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The map label for this gene is nuoB [H]

Identifier: 134301247

GI number: 134301247

Start: 116443

End: 116919

Strand: Direct

Name: nuoB [H]

Synonym: FTW_0107

Alternate gene names: 134301247

Gene position: 116443-116919 (Clockwise)

Preceding gene: 134301246

Following gene: 134301248

Centisome position: 6.13

GC content: 43.61

Gene sequence:

>477_bases
GTGGGAATAGGTAACGAAAACAAAGGTTTTATAACTGCAAGTGCGGATGCACTTATAAACTGGGTGCGTACAGGATCTTT
ATGGCCGGTAACAACTGGTTTGGCTTGTTGTGCTGTAGAAATGATGCACGCAGGTGCGGCTAGGTATGATCTGGATAGGT
TTGGTATAGTCTTTAGGCCTTCTCCAAGGCAGTCTGATGTGCTTATTGTTGCTGGGACTCTTTGCAATAAAATGGCTCCG
GCGCTACGCCAAGTGTATGATCAAATGCCTGACCCTAAGTGGGTAATTTCTATGGGATCTTGTGCAAATGGTGGTGGTTA
TTATCATTACTCGTACTCGGTGGTTAGGGGTTGTGATAGGATTGTGCCTGTTGATATATATGTGCCAGGTTGTCCTCCTA
CTGCTGAGGCTTTAGTTTACGGCATTATACAACTGCAGAATAAAATTATTAGAAAAGATACTATAGCGAGGAAGTGA

Upstream 100 bases:

>100_bases
GAATGCCAGCTATTTCAGATCATGCATTTTTTGCGATGATTATATTTTTAGTGGTGTTATTCTTGGGCTTAATATACGCC
TGGAAGAAAGGAGCTTTAGA

Downstream 100 bases:

>100_bases
TCGTGAGTACTAAATTACAAGATCATTTTGATAAAATAACAAAGATTTTGAGTGGCTTTGGCGTTGAGGGCTGTATATCT
TATGGCGAGATAACTTTTTC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MGIGNENKGFITASADALINWVRTGSLWPVTTGLACCAVEMMHAGAARYDLDRFGIVFRPSPRQSDVLIVAGTLCNKMAP
ALRQVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALVYGIIQLQNKIIRKDTIARK

Sequences:

>Translated_158_residues
MGIGNENKGFITASADALINWVRTGSLWPVTTGLACCAVEMMHAGAARYDLDRFGIVFRPSPRQSDVLIVAGTLCNKMAP
ALRQVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALVYGIIQLQNKIIRKDTIARK
>Mature_157_residues
GIGNENKGFITASADALINWVRTGSLWPVTTGLACCAVEMMHAGAARYDLDRFGIVFRPSPRQSDVLIVAGTLCNKMAPA
LRQVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALVYGIIQLQNKIIRKDTIARK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=76.7123287671233, Blast_Score=250, Evalue=4e-67,
Organism=Escherichia coli, GI1788624, Length=144, Percent_Identity=50.6944444444444, Blast_Score=158, Evalue=1e-40,
Organism=Escherichia coli, GI1789074, Length=107, Percent_Identity=46.7289719626168, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=42.6229508196721, Blast_Score=96, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=143, Percent_Identity=71.3286713286713, Blast_Score=235, Evalue=9e-63,
Organism=Drosophila melanogaster, GI18859983, Length=143, Percent_Identity=77.6223776223776, Blast_Score=250, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24642371, Length=143, Percent_Identity=77.6223776223776, Blast_Score=250, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=77.3972602739726, Blast_Score=247, Evalue=3e-66,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17258; Mature: 17127

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIGNENKGFITASADALINWVRTGSLWPVTTGLACCAVEMMHAGAARYDLDRFGIVFRP
CCCCCCCCEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCEEECC
SPRQSDVLIVAGTLCNKMAPALRQVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDR
CCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCC
IVPVDIYVPGCPPTAEALVYGIIQLQNKIIRKDTIARK
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GIGNENKGFITASADALINWVRTGSLWPVTTGLACCAVEMMHAGAARYDLDRFGIVFRP
CCCCCCCEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCEEECC
SPRQSDVLIVAGTLCNKMAPALRQVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDR
CCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCC
IVPVDIYVPGCPPTAEALVYGIIQLQNKIIRKDTIARK
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA