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The map label for this gene is glyS

Identifier: 134300311

GI number: 134300311

Start: 2693771

End: 2695858

Strand: Reverse

Name: glyS

Synonym: Dred_2472

Alternate gene names: 134300311

Gene position: 2695858-2693771 (Counterclockwise)

Preceding gene: 134300312

Following gene: 134300310

Centisome position: 74.72

GC content: 47.56

Gene sequence:

>2088_bases
ATGGCTAAGGATTTTTTATTGGAAGTTGGTATCGAAGAAATGCCTGCCCGTTTTTTGGGTCCGGCTTTAACACAATTAAA
GGAACAAACGGTCAAAACCCTGCAGGAACTTAGAATTGAATATGCGGATATACAAACCTATGGTACCCCTCGTCGCCTGG
TACTTTACATAAAAGATCTAGCAGAAAATCAGGCTGCCCTGGAAAAGGAAGTAAAGGGTCCTGCTAAAAAAGCGGCCTTT
GATGCAGCTGGTAATCCTACCAAGGCTATTTTAGGCTTTACCCGTTCCCAGGGGGTATCCATGGAAGATCTGGTGGTACG
TAGTATCGGACAGGTAGAATACCTCTATGCCCTTAAGCGGGAAGAAGGTCGTCCCACAGCGCAAGTTCTGGCGGAAATAT
GTCCGGGACTTATCGCCGGGCTGCACTTTCCCAAACCCATGCGCTGGGGTGAACTGGAACTCCGTTTTGCCCGGCCCATT
CGCTGGTTACTGGCCCTGTTTGGTGAAGCTGTAGTACCCTTTGAACTGGCTAACTTGCAGTCCAACCGTTTTACCTATGG
TCATCGCTTCCTGTCCACAGGCGACCTGTCCATAGCCAATCCAGAAGATTACTTCACCAAAATTCGAGGTGCCTATGTAC
TGATTGACCCAGCTGAACGAAAGGAACTCATCTGGCAACAGGTGCAGGAACTGGCTACGGCTGAAGGTGGCGTGGTGGAA
AAGGACGAGGACCTGCTGGATGAAATCACCAACATCCTTGAGTGGCCCACGGCCCTTTGTGGCACCTTTGATGAGGATTA
TTTGAAGCTGCCCGGAGCTGTACTGGTTACTCCCATGCGGGAACATCAACGTTATTTTCCTGTGGTTAGCAATGAAGGAA
AACTATTAAATAAATTTATAGCGGTAAGAAACGGAACCAGGGCATATATAGAAATTGTAACTGCTGGTAATGAAAAGGTT
CTACGGGCCCGCTTAGCAGATGCGGCCTTCTTCTTTGAAGAAGACTTAAAACAACCCCTAGCCAGCAAAGTAAACGGTTT
ACAGAAAGTGGTGTTCCTAGAGGGTCTGGGCTCCATCGCTGATAAAGTGGACCGTATAGGCGCAATGGCAGACCACCTGG
CAGAAACACTGGGAGCAAATGAGGAACAACGGGAGAATATTCAACGGGGAGCCCTGCTGGCTAAAGCCGATCTAATTACC
AATATGGTCTATGAATTTCCCGAACTCCAAGGAGAAATGGGTCGTGAATATGCCCTGCGCAACGGTGAAGCCCCAGAAGT
TGCAGAAGCCATTTTTGAACATTATTTACCCCGTTTTGCAGGGGACCTGTTACCTGAGACCCTGGCAGGCAGTGTGCTGA
GTGTAGCTGATAAGATGGATTCTATCGTTGGGTGCTTTGCCATTGGTATTCAACCCACTGGTTCCCAGGATCCCTATGCA
CTTCGTCGGCAGGCCCTGGGAATATGCCACATGCTAATTGAAGGTAACATTCACCTGTCCCTCAGGGAACTGGTTCAGTG
GGCCTACCAGGGTTACCATGAAGGGGTAGAGTTAAAACAGGATTTAAACCAAGTAATCACGGAAATTGAAGAATTCTTTA
AACAGAGACTTAAAGGCATTCTCAACGATCGGGGTTTGTCCTATGACACCGTAGATGCTGTACTGACCGCAGGCTTTGAC
GACATAGCTGACGTTGTAGACAGAGGAATGGCTCTAGCTGCCTTCCGTGAATTGCCTGCTTTTGCAGCATTGATGACAGC
CTTTAACAGAGCCAATAACTTGGCCAAACATGCCACCACCACACAAGTACAGGAAGTTCACCTGGAGCATTCGGCAGAAC
AAGAGTTATACGGTCTGTTGACAAAACTTGAAGGGGAAGTAAGGCCCCTGCTGGAACAAAAGAATTATGCCTTAGCTTTA
CAAAAAATCGCCACTATTCAAAGCCCCCTCGATACTTTCTTTGAAAGCGTTATGGTTATGGTAGAAGATGAGGCGGTAAA
GACCAATCGGTTGGCCCTTCTGAAAAAGCTGGTTGGACTAAGTATGAATGTGGCTGATTTTAGCAAAATAGTGGTTGAAA
CAAAGTAG

Upstream 100 bases:

>100_bases
ATTGCCCGGGTAAGGCAGATGGCCAGGGCCTGCGCCCATGCCTATGTGGAGCAGCGTGAAAAATTAGGATTTCCTCTGTT
AAAGAAAGGGGGCAATATCA

Downstream 100 bases:

>100_bases
TATAAAAAAAGACAAAAATTTTTCTCAAAAAGTAGGATTTATTTAGTCGAATATAGTATATTATTGTAAGGGAATTCACT
ATCAATAGTATGTCACATTT

Product: glycine--tRNA ligase

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MAKDFLLEVGIEEMPARFLGPALTQLKEQTVKTLQELRIEYADIQTYGTPRRLVLYIKDLAENQAALEKEVKGPAKKAAF
DAAGNPTKAILGFTRSQGVSMEDLVVRSIGQVEYLYALKREEGRPTAQVLAEICPGLIAGLHFPKPMRWGELELRFARPI
RWLLALFGEAVVPFELANLQSNRFTYGHRFLSTGDLSIANPEDYFTKIRGAYVLIDPAERKELIWQQVQELATAEGGVVE
KDEDLLDEITNILEWPTALCGTFDEDYLKLPGAVLVTPMREHQRYFPVVSNEGKLLNKFIAVRNGTRAYIEIVTAGNEKV
LRARLADAAFFFEEDLKQPLASKVNGLQKVVFLEGLGSIADKVDRIGAMADHLAETLGANEEQRENIQRGALLAKADLIT
NMVYEFPELQGEMGREYALRNGEAPEVAEAIFEHYLPRFAGDLLPETLAGSVLSVADKMDSIVGCFAIGIQPTGSQDPYA
LRRQALGICHMLIEGNIHLSLRELVQWAYQGYHEGVELKQDLNQVITEIEEFFKQRLKGILNDRGLSYDTVDAVLTAGFD
DIADVVDRGMALAAFRELPAFAALMTAFNRANNLAKHATTTQVQEVHLEHSAEQELYGLLTKLEGEVRPLLEQKNYALAL
QKIATIQSPLDTFFESVMVMVEDEAVKTNRLALLKKLVGLSMNVADFSKIVVETK

Sequences:

>Translated_695_residues
MAKDFLLEVGIEEMPARFLGPALTQLKEQTVKTLQELRIEYADIQTYGTPRRLVLYIKDLAENQAALEKEVKGPAKKAAF
DAAGNPTKAILGFTRSQGVSMEDLVVRSIGQVEYLYALKREEGRPTAQVLAEICPGLIAGLHFPKPMRWGELELRFARPI
RWLLALFGEAVVPFELANLQSNRFTYGHRFLSTGDLSIANPEDYFTKIRGAYVLIDPAERKELIWQQVQELATAEGGVVE
KDEDLLDEITNILEWPTALCGTFDEDYLKLPGAVLVTPMREHQRYFPVVSNEGKLLNKFIAVRNGTRAYIEIVTAGNEKV
LRARLADAAFFFEEDLKQPLASKVNGLQKVVFLEGLGSIADKVDRIGAMADHLAETLGANEEQRENIQRGALLAKADLIT
NMVYEFPELQGEMGREYALRNGEAPEVAEAIFEHYLPRFAGDLLPETLAGSVLSVADKMDSIVGCFAIGIQPTGSQDPYA
LRRQALGICHMLIEGNIHLSLRELVQWAYQGYHEGVELKQDLNQVITEIEEFFKQRLKGILNDRGLSYDTVDAVLTAGFD
DIADVVDRGMALAAFRELPAFAALMTAFNRANNLAKHATTTQVQEVHLEHSAEQELYGLLTKLEGEVRPLLEQKNYALAL
QKIATIQSPLDTFFESVMVMVEDEAVKTNRLALLKKLVGLSMNVADFSKIVVETK
>Mature_694_residues
AKDFLLEVGIEEMPARFLGPALTQLKEQTVKTLQELRIEYADIQTYGTPRRLVLYIKDLAENQAALEKEVKGPAKKAAFD
AAGNPTKAILGFTRSQGVSMEDLVVRSIGQVEYLYALKREEGRPTAQVLAEICPGLIAGLHFPKPMRWGELELRFARPIR
WLLALFGEAVVPFELANLQSNRFTYGHRFLSTGDLSIANPEDYFTKIRGAYVLIDPAERKELIWQQVQELATAEGGVVEK
DEDLLDEITNILEWPTALCGTFDEDYLKLPGAVLVTPMREHQRYFPVVSNEGKLLNKFIAVRNGTRAYIEIVTAGNEKVL
RARLADAAFFFEEDLKQPLASKVNGLQKVVFLEGLGSIADKVDRIGAMADHLAETLGANEEQRENIQRGALLAKADLITN
MVYEFPELQGEMGREYALRNGEAPEVAEAIFEHYLPRFAGDLLPETLAGSVLSVADKMDSIVGCFAIGIQPTGSQDPYAL
RRQALGICHMLIEGNIHLSLRELVQWAYQGYHEGVELKQDLNQVITEIEEFFKQRLKGILNDRGLSYDTVDAVLTAGFDD
IADVVDRGMALAAFRELPAFAALMTAFNRANNLAKHATTTQVQEVHLEHSAEQELYGLLTKLEGEVRPLLEQKNYALALQ
KIATIQSPLDTFFESVMVMVEDEAVKTNRLALLKKLVGLSMNVADFSKIVVETK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=690, Percent_Identity=37.3913043478261, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_DESRM (A4J7C9)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113807.1
- ProteinModelPortal:   A4J7C9
- STRING:   A4J7C9
- GeneID:   4957139
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2472
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   A4J7C9
- ProtClustDB:   PRK01233
- BioCyc:   DRED349161:DRED_2472-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 77490; Mature: 77359

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDFLLEVGIEEMPARFLGPALTQLKEQTVKTLQELRIEYADIQTYGTPRRLVLYIKDL
CCCHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AENQAALEKEVKGPAKKAAFDAAGNPTKAILGFTRSQGVSMEDLVVRSIGQVEYLYALKR
HHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHHH
EEGRPTAQVLAEICPGLIAGLHFPKPMRWGELELRFARPIRWLLALFGEAVVPFELANLQ
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCC
SNRFTYGHRFLSTGDLSIANPEDYFTKIRGAYVLIDPAERKELIWQQVQELATAEGGVVE
CCCEEHHHHEEECCCCCCCCCHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCC
KDEDLLDEITNILEWPTALCGTFDEDYLKLPGAVLVTPMREHQRYFPVVSNEGKLLNKFI
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEECCHHHHHCCCCEECCCHHHHHHHH
AVRNGTRAYIEIVTAGNEKVLRARLADAAFFFEEDLKQPLASKVNGLQKVVFLEGLGSIA
HHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKVDRIGAMADHLAETLGANEEQRENIQRGALLAKADLITNMVYEFPELQGEMGREYALR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHC
NGEAPEVAEAIFEHYLPRFAGDLLPETLAGSVLSVADKMDSIVGCFAIGIQPTGSQDPYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LRRQALGICHMLIEGNIHLSLRELVQWAYQGYHEGVELKQDLNQVITEIEEFFKQRLKGI
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNDRGLSYDTVDAVLTAGFDDIADVVDRGMALAAFRELPAFAALMTAFNRANNLAKHATT
HHCCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQVQEVHLEHSAEQELYGLLTKLEGEVRPLLEQKNYALALQKIATIQSPLDTFFESVMVM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VEDEAVKTNRLALLKKLVGLSMNVADFSKIVVETK
HCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHEECC
>Mature Secondary Structure 
AKDFLLEVGIEEMPARFLGPALTQLKEQTVKTLQELRIEYADIQTYGTPRRLVLYIKDL
CCHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AENQAALEKEVKGPAKKAAFDAAGNPTKAILGFTRSQGVSMEDLVVRSIGQVEYLYALKR
HHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHHH
EEGRPTAQVLAEICPGLIAGLHFPKPMRWGELELRFARPIRWLLALFGEAVVPFELANLQ
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCC
SNRFTYGHRFLSTGDLSIANPEDYFTKIRGAYVLIDPAERKELIWQQVQELATAEGGVVE
CCCEEHHHHEEECCCCCCCCCHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCC
KDEDLLDEITNILEWPTALCGTFDEDYLKLPGAVLVTPMREHQRYFPVVSNEGKLLNKFI
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEECCHHHHHCCCCEECCCHHHHHHHH
AVRNGTRAYIEIVTAGNEKVLRARLADAAFFFEEDLKQPLASKVNGLQKVVFLEGLGSIA
HHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKVDRIGAMADHLAETLGANEEQRENIQRGALLAKADLITNMVYEFPELQGEMGREYALR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHC
NGEAPEVAEAIFEHYLPRFAGDLLPETLAGSVLSVADKMDSIVGCFAIGIQPTGSQDPYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LRRQALGICHMLIEGNIHLSLRELVQWAYQGYHEGVELKQDLNQVITEIEEFFKQRLKGI
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNDRGLSYDTVDAVLTAGFDDIADVVDRGMALAAFRELPAFAALMTAFNRANNLAKHATT
HHCCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQVQEVHLEHSAEQELYGLLTKLEGEVRPLLEQKNYALALQKIATIQSPLDTFFESVMVM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VEDEAVKTNRLALLKKLVGLSMNVADFSKIVVETK
HCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA