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The map label for this gene is gatB [H]

Identifier: 134300179

GI number: 134300179

Start: 2558367

End: 2559806

Strand: Reverse

Name: gatB [H]

Synonym: Dred_2340

Alternate gene names: 134300179

Gene position: 2559806-2558367 (Counterclockwise)

Preceding gene: 134300180

Following gene: 134300178

Centisome position: 70.95

GC content: 45.42

Gene sequence:

>1440_bases
ATGACACAACAGTATGAAACTGTCATCGGTATTGAAGTACACGTTGAACTGAAAACCAACACAAAGATTTTTTGTAACTC
CACCACCGAATTTGGTGGGGATCCAAACCATCATACCTGTCCCGTGTGTCTGGGACTCCCCGGTACCCTACCGGTGTTAA
ACAAAAAAGTGGTAGAATATGCGGTGCGGGCATCCCTGGCACTGAACTGTCAGGTAGCCAACTTCTCCAAGTTTGACCGT
AAAAATTACTATTATCCGGATTTACCGAAAAACTATCAGATTTCCCAGTACGATCTGCCCATTGCTGAGCATGGTTATCT
GGATATTGTGGCGGATGGACAGCCAAAACGCATTGGCATCACCCGTTTACATATGGAAGAGGATGCAGGTAAACTGGTAC
ACCAGGGGAATATCGTAACTACTCCTTACTCCCTGGTGGACTATAACCGTACGGGTGTACCTTTAATTGAAATTGTTTCT
GAACCGGATATGAGATCATCAGAAGAAGCCAGGGCCTATGTGGAAAAGCTGAAGGCAATTGTCCAGTACACTGGTGTATC
TGATTGCCGGATGGAAGAGGGCAGCCTGCGCTGCGACGTAAACGTGTCGGTTCGTCCAGTGGGGCAAAAGGAATTCGGGA
CCAAGGCTGAGATTAAAAATCTTAACTCCTTTAGAGCATTACAGCGGGCTCTGGATTTTGAGGTTGAAAGACAAATAGGT
GTGTTGGAATCCGGCGGAAGGATTATTCAGGAAACCCGCACCTGGGATGAGGCAAGGGGTATTACCGTTTCCATGCGCAG
TAAGGAAGAGGCCCATGACTACCGCTATTTCCCAGATCCAGATCTGGTACCTCTGGTGTTGGATAACCAGTGGATTGATT
TTATTCGTGAATCACTGCCAGAATTACCAGATCAGCGTCGGAGTCGGTATATTACAGAATATGGTTTACCGGAATATGAT
GCCGCGCTATTAACCTTAACCAAAGAAATGTCCGACTACTTTGAAGAAGTTATGGAGCATTATAATAATCCCAAAGCTAT
TAGCAACTGGATCATGGGTGAACTGAGCCGTCTGCTTAATGCTGGTGGAATTGAGATTACCGCTTGTAAAATAAAGCCTG
CCCAGTTGGCGGAAATGTTAAAACTATTGGATAAAGGGACCATTAGTGGCAAGATTGCCAAAGCCGTTTTTGAAGAAATG
TTTGCCTCTGGTAAAGACCCGGAAGCAATTGTAAAAGAAAAGGGTCTGGTCCAGATCTCCGATGAGGGTGCCATTGCTTC
TATTGTAGATGAAGTGATTGCTGCCAATCCAAAATCAGTACAGGATTATCAAGCAGGTAAAACCCAGGCCATTGGCTTCC
TGGTGGGCCAAGTCATGAAAGCCACCAAGGGTAAAGCGAACCCAGAGATGGTTAATAAAATGCTGCGGGAAAAATTATAA

Upstream 100 bases:

>100_bases
CAAAGCCTTTGATGAAGGAACTCTTTTGAGAATTGCCTATACCTTAGAGCAAAATACGGAATATACAAGGCTTCGGCCTG
AGATATAGGGGGTGGAGCAG

Downstream 100 bases:

>100_bases
GCAAAAAAGATTGTTTTCACAGACAGTGGAAACAATCTTTTTTACATGGATAAATATCATAAAGTTGTACAATAAATTAA
ACATTTTATAAAGTGTCCAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTQQYETVIGIEVHVELKTNTKIFCNSTTEFGGDPNHHTCPVCLGLPGTLPVLNKKVVEYAVRASLALNCQVANFSKFDR
KNYYYPDLPKNYQISQYDLPIAEHGYLDIVADGQPKRIGITRLHMEEDAGKLVHQGNIVTTPYSLVDYNRTGVPLIEIVS
EPDMRSSEEARAYVEKLKAIVQYTGVSDCRMEEGSLRCDVNVSVRPVGQKEFGTKAEIKNLNSFRALQRALDFEVERQIG
VLESGGRIIQETRTWDEARGITVSMRSKEEAHDYRYFPDPDLVPLVLDNQWIDFIRESLPELPDQRRSRYITEYGLPEYD
AALLTLTKEMSDYFEEVMEHYNNPKAISNWIMGELSRLLNAGGIEITACKIKPAQLAEMLKLLDKGTISGKIAKAVFEEM
FASGKDPEAIVKEKGLVQISDEGAIASIVDEVIAANPKSVQDYQAGKTQAIGFLVGQVMKATKGKANPEMVNKMLREKL

Sequences:

>Translated_479_residues
MTQQYETVIGIEVHVELKTNTKIFCNSTTEFGGDPNHHTCPVCLGLPGTLPVLNKKVVEYAVRASLALNCQVANFSKFDR
KNYYYPDLPKNYQISQYDLPIAEHGYLDIVADGQPKRIGITRLHMEEDAGKLVHQGNIVTTPYSLVDYNRTGVPLIEIVS
EPDMRSSEEARAYVEKLKAIVQYTGVSDCRMEEGSLRCDVNVSVRPVGQKEFGTKAEIKNLNSFRALQRALDFEVERQIG
VLESGGRIIQETRTWDEARGITVSMRSKEEAHDYRYFPDPDLVPLVLDNQWIDFIRESLPELPDQRRSRYITEYGLPEYD
AALLTLTKEMSDYFEEVMEHYNNPKAISNWIMGELSRLLNAGGIEITACKIKPAQLAEMLKLLDKGTISGKIAKAVFEEM
FASGKDPEAIVKEKGLVQISDEGAIASIVDEVIAANPKSVQDYQAGKTQAIGFLVGQVMKATKGKANPEMVNKMLREKL
>Mature_478_residues
TQQYETVIGIEVHVELKTNTKIFCNSTTEFGGDPNHHTCPVCLGLPGTLPVLNKKVVEYAVRASLALNCQVANFSKFDRK
NYYYPDLPKNYQISQYDLPIAEHGYLDIVADGQPKRIGITRLHMEEDAGKLVHQGNIVTTPYSLVDYNRTGVPLIEIVSE
PDMRSSEEARAYVEKLKAIVQYTGVSDCRMEEGSLRCDVNVSVRPVGQKEFGTKAEIKNLNSFRALQRALDFEVERQIGV
LESGGRIIQETRTWDEARGITVSMRSKEEAHDYRYFPDPDLVPLVLDNQWIDFIRESLPELPDQRRSRYITEYGLPEYDA
ALLTLTKEMSDYFEEVMEHYNNPKAISNWIMGELSRLLNAGGIEITACKIKPAQLAEMLKLLDKGTISGKIAKAVFEEMF
ASGKDPEAIVKEKGLVQISDEGAIASIVDEVIAANPKSVQDYQAGKTQAIGFLVGQVMKATKGKANPEMVNKMLREKL

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=41, Blast_Score=343, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI115534094, Length=304, Percent_Identity=38.1578947368421, Blast_Score=214, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6319391, Length=521, Percent_Identity=31.8618042226488, Blast_Score=235, Evalue=1e-62,
Organism=Drosophila melanogaster, GI45550808, Length=498, Percent_Identity=33.5341365461847, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53768; Mature: 53637

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQYETVIGIEVHVELKTNTKIFCNSTTEFGGDPNHHTCPVCLGLPGTLPVLNKKVVEY
CCCCCCEEEEEEEEEEEECCCEEEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
AVRASLALNCQVANFSKFDRKNYYYPDLPKNYQISQYDLPIAEHGYLDIVADGQPKRIGI
HHHHHEEEEEEECCCHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEECCCCCEECE
TRLHMEEDAGKLVHQGNIVTTPYSLVDYNRTGVPLIEIVSEPDMRSSEEARAYVEKLKAI
EEEEEHHHHHHHEECCCEEECCHHHEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
VQYTGVSDCRMEEGSLRCDVNVSVRPVGQKEFGTKAEIKNLNSFRALQRALDFEVERQIG
HHHCCCCCCEECCCCEEEEEEEEEEECCCHHCCCHHHHHCCHHHHHHHHHHCHHHHHHCC
VLESGGRIIQETRTWDEARGITVSMRSKEEAHDYRYFPDPDLVPLVLDNQWIDFIRESLP
EECCCCCHHHHHHCHHHHCCEEEEECCCHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHC
ELPDQRRSRYITEYGLPEYDAALLTLTKEMSDYFEEVMEHYNNPKAISNWIMGELSRLLN
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
AGGIEITACKIKPAQLAEMLKLLDKGTISGKIAKAVFEEMFASGKDPEAIVKEKGLVQIS
CCCCEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEC
DEGAIASIVDEVIAANPKSVQDYQAGKTQAIGFLVGQVMKATKGKANPEMVNKMLREKL
CCCCHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TQQYETVIGIEVHVELKTNTKIFCNSTTEFGGDPNHHTCPVCLGLPGTLPVLNKKVVEY
CCCCCEEEEEEEEEEEECCCEEEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
AVRASLALNCQVANFSKFDRKNYYYPDLPKNYQISQYDLPIAEHGYLDIVADGQPKRIGI
HHHHHEEEEEEECCCHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEECCCCCEECE
TRLHMEEDAGKLVHQGNIVTTPYSLVDYNRTGVPLIEIVSEPDMRSSEEARAYVEKLKAI
EEEEEHHHHHHHEECCCEEECCHHHEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
VQYTGVSDCRMEEGSLRCDVNVSVRPVGQKEFGTKAEIKNLNSFRALQRALDFEVERQIG
HHHCCCCCCEECCCCEEEEEEEEEEECCCHHCCCHHHHHCCHHHHHHHHHHCHHHHHHCC
VLESGGRIIQETRTWDEARGITVSMRSKEEAHDYRYFPDPDLVPLVLDNQWIDFIRESLP
EECCCCCHHHHHHCHHHHCCEEEEECCCHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHC
ELPDQRRSRYITEYGLPEYDAALLTLTKEMSDYFEEVMEHYNNPKAISNWIMGELSRLLN
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
AGGIEITACKIKPAQLAEMLKLLDKGTISGKIAKAVFEEMFASGKDPEAIVKEKGLVQIS
CCCCEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEC
DEGAIASIVDEVIAANPKSVQDYQAGKTQAIGFLVGQVMKATKGKANPEMVNKMLREKL
CCCCHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA