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The map label for this gene is alaXL [H]

Identifier: 134300172

GI number: 134300172

Start: 2549381

End: 2550595

Strand: Reverse

Name: alaXL [H]

Synonym: Dred_2333

Alternate gene names: 134300172

Gene position: 2550595-2549381 (Counterclockwise)

Preceding gene: 134300173

Following gene: 134300168

Centisome position: 70.69

GC content: 47.16

Gene sequence:

>1215_bases
TTGACAGGAATATACCAGGTCGATCCTTACCTGCTGCAATACCGTACCAACGTCAAAGGATTTATCGAAGATCAAGACAA
AACCGGCATTTTATTTGAGGAAACTATCTTTTACCCGGAGGGGGGAGGTCAACCATCCGATCGGGGAACCCTTGTCTGTG
ATGCCCGTCGAACATCCCATGAAGTATTGCATGTGGAACAAAGGCCCGAGGGCATTGTGCACTGGATTGCCGGGTCCCTG
AAGCCCACCATAGGTGCCAGTGTGTTATTGACTGTGGATGCAGAAAGACGGCAAGATCATATGCAACAACATCATGGTCA
ACACATACTGTCTGCTGTCTTTGAACGTAATTATGGTTGGGATACAGTGGGATTTCATTTAGGCCCGGAGACTTCCACCA
TTGACTTAACTGTGGGGGACATTTCACAGGATGTATTGCAAGAAGTGGAACTGGAAGTAAACCGGATTATCATGGAAAAT
ATTCCGGTGAAAACAGAATATTATAAGCGTGAAGATCTTGGGCAGGAATTAATTAAGAAACTACCGTCGGATCAAAATGA
AGTACGGTTGGTGGTGATCCCGGGTATTGATGAAAATGCTTGTTGTGGTACTCACCCCCAGCGTACAGGTGAGGTAGGTC
CCTGTAAAATTCTAAAAATAGAAAAGTTAAGGGGAAACATAAGGTTGTCCTTTATTTGTGGCGAACGAACCATTGCATGG
ATGAGACAAACCGCCGACACTCTTCACCAGTTAGAGAAGGCTATCGGAGCTTCTGGTAATGAAGCCCTTATTCGGTTAGA
AAAAAGAGAAGTGGAGTTGAAGAAACTCCAAAAGGAACGTAAGGAATTACTGTCCCTCAAATATCGTTATCTGGCTAAGG
AGTTGGCAACGGAGGCCATTTCCTCGGGTGAAACAAGTATTCTAATACAACACTTTTCTGAAGCTGACATGGAAATGCTG
CGGGGGGTGGCTACCTCCTGGTGTGCCCAGTCGGGCAGGGTGGCTGTACTAATGGGTGGTACCGGTCCCTTTGATGTGGT
AATGGCCAGGGGTTCAGGGGTAGCAACCTCTATGAACAGTGTGGCTGCCGAACTATGGCCAGTGCTGGAGGGTAAAGGCG
GCGGCAGTGCTGAGCTGGTCCAAGGCAAGGCTCAGAAATTACCAGTGGAACAATTAAAGGAAATGGTTGCCTCAGCCATC
AACCGTAAGCCTTAA

Upstream 100 bases:

>100_bases
GCTGATAACTTACAGAACAACAGACCACCCACAAGGAATTTTCTAAGCTATGTCGAAAGTTATGTTCATTAGTTCGTAAC
CCTGAGAGGAGAGGATAGTT

Downstream 100 bases:

>100_bases
GGGATAGTCTTTAGGGTTTAGCCATTAGCCATTTAATATTGTTAAACTAAAAAATAACATCTAATAGCTAAAGGCTGAGG
GCTAAATATAATATTGAATA

Product: phage tail protein I

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTGIYQVDPYLLQYRTNVKGFIEDQDKTGILFEETIFYPEGGGQPSDRGTLVCDARRTSHEVLHVEQRPEGIVHWIAGSL
KPTIGASVLLTVDAERRQDHMQQHHGQHILSAVFERNYGWDTVGFHLGPETSTIDLTVGDISQDVLQEVELEVNRIIMEN
IPVKTEYYKREDLGQELIKKLPSDQNEVRLVVIPGIDENACCGTHPQRTGEVGPCKILKIEKLRGNIRLSFICGERTIAW
MRQTADTLHQLEKAIGASGNEALIRLEKREVELKKLQKERKELLSLKYRYLAKELATEAISSGETSILIQHFSEADMEML
RGVATSWCAQSGRVAVLMGGTGPFDVVMARGSGVATSMNSVAAELWPVLEGKGGGSAELVQGKAQKLPVEQLKEMVASAI
NRKP

Sequences:

>Translated_404_residues
MTGIYQVDPYLLQYRTNVKGFIEDQDKTGILFEETIFYPEGGGQPSDRGTLVCDARRTSHEVLHVEQRPEGIVHWIAGSL
KPTIGASVLLTVDAERRQDHMQQHHGQHILSAVFERNYGWDTVGFHLGPETSTIDLTVGDISQDVLQEVELEVNRIIMEN
IPVKTEYYKREDLGQELIKKLPSDQNEVRLVVIPGIDENACCGTHPQRTGEVGPCKILKIEKLRGNIRLSFICGERTIAW
MRQTADTLHQLEKAIGASGNEALIRLEKREVELKKLQKERKELLSLKYRYLAKELATEAISSGETSILIQHFSEADMEML
RGVATSWCAQSGRVAVLMGGTGPFDVVMARGSGVATSMNSVAAELWPVLEGKGGGSAELVQGKAQKLPVEQLKEMVASAI
NRKP
>Mature_403_residues
TGIYQVDPYLLQYRTNVKGFIEDQDKTGILFEETIFYPEGGGQPSDRGTLVCDARRTSHEVLHVEQRPEGIVHWIAGSLK
PTIGASVLLTVDAERRQDHMQQHHGQHILSAVFERNYGWDTVGFHLGPETSTIDLTVGDISQDVLQEVELEVNRIIMENI
PVKTEYYKREDLGQELIKKLPSDQNEVRLVVIPGIDENACCGTHPQRTGEVGPCKILKIEKLRGNIRLSFICGERTIAWM
RQTADTLHQLEKAIGASGNEALIRLEKREVELKKLQKERKELLSLKYRYLAKELATEAISSGETSILIQHFSEADMEMLR
GVATSWCAQSGRVAVLMGGTGPFDVVMARGSGVATSMNSVAAELWPVLEGKGGGSAELVQGKAQKLPVEQLKEMVASAIN
RKP

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=365, Percent_Identity=31.7808219178082, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI217416402, Length=365, Percent_Identity=31.7808219178082, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI38569417, Length=422, Percent_Identity=25.3554502369668, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789048, Length=374, Percent_Identity=25.668449197861, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324288, Length=318, Percent_Identity=31.1320754716981, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI18543305, Length=406, Percent_Identity=29.3103448275862, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 44882; Mature: 44751

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGIYQVDPYLLQYRTNVKGFIEDQDKTGILFEETIFYPEGGGQPSDRGTLVCDARRTSH
CCCCEECCHHHEEEECCCCCEEECCCCCCEEEEEEEEECCCCCCCCCCCCEEECCCCCCC
EVLHVEQRPEGIVHWIAGSLKPTIGASVLLTVDAERRQDHMQQHHGQHILSAVFERNYGW
CEEEECCCCCHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCC
DTVGFHLGPETSTIDLTVGDISQDVLQEVELEVNRIIMENIPVKTEYYKREDLGQELIKK
CCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LPSDQNEVRLVVIPGIDENACCGTHPQRTGEVGPCKILKIEKLRGNIRLSFICGERTIAW
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCHHHHH
MRQTADTLHQLEKAIGASGNEALIRLEKREVELKKLQKERKELLSLKYRYLAKELATEAI
HHHHHHHHHHHHHHHCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSGETSILIQHFSEADMEMLRGVATSWCAQSGRVAVLMGGTGPFDVVMARGSGVATSMNS
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCHHHHHH
VAAELWPVLEGKGGGSAELVQGKAQKLPVEQLKEMVASAINRKP
HHHHHHHHHCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TGIYQVDPYLLQYRTNVKGFIEDQDKTGILFEETIFYPEGGGQPSDRGTLVCDARRTSH
CCCEECCHHHEEEECCCCCEEECCCCCCEEEEEEEEECCCCCCCCCCCCEEECCCCCCC
EVLHVEQRPEGIVHWIAGSLKPTIGASVLLTVDAERRQDHMQQHHGQHILSAVFERNYGW
CEEEECCCCCHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCC
DTVGFHLGPETSTIDLTVGDISQDVLQEVELEVNRIIMENIPVKTEYYKREDLGQELIKK
CCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LPSDQNEVRLVVIPGIDENACCGTHPQRTGEVGPCKILKIEKLRGNIRLSFICGERTIAW
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCHHHHH
MRQTADTLHQLEKAIGASGNEALIRLEKREVELKKLQKERKELLSLKYRYLAKELATEAI
HHHHHHHHHHHHHHHCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSGETSILIQHFSEADMEMLRGVATSWCAQSGRVAVLMGGTGPFDVVMARGSGVATSMNS
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCHHHHHH
VAAELWPVLEGKGGGSAELVQGKAQKLPVEQLKEMVASAINRKP
HHHHHHHHHCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]