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The map label for this gene is purD [H]

Identifier: 134300200

GI number: 134300200

Start: 2584310

End: 2585578

Strand: Reverse

Name: purD [H]

Synonym: Dred_2361

Alternate gene names: 134300200

Gene position: 2585578-2584310 (Counterclockwise)

Preceding gene: 134300201

Following gene: 134300199

Centisome position: 71.66

GC content: 52.4

Gene sequence:

>1269_bases
ATGAAAATTTTAGTGGTTGGCGGTGGCGGGCGGGAACACGCCTTGGTCTGGAAGCTCAGACAAAGCCCCCGCGTGAAGCA
AATATACTGCGCCCCAGGTAATGCGGGTATCAGCCAGATTGCTGCCTGCATCAATGTTCGTGCAGAGGATACCCAGGGAT
TGCTGAATTTCGCTAAAAAAGAACAGATTGACCTGACAGTGGTGGGGCCGGAAATCCCTTTGACCAATGGTATTGTGGAT
CTGTTCCAGCAGGAAGGTCTTAAAATTTTTGGTCCCTCCAAGGCTGCTGCGGAAATAGAAGGCAGTAAAGCCCTGGCTAA
AGATATTATGGCTAAATACAACATCCCCACTGCCCGTTATGCAACCTTTGAAGATGCTGAGGAAGCAAGGGAGTATGTAA
AGAAATATGGTGCACCCTGTGTCATTAAGGCCGATGGCTTAGCTGCGGGTAAGGGTGTTATCGTAGCAATGGATGATGAA
ACAGCTCTGGCAGCGGTGGATATCATCATGGCAGAAAGGGCCTTTGGCAAGGCTGGGGATCGTGTGGTGATAGAAGAGTT
TTTACAGGGTGAAGAAGTAAGCATTCTAGCCTTTGTGGATGGAGAAACCATTGTACCGATGGTAGCTTCCCAGGACCATA
AGAGAGTGGGGGATCAGGATACTGGCCCCAACACCGGGGGCATGGGTGCCTATGCTCCGGCACCAGTTTATACGTCGGAA
CTGGCCAAGCTAGTGGAGCGGGATATTTTATACCCGACGGTGAAGGCCTTGGCTTCGGAAGGTCGGACCTACCGGGGTGT
GCTCTATGCAGGGCTCATGGTAACACCCGAGGGGCCGAAGGTGCTAGAATTTAATGCCCGCTTCGGTGATCCGGAAACCC
AGCCGGTATTAAGCCTGCTGGAGACAGACCTAGTGGATATTATGGAAGCCATCATGGAAGACAGGTTGGCCCAACAATCG
GTGATGTGGAAGGATGAAGCGGCTGTTTGTGTGGTTATGGCAGCCAAGGGCTACCCCGGAGAGTACGAAAAGGATATGGC
AATAACTGGCTTCGATCAGGTCCCAGCAGGCGTAGAAGTATTCCACGCCGGTACCACCCTGCGGCAAGGCGAGGTTGTTG
TGTCCGGCGGCAGAGTTCTGGGTGTAACCGCCACTGGCCGAGACATCTCCAAGGCCGTCGAACTGGCCTATCAAGGAGTT
AAGGCTATCCATTTTGCTGGAGCCCACTACAGATCGGATATTGGACAGAGGGCTTTAAACAGGGAGTAG

Upstream 100 bases:

>100_bases
GATGAAGAATCCATTCAAGCTTGTAACGAGCACGGCATTGCCATGGTCTTTACCGGCATGCGGCATTTTAAGCACTAGAA
GAAGCAGGAGTGGTCTTTAA

Downstream 100 bases:

>100_bases
GTCTAGAGCATAGGAAATTGTGTTAAGTGTCACGTTAACACCTGCCAAACAACTTACCGGAGCACCAGAGAGAAATTGGG
GGGCGCGTCCAGGACGGACG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKILVVGGGGREHALVWKLRQSPRVKQIYCAPGNAGISQIAACINVRAEDTQGLLNFAKKEQIDLTVVGPEIPLTNGIVD
LFQQEGLKIFGPSKAAAEIEGSKALAKDIMAKYNIPTARYATFEDAEEAREYVKKYGAPCVIKADGLAAGKGVIVAMDDE
TALAAVDIIMAERAFGKAGDRVVIEEFLQGEEVSILAFVDGETIVPMVASQDHKRVGDQDTGPNTGGMGAYAPAPVYTSE
LAKLVERDILYPTVKALASEGRTYRGVLYAGLMVTPEGPKVLEFNARFGDPETQPVLSLLETDLVDIMEAIMEDRLAQQS
VMWKDEAAVCVVMAAKGYPGEYEKDMAITGFDQVPAGVEVFHAGTTLRQGEVVVSGGRVLGVTATGRDISKAVELAYQGV
KAIHFAGAHYRSDIGQRALNRE

Sequences:

>Translated_422_residues
MKILVVGGGGREHALVWKLRQSPRVKQIYCAPGNAGISQIAACINVRAEDTQGLLNFAKKEQIDLTVVGPEIPLTNGIVD
LFQQEGLKIFGPSKAAAEIEGSKALAKDIMAKYNIPTARYATFEDAEEAREYVKKYGAPCVIKADGLAAGKGVIVAMDDE
TALAAVDIIMAERAFGKAGDRVVIEEFLQGEEVSILAFVDGETIVPMVASQDHKRVGDQDTGPNTGGMGAYAPAPVYTSE
LAKLVERDILYPTVKALASEGRTYRGVLYAGLMVTPEGPKVLEFNARFGDPETQPVLSLLETDLVDIMEAIMEDRLAQQS
VMWKDEAAVCVVMAAKGYPGEYEKDMAITGFDQVPAGVEVFHAGTTLRQGEVVVSGGRVLGVTATGRDISKAVELAYQGV
KAIHFAGAHYRSDIGQRALNRE
>Mature_422_residues
MKILVVGGGGREHALVWKLRQSPRVKQIYCAPGNAGISQIAACINVRAEDTQGLLNFAKKEQIDLTVVGPEIPLTNGIVD
LFQQEGLKIFGPSKAAAEIEGSKALAKDIMAKYNIPTARYATFEDAEEAREYVKKYGAPCVIKADGLAAGKGVIVAMDDE
TALAAVDIIMAERAFGKAGDRVVIEEFLQGEEVSILAFVDGETIVPMVASQDHKRVGDQDTGPNTGGMGAYAPAPVYTSE
LAKLVERDILYPTVKALASEGRTYRGVLYAGLMVTPEGPKVLEFNARFGDPETQPVLSLLETDLVDIMEAIMEDRLAQQS
VMWKDEAAVCVVMAAKGYPGEYEKDMAITGFDQVPAGVEVFHAGTTLRQGEVVVSGGRVLGVTATGRDISKAVELAYQGV
KAIHFAGAHYRSDIGQRALNRE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=49.4089834515366, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=49.4089834515366, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=49.4089834515366, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=49.4089834515366, Blast_Score=406, Evalue=1e-113,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=54.8009367681499, Blast_Score=451, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=40.5660377358491, Blast_Score=302, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6321203, Length=442, Percent_Identity=44.1176470588235, Blast_Score=355, Evalue=1e-98,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=46.046511627907, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=46.046511627907, Blast_Score=356, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45251; Mature: 45251

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVVGGGGREHALVWKLRQSPRVKQIYCAPGNAGISQIAACINVRAEDTQGLLNFAKK
CEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
EQIDLTVVGPEIPLTNGIVDLFQQEGLKIFGPSKAAAEIEGSKALAKDIMAKYNIPTARY
CCEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCHHHCCCHHHHHHHHHHHCCCCCCEE
ATFEDAEEAREYVKKYGAPCVIKADGLAAGKGVIVAMDDETALAAVDIIMAERAFGKAGD
ECCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCC
RVVIEEFLQGEEVSILAFVDGETIVPMVASQDHKRVGDQDTGPNTGGMGAYAPAPVYTSE
HHHHHHHHCCCCEEEEEEECCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHH
LAKLVERDILYPTVKALASEGRTYRGVLYAGLMVTPEGPKVLEFNARFGDPETQPVLSLL
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHH
ETDLVDIMEAIMEDRLAQQSVMWKDEAAVCVVMAAKGYPGEYEKDMAITGFDQVPAGVEV
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCEEE
FHAGTTLRQGEVVVSGGRVLGVTATGRDISKAVELAYQGVKAIHFAGAHYRSDIGQRALN
EECCCEEECCCEEEECCEEEEEEECCHHHHHHHHHHHHCHHHEEECCCHHHHHHHHHHHC
RE
CC
>Mature Secondary Structure
MKILVVGGGGREHALVWKLRQSPRVKQIYCAPGNAGISQIAACINVRAEDTQGLLNFAKK
CEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
EQIDLTVVGPEIPLTNGIVDLFQQEGLKIFGPSKAAAEIEGSKALAKDIMAKYNIPTARY
CCEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCHHHCCCHHHHHHHHHHHCCCCCCEE
ATFEDAEEAREYVKKYGAPCVIKADGLAAGKGVIVAMDDETALAAVDIIMAERAFGKAGD
ECCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCC
RVVIEEFLQGEEVSILAFVDGETIVPMVASQDHKRVGDQDTGPNTGGMGAYAPAPVYTSE
HHHHHHHHCCCCEEEEEEECCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHH
LAKLVERDILYPTVKALASEGRTYRGVLYAGLMVTPEGPKVLEFNARFGDPETQPVLSLL
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHH
ETDLVDIMEAIMEDRLAQQSVMWKDEAAVCVVMAAKGYPGEYEKDMAITGFDQVPAGVEV
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCEEE
FHAGTTLRQGEVVVSGGRVLGVTATGRDISKAVELAYQGVKAIHFAGAHYRSDIGQRALN
EECCCEEECCCEEEECCEEEEEEECCHHHHHHHHHHHHCHHHEEECCCHHHHHHHHHHHC
RE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]