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The map label for this gene is aksA [H]

Identifier: 134300178

GI number: 134300178

Start: 2556932

End: 2558065

Strand: Reverse

Name: aksA [H]

Synonym: Dred_2339

Alternate gene names: 134300178

Gene position: 2558065-2556932 (Counterclockwise)

Preceding gene: 134300179

Following gene: 134300177

Centisome position: 70.9

GC content: 44.53

Gene sequence:

>1134_bases
GTGGATAACAGAAAAATTTGGATTGTAGATACAACATTGCGTGACGGCGAGCAAACCGCCGGCGTGGTGTTTGCAAACAG
AGAAAAGGTGCGAATTGCACAGTTTCTCAATGAACTGGGTGTTGATCAAATTGAAGCAGGTATTCCTGTAATGGGTGGAG
ACGAGCAGGAAGCAATTAAAGAAATTTGCAAACTGGGTCTAAAGTCTAGCATTATGGGCTGGAATCGCCCGGTGCTTAAA
GATATTGAATCATCCATAGCCTGCGGAGTGGATGCTGTGGCCATATCCATTTCTACATCGGACATTCATATTAAGCATAA
GCTAAAAACCAGTCGTGAATGGGTTCTGGAGCATATGGTTCGGGCCACGGAATTTGCCAAAAAGGAAGGCATGTATATTT
CTGTTAACGCAGAGGATGCTTCCCGTAGTGATCTGAATTTTTTAATTGAGTTTGCCAAGGCCGCTAAGCAGGCTGGTGCA
GACCGGCTTCGCTACTGCGATACGGTTGGTATTTTGGAACCCTTTACCTGTTATGATAACATTAAAAAGCTAAAGGAAGC
AGTGGACATTGAGGTTGAAATGCACACCCATAATGACTTTGGCATGGCAACAGCCAATGCCTTTGCCGGGGTTAAGGCCG
GGGCCAATTGGGTAGGGGTAACCATTACAGGTTTGGGTGAACGAGCTGGTAACTCACCTTTGGAAGAAGTTGTTATGGCC
TTAAAGCATCTCTATGATATTGACTTGAATTTTAAAACTGAGATGTTTCGAGAAGTGGCAGAATATGTTGCCCGGGCCTC
TGGCCGGGAATTACACTGCAGTAAGGCCATTGTAGGTTCCAATATGTTTGCCCATGAGTCTGGTATTCATGCCGACGGGG
CCATTAAGAACCCTAAAACCTATGAAGCATTCCAACCGGAAGAAGTGGGCTTGGAACGGCAGATCGTGATTGGTAAACAT
TCCGGCAGTGCTTCCCTGAGAATGAAGTTTGCCGAGTATGGCATTGACTTATCCAAGGAAGAAGCCGAAGAATTATTGCC
TAAAATACGTTCTGCATCAGTATCCTTAAAGCGATCCTTGTTTGATAAAGAATTGGTTTATATCTATGAAGATCATTTCG
GAAAGAGGGATTAA

Upstream 100 bases:

>100_bases
ATAAATGTATACAGTATAAATAGCTAATACCCAATATTCATAATGACTCCATTGTGATAAGATAAATCTTAAGTTTTACT
TGGCATATGGAGGTGTTACT

Downstream 100 bases:

>100_bases
TTTGGGCAAAACTATTATTGAGAAGATATTATCTTCTCATAGCGGCCAGGATTGCCAGGCCAATGACATCGTGGTAGCCC
AGGTTGATTTTGTCATGGGA

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MDNRKIWIVDTTLRDGEQTAGVVFANREKVRIAQFLNELGVDQIEAGIPVMGGDEQEAIKEICKLGLKSSIMGWNRPVLK
DIESSIACGVDAVAISISTSDIHIKHKLKTSREWVLEHMVRATEFAKKEGMYISVNAEDASRSDLNFLIEFAKAAKQAGA
DRLRYCDTVGILEPFTCYDNIKKLKEAVDIEVEMHTHNDFGMATANAFAGVKAGANWVGVTITGLGERAGNSPLEEVVMA
LKHLYDIDLNFKTEMFREVAEYVARASGRELHCSKAIVGSNMFAHESGIHADGAIKNPKTYEAFQPEEVGLERQIVIGKH
SGSASLRMKFAEYGIDLSKEEAEELLPKIRSASVSLKRSLFDKELVYIYEDHFGKRD

Sequences:

>Translated_377_residues
MDNRKIWIVDTTLRDGEQTAGVVFANREKVRIAQFLNELGVDQIEAGIPVMGGDEQEAIKEICKLGLKSSIMGWNRPVLK
DIESSIACGVDAVAISISTSDIHIKHKLKTSREWVLEHMVRATEFAKKEGMYISVNAEDASRSDLNFLIEFAKAAKQAGA
DRLRYCDTVGILEPFTCYDNIKKLKEAVDIEVEMHTHNDFGMATANAFAGVKAGANWVGVTITGLGERAGNSPLEEVVMA
LKHLYDIDLNFKTEMFREVAEYVARASGRELHCSKAIVGSNMFAHESGIHADGAIKNPKTYEAFQPEEVGLERQIVIGKH
SGSASLRMKFAEYGIDLSKEEAEELLPKIRSASVSLKRSLFDKELVYIYEDHFGKRD
>Mature_377_residues
MDNRKIWIVDTTLRDGEQTAGVVFANREKVRIAQFLNELGVDQIEAGIPVMGGDEQEAIKEICKLGLKSSIMGWNRPVLK
DIESSIACGVDAVAISISTSDIHIKHKLKTSREWVLEHMVRATEFAKKEGMYISVNAEDASRSDLNFLIEFAKAAKQAGA
DRLRYCDTVGILEPFTCYDNIKKLKEAVDIEVEMHTHNDFGMATANAFAGVKAGANWVGVTITGLGERAGNSPLEEVVMA
LKHLYDIDLNFKTEMFREVAEYVARASGRELHCSKAIVGSNMFAHESGIHADGAIKNPKTYEAFQPEEVGLERQIVIGKH
SGSASLRMKFAEYGIDLSKEEAEELLPKIRSASVSLKRSLFDKELVYIYEDHFGKRD

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=383, Percent_Identity=34.7258485639687, Blast_Score=192, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6320019, Length=346, Percent_Identity=32.0809248554913, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6320071, Length=350, Percent_Identity=31.7142857142857, Blast_Score=196, Evalue=6e-51,
Organism=Drosophila melanogaster, GI24582381, Length=288, Percent_Identity=25.6944444444444, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 41892; Mature: 41892

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNRKIWIVDTTLRDGEQTAGVVFANREKVRIAQFLNELGVDQIEAGIPVMGGDEQEAIK
CCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHH
EICKLGLKSSIMGWNRPVLKDIESSIACGVDAVAISISTSDIHIKHKLKTSREWVLEHMV
HHHHHCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEECCCEEEEEHHHHHHHHHHHHHH
RATEFAKKEGMYISVNAEDASRSDLNFLIEFAKAAKQAGADRLRYCDTVGILEPFTCYDN
HHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHH
IKKLKEAVDIEVEMHTHNDFGMATANAFAGVKAGANWVGVTITGLGERAGNSPLEEVVMA
HHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHH
LKHLYDIDLNFKTEMFREVAEYVARASGRELHCSKAIVGSNMFAHESGIHADGAIKNPKT
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCC
YEAFQPEEVGLERQIVIGKHSGSASLRMKFAEYGIDLSKEEAEELLPKIRSASVSLKRSL
CCCCCCHHCCCCEEEEEEECCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
FDKELVYIYEDHFGKRD
CCCCEEEEEECCCCCCC
>Mature Secondary Structure
MDNRKIWIVDTTLRDGEQTAGVVFANREKVRIAQFLNELGVDQIEAGIPVMGGDEQEAIK
CCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHH
EICKLGLKSSIMGWNRPVLKDIESSIACGVDAVAISISTSDIHIKHKLKTSREWVLEHMV
HHHHHCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEECCCEEEEEHHHHHHHHHHHHHH
RATEFAKKEGMYISVNAEDASRSDLNFLIEFAKAAKQAGADRLRYCDTVGILEPFTCYDN
HHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHH
IKKLKEAVDIEVEMHTHNDFGMATANAFAGVKAGANWVGVTITGLGERAGNSPLEEVVMA
HHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHH
LKHLYDIDLNFKTEMFREVAEYVARASGRELHCSKAIVGSNMFAHESGIHADGAIKNPKT
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCC
YEAFQPEEVGLERQIVIGKHSGSASLRMKFAEYGIDLSKEEAEELLPKIRSASVSLKRSL
CCCCCCHHCCCCEEEEEEECCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
FDKELVYIYEDHFGKRD
CCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]