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The map label for this gene is mnmE [H]

Identifier: 134299998

GI number: 134299998

Start: 2357396

End: 2358625

Strand: Reverse

Name: mnmE [H]

Synonym: Dred_2152

Alternate gene names: 134299998

Gene position: 2358625-2357396 (Counterclockwise)

Preceding gene: 134299999

Following gene: 134299997

Centisome position: 65.37

GC content: 46.42

Gene sequence:

>1230_bases
ATGGAAAACACACCCCGGGGTAATAGACTACATATTGCGATTTTTGGTAGGCGCAATGCTGGTAAATCAAGTCTGATTAA
CGCCCTAACGAGCCAGGATATTGCAGTGGTTTCCAATATCCCAGGAACCACCACTGACCCGGTATACAAAGCAATGGAGA
TTTTACCAGTTGGACCGGTGGTGATCATTGATACGGCAGGGATTGACGATAGTGGTGATTTGGGAGAACTTAGGGTAAAA
CGGTCAATGGATGTGTTAAATAAAGCAGACATGGTTTTACTGGTTATTGACGCTGGAAGCGGGGTTACCGATTATGAGTT
AAAGATTGCAAGACGCTGTCAGGAGAAAAAACTCCCGGTGGTTGTGGTCTTTAATAAGATTGATGCACACCATGTTACAG
AGCAACAAATCGAGGAGGCAAAAACGCAGTTGGCAGGCCCGACAGTGGTGGTTAGTGCCCTTAATGGTCAGGGTATTGGC
GATTTGAAAATTGCCATTGTTAATACTGCTCCGGCCACTTGGGATGACCAAGCCATTGTGGGGGACCTATTAAATCCCGG
CGACACTGCGGTTTTGGTGGTACCCATTGACTCAGCTGCGCCGAAGGGCCGTTTGATTTTACCCCAGGTACAAACCATCA
GGGATATTTTAGACCATGATGCAATGGCCTATGTAGCCAAAGAGAGCGAGTTAAAGGAATGTATAGCTAACTTTATAAAA
AAGCCGAAAATGGTTATTACAGACTCCCAAGCCTTTAGGGAAGTTGAAAAAGATACCCCAGAGGACATTCTGCTTACTTC
TTTCTCTATTCTTTTTGCCCGCTATAAGGGGGATCTAGAAACTCTGGTGGCAGGAGCCAAAGCCATAGAACGTTTAAAAC
CTGGCGATAAGGTACTGATTTCCGAGGCCTGTACCCATCACCGGATGGAAGATGATATCGGTACCGTGAAAATCCCTCGC
TGGTTACGTCAGGTTGTAGGAGGAGATTTAGAATTCCACTGGAGCAGTGGCCACCGTTTCCCAGAGGATTTATCGGATTA
TAAACTGGTGGTACATTGCGGGGCCTGTATGGTCAACCGTCGAGAAATGCTTTCCCGTATTATGCAGGTCCAGCAGGTAG
GGGTACCCATTGTTAATTACGGTGTCTGTATTGCCTTTGTAATGGGCATCCTGCGACGGGCCCTTTCACCCTTTCCGACC
ATACAACAAATGCTTAACGACAAAAAGTAA

Upstream 100 bases:

>100_bases
AAATCAGCCCTTAGTACAAAATACTAGGATAGGGGGATGCCAGTTAAAAGATAGTCCAACCAATTTAAATACCAGAGGTA
CGCAGAAGGAGGCATCAACA

Downstream 100 bases:

>100_bases
TGGAAAGGGGGCATAGCCTTTGCAAGATAATTTTTTTAAAGCTCTAACCAAAGCCGCCGCAGGAAACGAACTGACACGAC
AGGATGTTATCACCCTATTA

Product: small GTP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MENTPRGNRLHIAIFGRRNAGKSSLINALTSQDIAVVSNIPGTTTDPVYKAMEILPVGPVVIIDTAGIDDSGDLGELRVK
RSMDVLNKADMVLLVIDAGSGVTDYELKIARRCQEKKLPVVVVFNKIDAHHVTEQQIEEAKTQLAGPTVVVSALNGQGIG
DLKIAIVNTAPATWDDQAIVGDLLNPGDTAVLVVPIDSAAPKGRLILPQVQTIRDILDHDAMAYVAKESELKECIANFIK
KPKMVITDSQAFREVEKDTPEDILLTSFSILFARYKGDLETLVAGAKAIERLKPGDKVLISEACTHHRMEDDIGTVKIPR
WLRQVVGGDLEFHWSSGHRFPEDLSDYKLVVHCGACMVNRREMLSRIMQVQQVGVPIVNYGVCIAFVMGILRRALSPFPT
IQQMLNDKK

Sequences:

>Translated_409_residues
MENTPRGNRLHIAIFGRRNAGKSSLINALTSQDIAVVSNIPGTTTDPVYKAMEILPVGPVVIIDTAGIDDSGDLGELRVK
RSMDVLNKADMVLLVIDAGSGVTDYELKIARRCQEKKLPVVVVFNKIDAHHVTEQQIEEAKTQLAGPTVVVSALNGQGIG
DLKIAIVNTAPATWDDQAIVGDLLNPGDTAVLVVPIDSAAPKGRLILPQVQTIRDILDHDAMAYVAKESELKECIANFIK
KPKMVITDSQAFREVEKDTPEDILLTSFSILFARYKGDLETLVAGAKAIERLKPGDKVLISEACTHHRMEDDIGTVKIPR
WLRQVVGGDLEFHWSSGHRFPEDLSDYKLVVHCGACMVNRREMLSRIMQVQQVGVPIVNYGVCIAFVMGILRRALSPFPT
IQQMLNDKK
>Mature_409_residues
MENTPRGNRLHIAIFGRRNAGKSSLINALTSQDIAVVSNIPGTTTDPVYKAMEILPVGPVVIIDTAGIDDSGDLGELRVK
RSMDVLNKADMVLLVIDAGSGVTDYELKIARRCQEKKLPVVVVFNKIDAHHVTEQQIEEAKTQLAGPTVVVSALNGQGIG
DLKIAIVNTAPATWDDQAIVGDLLNPGDTAVLVVPIDSAAPKGRLILPQVQTIRDILDHDAMAYVAKESELKECIANFIK
KPKMVITDSQAFREVEKDTPEDILLTSFSILFARYKGDLETLVAGAKAIERLKPGDKVLISEACTHHRMEDDIGTVKIPR
WLRQVVGGDLEFHWSSGHRFPEDLSDYKLVVHCGACMVNRREMLSRIMQVQQVGVPIVNYGVCIAFVMGILRRALSPFPT
IQQMLNDKK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082120, Length=168, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI2367268, Length=157, Percent_Identity=33.7579617834395, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 44884; Mature: 44884

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTPRGNRLHIAIFGRRNAGKSSLINALTSQDIAVVSNIPGTTTDPVYKAMEILPVGPV
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCE
VIIDTAGIDDSGDLGELRVKRSMDVLNKADMVLLVIDAGSGVTDYELKIARRCQEKKLPV
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCE
VVVFNKIDAHHVTEQQIEEAKTQLAGPTVVVSALNGQGIGDLKIAIVNTAPATWDDQAIV
EEEEECCCCHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHH
GDLLNPGDTAVLVVPIDSAAPKGRLILPQVQTIRDILDHDAMAYVAKESELKECIANFIK
HHCCCCCCCEEEEEEECCCCCCCCEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
KPKMVITDSQAFREVEKDTPEDILLTSFSILFARYKGDLETLVAGAKAIERLKPGDKVLI
CCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHH
SEACTHHRMEDDIGTVKIPRWLRQVVGGDLEFHWSSGHRFPEDLSDYKLVVHCGACMVNR
HHHHHHHHHHCCCCCEEHHHHHHHHHCCCEEEEECCCCCCCCCHHHCEEEEEHHHHHHHH
REMLSRIMQVQQVGVPIVNYGVCIAFVMGILRRALSPFPTIQQMLNDKK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC
>Mature Secondary Structure
MENTPRGNRLHIAIFGRRNAGKSSLINALTSQDIAVVSNIPGTTTDPVYKAMEILPVGPV
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCE
VIIDTAGIDDSGDLGELRVKRSMDVLNKADMVLLVIDAGSGVTDYELKIARRCQEKKLPV
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCE
VVVFNKIDAHHVTEQQIEEAKTQLAGPTVVVSALNGQGIGDLKIAIVNTAPATWDDQAIV
EEEEECCCCHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHH
GDLLNPGDTAVLVVPIDSAAPKGRLILPQVQTIRDILDHDAMAYVAKESELKECIANFIK
HHCCCCCCCEEEEEEECCCCCCCCEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
KPKMVITDSQAFREVEKDTPEDILLTSFSILFARYKGDLETLVAGAKAIERLKPGDKVLI
CCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHH
SEACTHHRMEDDIGTVKIPRWLRQVVGGDLEFHWSSGHRFPEDLSDYKLVVHCGACMVNR
HHHHHHHHHHCCCCCEEHHHHHHHHHCCCEEEEECCCCCCCCCHHHCEEEEEHHHHHHHH
REMLSRIMQVQQVGVPIVNYGVCIAFVMGILRRALSPFPTIQQMLNDKK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]