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The map label for this gene is sigA [H]

Identifier: 134300301

GI number: 134300301

Start: 2679938

End: 2681041

Strand: Reverse

Name: sigA [H]

Synonym: Dred_2462

Alternate gene names: 134300301

Gene position: 2681041-2679938 (Counterclockwise)

Preceding gene: 134300302

Following gene: 134300300

Centisome position: 74.31

GC content: 43.48

Gene sequence:

>1104_bases
ATGAGGGACGAAGCGAAAGTAGAACAAATTAAAGAGTTAATCGAAAAAGGCAAAAAACGTGGGGTACTAACATATAACGA
AATTATGGATACCCTGCAGGGCACCGAGTTGTCGCCAGATCAAATTGACGACATTTATGAAAAGATTTCTGCTATGGGTA
TTGAAGTTGTCCCCTTAGCCAGTGATTTGGAGCCTCTGGAAGTAGATGACTCAGTAGAAAATTCAACCGAGGAAGAGGTT
GAGGTTGAAGTTGATTTAAGTATTCCCGAGGGTATTGGTATCGATGACCCCGTTAGAATGTACCTAAAGGAAATTGGCCG
GGTTCCCCTACTATCGCCCGAAGAAGAAGTAGACTTGGCTAAAAGGATGGAGCAAGGAGAAGAAGAAGCTAAACGACGTC
TTGCGGAGGCTAATTTACGACTGGTTGTTAGTATTGCCAAGCGCTACGTGGGCAGGGGTATGCTTTTCTTGGATTTAATC
CAGGAAGGAAATTTAGGTCTTATTAAAGCGGTAGAAAAATTTGATTATCGAAAAGGTTATAAATTCAGTACCTATGCCAC
CTGGTGGATTCGGCAGGCCATTACCCGGGCCATTGCAGATCAGGCCCGTACCATCCGTATCCCTGTACATATGGTGGAAA
CCATAAATAAATTGATTCGGGTTTCTCGCCAACTGCTACAGGAACTGGGAAGGGAACCCTCCCCTGAAGAAATTGCCAAA
GAGATGGATATTTCTGGTGAAAAAGTTCGAGAAATCTTGAAAATTGCTCAGGAGCCTGTATCACTGGAAACGCCCATTGG
TGAAGAAGAAGATTCACACCTGGGTGATTTTATTGAAGATGCAGATGCCAGGGCACCAGCAGAAGAAGCTTCCTTTACAC
TTTTGCGCGAACAATTAGATGAAGTTCTTAAAACCTTAACGGATCGTGAGCAAAAGGTTCTTCGCCTGCGTTTTGGTTTG
GACGATGGTCGTGCTAGAACATTGGAAGAAGTGGGACAAAAATTTGGCGTTACTAGAGAACGTATTCGTCAAATTGAAGC
AAAGACGTTACGGAAACTGCGACATCCAAGCCGTAGTAAGAAATTAAAGGATTACCTGGATTAA

Upstream 100 bases:

>100_bases
CTGCTGCGGAGAAAAGCGGAGATACCGAACGGGTAATCGAGCTGAGTCGAAAGATTAATCTTACCATGAAATCCGAGGGG
CCCGAAAGGGGAGTGGCAAC

Downstream 100 bases:

>100_bases
TAAATAATTGCGAATAACCAAGTAAAAAATACAATAAAAAATGAAATTATGGGTTGACCTGACATAGTTGTTTTAGTATA
ATAACTTTCGTGGTGATGCT

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MRDEAKVEQIKELIEKGKKRGVLTYNEIMDTLQGTELSPDQIDDIYEKISAMGIEVVPLASDLEPLEVDDSVENSTEEEV
EVEVDLSIPEGIGIDDPVRMYLKEIGRVPLLSPEEEVDLAKRMEQGEEEAKRRLAEANLRLVVSIAKRYVGRGMLFLDLI
QEGNLGLIKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLLQELGREPSPEEIAK
EMDISGEKVREILKIAQEPVSLETPIGEEEDSHLGDFIEDADARAPAEEASFTLLREQLDEVLKTLTDREQKVLRLRFGL
DDGRARTLEEVGQKFGVTRERIRQIEAKTLRKLRHPSRSKKLKDYLD

Sequences:

>Translated_367_residues
MRDEAKVEQIKELIEKGKKRGVLTYNEIMDTLQGTELSPDQIDDIYEKISAMGIEVVPLASDLEPLEVDDSVENSTEEEV
EVEVDLSIPEGIGIDDPVRMYLKEIGRVPLLSPEEEVDLAKRMEQGEEEAKRRLAEANLRLVVSIAKRYVGRGMLFLDLI
QEGNLGLIKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLLQELGREPSPEEIAK
EMDISGEKVREILKIAQEPVSLETPIGEEEDSHLGDFIEDADARAPAEEASFTLLREQLDEVLKTLTDREQKVLRLRFGL
DDGRARTLEEVGQKFGVTRERIRQIEAKTLRKLRHPSRSKKLKDYLD
>Mature_367_residues
MRDEAKVEQIKELIEKGKKRGVLTYNEIMDTLQGTELSPDQIDDIYEKISAMGIEVVPLASDLEPLEVDDSVENSTEEEV
EVEVDLSIPEGIGIDDPVRMYLKEIGRVPLLSPEEEVDLAKRMEQGEEEAKRRLAEANLRLVVSIAKRYVGRGMLFLDLI
QEGNLGLIKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLLQELGREPSPEEIAK
EMDISGEKVREILKIAQEPVSLETPIGEEEDSHLGDFIEDADARAPAEEASFTLLREQLDEVLKTLTDREQKVLRLRFGL
DDGRARTLEEVGQKFGVTRERIRQIEAKTLRKLRHPSRSKKLKDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=260, Percent_Identity=65.3846153846154, Blast_Score=351, Evalue=4e-98,
Organism=Escherichia coli, GI1789098, Length=268, Percent_Identity=45.5223880597015, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=30.1470588235294, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=29.2079207920792, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 42037; Mature: 42037

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDEAKVEQIKELIEKGKKRGVLTYNEIMDTLQGTELSPDQIDDIYEKISAMGIEVVPLA
CCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECC
SDLEPLEVDDSVENSTEEEVEVEVDLSIPEGIGIDDPVRMYLKEIGRVPLLSPEEEVDLA
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
KRMEQGEEEAKRRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYRKGY
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCC
KFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLLQELGREPSPEEIAK
CCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EMDISGEKVREILKIAQEPVSLETPIGEEEDSHLGDFIEDADARAPAEEASFTLLREQLD
HHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EVLKTLTDREQKVLRLRFGLDDGRARTLEEVGQKFGVTRERIRQIEAKTLRKLRHPSRSK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHH
KLKDYLD
HHHHHCC
>Mature Secondary Structure
MRDEAKVEQIKELIEKGKKRGVLTYNEIMDTLQGTELSPDQIDDIYEKISAMGIEVVPLA
CCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECC
SDLEPLEVDDSVENSTEEEVEVEVDLSIPEGIGIDDPVRMYLKEIGRVPLLSPEEEVDLA
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
KRMEQGEEEAKRRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYRKGY
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCC
KFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLLQELGREPSPEEIAK
CCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EMDISGEKVREILKIAQEPVSLETPIGEEEDSHLGDFIEDADARAPAEEASFTLLREQLD
HHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EVLKTLTDREQKVLRLRFGLDDGRARTLEEVGQKFGVTRERIRQIEAKTLRKLRHPSRSK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHH
KLKDYLD
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]