKYFMYTFFGSVFLLAAILYIQTRVAASPTHFLVNQDTYSIQNFIAWATQSQGFIDSVKFTLTAQWLVFG
AFFLAFAVKIPMWPFHSWLPDAHSEAPAGGSVILAALMLKLGAYGFLRFAIPMLPEVTASLEYVLIIMSLIAIVYVGVVA
VAQTDVKRLIAYSSISHMGLVTLGLFSIFILKNADPVLGTTHAQLALQGAVFQMIAHAFSSGGMFIGIGYLYLRMHTREI
SDFSGVAKTMPIFATFFLLFCMANVGLPGTSGFVGEFMILLAVFQYSPLIALIAGLTLVIAPIYTLWMYKRVFFGEVVST
QVASLTDLNRMELFVFILLAVPTLLFGFYPEPILQLSAAASAHIVGLSL

Sequences:

>Translated_529_residues
MNLGNYLLSLIIWLPIVGGFVVLATRTKELHGDAARWVALVFSCLTLALCVPLVTSFDYSSSAMQFQESVKWFKVFGMHD
IYYSLGVDGFSVLFIVLTSFATLVIVLAAWTSIKTKVRQYMAIFLITCGLTNGVFCATDSILYYVFWEALLIPTCVGIGI
WGGKHKAYAAVKYFMYTFFGSVFLLAAILYIQTRVAASPTHFLVNQDTYSIQNFIAWATQSQGFIDSVKFTLTAQWLVFG
AFFLAFAVKIPMWPFHSWLPDAHSEAPAGGSVILAALMLKLGAYGFLRFAIPMLPEVTASLEYVLIIMSLIAIVYVGVVA
VAQTDVKRLIAYSSISHMGLVTLGLFSIFILKNADPVLGTTHAQLALQGAVFQMIAHAFSSGGMFIGIGYLYLRMHTREI
SDFSGVAKTMPIFATFFLLFCMANVGLPGTSGFVGEFMILLAVFQYSPLIALIAGLTLVIAPIYTLWMYKRVFFGEVVST
QVASLTDLNRMELFVFILLAVPTLLFGFYPEPILQLSAAASAHIVGLSL
>Mature_529_residues
MNLGNYLLSLIIWLPIVGGFVVLATRTKELHGDAARWVALVFSCLTLALCVPLVTSFDYSSSAMQFQESVKWFKVFGMHD
IYYSLGVDGFSVLFIVLTSFATLVIVLAAWTSIKTKVRQYMAIFLITCGLTNGVFCATDSILYYVFWEALLIPTCVGIGI
WGGKHKAYAAVKYFMYTFFGSVFLLAAILYIQTRVAASPTHFLVNQDTYSIQNFIAWATQSQGFIDSVKFTLTAQWLVFG
AFFLAFAVKIPMWPFHSWLPDAHSEAPAGGSVILAALMLKLGAYGFLRFAIPMLPEVTASLEYVLIIMSLIAIVYVGVVA
VAQTDVKRLIAYSSISHMGLVTLGLFSIFILKNADPVLGTTHAQLALQGAVFQMIAHAFSSGGMFIGIGYLYLRMHTREI
SDFSGVAKTMPIFATFFLLFCMANVGLPGTSGFVGEFMILLAVFQYSPLIALIAGLTLVIAPIYTLWMYKRVFFGEVVST
QVASLTDLNRMELFVFILLAVPTLLFGFYPEPILQLSAAASAHIVGLSL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=386, Percent_Identity=32.3834196891192, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI251831117, Length=446, Percent_Identity=22.8699551569507, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788613, Length=531, Percent_Identity=37.8531073446328, Blast_Score=299, Evalue=4e-82,
Organism=Escherichia coli, GI1788827, Length=277, Percent_Identity=29.9638989169675, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788831, Length=391, Percent_Identity=25.5754475703325, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788829, Length=513, Percent_Identity=26.5107212475634, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788614, Length=492, Percent_Identity=25.4065040650407, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693160, Length=377, Percent_Identity=23.6074270557029, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI2367154, Length=399, Percent_Identity=24.0601503759398, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 58151; Mature: 58151

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHEEEECC
>Mature Secondary Structure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HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]