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The map label for this gene is folC [H]

Identifier: 134300391

GI number: 134300391

Start: 2775647

End: 2776942

Strand: Reverse

Name: folC [H]

Synonym: Dred_2552

Alternate gene names: 134300391

Gene position: 2776942-2775647 (Counterclockwise)

Preceding gene: 134300392

Following gene: 134300390

Centisome position: 76.96

GC content: 42.44

Gene sequence:

>1296_bases
GTGACCTACAACGAAGCAATTAACTACCTGAAAAGTTTAACGAAGTTTGGTATGAACCTGGGTCTGGCTCGCATTGAGGA
ACTATTGAGACGTTTACAGAATCCTCACCATAGACTTAATATTGTCCATATCGGTGGTACCAATGGCAAAGGCTCTACCA
CCGCCATGATTGCCAACATTCTAAAAACAGCAGGGTATAAAACAGGGATGTTTACTTCTCCTCACCTACAGGATTATACC
GAACGATTTTTGATTAATGGTCAACCCATTCCTCAAGAAACACTTGCGTCTTTGATCCAATATATCAGACCACACCTTGA
CAAAATGGTACAGGAGGGGTTTGAGCATCCCACCGAGTTTGAAGTGAGTACTGCGTTGGCATTGCTTTACTTTGCCAGAG
AAGAAACTGATTTTGTAGCTCTGGAAGTGGGGTTAGGTGGAGCCATTGATTCTACCAATGTGGTTAAACCCAAAGTGTCG
GTTATCACTAATATAGCAATGGATCACATGGATTATTTAGGAAGCACCTTGGTGGACATAGCCAACGTAAAGGCAGGTAT
CATTAAACAAGGTATACCAGTGGTTACTGCTGCCGAAGGAGAAGCCCTTGGGGTTATCCGTCAAAGAGCATTAGTTAACA
ACAGTAAGTTAATACAAATTGGTAAAGAAGTTACCTGGAAGCCACAAAAAAATAAATTTAAGCAAGGGTTTCAGGAATTT
ATCATAAATGGACTACAAAATCAATATTATGTGAGACTTCCTCTGCTGGGCGAACATCAGCAAGTCAATGCTGCCTCAGC
GGTGGCTGCGGTGGAATTGCTTTTTACTGGGAATGGTTATCATCTACCTTCTGCTGTGGTGATAGAAGGTTTGAGGACGG
TTGAATGGCCAGGTCGGTTGGAGATCATTCGGAGAAATCCAACCACTATTTTAGACGGCGCCCATAACCATGCCGGTGCC
ATAGCTTTAAAAAAGGCATTAGAAAGTTGCTTTCCTAGTCAGAAAATTATTTTTGTTTTAGGAATGCTGGCAGACAAGGA
ACGGTCTAAAGTTGTGGCAGAATTGGCCCCTTTGAGTAAAGCTGTGGTGGTTACACGCTCCAACAATCCTCGGGCCGGAG
ACTGGAGGAAGTTGGCTGATTATGTTCGTACCTATATAGAGGATGTTATTGTTGTAGAAAATGTGGCTGAGGCAATTAAT
CAGGCATTGGGCCATGTAAAAGAGAGCGAAATATTGTGTATTACCGGTTCATTATATATGATTGCAGAAGCAAGGGCTTA
TCTGATGCAATCTTAA

Upstream 100 bases:

>100_bases
GACCCTTAAAGCAATACATGCGCAGAAAAGCCCAAGGCTTAAGGCTCATGGCCAAAATCTAATAGCTCAGCAATATTTCC
ATTAAAGAGAGGCGTACTCC

Downstream 100 bases:

>100_bases
ATATATTCTTTCAACAGGGATTCCGGGAAATTTACAATGGAATAATAAATCCAAGGCGACTGGTATTTAAAGAAAGATAA
AATCTTTATGTAAAAACAAT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTYNEAINYLKSLTKFGMNLGLARIEELLRRLQNPHHRLNIVHIGGTNGKGSTTAMIANILKTAGYKTGMFTSPHLQDYT
ERFLINGQPIPQETLASLIQYIRPHLDKMVQEGFEHPTEFEVSTALALLYFAREETDFVALEVGLGGAIDSTNVVKPKVS
VITNIAMDHMDYLGSTLVDIANVKAGIIKQGIPVVTAAEGEALGVIRQRALVNNSKLIQIGKEVTWKPQKNKFKQGFQEF
IINGLQNQYYVRLPLLGEHQQVNAASAVAAVELLFTGNGYHLPSAVVIEGLRTVEWPGRLEIIRRNPTTILDGAHNHAGA
IALKKALESCFPSQKIIFVLGMLADKERSKVVAELAPLSKAVVVTRSNNPRAGDWRKLADYVRTYIEDVIVVENVAEAIN
QALGHVKESEILCITGSLYMIAEARAYLMQS

Sequences:

