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The map label for this gene is gatB

Identifier: 134301238

GI number: 134301238

Start: 104515

End: 105936

Strand: Direct

Name: gatB

Synonym: FTW_0095

Alternate gene names: 134301238

Gene position: 104515-105936 (Clockwise)

Preceding gene: 134301237

Following gene: 134301239

Centisome position: 5.51

GC content: 36.92

Gene sequence:

>1422_bases
GTGAATTGGGAAATGGTGATAGGGCTAGAAGTCCATATTCAATTAAGTACTAAATCTAAGCTATTTTCGACTTCTGCAAC
AAAGTATGGTCAACATCAAAATACTCAAGCAGCATTTTTAGACTTAGGTTTGCCTGGAACGTTGCCTGTTGTAAATAAAG
AAGCTATTCGTAAAGCAGTGATATTTGGCTTAGCTGTAGATGCTAAGATATCAAAGGACAGTTTCTTTGCGCGAAAAAAT
TATTTCTATCCAGATTTATCAAAAGGTTATCAGATTAGTCAATCAACTAATCCAATAGTCCAAGAAGGTAGATTAGAGAT
TGAAACTTCAAAAGGACTAAAAACTATCAGAATTGAGCGTGCACATTTAGAAGAAGATGCCGGTAAGTCTGTGCATGGTT
ATATTGCTGGTGAGACAGGATTAGATTACAATCGTGCTGGTACGCCACTTTTAGAGATTGTGACATATCCTGATTTTAGA
TCGGCTGAAGAAGTGGTTGCATATCTTAAAAAACTTCATCAGCTAGTTAAGCATTTAGGTATTTGCGATGGTAACATGCA
AGAAGGTTCGTTTAGATGTGATGTTAACTTGTCAATTAGACCACAAGGACAAGCTAAGTTTGGTACACGTGCAGAGCTTA
AAAATATAAACTCATTTAGGTTTATTGATAAAGCTATCGAGTATGAGTATGCTCGCCAAGTTAGTGTGTTGGAGTCAGGG
GGCGAAGTTGTCCAGGAAACGCGTCTTTATGATGCAGATGCTAATGAGACGCGCTCAATGCGTGCAAAAGAAGATGCTTT
TGATTATCGTTATTTCCCAGATCCAGATTTATTGCCATTGGTGATAACCGATGAGTATATCGAGAGTATCAAAAAACAAA
TGCCACTGAAATCGGAAGAGCGTGAAGCGGTATATCGTGAGCATCTAGCTGAGCAGGAAGTTGAGTTTTTATTATCAAAT
CTTGAGATAGCTGATTACTATGACAAAGTAGCAGTTGTGATAGGATACAAGCCAGCTTATAATTGGATAACAGTTGATCT
AATATCAACTCTTAATAGGGCTGAAAAGGAGTTTTCTAGTGATGTTGTTCCTGCTGAGATTCTGCTAGAAATTATTGCTA
ATGTCCAAAAAGATATAATATCTCAAGCTAATGCTAAGAAAGTGATAGCAGAATATATTGATGCTCCTAGTGCAATTGAA
GCTATTATTGAAAAGCTCGGTCTAAAACAAGTATCTGATGAGGGTATGATTCGCGAGTTAGTTCAAGGTATAATTGCTGC
TAACCCTCAACAAGCAGCTGACTTTAAAGCTGGTAAAACAAAGCTTATGAGCTTTTTTGTTGGGCAAGCTATGAAAGCAA
GTAAAGGTAAAGCAAATCCTAAGCAGGTTAATCAAATAGTTCAAGAAGAGCTAAATAAATAA

Upstream 100 bases:

>100_bases
CTAAAGCATTTAATGATAATATCTTGACGCAGATGGTAGTGCAGTATCAAAAGCATTATGGTATTGAAGAATTTATTTTA
CAACAAGCGAGGATTTAATA

Downstream 100 bases:

>100_bases
AATAGGAAAATATAATGAATACGAATAAATTTTACTATGAACTTATGAAGCTAAATGTTTTAAGTAGTAGCTATGTTGAT
GATAGCTATAAAGCAGCGTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNWEMVIGLEVHIQLSTKSKLFSTSATKYGQHQNTQAAFLDLGLPGTLPVVNKEAIRKAVIFGLAVDAKISKDSFFARKN
YFYPDLSKGYQISQSTNPIVQEGRLEIETSKGLKTIRIERAHLEEDAGKSVHGYIAGETGLDYNRAGTPLLEIVTYPDFR
SAEEVVAYLKKLHQLVKHLGICDGNMQEGSFRCDVNLSIRPQGQAKFGTRAELKNINSFRFIDKAIEYEYARQVSVLESG
GEVVQETRLYDADANETRSMRAKEDAFDYRYFPDPDLLPLVITDEYIESIKKQMPLKSEEREAVYREHLAEQEVEFLLSN
LEIADYYDKVAVVIGYKPAYNWITVDLISTLNRAEKEFSSDVVPAEILLEIIANVQKDIISQANAKKVIAEYIDAPSAIE
AIIEKLGLKQVSDEGMIRELVQGIIAANPQQAADFKAGKTKLMSFFVGQAMKASKGKANPKQVNQIVQEELNK

Sequences:

