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The map label for this gene is rbsB [H]

Identifier: 134300361

GI number: 134300361

Start: 2741612

End: 2742559

Strand: Reverse

Name: rbsB [H]

Synonym: Dred_2522

Alternate gene names: 134300361

Gene position: 2742559-2741612 (Counterclockwise)

Preceding gene: 134300362

Following gene: 134300359

Centisome position: 76.01

GC content: 45.04

Gene sequence:

>948_bases
ATGAAGAAATTTTGGAAACTTGGAATTGTGTTAGGCAGTCTTATACTTATGATAGGTTTGGTCATTGGTTGTGCCCAGAA
AAAGGAGGAACCAAAGGAAAGTCCCAAGGCGGCCACTGAAGAAAAGGATAAAATGACTATTGGCCTTGCCGTATCCACAT
TAAACAACCCATTTTTTGTAGATATGAGGGATGGCGCAAAAGCTGCAGCGGATAAAATGAATGCTGAACTGGTGGTTGTG
GATGCCCAGGACGATGCATCCAAACAGGTTTCACAAGTAGAAAATTTAATTCAGCAAAAGGTTGATATTATTCTGTTAAA
CCCCACCGACAGTGATGGGTTAGTAACCGCAGTAAAAGACGCCAACGAAGCTAAAATTCCTGTTATTACGCTGGACCGTA
ATGTAAACGGTGGTACCGTAACCTCCCATATTGCCTCGGACAATGTGGCCGGTGGTAGAATGGCTGCCGAGTACATTATC
AAACAATTGGACCGTAAAGGCAAAGTGGTTGAGCTAGAAGGAATTGCCGGTACTTCTGCCGCCAGGGACCGTGGACAGGG
CTTCCATGAAGTGATGGATAAAGAAAAAAACATCAAAGTTGTGGCCAAGCAACCAGCGGACTTTGATCGTTCTAAAGGCC
TTCGGGTAATGGAAAATATTCTGCAAAGCCAGCCGGAAATTAACGCAGTATTTGCTCACAACGATGAAATGGCACTGGGC
GCCCTGCAGGCAGTCGCCGCTGCTAAAAAGGATATTATGGTGGTTGGCTTTGATGGTAATGACGATGCTGTCAATGCGGT
TAACGATGGTAAAATGGCTGCCACAGTGGCTCAACAGCCGAAATTAATCGGTCAGCTGGGTGTAGAAACGGCCCAAAGGG
TCGTTAAAGGCGAAAAGGTGGATACCAATATCCCGGCTAAGTTAAAACTAATAACCAAGAAAGAATAA

Upstream 100 bases:

>100_bases
GTTGCGGTTCTGTTAGACCGACGGAATGCTGTGGGGAGGTGATGGGGTAACGATTCTTTTTACATATTTAAGTATATCTT
AACAATAGGAGGAAACTAGC

Downstream 100 bases:

>100_bases
AATGAGAAGCGTCCAAGATTTCTTGGACGCTTCTATTTGACTTCTAATACATAACAATAACCGTTCCAATAAAAATCATA
TAAAACACACTGTTGAAGGC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKKFWKLGIVLGSLILMIGLVIGCAQKKEEPKESPKAATEEKDKMTIGLAVSTLNNPFFVDMRDGAKAAADKMNAELVVV
DAQDDASKQVSQVENLIQQKVDIILLNPTDSDGLVTAVKDANEAKIPVITLDRNVNGGTVTSHIASDNVAGGRMAAEYII
KQLDRKGKVVELEGIAGTSAARDRGQGFHEVMDKEKNIKVVAKQPADFDRSKGLRVMENILQSQPEINAVFAHNDEMALG
ALQAVAAAKKDIMVVGFDGNDDAVNAVNDGKMAATVAQQPKLIGQLGVETAQRVVKGEKVDTNIPAKLKLITKKE

Sequences:

>Translated_315_residues
MKKFWKLGIVLGSLILMIGLVIGCAQKKEEPKESPKAATEEKDKMTIGLAVSTLNNPFFVDMRDGAKAAADKMNAELVVV
DAQDDASKQVSQVENLIQQKVDIILLNPTDSDGLVTAVKDANEAKIPVITLDRNVNGGTVTSHIASDNVAGGRMAAEYII
KQLDRKGKVVELEGIAGTSAARDRGQGFHEVMDKEKNIKVVAKQPADFDRSKGLRVMENILQSQPEINAVFAHNDEMALG
ALQAVAAAKKDIMVVGFDGNDDAVNAVNDGKMAATVAQQPKLIGQLGVETAQRVVKGEKVDTNIPAKLKLITKKE
>Mature_315_residues
MKKFWKLGIVLGSLILMIGLVIGCAQKKEEPKESPKAATEEKDKMTIGLAVSTLNNPFFVDMRDGAKAAADKMNAELVVV
DAQDDASKQVSQVENLIQQKVDIILLNPTDSDGLVTAVKDANEAKIPVITLDRNVNGGTVTSHIASDNVAGGRMAAEYII
KQLDRKGKVVELEGIAGTSAARDRGQGFHEVMDKEKNIKVVAKQPADFDRSKGLRVMENILQSQPEINAVFAHNDEMALG
ALQAVAAAKKDIMVVGFDGNDDAVNAVNDGKMAATVAQQPKLIGQLGVETAQRVVKGEKVDTNIPAKLKLITKKE

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=272, Percent_Identity=57.3529411764706, Blast_Score=304, Evalue=4e-84,
Organism=Escherichia coli, GI1790526, Length=267, Percent_Identity=37.0786516853933, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1790674, Length=256, Percent_Identity=33.984375, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790194, Length=218, Percent_Identity=25.6880733944954, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789990, Length=273, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788898, Length=215, Percent_Identity=28.8372093023256, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786540, Length=278, Percent_Identity=25.5395683453237, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787948, Length=280, Percent_Identity=25, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790715, Length=222, Percent_Identity=24.7747747747748, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33778; Mature: 33778

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFWKLGIVLGSLILMIGLVIGCAQKKEEPKESPKAATEEKDKMTIGLAVSTLNNPFFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEE
DMRDGAKAAADKMNAELVVVDAQDDASKQVSQVENLIQQKVDIILLNPTDSDGLVTAVKD
EECCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEC
ANEAKIPVITLDRNVNGGTVTSHIASDNVAGGRMAAEYIIKQLDRKGKVVELEGIAGTSA
CCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCH
ARDRGQGFHEVMDKEKNIKVVAKQPADFDRSKGLRVMENILQSQPEINAVFAHNDEMALG
HHHHCCCHHHHHCCCCCEEEEEECCCCCCHHCCHHHHHHHHHCCCCCEEEEECCCCHHHH
ALQAVAAAKKDIMVVGFDGNDDAVNAVNDGKMAATVAQQPKLIGQLGVETAQRVVKGEKV
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
DTNIPAKLKLITKKE
CCCCCCEEEEEECCC
>Mature Secondary Structure
MKKFWKLGIVLGSLILMIGLVIGCAQKKEEPKESPKAATEEKDKMTIGLAVSTLNNPFFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEE
DMRDGAKAAADKMNAELVVVDAQDDASKQVSQVENLIQQKVDIILLNPTDSDGLVTAVKD
EECCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEC
ANEAKIPVITLDRNVNGGTVTSHIASDNVAGGRMAAEYIIKQLDRKGKVVELEGIAGTSA
CCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCH
ARDRGQGFHEVMDKEKNIKVVAKQPADFDRSKGLRVMENILQSQPEINAVFAHNDEMALG
HHHHCCCHHHHHCCCCCEEEEEECCCCCCHHCCHHHHHHHHHCCCCCEEEEECCCCHHHH
ALQAVAAAKKDIMVVGFDGNDDAVNAVNDGKMAATVAQQPKLIGQLGVETAQRVVKGEKV
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
DTNIPAKLKLITKKE
CCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]