>Translated_431_residues
MTYNEAINYLKSLTKFGMNLGLARIEELLRRLQNPHHRLNIVHIGGTNGKGSTTAMIANILKTAGYKTGMFTSPHLQDYT
ERFLINGQPIPQETLASLIQYIRPHLDKMVQEGFEHPTEFEVSTALALLYFAREETDFVALEVGLGGAIDSTNVVKPKVS
VITNIAMDHMDYLGSTLVDIANVKAGIIKQGIPVVTAAEGEALGVIRQRALVNNSKLIQIGKEVTWKPQKNKFKQGFQEF
IINGLQNQYYVRLPLLGEHQQVNAASAVAAVELLFTGNGYHLPSAVVIEGLRTVEWPGRLEIIRRNPTTILDGAHNHAGA
IALKKALESCFPSQKIIFVLGMLADKERSKVVAELAPLSKAVVVTRSNNPRAGDWRKLADYVRTYIEDVIVVENVAEAIN
QALGHVKESEILCITGSLYMIAEARAYLMQS
>Mature_430_residues
TYNEAINYLKSLTKFGMNLGLARIEELLRRLQNPHHRLNIVHIGGTNGKGSTTAMIANILKTAGYKTGMFTSPHLQDYTE
RFLINGQPIPQETLASLIQYIRPHLDKMVQEGFEHPTEFEVSTALALLYFAREETDFVALEVGLGGAIDSTNVVKPKVSV
ITNIAMDHMDYLGSTLVDIANVKAGIIKQGIPVVTAAEGEALGVIRQRALVNNSKLIQIGKEVTWKPQKNKFKQGFQEFI
INGLQNQYYVRLPLLGEHQQVNAASAVAAVELLFTGNGYHLPSAVVIEGLRTVEWPGRLEIIRRNPTTILDGAHNHAGAI
ALKKALESCFPSQKIIFVLGMLADKERSKVVAELAPLSKAVVVTRSNNPRAGDWRKLADYVRTYIEDVIVVENVAEAINQ
ALGHVKESEILCITGSLYMIAEARAYLMQS

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=313, Percent_Identity=36.1022364217252, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI66932990, Length=315, Percent_Identity=35.8730158730159, Blast_Score=165, Evalue=7e-41,
Organism=Escherichia coli, GI1788654, Length=416, Percent_Identity=31.0096153846154, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17553150, Length=498, Percent_Identity=27.1084337349398, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17553148, Length=498, Percent_Identity=27.1084337349398, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71984923, Length=498, Percent_Identity=27.1084337349398, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6323760, Length=432, Percent_Identity=30.787037037037, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6324815, Length=363, Percent_Identity=31.6804407713499, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6322718, Length=367, Percent_Identity=24.5231607629428, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24641571, Length=288, Percent_Identity=32.6388888888889, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24581568, Length=300, Percent_Identity=26.6666666666667, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47565; Mature: 47434

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYNEAINYLKSLTKFGMNLGLARIEELLRRLQNPHHRLNIVHIGGTNGKGSTTAMIANI
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHH
LKTAGYKTGMFTSPHLQDYTERFLINGQPIPQETLASLIQYIRPHLDKMVQEGFEHPTEF
HHHCCCCCCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EVSTALALLYFAREETDFVALEVGLGGAIDSTNVVKPKVSVITNIAMDHMDYLGSTLVDI
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ANVKAGIIKQGIPVVTAAEGEALGVIRQRALVNNSKLIQIGKEVTWKPQKNKFKQGFQEF
HHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHH
IINGLQNQYYVRLPLLGEHQQVNAASAVAAVELLFTGNGYHLPSAVVIEGLRTVEWPGRL
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHEEEEEECCCCCCCHHHHHHCCHHCCCCCCE
EIIRRNPTTILDGAHNHAGAIALKKALESCFPSQKIIFVLGMLADKERSKVVAELAPLSK
EEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCE
AVVVTRSNNPRAGDWRKLADYVRTYIEDVIVVENVAEAINQALGHVKESEILCITGSLYM
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHH
IAEARAYLMQS
HHHHHHHHHCC
>Mature Secondary Structure 
TYNEAINYLKSLTKFGMNLGLARIEELLRRLQNPHHRLNIVHIGGTNGKGSTTAMIANI
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHH
LKTAGYKTGMFTSPHLQDYTERFLINGQPIPQETLASLIQYIRPHLDKMVQEGFEHPTEF
HHHCCCCCCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EVSTALALLYFAREETDFVALEVGLGGAIDSTNVVKPKVSVITNIAMDHMDYLGSTLVDI
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ANVKAGIIKQGIPVVTAAEGEALGVIRQRALVNNSKLIQIGKEVTWKPQKNKFKQGFQEF
HHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHH
IINGLQNQYYVRLPLLGEHQQVNAASAVAAVELLFTGNGYHLPSAVVIEGLRTVEWPGRL
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHEEEEEECCCCCCCHHHHHHCCHHCCCCCCE
EIIRRNPTTILDGAHNHAGAIALKKALESCFPSQKIIFVLGMLADKERSKVVAELAPLSK
EEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCE
AVVVTRSNNPRAGDWRKLADYVRTYIEDVIVVENVAEAINQALGHVKESEILCITGSLYM
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHH
IAEARAYLMQS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]