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The map label for this gene is purH [H]

Identifier: 134300201

GI number: 134300201

Start: 2585601

End: 2587142

Strand: Reverse

Name: purH [H]

Synonym: Dred_2362

Alternate gene names: 134300201

Gene position: 2587142-2585601 (Counterclockwise)

Preceding gene: 134300202

Following gene: 134300200

Centisome position: 71.7

GC content: 50.32

Gene sequence:

>1542_bases
ATGAAAATCAAACGCGCTTTAATTAGTGTTTCCGATAAAACCGGTCTGGTGGATTTTGCCAGGGGGTTGGCGAAACTGGG
AGTGGAGATTATCTCCACCGGCGGCACCGCAAAAGTCCTGCGGGAGGCCAGTGTACCGGTAATTTATATATCAGATGTTA
CCGGTTTTCCGGAAATTTTGGATGGCCGGGTTAAAACCCTGCATCCCAATGTTCATGGTGGTATTTTAGCCATGCGGACC
GAAGAACATCTTAGCCAACTTGAAGAACATAACATTACACCCATAGACCTGGTGGTGGTAAACCTTTATCCTTTTCGTGA
AACCATTGCCAAACCTGAGGTGACCTTGGAAGATGCCATAGAAAATATTGATATTGGTGGGCCCACTATGGTACGGTCCG
CGGCTAAGAACCATGCCTATGTGGGGGTTGTGGTCAATCCCCAGCGTTACGGTCAGGTTCTGGAAGCCTTAGGGGAAAAG
GGAGAACTTACAGGAGATCAGCGTTTGGAGTTGGCCAGGGAAGCCTTTGCCCACACGGCCAGTTATGATACAGCCATTGC
CAATTATCTGGGCAGCGTAAGCCAAGCAGAGGCATTTCGTCCTGAGTTTAACTTTTCTTTAGTCAAGGTCCAGGACTTGC
GCTATGGGGAAAACCCGCACCAGAGGGCTGCCTTTTACAAAGATCCCGGCATTACTGGACCCTGTATTGCCAATGCCGTT
CAAATTCATGGGAAAGAACTTTCCTATAATAATATCCTGGATGCCAACAGCGCCCTGGAACTGGTTCGAGAATTTAGTGA
ACCCAGTGCAGTTATCATTAAACATAATAATCCCTGTGGTACCGCCACTGCAGAGGATTTGCCAGCGGCTTACCGTAAGG
CCTTTGCAGCGGACACTGTTTCTGCCTACGGGGGTATTGTGGCGGTGAACCGGACCCTTGATGGAGATACGGCCGCTGAA
ATGGCTAAGATCTTTTTGGAAGCGGTTATCGCACCGGACTTTACTACCGAAGCTTTGGACATTTTGACCAAGAAAGCCAA
TCTTAGGTTACTGAAAACCGGGGAACTAACGGAGCAAACCAATGATGTGTTAGAGGTCCGGAAGGTGAACGGGGGACTTT
TGGTCCAGCAGGCAGATCGGCGGGTGTTGAACATGGATGAGGTGAAGGTTGTAACCGAACGCCAACCCTCGCCGCAAGAA
TTAAAGGAATTAATCTTTGCCATGACGGTGGTTAAGCATGTGAAGTCCAACGCCATTGTTCTTTCTAAGGATGGACAGAT
TATTGGTGTTGGGGCAGGCCAGATGAATCGGGTTGGCTCCGCCCGCATTGCCTTTGAACAGGCCGGCGAAGGGGCCCGGG
GGGCTGTCATGGCCAGTGATGCCTTCTTCCCCTTCCGGGACACCATAGATGAAGCGGCCAAAGCCGGGGTGAAAGCCGTT
ATTCAACCCGGCGGTTCCATTCGGGATGAAGAATCCATTCAAGCTTGTAACGAGCACGGCATTGCCATGGTCTTTACCGG
CATGCGGCATTTTAAGCACTAG

Upstream 100 bases:

>100_bases
GCTGCAGGTTGTGGGGCGGAAAGTATATATTAAGAAATGAAGGGCAGATCGGTAATGATGGTTTGCCCATAACCCATTAA
GTGAAAGGCGTGGTAAGGAA

Downstream 100 bases:

>100_bases
AAGAAGCAGGAGTGGTCTTTAAATGAAAATTTTAGTGGTTGGCGGTGGCGGGCGGGAACACGCCTTGGTCTGGAAGCTCA
GACAAAGCCCCCGCGTGAAG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MKIKRALISVSDKTGLVDFARGLAKLGVEIISTGGTAKVLREASVPVIYISDVTGFPEILDGRVKTLHPNVHGGILAMRT
EEHLSQLEEHNITPIDLVVVNLYPFRETIAKPEVTLEDAIENIDIGGPTMVRSAAKNHAYVGVVVNPQRYGQVLEALGEK
GELTGDQRLELAREAFAHTASYDTAIANYLGSVSQAEAFRPEFNFSLVKVQDLRYGENPHQRAAFYKDPGITGPCIANAV
QIHGKELSYNNILDANSALELVREFSEPSAVIIKHNNPCGTATAEDLPAAYRKAFAADTVSAYGGIVAVNRTLDGDTAAE
MAKIFLEAVIAPDFTTEALDILTKKANLRLLKTGELTEQTNDVLEVRKVNGGLLVQQADRRVLNMDEVKVVTERQPSPQE
LKELIFAMTVVKHVKSNAIVLSKDGQIIGVGAGQMNRVGSARIAFEQAGEGARGAVMASDAFFPFRDTIDEAAKAGVKAV
IQPGGSIRDEESIQACNEHGIAMVFTGMRHFKH

Sequences:

>Translated_513_residues
MKIKRALISVSDKTGLVDFARGLAKLGVEIISTGGTAKVLREASVPVIYISDVTGFPEILDGRVKTLHPNVHGGILAMRT
EEHLSQLEEHNITPIDLVVVNLYPFRETIAKPEVTLEDAIENIDIGGPTMVRSAAKNHAYVGVVVNPQRYGQVLEALGEK
GELTGDQRLELAREAFAHTASYDTAIANYLGSVSQAEAFRPEFNFSLVKVQDLRYGENPHQRAAFYKDPGITGPCIANAV
QIHGKELSYNNILDANSALELVREFSEPSAVIIKHNNPCGTATAEDLPAAYRKAFAADTVSAYGGIVAVNRTLDGDTAAE
MAKIFLEAVIAPDFTTEALDILTKKANLRLLKTGELTEQTNDVLEVRKVNGGLLVQQADRRVLNMDEVKVVTERQPSPQE
LKELIFAMTVVKHVKSNAIVLSKDGQIIGVGAGQMNRVGSARIAFEQAGEGARGAVMASDAFFPFRDTIDEAAKAGVKAV
IQPGGSIRDEESIQACNEHGIAMVFTGMRHFKH
>Mature_513_residues
MKIKRALISVSDKTGLVDFARGLAKLGVEIISTGGTAKVLREASVPVIYISDVTGFPEILDGRVKTLHPNVHGGILAMRT
EEHLSQLEEHNITPIDLVVVNLYPFRETIAKPEVTLEDAIENIDIGGPTMVRSAAKNHAYVGVVVNPQRYGQVLEALGEK
GELTGDQRLELAREAFAHTASYDTAIANYLGSVSQAEAFRPEFNFSLVKVQDLRYGENPHQRAAFYKDPGITGPCIANAV
QIHGKELSYNNILDANSALELVREFSEPSAVIIKHNNPCGTATAEDLPAAYRKAFAADTVSAYGGIVAVNRTLDGDTAAE
MAKIFLEAVIAPDFTTEALDILTKKANLRLLKTGELTEQTNDVLEVRKVNGGLLVQQADRRVLNMDEVKVVTERQPSPQE
LKELIFAMTVVKHVKSNAIVLSKDGQIIGVGAGQMNRVGSARIAFEQAGEGARGAVMASDAFFPFRDTIDEAAKAGVKAV
IQPGGSIRDEESIQACNEHGIAMVFTGMRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=473, Percent_Identity=38.4778012684989, Blast_Score=284, Evalue=1e-76,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=53.9047619047619, Blast_Score=548, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71985564, Length=479, Percent_Identity=37.160751565762, Blast_Score=271, Evalue=9e-73,
Organism=Caenorhabditis elegans, GI71985574, Length=317, Percent_Identity=29.3375394321767, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=55, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323056, Length=470, Percent_Identity=37.6595744680851, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6323768, Length=471, Percent_Identity=36.5180467091295, Blast_Score=284, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24649832, Length=474, Percent_Identity=38.8185654008439, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55520; Mature: 55520

