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The map label for this gene is bioB [H]

Identifier: 134299997

GI number: 134299997

Start: 2356312

End: 2357376

Strand: Reverse

Name: bioB [H]

Synonym: Dred_2151

Alternate gene names: 134299997

Gene position: 2357376-2356312 (Counterclockwise)

Preceding gene: 134299998

Following gene: 134299996

Centisome position: 65.34

GC content: 44.23

Gene sequence:

>1065_bases
TTGCAAGATAATTTTTTTAAAGCTCTAACCAAAGCCGCCGCAGGAAACGAACTGACACGACAGGATGTTATCACCCTATT
AGGTGCTTTGCCAGGGGATGAAGAAACCAAATTGTTTGACATGGCTGACCAAGTAAGAAAGAAACATTTTGGAGACGAGG
TACACCTAAGAGGAATTATCGAATTCTCTAACTACTGCCGGAATGATTGCCACTACTGTGGGCTAAGGCGTAGTAATAAA
GATATTGAAAGATATCGTATACCCATAGAGGAAATTGTTGAAACAGCTAAATACGCCGCTCAATTGGGATACGGTACCAT
TGTCTTGCAGTCAGGAGAAGACTCCCGTTACAGTGGGGAAGACTTGGCGGAGATAATACGCCAGGTTAAGAAAGTAGGTA
ATTTTGCTGTTACAATCTGCGTAGGGGAACGCAGCCGTGAAGATTATGAATTAATGAAAAAGGCAGGGGCAGATCGCTAC
CTATTAAAACATGAAACTGCAGATTCTGGTTTGTTTACCCAGCTCAGACCCGGTACTACACTCAAAGAAAGAATTAAAGG
GTTAACTTGGCTGAAGGAATTAGGCTATCAAGTGGGTTCTGGAAATATCGTTGGGTTACCTGGCCAAACAATGGAAACCC
TGGCAGATGATATTCTCTTACTAAGGGCCTTAGATGTTGAAATGGCCGGCATTGGGCCATTTATACCACACCAACAGACT
CCCTTACGGGACAGCAAAACCGGTGATCTGGCACTGACCCTGAAAACCCTTGCTGTGGCACGACTGATACTGCCCCAAAC
ACATTTGCCTGCCACCACAGCGGTGGGCACCTTACATCCGGATGGTCGAAAGATGGCCCTACGTTGTGGCGCCAATGTTA
TTATGCCTAATTTATCCCCTGCCAGTTATCGGGAAAAGTATCAAATTTATCCTGAAAAAGCCGGCAGCAAAGAGAATGCC
GATGAGTCCCATGAAAAAATTACTTCATTGGTAAAAGAAATGGGAAGACGTATAAGTACAGGACAGGGTCATAGCCCAAA
AGATAAATTTAAAGACGAAAGGTAG

Upstream 100 bases:

>100_bases
ATTGCCTTTGTAATGGGCATCCTGCGACGGGCCCTTTCACCCTTTCCGACCATACAACAAATGCTTAACGACAAAAAGTA
ATGGAAAGGGGGCATAGCCT

Downstream 100 bases:

>100_bases
GAGATAGGCGTGGCAATAAATAAGGCTGAAATTTATAATAACCAATGGGAACCGGCAGATTTTATTAATGAGGAAGAAAT
CAACCGTTTATTGGAAGAAG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MQDNFFKALTKAAAGNELTRQDVITLLGALPGDEETKLFDMADQVRKKHFGDEVHLRGIIEFSNYCRNDCHYCGLRRSNK
DIERYRIPIEEIVETAKYAAQLGYGTIVLQSGEDSRYSGEDLAEIIRQVKKVGNFAVTICVGERSREDYELMKKAGADRY
LLKHETADSGLFTQLRPGTTLKERIKGLTWLKELGYQVGSGNIVGLPGQTMETLADDILLLRALDVEMAGIGPFIPHQQT
PLRDSKTGDLALTLKTLAVARLILPQTHLPATTAVGTLHPDGRKMALRCGANVIMPNLSPASYREKYQIYPEKAGSKENA
DESHEKITSLVKEMGRRISTGQGHSPKDKFKDER

Sequences:

>Translated_354_residues
MQDNFFKALTKAAAGNELTRQDVITLLGALPGDEETKLFDMADQVRKKHFGDEVHLRGIIEFSNYCRNDCHYCGLRRSNK
DIERYRIPIEEIVETAKYAAQLGYGTIVLQSGEDSRYSGEDLAEIIRQVKKVGNFAVTICVGERSREDYELMKKAGADRY
LLKHETADSGLFTQLRPGTTLKERIKGLTWLKELGYQVGSGNIVGLPGQTMETLADDILLLRALDVEMAGIGPFIPHQQT
PLRDSKTGDLALTLKTLAVARLILPQTHLPATTAVGTLHPDGRKMALRCGANVIMPNLSPASYREKYQIYPEKAGSKENA
DESHEKITSLVKEMGRRISTGQGHSPKDKFKDER
>Mature_354_residues
MQDNFFKALTKAAAGNELTRQDVITLLGALPGDEETKLFDMADQVRKKHFGDEVHLRGIIEFSNYCRNDCHYCGLRRSNK
DIERYRIPIEEIVETAKYAAQLGYGTIVLQSGEDSRYSGEDLAEIIRQVKKVGNFAVTICVGERSREDYELMKKAGADRY
LLKHETADSGLFTQLRPGTTLKERIKGLTWLKELGYQVGSGNIVGLPGQTMETLADDILLLRALDVEMAGIGPFIPHQQT
PLRDSKTGDLALTLKTLAVARLILPQTHLPATTAVGTLHPDGRKMALRCGANVIMPNLSPASYREKYQIYPEKAGSKENA
DESHEKITSLVKEMGRRISTGQGHSPKDKFKDER

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=272, Percent_Identity=25.3676470588235, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 39451; Mature: 39451

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDNFFKALTKAAAGNELTRQDVITLLGALPGDEETKLFDMADQVRKKHFGDEVHLRGII
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
EFSNYCRNDCHYCGLRRSNKDIERYRIPIEEIVETAKYAAQLGYGTIVLQSGEDSRYSGE
HHHHHHCCCHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHH
DLAEIIRQVKKVGNFAVTICVGERSREDYELMKKAGADRYLLKHETADSGLFTQLRPGTT
HHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCH
LKERIKGLTWLKELGYQVGSGNIVGLPGQTMETLADDILLLRALDVEMAGIGPFIPHQQT
HHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PLRDSKTGDLALTLKTLAVARLILPQTHLPATTAVGTLHPDGRKMALRCGANVIMPNLSP
CCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHCCCEEECCCCC
ASYREKYQIYPEKAGSKENADESHEKITSLVKEMGRRISTGQGHSPKDKFKDER
HHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCC
>Mature Secondary Structure
MQDNFFKALTKAAAGNELTRQDVITLLGALPGDEETKLFDMADQVRKKHFGDEVHLRGII
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
EFSNYCRNDCHYCGLRRSNKDIERYRIPIEEIVETAKYAAQLGYGTIVLQSGEDSRYSGE
HHHHHHCCCHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHH
DLAEIIRQVKKVGNFAVTICVGERSREDYELMKKAGADRYLLKHETADSGLFTQLRPGTT
HHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCH
LKERIKGLTWLKELGYQVGSGNIVGLPGQTMETLADDILLLRALDVEMAGIGPFIPHQQT
HHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PLRDSKTGDLALTLKTLAVARLILPQTHLPATTAVGTLHPDGRKMALRCGANVIMPNLSP
CCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHCCCEEECCCCC
ASYREKYQIYPEKAGSKENADESHEKITSLVKEMGRRISTGQGHSPKDKFKDER
HHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]