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The map label for this gene is hisJ [H]

Identifier: 134300077

GI number: 134300077

Start: 2444036

End: 2444875

Strand: Reverse

Name: hisJ [H]

Synonym: Dred_2235

Alternate gene names: 134300077

Gene position: 2444875-2444036 (Counterclockwise)

Preceding gene: 134300078

Following gene: 134300076

Centisome position: 67.76

GC content: 37.02

Gene sequence:

>840_bases
ATGAAGAAAAAAATTTCAATATTAATCGCAATGTCTCTTGCAGTCATGTTGCTATTAACAGGATGTGGATCAGGTGGTAG
TTCAGAAACAAAAAAAGAAAAAACAGGTGGAACATTTAAGGTAGGTCTTGAGTGTGGATATGCACCATTCAACTGGACTC
AAATGGATGATTCCAATGGCGCAGTTAAAATTGATGGCAATGCTGAATATGCAGGTGGCTATGATGTAGAAATTGCTAAA
AAGATTGCCAAAGGTCTAGGAAAAGAATTAGTTATTGTAAAAACTGAATGGGATGGCCTTGTTCCAGCATTAACTTCAGG
TAAAATTGATGCAATTATCGCAGGTATGTCACCAACTGCAGAACGTAAAAAAACAATCGACTTCTCAGATATCTACTATA
AATCTGATTTAGTTATGGTTGTTAAAAAAGGTGGCAAGTATGAAGGAGCTAAATCTATCCAAGATTTCAATGGAGCAAAA
GTAACTGCTCAATTAAATACTTTCCACTATACAGTAATCGATCAAATTAAAGGTGTTGCCAAACAACCAGCTATGGATAA
CTTTCCAGCAATGAGAGTGGCTCTTGAATCAGGTATTATTGATGGATACGTCTCAGAACGTCCAGAAGGCGTTAGTGCTT
CAACGGCTAACCCAAACTTTGCCATGGTAGAATTTAAAGAAGGCTTTACTACATCAGATGACGATACTGCAATTGCTGTA
GGTATTGCAAAAGGTAGTGAATTAACTGAACAAATTAATAAGATCCTGGCAGGTATCTCTGAGGAAGAACGTACACAAAT
TATGGATAGTGCCATCAAGAATCAACCGGCAGCTAAATAA

Upstream 100 bases:

>100_bases
TAAATGCTAAACAATGGAATAAACCCCTATAAACATTGACATTAGCTTTTTAGTTTCAAGGTACATATTAAACTAAATTT
AATTAGGAGGAGAAGTGGTC

Downstream 100 bases:

>100_bases
CAATAATTTAAAACCGGGTGCATTCAAAATGCACCCGGTTTTATAACGTTATTAAAGGAGGAGAAAGATGACCTTTGAGT
GGATAGTAAAAATTATTTCA

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: HBP; p29 [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKKKISILIAMSLAVMLLLTGCGSGGSSETKKEKTGGTFKVGLECGYAPFNWTQMDDSNGAVKIDGNAEYAGGYDVEIAK
KIAKGLGKELVIVKTEWDGLVPALTSGKIDAIIAGMSPTAERKKTIDFSDIYYKSDLVMVVKKGGKYEGAKSIQDFNGAK
VTAQLNTFHYTVIDQIKGVAKQPAMDNFPAMRVALESGIIDGYVSERPEGVSASTANPNFAMVEFKEGFTTSDDDTAIAV
GIAKGSELTEQINKILAGISEEERTQIMDSAIKNQPAAK

Sequences:

>Translated_279_residues
MKKKISILIAMSLAVMLLLTGCGSGGSSETKKEKTGGTFKVGLECGYAPFNWTQMDDSNGAVKIDGNAEYAGGYDVEIAK
KIAKGLGKELVIVKTEWDGLVPALTSGKIDAIIAGMSPTAERKKTIDFSDIYYKSDLVMVVKKGGKYEGAKSIQDFNGAK
VTAQLNTFHYTVIDQIKGVAKQPAMDNFPAMRVALESGIIDGYVSERPEGVSASTANPNFAMVEFKEGFTTSDDDTAIAV
GIAKGSELTEQINKILAGISEEERTQIMDSAIKNQPAAK
>Mature_279_residues
MKKKISILIAMSLAVMLLLTGCGSGGSSETKKEKTGGTFKVGLECGYAPFNWTQMDDSNGAVKIDGNAEYAGGYDVEIAK
KIAKGLGKELVIVKTEWDGLVPALTSGKIDAIIAGMSPTAERKKTIDFSDIYYKSDLVMVVKKGGKYEGAKSIQDFNGAK
VTAQLNTFHYTVIDQIKGVAKQPAMDNFPAMRVALESGIIDGYVSERPEGVSASTANPNFAMVEFKEGFTTSDDDTAIAV
GIAKGSELTEQINKILAGISEEERTQIMDSAIKNQPAAK

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787088, Length=230, Percent_Identity=26.5217391304348, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=26, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787031, Length=230, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788649, Length=133, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788648, Length=122, Percent_Identity=28.6885245901639, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29758; Mature: 29758

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKISILIAMSLAVMLLLTGCGSGGSSETKKEKTGGTFKVGLECGYAPFNWTQMDDSNG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEEECCCCCCCEEECCCCC
AVKIDGNAEYAGGYDVEIAKKIAKGLGKELVIVKTEWDGLVPALTSGKIDAIIAGMSPTA
EEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCH
ERKKTIDFSDIYYKSDLVMVVKKGGKYEGAKSIQDFNGAKVTAQLNTFHYTVIDQIKGVA
HHHHCCCHHHEEEECCEEEEEECCCCCCCCCHHHCCCCCEEEEEECEEEEHHHHHHHHHH
KQPAMDNFPAMRVALESGIIDGYVSERPEGVSASTANPNFAMVEFKEGFTTSDDDTAIAV
CCCCCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEE
GIAKGSELTEQINKILAGISEEERTQIMDSAIKNQPAAK
EECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKKISILIAMSLAVMLLLTGCGSGGSSETKKEKTGGTFKVGLECGYAPFNWTQMDDSNG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEEECCCCCCCEEECCCCC
AVKIDGNAEYAGGYDVEIAKKIAKGLGKELVIVKTEWDGLVPALTSGKIDAIIAGMSPTA
EEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCH
ERKKTIDFSDIYYKSDLVMVVKKGGKYEGAKSIQDFNGAKVTAQLNTFHYTVIDQIKGVA
HHHHCCCHHHEEEECCEEEEEECCCCCCCCCHHHCCCCCEEEEEECEEEEHHHHHHHHHH
KQPAMDNFPAMRVALESGIIDGYVSERPEGVSASTANPNFAMVEFKEGFTTSDDDTAIAV
CCCCCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEE
GIAKGSELTEQINKILAGISEEERTQIMDSAIKNQPAAK
EECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9192026; 9395059; 10688204 [H]