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKRALISVSDKTGLVDFARGLAKLGVEIISTGGTAKVLREASVPVIYISDVTGFPEIL
CCCHHHHEECCCCCCHHHHHHHHHHHCHHEEECCCHHHHHHHCCCCEEEEECCCCCHHHH
DGRVKTLHPNVHGGILAMRTEEHLSQLEEHNITPIDLVVVNLYPFRETIAKPEVTLEDAI
CCCEEEECCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEEEEECHHHHHCCCCCCHHHHH
ENIDIGGPTMVRSAAKNHAYVGVVVNPQRYGQVLEALGEKGELTGDQRLELAREAFAHTA
HHCCCCCHHHHHHHHCCCEEEEEEECHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
SYDTAIANYLGSVSQAEAFRPEFNFSLVKVQDLRYGENPHQRAAFYKDPGITGPCIANAV
HHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEECCCCCCHHHCCCEECCCCCCCHHHHHHH
QIHGKELSYNNILDANSALELVREFSEPSAVIIKHNNPCGTATAEDLPAAYRKAFAADTV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
SAYGGIVAVNRTLDGDTAAEMAKIFLEAVIAPDFTTEALDILTKKANLRLLKTGELTEQT
HHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCHHHH
NDVLEVRKVNGGLLVQQADRRVLNMDEVKVVTERQPSPQELKELIFAMTVVKHVKSNAIV
HHHHHHEECCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEE
LSKDGQIIGVGAGQMNRVGSARIAFEQAGEGARGAVMASDAFFPFRDTIDEAAKAGVKAV
EECCCCEEEECCCCCCCCCCCEEEHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEE
IQPGGSIRDEESIQACNEHGIAMVFTGMRHFKH
ECCCCCCCCHHHHHHHHCCCEEEEEECHHHHCC
>Mature Secondary Structure
MKIKRALISVSDKTGLVDFARGLAKLGVEIISTGGTAKVLREASVPVIYISDVTGFPEIL
CCCHHHHEECCCCCCHHHHHHHHHHHCHHEEECCCHHHHHHHCCCCEEEEECCCCCHHHH
DGRVKTLHPNVHGGILAMRTEEHLSQLEEHNITPIDLVVVNLYPFRETIAKPEVTLEDAI
CCCEEEECCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEEEEECHHHHHCCCCCCHHHHH
ENIDIGGPTMVRSAAKNHAYVGVVVNPQRYGQVLEALGEKGELTGDQRLELAREAFAHTA
HHCCCCCHHHHHHHHCCCEEEEEEECHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
SYDTAIANYLGSVSQAEAFRPEFNFSLVKVQDLRYGENPHQRAAFYKDPGITGPCIANAV
HHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEECCCCCCHHHCCCEECCCCCCCHHHHHHH
QIHGKELSYNNILDANSALELVREFSEPSAVIIKHNNPCGTATAEDLPAAYRKAFAADTV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
SAYGGIVAVNRTLDGDTAAEMAKIFLEAVIAPDFTTEALDILTKKANLRLLKTGELTEQT
HHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCHHHH
NDVLEVRKVNGGLLVQQADRRVLNMDEVKVVTERQPSPQELKELIFAMTVVKHVKSNAIV
HHHHHHEECCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEE
LSKDGQIIGVGAGQMNRVGSARIAFEQAGEGARGAVMASDAFFPFRDTIDEAAKAGVKAV
EECCCCEEEECCCCCCCCCCCEEEHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEE
IQPGGSIRDEESIQACNEHGIAMVFTGMRHFKH
ECCCCCCCCHHHHHHHHCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA