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The map label for this gene is gatA [H]

Identifier: 134301237

GI number: 134301237

Start: 103067

End: 104512

Strand: Direct

Name: gatA [H]

Synonym: FTW_0094

Alternate gene names: 134301237

Gene position: 103067-104512 (Clockwise)

Preceding gene: 134301236

Following gene: 134301238

Centisome position: 5.43

GC content: 37.48

Gene sequence:

>1446_bases
ATGTCATATATTAAGAAATTAAGAGCTAGATTAGATAGTGGTGAAATAAGCGCTGTTGAACTAACTAAAGAGTATCTAGC
AAAAATTAAAGAGCAAGATAAGCGCATAAACTCTGTAATCACTTTATGTGAAGCAGAAGCACTAAAAGAAGCAGAAGATG
CTGATGCTATTATTTCTGCAGGTAAGCAAGGATTATTAACAGGAATTCCTATTCTACATAAGGATCTTTTTTGCACTAAA
GGTATAAGAACTACGGCTGCCTCTAAGATGTTGGATAATTTCGTAGCACCATATGACTCAACAGTGACAAAAAACTGTAA
GGATCAAGGTATGGTCACTCTAGGCAAACTAAATATGGATGAGTTTGCTATGGGTTCAACTAACGAGTATAGCTATTATG
GAGCAGTTAGTAATCCTTGGGATTTAGAAAGAGTTCCGGGAGGTTCATCAGGTGGCTCAGCTGCAGCAGTTGCTGCAGGT
TTTGCGCCTATAAGTACAGGGTCAGATACTGGTGGATCGGTGAGACAGCCGGCTAGTTTTTGTGGTCTTACAGCAATGAA
GCCTAGTTATGGAAGTACTTCAAGGTTTGGCATGGTAGCGTTTGCATCATCTTTTGATCAAGCTGGTATTTTTGGACACT
ATGCTGAAGATGTCGCGATCATGTTAGATGCAATTGCTGGCGAGTGTGAGTTTGATTCTACTTGTGTTGGTGTTAAACAA
AATCATTTCACCCAAGATCTAGAAAAAGATATCTCTGGTAAAGTAATTGGCGTTGATGAGAGTCTAATCAAAGACCTACC
AGCACAAATACAAGAAGCAGTATCAAAAACTCTTGATAATTTCAAAAAGCTAGGTGCTGAAATAAAGTCGGTCAAGGTCC
CAGATTTAAAAGAAGCTCTTTCAACTTATTATATTATAACTCCAGCAGAAGCAGCAGCTAACTTAGCTAGATATGATGGT
ATTAGATATGGTTACCGTAATCCAGAAGCTAGAGATTTAGATGAATTATATAGAAAATCGCGAACAGATGGTTTTGGTGC
AGAAGTTAAGCGTAGAATTATGATTGGTAACTATGTCCTAGCATCGAGTCAGTATGATTCTTACTACAATAAGGCTCAGC
AGCTACGTAAGGTGATGACTGATCAAATTAATCAAATATTTACCCAAGTTGATGCTATCTTTATGCCAGCATCTCCAAGT
GAGGCATTTAAAAAAGGAGATAAGCTAGATCCTGTTTCGGCATATTTATCAGATATTTACACTATTCCTGCTAACATATC
AGGCCTACCAGCTATTGCTTTCCCAATTGGTTTTGCTAATAATTTACCAGTTGGTGGACAACTTATGGCTAAAGCATTTA
ATGATAATATCTTGACGCAGATGGTAGTGCAGTATCAAAAGCATTATGGTATTGAAGAATTTATTTTACAACAAGCGAGG
ATTTAA

Upstream 100 bases:

>100_bases
TCCCTCAAGATCAAGATAATCGTACAAGTTTTGATAAATTCGCTTGTGAAGTTGTTGATGATTACTTTATGGTGCCACAA
GTTGTTAAATAGGGTGAGCA

Downstream 100 bases:

