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The map label for this gene is hemN [H]

Identifier: 134300339

GI number: 134300339

Start: 2720798

End: 2721934

Strand: Reverse

Name: hemN [H]

Synonym: Dred_2500

Alternate gene names: 134300339

Gene position: 2721934-2720798 (Counterclockwise)

Preceding gene: 134300340

Following gene: 134300338

Centisome position: 75.44

GC content: 42.92

Gene sequence:

>1137_bases
ATGTCAATAGGTGTGTACATCCATGTACCCTTTTGTGTTCGCAAATGTCTATACTGTGATTTTACTTCCTATCCAGTCAA
CCGGGAACAGGTATCTCTGTATCTCCGGGGACTGGAGAAGGAAATTGCTTATTACAGTGAATCCCTTTTGTTAAAGGAAA
AAACAATTAATACAATTTTTGTGGGCGGCGGTACGCCCACTTCTCTTATGTCCGGGCAACTGGCCAGCATTTTGGAACGG
CTGGACCGTTCCTTTTATATTGCCAATGATGCAGAAATTACCACCGAGGCTAACCCAGGAACTGTGAATTTTTCTTACCT
TAAGGACCTCAGGGCAGCCGGTTTTAACCGGCTGAGTCTGGGGGTACAGTCCACCCACCAAGAACTTCTAAATGAAATTG
GTCGCATCCATAATTTTGAACAAGCCAAGGCAGCTGTTTGGCAGGCCCGGCAAGCAAGCTTCCACAATATCAATTTGGAC
CTTATTTTTGGTTTACCCAAACAAACAGTTAAACAATGGCAGCAGTCTTTAACTGAAATCATAAACCTAGAGCCGCAACA
TATTTCTTGCTATGGGCTGCAGTTGGAAGAAGGAACCCCCTTAACCTGTTCCATAGAACAGGGCAAGTTGGAGCCTTGCC
AAGAAGAGGACGAACTGGAAATGTATCAGCTGGCAATACGTATACTGGGATCAATGGGTTATGAGCACTATGAAATCTCA
AATTTTGCCCGCCCAGGTTATCAATGCCGGCATAATCTATTATATTGGCACAATGGGGAATACTTGGGCCTCGGCCCTGC
GGCCCATTCTCATTTAAATCAGGTACGTTGGGGTAATGTGGGGAATATTGAGCAATATGCCTCTTTACTGAAAAGGGGCG
ATATACCTAGGGAAGAGAAAACAATACTTTCCGTCAGGGAGCAAATGGAAGAAACAGTTTTCCTGGGCTTACGAATGTTA
AAAGGATTAAAGCTAGAGAACTTTCGAATGCGTTTTGGGTTACCAATTACCAAAGTTTTCAAGAAAGAAATTAGCCGGAT
GGAGCAACTAGGTTTAGTAAAATTAGAGGATGGTTATCTTAAATTAACAGAGAAAGGTTTACCACTGGCAAATCAAGTTT
TTGCTGAGTTTGTATAA

Upstream 100 bases:

>100_bases
GAAGCTGTCCTCAAGTCTGGCCGGTGGTGGAAGCTTGGCTTAAGGAGCCGGGGCAGGAAGAAGGTCTGATAAAGGAAATA
CAAAACAAGCTGGTGGAACC

Downstream 100 bases:

>100_bases
AAATGAATATTATTTACACTAGGTTTGGAAAAATTGCTTTATAAACCCTAAAAACCTAAGATACAGGGCAATCTGGTATC
CTTGACAAATAATTTTCCTT

Product: putative oxygen-independent coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSIGVYIHVPFCVRKCLYCDFTSYPVNREQVSLYLRGLEKEIAYYSESLLLKEKTINTIFVGGGTPTSLMSGQLASILER
LDRSFYIANDAEITTEANPGTVNFSYLKDLRAAGFNRLSLGVQSTHQELLNEIGRIHNFEQAKAAVWQARQASFHNINLD
LIFGLPKQTVKQWQQSLTEIINLEPQHISCYGLQLEEGTPLTCSIEQGKLEPCQEEDELEMYQLAIRILGSMGYEHYEIS
NFARPGYQCRHNLLYWHNGEYLGLGPAAHSHLNQVRWGNVGNIEQYASLLKRGDIPREEKTILSVREQMEETVFLGLRML
KGLKLENFRMRFGLPITKVFKKEISRMEQLGLVKLEDGYLKLTEKGLPLANQVFAEFV

Sequences:

>Translated_378_residues
MSIGVYIHVPFCVRKCLYCDFTSYPVNREQVSLYLRGLEKEIAYYSESLLLKEKTINTIFVGGGTPTSLMSGQLASILER
LDRSFYIANDAEITTEANPGTVNFSYLKDLRAAGFNRLSLGVQSTHQELLNEIGRIHNFEQAKAAVWQARQASFHNINLD
LIFGLPKQTVKQWQQSLTEIINLEPQHISCYGLQLEEGTPLTCSIEQGKLEPCQEEDELEMYQLAIRILGSMGYEHYEIS
NFARPGYQCRHNLLYWHNGEYLGLGPAAHSHLNQVRWGNVGNIEQYASLLKRGDIPREEKTILSVREQMEETVFLGLRML
KGLKLENFRMRFGLPITKVFKKEISRMEQLGLVKLEDGYLKLTEKGLPLANQVFAEFV
>Mature_377_residues
SIGVYIHVPFCVRKCLYCDFTSYPVNREQVSLYLRGLEKEIAYYSESLLLKEKTINTIFVGGGTPTSLMSGQLASILERL
DRSFYIANDAEITTEANPGTVNFSYLKDLRAAGFNRLSLGVQSTHQELLNEIGRIHNFEQAKAAVWQARQASFHNINLDL
IFGLPKQTVKQWQQSLTEIINLEPQHISCYGLQLEEGTPLTCSIEQGKLEPCQEEDELEMYQLAIRILGSMGYEHYEISN
FARPGYQCRHNLLYWHNGEYLGLGPAAHSHLNQVRWGNVGNIEQYASLLKRGDIPREEKTILSVREQMEETVFLGLRMLK
GLKLENFRMRFGLPITKVFKKEISRMEQLGLVKLEDGYLKLTEKGLPLANQVFAEFV

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=268, Percent_Identity=36.1940298507463, Blast_Score=179, Evalue=3e-45,
Organism=Escherichia coli, GI1789325, Length=278, Percent_Identity=38.1294964028777, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87082341, Length=390, Percent_Identity=27.4358974358974, Blast_Score=134, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 43210; Mature: 43079

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIGVYIHVPFCVRKCLYCDFTSYPVNREQVSLYLRGLEKEIAYYSESLLLKEKTINTIF
CCEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEE
VGGGTPTSLMSGQLASILERLDRSFYIANDAEITTEANPGTVNFSYLKDLRAAGFNRLSL
ECCCCCHHHHCCHHHHHHHHHCCCEEEECCCEEEECCCCCEEEHHHHHHHHHCCCCCEEC
GVQSTHQELLNEIGRIHNFEQAKAAVWQARQASFHNINLDLIFGLPKQTVKQWQQSLTEI
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEECCEEEEECCCHHHHHHHHHHHHHH
INLEPQHISCYGLQLEEGTPLTCSIEQGKLEPCQEEDELEMYQLAIRILGSMGYEHYEIS
HCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECC
NFARPGYQCRHNLLYWHNGEYLGLGPAAHSHLNQVRWGNVGNIEQYASLLKRGDIPREEK
CCCCCCHHHHCCEEEEECCCEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHH
TILSVREQMEETVFLGLRMLKGLKLENFRMRFGLPITKVFKKEISRMEQLGLVKLEDGYL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCEE
KLTEKGLPLANQVFAEFV
EEECCCCCHHHHHHHHCC
>Mature Secondary Structure 
SIGVYIHVPFCVRKCLYCDFTSYPVNREQVSLYLRGLEKEIAYYSESLLLKEKTINTIF
CEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEE
VGGGTPTSLMSGQLASILERLDRSFYIANDAEITTEANPGTVNFSYLKDLRAAGFNRLSL
ECCCCCHHHHCCHHHHHHHHHCCCEEEECCCEEEECCCCCEEEHHHHHHHHHCCCCCEEC
GVQSTHQELLNEIGRIHNFEQAKAAVWQARQASFHNINLDLIFGLPKQTVKQWQQSLTEI
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEECCEEEEECCCHHHHHHHHHHHHHH
INLEPQHISCYGLQLEEGTPLTCSIEQGKLEPCQEEDELEMYQLAIRILGSMGYEHYEIS
HCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECC
NFARPGYQCRHNLLYWHNGEYLGLGPAAHSHLNQVRWGNVGNIEQYASLLKRGDIPREEK
CCCCCCHHHHCCEEEEECCCEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHH
TILSVREQMEETVFLGLRMLKGLKLENFRMRFGLPITKVFKKEISRMEQLGLVKLEDGYL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCEE
KLTEKGLPLANQVFAEFV
EEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421 [H]