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The map label for this gene is rsmE [H]

Identifier: 134300332

GI number: 134300332

Start: 2712456

End: 2713199

Strand: Reverse

Name: rsmE [H]

Synonym: Dred_2493

Alternate gene names: 134300332

Gene position: 2713199-2712456 (Counterclockwise)

Preceding gene: 134300333

Following gene: 134300331

Centisome position: 75.2

GC content: 50.81

Gene sequence:

>744_bases
TTGCCCCGATTTTTTGTCCAACCTGAACAAATTAACAGCGATACAGCCGTCATTAATGGGCCGGATGTAAAACATATCAG
CCGGGTTTTGAGGATGGAGACCGGGAATAACCTAACCCTTCTTGATGGCCGGGGGAATGTTTATTTGGCGCAGATTTTAG
AAATAAATAAACAAGAGGTTCACTGTAGGATTCTGGAGCAGCAAGAGGCCACTTCGGAGCCAACGGTAAAAGTTACCCTG
GTGCAGGGTCTGCCCAAGGGTGATAAGATGGAAACAATTATACAGAAGTGTACTGAACTGGGGGTTAGCAGCATCATTCC
CCTGGCCGCCGCCCGTTCGATTGTAAAGTTGGATGCTAAAAAGGCTGCAGAGCGGGTACAGCGTTGGCAGCGGGTAGCAG
TGGAGGCTGCCAAACAATGCCGGCGGTCCGGTATACCGGAAGTATATATGCTAAGTAGCTGGGATGAAATACTGCAGCAA
ATTCCGCCGGATGCCCTGGTGCTGATGCCCTGGGAGGGTGAAAGGACAAAATCCCTTAGAGAAGTCTTGCAAACAAGGCC
GATGGCACCCGGCCAGGTGTATATTTTTATTGGTCCCGAAGGCGGCTTTGAAGAAGAGGAAGTTGAGCGGGTCAAGGAAA
AGGGATTTTACCCGGTTACACTGGGCAGCCGGATTTTGCGCACTGAAACAGCAGGACCAGCGGCTCTGACAATGGTCCTG
TACCAGTTTGGCGAACTGGGCTAA

Upstream 100 bases:

>100_bases
AGAAGTCCGGCAAAAGTTGGAACAAACCGGTTTTAAAATTCTAGGACGCAAGGAAGACGGGGAATGGGTTTCCTACCTAT
GTATTTTGGAGGGATAAGAT

Downstream 100 bases:

>100_bases
GGAGGTTTCCAATATGGCTAAATCTGCTGCTATTTATACCCTTGGATGTAAAGTTAACCAGTATGAATCATCTGCCATCG
CCGATTTATTTCGGCAAGCG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MPRFFVQPEQINSDTAVINGPDVKHISRVLRMETGNNLTLLDGRGNVYLAQILEINKQEVHCRILEQQEATSEPTVKVTL
VQGLPKGDKMETIIQKCTELGVSSIIPLAAARSIVKLDAKKAAERVQRWQRVAVEAAKQCRRSGIPEVYMLSSWDEILQQ
IPPDALVLMPWEGERTKSLREVLQTRPMAPGQVYIFIGPEGGFEEEEVERVKEKGFYPVTLGSRILRTETAGPAALTMVL
YQFGELG

Sequences:

>Translated_247_residues
MPRFFVQPEQINSDTAVINGPDVKHISRVLRMETGNNLTLLDGRGNVYLAQILEINKQEVHCRILEQQEATSEPTVKVTL
VQGLPKGDKMETIIQKCTELGVSSIIPLAAARSIVKLDAKKAAERVQRWQRVAVEAAKQCRRSGIPEVYMLSSWDEILQQ
IPPDALVLMPWEGERTKSLREVLQTRPMAPGQVYIFIGPEGGFEEEEVERVKEKGFYPVTLGSRILRTETAGPAALTMVL
YQFGELG
>Mature_246_residues
PRFFVQPEQINSDTAVINGPDVKHISRVLRMETGNNLTLLDGRGNVYLAQILEINKQEVHCRILEQQEATSEPTVKVTLV
QGLPKGDKMETIIQKCTELGVSSIIPLAAARSIVKLDAKKAAERVQRWQRVAVEAAKQCRRSGIPEVYMLSSWDEILQQI
PPDALVLMPWEGERTKSLREVLQTRPMAPGQVYIFIGPEGGFEEEEVERVKEKGFYPVTLGSRILRTETAGPAALTMVLY
QFGELG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=256, Percent_Identity=35.15625, Blast_Score=148, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27640; Mature: 27509

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFFVQPEQINSDTAVINGPDVKHISRVLRMETGNNLTLLDGRGNVYLAQILEINKQEV
CCCCCCCHHHCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEHHHHCCCHHH
HCRILEQQEATSEPTVKVTLVQGLPKGDKMETIIQKCTELGVSSIIPLAAARSIVKLDAK
HHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
KAAERVQRWQRVAVEAAKQCRRSGIPEVYMLSSWDEILQQIPPDALVLMPWEGERTKSLR
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCEEEEECCCCCHHHHHH
EVLQTRPMAPGQVYIFIGPEGGFEEEEVERVKEKGFYPVTLGSRILRTETAGPAALTMVL
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEECCCHHHHCCCCCHHHHHHHH
YQFGELG
HHHHCCC
>Mature Secondary Structure 
PRFFVQPEQINSDTAVINGPDVKHISRVLRMETGNNLTLLDGRGNVYLAQILEINKQEV
CCCCCCHHHCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEHHHHCCCHHH
HCRILEQQEATSEPTVKVTLVQGLPKGDKMETIIQKCTELGVSSIIPLAAARSIVKLDAK
HHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
KAAERVQRWQRVAVEAAKQCRRSGIPEVYMLSSWDEILQQIPPDALVLMPWEGERTKSLR
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCEEEEECCCCCHHHHHH
EVLQTRPMAPGQVYIFIGPEGGFEEEEVERVKEKGFYPVTLGSRILRTETAGPAALTMVL
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEECCCHHHHCCCCCHHHHHHHH
YQFGELG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]