>100_bases
TAGTGAATTGGGAAATGGTGATAGGGCTAGAAGTCCATATTCAATTAAGTACTAAATCTAAGCTATTTTCGACTTCTGCA
ACAAAGTATGGTCAACATCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSYIKKLRARLDSGEISAVELTKEYLAKIKEQDKRINSVITLCEAEALKEAEDADAIISAGKQGLLTGIPILHKDLFCTK
GIRTTAASKMLDNFVAPYDSTVTKNCKDQGMVTLGKLNMDEFAMGSTNEYSYYGAVSNPWDLERVPGGSSGGSAAAVAAG
FAPISTGSDTGGSVRQPASFCGLTAMKPSYGSTSRFGMVAFASSFDQAGIFGHYAEDVAIMLDAIAGECEFDSTCVGVKQ
NHFTQDLEKDISGKVIGVDESLIKDLPAQIQEAVSKTLDNFKKLGAEIKSVKVPDLKEALSTYYIITPAEAAANLARYDG
IRYGYRNPEARDLDELYRKSRTDGFGAEVKRRIMIGNYVLASSQYDSYYNKAQQLRKVMTDQINQIFTQVDAIFMPASPS
EAFKKGDKLDPVSAYLSDIYTIPANISGLPAIAFPIGFANNLPVGGQLMAKAFNDNILTQMVVQYQKHYGIEEFILQQAR
I

Sequences:

>Translated_481_residues
MSYIKKLRARLDSGEISAVELTKEYLAKIKEQDKRINSVITLCEAEALKEAEDADAIISAGKQGLLTGIPILHKDLFCTK
GIRTTAASKMLDNFVAPYDSTVTKNCKDQGMVTLGKLNMDEFAMGSTNEYSYYGAVSNPWDLERVPGGSSGGSAAAVAAG
FAPISTGSDTGGSVRQPASFCGLTAMKPSYGSTSRFGMVAFASSFDQAGIFGHYAEDVAIMLDAIAGECEFDSTCVGVKQ
NHFTQDLEKDISGKVIGVDESLIKDLPAQIQEAVSKTLDNFKKLGAEIKSVKVPDLKEALSTYYIITPAEAAANLARYDG
IRYGYRNPEARDLDELYRKSRTDGFGAEVKRRIMIGNYVLASSQYDSYYNKAQQLRKVMTDQINQIFTQVDAIFMPASPS
EAFKKGDKLDPVSAYLSDIYTIPANISGLPAIAFPIGFANNLPVGGQLMAKAFNDNILTQMVVQYQKHYGIEEFILQQAR
I
>Mature_480_residues
SYIKKLRARLDSGEISAVELTKEYLAKIKEQDKRINSVITLCEAEALKEAEDADAIISAGKQGLLTGIPILHKDLFCTKG
IRTTAASKMLDNFVAPYDSTVTKNCKDQGMVTLGKLNMDEFAMGSTNEYSYYGAVSNPWDLERVPGGSSGGSAAAVAAGF
APISTGSDTGGSVRQPASFCGLTAMKPSYGSTSRFGMVAFASSFDQAGIFGHYAEDVAIMLDAIAGECEFDSTCVGVKQN
HFTQDLEKDISGKVIGVDESLIKDLPAQIQEAVSKTLDNFKKLGAEIKSVKVPDLKEALSTYYIITPAEAAANLARYDGI
RYGYRNPEARDLDELYRKSRTDGFGAEVKRRIMIGNYVLASSQYDSYYNKAQQLRKVMTDQINQIFTQVDAIFMPASPSE
AFKKGDKLDPVSAYLSDIYTIPANISGLPAIAFPIGFANNLPVGGQLMAKAFNDNILTQMVVQYQKHYGIEEFILQQARI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=34.8514851485148, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI195972892, Length=503, Percent_Identity=28.2306163021869, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI166795287, Length=192, Percent_Identity=31.7708333333333, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=404, Percent_Identity=35.1485148514851, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17556264, Length=305, Percent_Identity=28.1967213114754, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17537465, Length=484, Percent_Identity=22.7272727272727, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=314, Percent_Identity=26.7515923566879, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=199, Percent_Identity=32.1608040201005, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=284, Percent_Identity=28.8732394366197, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=284, Percent_Identity=28.8732394366197, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323950, Length=424, Percent_Identity=34.1981132075472, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6319685, Length=473, Percent_Identity=25.369978858351, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648113, Length=478, Percent_Identity=37.2384937238494, Blast_Score=278, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24652985, Length=499, Percent_Identity=24.8496993987976, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI19922090, Length=499, Percent_Identity=24.8496993987976, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24652981, Length=499, Percent_Identity=24.8496993987976, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24652983, Length=499, Percent_Identity=24.8496993987976, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24644968, Length=492, Percent_Identity=24.1869918699187, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=24.8, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI45550774, Length=494, Percent_Identity=23.6842105263158, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648435, Length=494, Percent_Identity=23.6842105263158, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24648437, Length=494, Percent_Identity=23.6842105263158, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24648441, Length=425, Percent_Identity=23.7647058823529, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648439, Length=425, Percent_Identity=23.7647058823529, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356731, Length=218, Percent_Identity=26.1467889908257, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52352; Mature: 52221

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYIKKLRARLDSGEISAVELTKEYLAKIKEQDKRINSVITLCEAEALKEAEDADAIISA
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKQGLLTGIPILHKDLFCTKGIRTTAASKMLDNFVAPYDSTVTKNCKDQGMVTLGKLNMD
CCCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEECCCHH
EFAMGSTNEYSYYGAVSNPWDLERVPGGSSGGSAAAVAAGFAPISTGSDTGGSVRQPASF
HHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHH
CGLTAMKPSYGSTSRFGMVAFASSFDQAGIFGHYAEDVAIMLDAIAGECEFDSTCVGVKQ
HCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCHH
NHFTQDLEKDISGKVIGVDESLIKDLPAQIQEAVSKTLDNFKKLGAEIKSVKVPDLKEAL
HHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
STYYIITPAEAAANLARYDGIRYGYRNPEARDLDELYRKSRTDGFGAEVKRRIMIGNYVL
HCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHEECCEEE
ASSQYDSYYNKAQQLRKVMTDQINQIFTQVDAIFMPASPSEAFKKGDKLDPVSAYLSDIY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCCCHHHHHHHHHH
TIPANISGLPAIAFPIGFANNLPVGGQLMAKAFNDNILTQMVVQYQKHYGIEEFILQQAR
CCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHC
I
C
>Mature Secondary Structure 
SYIKKLRARLDSGEISAVELTKEYLAKIKEQDKRINSVITLCEAEALKEAEDADAIISA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKQGLLTGIPILHKDLFCTKGIRTTAASKMLDNFVAPYDSTVTKNCKDQGMVTLGKLNMD
CCCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEECCCHH
EFAMGSTNEYSYYGAVSNPWDLERVPGGSSGGSAAAVAAGFAPISTGSDTGGSVRQPASF
HHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHH
CGLTAMKPSYGSTSRFGMVAFASSFDQAGIFGHYAEDVAIMLDAIAGECEFDSTCVGVKQ
HCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCHH
NHFTQDLEKDISGKVIGVDESLIKDLPAQIQEAVSKTLDNFKKLGAEIKSVKVPDLKEAL
HHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
STYYIITPAEAAANLARYDGIRYGYRNPEARDLDELYRKSRTDGFGAEVKRRIMIGNYVL
HCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHEECCEEE
ASSQYDSYYNKAQQLRKVMTDQINQIFTQVDAIFMPASPSEAFKKGDKLDPVSAYLSDIY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCCCHHHHHHHHHH
TIPANISGLPAIAFPIGFANNLPVGGQLMAKAFNDNILTQMVVQYQKHYGIEEFILQQAR
CCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA