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The map label for this gene is dnaG [H]

Identifier: 134300302

GI number: 134300302

Start: 2681038

End: 2682852

Strand: Reverse

Name: dnaG [H]

Synonym: Dred_2463

Alternate gene names: 134300302

Gene position: 2682852-2681038 (Counterclockwise)

Preceding gene: 134300303

Following gene: 134300301

Centisome position: 74.36

GC content: 43.2

Gene sequence:

>1815_bases
ATGGCTGGAAGGATCCCGGATTCCGTTATTGAAGAAATCAGGCAAAGAACTGACATTGTCGAAGTCATTTCCAGGTTTAT
ACATGTGGAAAAACGCGGCAAGAACTATCTGGCCCTTTGCCCCTTTCACCAGGAAAAGACACCTTCCTTTAACATTAACC
CAGAGCGACAGATGTTTCACTGCTTTGGATGTGGTGTTGGGGGAAATGTCTTTAAATTTCTTATGATGGTGGAAGGTCTT
TCTTTCCCTGAATCTGTGAGGTCTTTGGGGACTAAACTTGGTATAGAGGTTCCCGAAGAAATTTCACCGAAAGAAAGGGT
TAAACAGGGAAAAAAGGATAGGGCTTTTAAAATATATGGATTAGCCAGGGATTTTTACCATTTTCTTCTTACCAAAGAAA
TTGGTATTGGGGCCATCAGATATCTGGAGTCCCGAAACCTGACTGATAAAACAAGGGAAACCTTTGAGTTGGGGTTTGCA
CCGTTAGGATGGGATTCATTAACCGGCTTTTTAACTGCCAAGGGAATTTCTGGCGAGGAACTCACAAAGCTGGGCCTGGT
ACAAAAAAAGGAATCTGGTGGTTATTACGATAGGTTTAGAAATCGAATCATGTTTCCCATCTGGGATAATCAGGGCCGGG
TAGTTGGCTTTGGCGGCAGAACCATGGGTGAGGATAATCCGAAGTACCTAAATTCCCCGGAAGGTGAATACTTCAATAAA
GGCCAGCTTCTTTATGGGTTGCATATTGCCCGTAAGGGCATTCGGGAACAGGGCTACGCTGTATTAATGGAAGGGTATAT
GGATGTTGTTTCCACCTACCAACATGGCGTTACCAATGCAGTGGCCTCATTGGGGACTGCTTTTACACGCGACCAGGGTA
AGATACTCATGATGTATACCCATGATGTTGTTATCGCCTATGATGCTGATACAGCTGGGATAAAAGCAGCAACCCGTGCA
GCAGATATTCTCCAAGAATTGGGTTGCCAGGTGAGGATCGCCACCATTGTAGGTGCAAAGGACCCAGATGAATATATTCA
AAGTCATGGTCTGGAAGGATGGCAAAGCATAATTGATCATGCAATGCCTTTAGTACAATACAAGCTTTTACAGGCTGCTC
AAAAGCATGGCCTACAAAATGCAGGGGCAAAGCAAAGAATACTCCGGGAGGTGCTACCCAATTTGGCTTCTGTGGCCAGT
GCCATTGAATTAGAAGAAACAGTTAAACAAGCGGCCACGGTTTTACAAGTAAATTGGGAGACCGTGCTGGAGGAAATTAA
AAAGTATAAAAGGAATTCCAGAAAAAAATCCCAAATAGAGGATAATTTTGCAAAAGAAAGCCATAATATATCGAGTGCAC
GGTATTTTGCTCCTGTTCGTCCCAAGGACGCGAGATCTAATGCAGAAGCAGGTGTTCTAAGGTTTGCTTTGGAAAATAAA
GAATACCTGGAAAGAATTCTACAGGAGTTGGGCAATGACTTTTTTCAAAATAATGTTTACCAGAGAATATTTGATGTCCA
CCTAGGGAACAAGGAACAAAATTCCGCCAGACTGTTGGATGAATTAGACGAATCCTCCCAAAGGATTGTCAGCAGTTTGC
TGGTGCAAGAAATTCCAGGGGAGAATATGGATCAAATACTTTCTGACTTTATTAATACCATAAAAAGAACCAGAGAAAAA
GCAAAACTTGATGATTTGTTGATGCAATTGGCTGCTGCGGAGAAAAGCGGAGATACCGAACGGGTAATCGAGCTGAGTCG
AAAGATTAATCTTACCATGAAATCCGAGGGGCCCGAAAGGGGAGTGGCAACATGA

Upstream 100 bases:

>100_bases
ATAAACTTTAAACTGTAGTTCGGAATTCCGGTTACAATCCAAAAAAAGGAAAGCACCCTGCATTGAACAAAAATAAGTAG
GGTAGTAAAAGGGTGAGTTT

Downstream 100 bases:

>100_bases
GGGACGAAGCGAAAGTAGAACAAATTAAAGAGTTAATCGAAAAAGGCAAAAAACGTGGGGTACTAACATATAACGAAATT
ATGGATACCCTGCAGGGCAC

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MAGRIPDSVIEEIRQRTDIVEVISRFIHVEKRGKNYLALCPFHQEKTPSFNINPERQMFHCFGCGVGGNVFKFLMMVEGL
SFPESVRSLGTKLGIEVPEEISPKERVKQGKKDRAFKIYGLARDFYHFLLTKEIGIGAIRYLESRNLTDKTRETFELGFA
PLGWDSLTGFLTAKGISGEELTKLGLVQKKESGGYYDRFRNRIMFPIWDNQGRVVGFGGRTMGEDNPKYLNSPEGEYFNK
GQLLYGLHIARKGIREQGYAVLMEGYMDVVSTYQHGVTNAVASLGTAFTRDQGKILMMYTHDVVIAYDADTAGIKAATRA
ADILQELGCQVRIATIVGAKDPDEYIQSHGLEGWQSIIDHAMPLVQYKLLQAAQKHGLQNAGAKQRILREVLPNLASVAS
AIELEETVKQAATVLQVNWETVLEEIKKYKRNSRKKSQIEDNFAKESHNISSARYFAPVRPKDARSNAEAGVLRFALENK
EYLERILQELGNDFFQNNVYQRIFDVHLGNKEQNSARLLDELDESSQRIVSSLLVQEIPGENMDQILSDFINTIKRTREK
AKLDDLLMQLAAAEKSGDTERVIELSRKINLTMKSEGPERGVAT

Sequences:

>Translated_604_residues
MAGRIPDSVIEEIRQRTDIVEVISRFIHVEKRGKNYLALCPFHQEKTPSFNINPERQMFHCFGCGVGGNVFKFLMMVEGL
SFPESVRSLGTKLGIEVPEEISPKERVKQGKKDRAFKIYGLARDFYHFLLTKEIGIGAIRYLESRNLTDKTRETFELGFA
PLGWDSLTGFLTAKGISGEELTKLGLVQKKESGGYYDRFRNRIMFPIWDNQGRVVGFGGRTMGEDNPKYLNSPEGEYFNK
GQLLYGLHIARKGIREQGYAVLMEGYMDVVSTYQHGVTNAVASLGTAFTRDQGKILMMYTHDVVIAYDADTAGIKAATRA
ADILQELGCQVRIATIVGAKDPDEYIQSHGLEGWQSIIDHAMPLVQYKLLQAAQKHGLQNAGAKQRILREVLPNLASVAS
AIELEETVKQAATVLQVNWETVLEEIKKYKRNSRKKSQIEDNFAKESHNISSARYFAPVRPKDARSNAEAGVLRFALENK
EYLERILQELGNDFFQNNVYQRIFDVHLGNKEQNSARLLDELDESSQRIVSSLLVQEIPGENMDQILSDFINTIKRTREK
AKLDDLLMQLAAAEKSGDTERVIELSRKINLTMKSEGPERGVAT
>Mature_603_residues
AGRIPDSVIEEIRQRTDIVEVISRFIHVEKRGKNYLALCPFHQEKTPSFNINPERQMFHCFGCGVGGNVFKFLMMVEGLS
FPESVRSLGTKLGIEVPEEISPKERVKQGKKDRAFKIYGLARDFYHFLLTKEIGIGAIRYLESRNLTDKTRETFELGFAP
LGWDSLTGFLTAKGISGEELTKLGLVQKKESGGYYDRFRNRIMFPIWDNQGRVVGFGGRTMGEDNPKYLNSPEGEYFNKG
QLLYGLHIARKGIREQGYAVLMEGYMDVVSTYQHGVTNAVASLGTAFTRDQGKILMMYTHDVVIAYDADTAGIKAATRAA
DILQELGCQVRIATIVGAKDPDEYIQSHGLEGWQSIIDHAMPLVQYKLLQAAQKHGLQNAGAKQRILREVLPNLASVASA
IELEETVKQAATVLQVNWETVLEEIKKYKRNSRKKSQIEDNFAKESHNISSARYFAPVRPKDARSNAEAGVLRFALENKE
YLERILQELGNDFFQNNVYQRIFDVHLGNKEQNSARLLDELDESSQRIVSSLLVQEIPGENMDQILSDFINTIKRTREKA
KLDDLLMQLAAAEKSGDTERVIELSRKINLTMKSEGPERGVAT

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=402, Percent_Identity=39.0547263681592, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68147; Mature: 68016

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRIPDSVIEEIRQRTDIVEVISRFIHVEKRGKNYLALCPFHQEKTPSFNINPERQMFH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHH
CFGCGVGGNVFKFLMMVEGLSFPESVRSLGTKLGIEVPEEISPKERVKQGKKDRAFKIYG
HCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCEEEEH
LARDFYHFLLTKEIGIGAIRYLESRNLTDKTRETFELGFAPLGWDSLTGFLTAKGISGEE
HHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHEECCCCHHH
LTKLGLVQKKESGGYYDRFRNRIMFPIWDNQGRVVGFGGRTMGEDNPKYLNSPEGEYFNK
HHHHHHHEECCCCCHHHHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
GQLLYGLHIARKGIREQGYAVLMEGYMDVVSTYQHGVTNAVASLGTAFTRDQGKILMMYT
CCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HDVVIAYDADTAGIKAATRAADILQELGCQVRIATIVGAKDPDEYIQSHGLEGWQSIIDH
CCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCHHHHHHHH
AMPLVQYKLLQAAQKHGLQNAGAKQRILREVLPNLASVASAIELEETVKQAATVLQVNWE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
TVLEEIKKYKRNSRKKSQIEDNFAKESHNISSARYFAPVRPKDARSNAEAGVLRFALENK
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHCCH
EYLERILQELGNDFFQNNVYQRIFDVHLGNKEQNSARLLDELDESSQRIVSSLLVQEIPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENMDQILSDFINTIKRTREKAKLDDLLMQLAAAEKSGDTERVIELSRKINLTMKSEGPER
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCCC
GVAT
CCCC
>Mature Secondary Structure 
AGRIPDSVIEEIRQRTDIVEVISRFIHVEKRGKNYLALCPFHQEKTPSFNINPERQMFH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHH
CFGCGVGGNVFKFLMMVEGLSFPESVRSLGTKLGIEVPEEISPKERVKQGKKDRAFKIYG
HCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCEEEEH
LARDFYHFLLTKEIGIGAIRYLESRNLTDKTRETFELGFAPLGWDSLTGFLTAKGISGEE
HHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHEECCCCHHH
LTKLGLVQKKESGGYYDRFRNRIMFPIWDNQGRVVGFGGRTMGEDNPKYLNSPEGEYFNK
HHHHHHHEECCCCCHHHHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
GQLLYGLHIARKGIREQGYAVLMEGYMDVVSTYQHGVTNAVASLGTAFTRDQGKILMMYT
CCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HDVVIAYDADTAGIKAATRAADILQELGCQVRIATIVGAKDPDEYIQSHGLEGWQSIIDH
CCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCHHHHHHHH
AMPLVQYKLLQAAQKHGLQNAGAKQRILREVLPNLASVASAIELEETVKQAATVLQVNWE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
TVLEEIKKYKRNSRKKSQIEDNFAKESHNISSARYFAPVRPKDARSNAEAGVLRFALENK
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHCCH
EYLERILQELGNDFFQNNVYQRIFDVHLGNKEQNSARLLDELDESSQRIVSSLLVQEIPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENMDQILSDFINTIKRTREKAKLDDLLMQLAAAEKSGDTERVIELSRKINLTMKSEGPER
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCCC
GVAT
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]