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The map label for this gene is opuD [H]

Identifier: 134300058

GI number: 134300058

Start: 2423658

End: 2425229

Strand: Reverse

Name: opuD [H]

Synonym: Dred_2215

Alternate gene names: 134300058

Gene position: 2425229-2423658 (Counterclockwise)

Preceding gene: 134300059

Following gene: 134300057

Centisome position: 67.22

GC content: 43.38

Gene sequence:

>1572_bases
TTGATTGTTCTTGCCTTTGTTTTATGGGGGGCCTTTTTTCCAGAAAGCCTTGGTCAGCAAGCAAACAGTATATTAGGGTT
TATGACGGAGAAGTTTGGTTGGTTTTATCTTTTAGCTACCTTTGGTTTTTTAGTGTTTGCTATTTACCTGGCATTTAGCA
AATACGGAAGAATCCGCTTGGGGGATGATGATGACAGGCCGGAATATTCCAATATTACTTGGTTTGGCATGTTGTTTAGC
GCGGGAATGGGAATCGGGCTGGTGTTCTGGGGTGTTGCAGAACCAGTAAGCCACTACACAAATCCTCCCCAGGGTTTGCA
GGGTGGAACAGCGGAAGCTGCCCGGACGGGTATGCTTTATGCCTTTTTTCATTGGGGTTTTCAGCCCTGGGCCATCTACA
CAATCATTGCTTTATCATTGGCATACTTTAATTTCCGGAAGGGCTATAGAGGGTTAATCAGTTCCACCTTTTACCCATTG
CTGGGGGATCGTATCAATGGTCCCCTTGGCAAAACTATTGACATACTGGCCATTGTGGCCACGGCCTTTGGCGTTGCTAC
TTCCCTTGGCTTAGGAACGCTTCAAATAAATGGTGGACTTGCGCATCTTTTAGGTATTCCCAATAACACCTTAGTCCATA
TTGTCATTATCGTCGTTGTAACAGTCTTATACATGATTTCAGCCACCACAGGGTTGGATAAAGGGATCAAATTTCTTAGT
AATGCCAATATTGCAGTGGCTACGTCCCTGCTTCTATTTGTATTGTTTCTGGGGCCAACCTCCTTTATTTTTGATACCCT
GACAACCACCCTAGGAGGTTACTTGCAAAATATTATCCAAATGAGCCTCCGTTTAACTCCCTTTACTCAGAATAAGTGGA
TTAGTAATTGGACCCTATTTTATTGGGCTTGGTGGATCGCTTGGGCACCCTTTGTGGGCATGTTTATTGCCCGGGTTTCT
AAGGGGAGAACAATCAAAGAATTTATCTTAGGAGTTCTATTAGTGCCCAGTCTGTTTAGCTTTTTCTGGTTTTCTACCTT
TGGAGGAACAGCTTTAAACCTTGAAGTTTTTGGCCATAAGGCTATTGCGGCAGCTGTCCAAGAGGATATTACTTCGGCAT
TATTTATTACCTTGGAGAAACTGCCCTTGGGAACAATATTGGCAGCCACAGCAACCCTCTTAATTATAACTTTTTTTGTT
ACCTCTGCAGATTCTGCCACCTTTGTTTTGGGGATGCTTTCCTCCAAGGGGGATAACAACCCCACCAATAGGGTGAAGCT
TACCTGGGGAATACTTCAGTCTTCTATTGCCACTGTCCTATTATTGAGCGGCGGATTACAGAGTTTACAGACTGCCTCAA
TTGTTACAGCACTACCCTTTGCCCTCATAATGGTATTCATGTGTTTTTCTATTATGAAGGCCCTTGAAGATGATTATGAT
GATATGGAAAGGGAATATCGAAAGCGGACTGGAACCAGGATTGCCCTTAAACCTTTACCAGATGGGAAAAAAAGGGCAGT
AGAACAAAAAAGTAATAACAAGACAGTTGAAATCCCGCAATCCCGTAAGTGA

Upstream 100 bases:

>100_bases
AAGCATAACGCTAAACAGAATAGATATCGCCTCAAAATATAAAAATAACTTTGAGTATAAAGAATTACAGGGGGCGTTTA
CTATATTTCTAGTTTCTGTA

Downstream 100 bases:

>100_bases
TTGCGGGGTTTTTATTATTCTAAGAATGATGTCAAAAACCGCAAAGGCTGATTTGTACGGGTAACGGTTTGAAATTGTTA
CTGTGGTGGACGGCACAAAG

Product: choline/carnitine/betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MIVLAFVLWGAFFPESLGQQANSILGFMTEKFGWFYLLATFGFLVFAIYLAFSKYGRIRLGDDDDRPEYSNITWFGMLFS
AGMGIGLVFWGVAEPVSHYTNPPQGLQGGTAEAARTGMLYAFFHWGFQPWAIYTIIALSLAYFNFRKGYRGLISSTFYPL
LGDRINGPLGKTIDILAIVATAFGVATSLGLGTLQINGGLAHLLGIPNNTLVHIVIIVVVTVLYMISATTGLDKGIKFLS
NANIAVATSLLLFVLFLGPTSFIFDTLTTTLGGYLQNIIQMSLRLTPFTQNKWISNWTLFYWAWWIAWAPFVGMFIARVS
KGRTIKEFILGVLLVPSLFSFFWFSTFGGTALNLEVFGHKAIAAAVQEDITSALFITLEKLPLGTILAATATLLIITFFV
TSADSATFVLGMLSSKGDNNPTNRVKLTWGILQSSIATVLLLSGGLQSLQTASIVTALPFALIMVFMCFSIMKALEDDYD
DMEREYRKRTGTRIALKPLPDGKKRAVEQKSNNKTVEIPQSRK

Sequences:

>Translated_523_residues
MIVLAFVLWGAFFPESLGQQANSILGFMTEKFGWFYLLATFGFLVFAIYLAFSKYGRIRLGDDDDRPEYSNITWFGMLFS
AGMGIGLVFWGVAEPVSHYTNPPQGLQGGTAEAARTGMLYAFFHWGFQPWAIYTIIALSLAYFNFRKGYRGLISSTFYPL
LGDRINGPLGKTIDILAIVATAFGVATSLGLGTLQINGGLAHLLGIPNNTLVHIVIIVVVTVLYMISATTGLDKGIKFLS
NANIAVATSLLLFVLFLGPTSFIFDTLTTTLGGYLQNIIQMSLRLTPFTQNKWISNWTLFYWAWWIAWAPFVGMFIARVS
KGRTIKEFILGVLLVPSLFSFFWFSTFGGTALNLEVFGHKAIAAAVQEDITSALFITLEKLPLGTILAATATLLIITFFV
TSADSATFVLGMLSSKGDNNPTNRVKLTWGILQSSIATVLLLSGGLQSLQTASIVTALPFALIMVFMCFSIMKALEDDYD
DMEREYRKRTGTRIALKPLPDGKKRAVEQKSNNKTVEIPQSRK
>Mature_523_residues
MIVLAFVLWGAFFPESLGQQANSILGFMTEKFGWFYLLATFGFLVFAIYLAFSKYGRIRLGDDDDRPEYSNITWFGMLFS
AGMGIGLVFWGVAEPVSHYTNPPQGLQGGTAEAARTGMLYAFFHWGFQPWAIYTIIALSLAYFNFRKGYRGLISSTFYPL
LGDRINGPLGKTIDILAIVATAFGVATSLGLGTLQINGGLAHLLGIPNNTLVHIVIIVVVTVLYMISATTGLDKGIKFLS
NANIAVATSLLLFVLFLGPTSFIFDTLTTTLGGYLQNIIQMSLRLTPFTQNKWISNWTLFYWAWWIAWAPFVGMFIARVS
KGRTIKEFILGVLLVPSLFSFFWFSTFGGTALNLEVFGHKAIAAAVQEDITSALFITLEKLPLGTILAATATLLIITFFV
TSADSATFVLGMLSSKGDNNPTNRVKLTWGILQSSIATVLLLSGGLQSLQTASIVTALPFALIMVFMCFSIMKALEDDYD
DMEREYRKRTGTRIALKPLPDGKKRAVEQKSNNKTVEIPQSRK

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=503, Percent_Identity=37.5745526838966, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1788102, Length=468, Percent_Identity=31.6239316239316, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1786224, Length=466, Percent_Identity=29.3991416309013, Blast_Score=211, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 57635; Mature: 57635

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLAFVLWGAFFPESLGQQANSILGFMTEKFGWFYLLATFGFLVFAIYLAFSKYGRIRL
CEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GDDDDRPEYSNITWFGMLFSAGMGIGLVFWGVAEPVSHYTNPPQGLQGGTAEAARTGMLY
CCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AFFHWGFQPWAIYTIIALSLAYFNFRKGYRGLISSTFYPLLGDRINGPLGKTIDILAIVA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
TAFGVATSLGLGTLQINGGLAHLLGIPNNTLVHIVIIVVVTVLYMISATTGLDKGIKFLS
HHHHHHHHCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
NANIAVATSLLLFVLFLGPTSFIFDTLTTTLGGYLQNIIQMSLRLTPFTQNKWISNWTLF
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
YWAWWIAWAPFVGMFIARVSKGRTIKEFILGVLLVPSLFSFFWFSTFGGTALNLEVFGHK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHH
AIAAAVQEDITSALFITLEKLPLGTILAATATLLIITFFVTSADSATFVLGMLSSKGDNN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
PTNRVKLTWGILQSSIATVLLLSGGLQSLQTASIVTALPFALIMVFMCFSIMKALEDDYD
CCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DMEREYRKRTGTRIALKPLPDGKKRAVEQKSNNKTVEIPQSRK
HHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCC
>Mature Secondary Structure
MIVLAFVLWGAFFPESLGQQANSILGFMTEKFGWFYLLATFGFLVFAIYLAFSKYGRIRL
CEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GDDDDRPEYSNITWFGMLFSAGMGIGLVFWGVAEPVSHYTNPPQGLQGGTAEAARTGMLY
CCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AFFHWGFQPWAIYTIIALSLAYFNFRKGYRGLISSTFYPLLGDRINGPLGKTIDILAIVA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
TAFGVATSLGLGTLQINGGLAHLLGIPNNTLVHIVIIVVVTVLYMISATTGLDKGIKFLS
HHHHHHHHCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
NANIAVATSLLLFVLFLGPTSFIFDTLTTTLGGYLQNIIQMSLRLTPFTQNKWISNWTLF
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
YWAWWIAWAPFVGMFIARVSKGRTIKEFILGVLLVPSLFSFFWFSTFGGTALNLEVFGHK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHH
AIAAAVQEDITSALFITLEKLPLGTILAATATLLIITFFVTSADSATFVLGMLSSKGDNN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
PTNRVKLTWGILQSSIATVLLLSGGLQSLQTASIVTALPFALIMVFMCFSIMKALEDDYD
CCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DMEREYRKRTGTRIALKPLPDGKKRAVEQKSNNKTVEIPQSRK
HHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]