>Translated_473_residues
MNWEMVIGLEVHIQLSTKSKLFSTSATKYGQHQNTQAAFLDLGLPGTLPVVNKEAIRKAVIFGLAVDAKISKDSFFARKN
YFYPDLSKGYQISQSTNPIVQEGRLEIETSKGLKTIRIERAHLEEDAGKSVHGYIAGETGLDYNRAGTPLLEIVTYPDFR
SAEEVVAYLKKLHQLVKHLGICDGNMQEGSFRCDVNLSIRPQGQAKFGTRAELKNINSFRFIDKAIEYEYARQVSVLESG
GEVVQETRLYDADANETRSMRAKEDAFDYRYFPDPDLLPLVITDEYIESIKKQMPLKSEEREAVYREHLAEQEVEFLLSN
LEIADYYDKVAVVIGYKPAYNWITVDLISTLNRAEKEFSSDVVPAEILLEIIANVQKDIISQANAKKVIAEYIDAPSAIE
AIIEKLGLKQVSDEGMIRELVQGIIAANPQQAADFKAGKTKLMSFFVGQAMKASKGKANPKQVNQIVQEELNK
>Mature_473_residues
MNWEMVIGLEVHIQLSTKSKLFSTSATKYGQHQNTQAAFLDLGLPGTLPVVNKEAIRKAVIFGLAVDAKISKDSFFARKN
YFYPDLSKGYQISQSTNPIVQEGRLEIETSKGLKTIRIERAHLEEDAGKSVHGYIAGETGLDYNRAGTPLLEIVTYPDFR
SAEEVVAYLKKLHQLVKHLGICDGNMQEGSFRCDVNLSIRPQGQAKFGTRAELKNINSFRFIDKAIEYEYARQVSVLESG
GEVVQETRLYDADANETRSMRAKEDAFDYRYFPDPDLLPLVITDEYIESIKKQMPLKSEEREAVYREHLAEQEVEFLLSN
LEIADYYDKVAVVIGYKPAYNWITVDLISTLNRAEKEFSSDVVPAEILLEIIANVQKDIISQANAKKVIAEYIDAPSAIE
AIIEKLGLKQVSDEGMIRELVQGIIAANPQQAADFKAGKTKLMSFFVGQAMKASKGKANPKQVNQIVQEELNK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=499, Percent_Identity=34.4689378757515, Blast_Score=289, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI115534094, Length=376, Percent_Identity=34.8404255319149, Blast_Score=208, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=29.2307692307692, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45550808, Length=490, Percent_Identity=32.4489795918367, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_FRAT1 (Q14K48)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_666228.1
- ProteinModelPortal:   Q14K48
- STRING:   Q14K48
- GeneID:   4199274
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF0021
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q14K48
- ProtClustDB:   PRK05477
- BioCyc:   FTUL393115:FTF0021-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53093; Mature: 53093

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWEMVIGLEVHIQLSTKSKLFSTSATKYGQHQNTQAAFLDLGLPGTLPVVNKEAIRKAV
CCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHH
IFGLAVDAKISKDSFFARKNYFYPDLSKGYQISQSTNPIVQEGRLEIETSKGLKTIRIER
HHHEEECCEECCCHHHHHCCCCCCCCCCCEEECCCCCCHHHCCCEEEECCCCCEEEEEEH
AHLEEDAGKSVHGYIAGETGLDYNRAGTPLLEIVTYPDFRSAEEVVAYLKKLHQLVKHLG
HHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
ICDGNMQEGSFRCDVNLSIRPQGQAKFGTRAELKNINSFRFIDKAIEYEYARQVSVLESG
CCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
GEVVQETRLYDADANETRSMRAKEDAFDYRYFPDPDLLPLVITDEYIESIKKQMPLKSEE
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCCCHH
REAVYREHLAEQEVEFLLSNLEIADYYDKVAVVIGYKPAYNWITVDLISTLNRAEKEFSS
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHC
DVVPAEILLEIIANVQKDIISQANAKKVIAEYIDAPSAIEAIIEKLGLKQVSDEGMIREL
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHCCHHCCCCHHHHHH
VQGIIAANPQQAADFKAGKTKLMSFFVGQAMKASKGKANPKQVNQIVQEELNK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNWEMVIGLEVHIQLSTKSKLFSTSATKYGQHQNTQAAFLDLGLPGTLPVVNKEAIRKAV
CCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHH
IFGLAVDAKISKDSFFARKNYFYPDLSKGYQISQSTNPIVQEGRLEIETSKGLKTIRIER
HHHEEECCEECCCHHHHHCCCCCCCCCCCEEECCCCCCHHHCCCEEEECCCCCEEEEEEH
AHLEEDAGKSVHGYIAGETGLDYNRAGTPLLEIVTYPDFRSAEEVVAYLKKLHQLVKHLG
HHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
ICDGNMQEGSFRCDVNLSIRPQGQAKFGTRAELKNINSFRFIDKAIEYEYARQVSVLESG
CCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
GEVVQETRLYDADANETRSMRAKEDAFDYRYFPDPDLLPLVITDEYIESIKKQMPLKSEE
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCCCHH
REAVYREHLAEQEVEFLLSNLEIADYYDKVAVVIGYKPAYNWITVDLISTLNRAEKEFSS
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHC
DVVPAEILLEIIANVQKDIISQANAKKVIAEYIDAPSAIEAIIEKLGLKQVSDEGMIREL
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHCCHHCCCCHHHHHH
VQGIIAANPQQAADFKAGKTKLMSFFVGQAMKASKGKANPKQVNQIVQEELNK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA