Click here to switch to the map view.

The map label for this gene is thiH [H]

Identifier: 134299996

GI number: 134299996

Start: 2354833

End: 2356302

Strand: Reverse

Name: thiH [H]

Synonym: Dred_2150

Alternate gene names: 134299996

Gene position: 2356302-2354833 (Counterclockwise)

Preceding gene: 134299997

Following gene: 134299995

Centisome position: 65.31

GC content: 42.86

Gene sequence:

>1470_bases
GTGGCAATAAATAAGGCTGAAATTTATAATAACCAATGGGAACCGGCAGATTTTATTAATGAGGAAGAAATCAACCGTTT
ATTGGAAGAAGGCAAAAAGACTTCTCCTGCTAGAGTGCGGGAAATCATTGAGAAGGCCAGAGAGGCCCGGGGGCTAACAC
CAGAAGAAGTGGCTATTTTACTGCAAAATGATGACCAAGATTTAATTAATTTCATGTTTCAGGTTGCTAGCGAAATAAAG
CTTAAAATCTATGGTAAGCGCTTGGTCTTGTTTGCTCCTTTATATGTTAGCGACCACTGTGTAAATAACTGCGTTTATTG
TGGTTACCGTTGTGATAACAAATTTAATCGTCGTAAGCTGACCCAGGCAGAGGTGGAAGAGGAAATCAAGATTTTAGAAT
CCCTAGGTCACAAGAGATTGGCAGTGGAGGCCGGGGAACATCCAGGTGAATGTCCCATCGAGTATGTTTTGGAGTGTTTA
AAAACCATCTACAGCATTAAGTTTGACAACGGTAGTATTCGTCGTTGCAACGTAAATATTGCGGCCACCACCATTGAGGA
ATACAAAATGTTAAAGGATGCTGGTATTGGAACCTATATTTTGTTCCAGGAAACCTATCATAGGGAAACCTATAAGAAAA
TGCATCCCAGTGGGCCCAAAGCAGATTACGACTGGCATACCACTGCCCATGACCGGGCTATGATGGCTGGTATTGATGAT
GTAGGGCTTGGTCCTCTGTTTGGTCTTTATGATTATAAATATGAAGTGATGGGTCTTATGTTCCACGCCCTTCATATGGA
AGAACGTTTTGGAGTAGGGCCTCACACCATTTCAGTACCCCGTATTCGCCCTGCCTTGGGAGTAAATCGAGAAAACTTGC
CCTATTTAGTAAATGATGAGCAATTTATGAAACTGGTTACCATTATTCGTCTGGCAGTTCCCTATACCGGCATGATTATT
TCTACCCGAGAAAGTCCTGAATACAGGGATCTGTTGTTAAATCGAGGAATATCCCAAATTTCCGCCGGTTCCTGCACCGG
GGTAGGAGGTTATAAGAAAGAATTAGAGCGTAAGGCACGCCTTGCCAGCGGTAGTAACAATTGTGGCTGTGGTGAAGAGG
ATAGCCCTCAATTCATGGTTGATGATCACCGCAGTCCTGATGAAGTTCTGCGCAATATTTGTCAATCCGGGTGGTTACCC
AGTTATTGTACGGCCTGCTATCGCAAAGGTCGTACTGGTGACCGCTTTATGACTCTGGCTAAGAGTGGAGAAATTCAAAA
TGTTTGCCAGCCCAATGCTATTTTGACCTTTAAGGAATACTTATTGGACTATGCATCCCCCGAGACAAAGGCAGTGGGAG
AAGAAACCATCCGTCAGCACCTGGGAGAAATTAAAAATGAGCAAGTTCGTAAGATAACAGAAGAAAGATTAAAACAAATT
GAGGCAGGAGAAAGGGATCTTTATTTTTAA

Upstream 100 bases:

>100_bases
AAAAATTACTTCATTGGTAAAAGAAATGGGAAGACGTATAAGTACAGGACAGGGTCATAGCCCAAAAGATAAATTTAAAG
ACGAAAGGTAGGAGATAGGC

Downstream 100 bases:

>100_bases
TAGAAAAGCCGCAGTGCTACTGCGGCTTTTCTATTTATTGGAAATCAGAAGTATAAAACTCTCCGACTTGCATAAGGGCA
ACTAAGGCTTACGCCGTCGC

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MAINKAEIYNNQWEPADFINEEEINRLLEEGKKTSPARVREIIEKAREARGLTPEEVAILLQNDDQDLINFMFQVASEIK
LKIYGKRLVLFAPLYVSDHCVNNCVYCGYRCDNKFNRRKLTQAEVEEEIKILESLGHKRLAVEAGEHPGECPIEYVLECL
KTIYSIKFDNGSIRRCNVNIAATTIEEYKMLKDAGIGTYILFQETYHRETYKKMHPSGPKADYDWHTTAHDRAMMAGIDD
VGLGPLFGLYDYKYEVMGLMFHALHMEERFGVGPHTISVPRIRPALGVNRENLPYLVNDEQFMKLVTIIRLAVPYTGMII
STRESPEYRDLLLNRGISQISAGSCTGVGGYKKELERKARLASGSNNCGCGEEDSPQFMVDDHRSPDEVLRNICQSGWLP
SYCTACYRKGRTGDRFMTLAKSGEIQNVCQPNAILTFKEYLLDYASPETKAVGEETIRQHLGEIKNEQVRKITEERLKQI
EAGERDLYF

Sequences:

>Translated_489_residues
MAINKAEIYNNQWEPADFINEEEINRLLEEGKKTSPARVREIIEKAREARGLTPEEVAILLQNDDQDLINFMFQVASEIK
LKIYGKRLVLFAPLYVSDHCVNNCVYCGYRCDNKFNRRKLTQAEVEEEIKILESLGHKRLAVEAGEHPGECPIEYVLECL
KTIYSIKFDNGSIRRCNVNIAATTIEEYKMLKDAGIGTYILFQETYHRETYKKMHPSGPKADYDWHTTAHDRAMMAGIDD
VGLGPLFGLYDYKYEVMGLMFHALHMEERFGVGPHTISVPRIRPALGVNRENLPYLVNDEQFMKLVTIIRLAVPYTGMII
STRESPEYRDLLLNRGISQISAGSCTGVGGYKKELERKARLASGSNNCGCGEEDSPQFMVDDHRSPDEVLRNICQSGWLP
SYCTACYRKGRTGDRFMTLAKSGEIQNVCQPNAILTFKEYLLDYASPETKAVGEETIRQHLGEIKNEQVRKITEERLKQI
EAGERDLYF
>Mature_488_residues
AINKAEIYNNQWEPADFINEEEINRLLEEGKKTSPARVREIIEKAREARGLTPEEVAILLQNDDQDLINFMFQVASEIKL
KIYGKRLVLFAPLYVSDHCVNNCVYCGYRCDNKFNRRKLTQAEVEEEIKILESLGHKRLAVEAGEHPGECPIEYVLECLK
TIYSIKFDNGSIRRCNVNIAATTIEEYKMLKDAGIGTYILFQETYHRETYKKMHPSGPKADYDWHTTAHDRAMMAGIDDV
GLGPLFGLYDYKYEVMGLMFHALHMEERFGVGPHTISVPRIRPALGVNRENLPYLVNDEQFMKLVTIIRLAVPYTGMIIS
TRESPEYRDLLLNRGISQISAGSCTGVGGYKKELERKARLASGSNNCGCGEEDSPQFMVDDHRSPDEVLRNICQSGWLPS
YCTACYRKGRTGDRFMTLAKSGEIQNVCQPNAILTFKEYLLDYASPETKAVGEETIRQHLGEIKNEQVRKITEERLKQIE
AGERDLYF

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=415, Percent_Identity=28.433734939759, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 55860; Mature: 55729

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINKAEIYNNQWEPADFINEEEINRLLEEGKKTSPARVREIIEKAREARGLTPEEVAIL
CCCCHHHHHCCCCCCHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHEEEE
LQNDDQDLINFMFQVASEIKLKIYGKRLVLFAPLYVSDHCVNNCVYCGYRCDNKFNRRKL
EECCCHHHHHHHHHHHHHHEEEEECCEEEEEECHHHHHHHHCCCEEEEEECCCCHHHHHH
TQAEVEEEIKILESLGHKRLAVEAGEHPGECPIEYVLECLKTIYSIKFDNGSIRRCNVNI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHEEECCCCCEEEEEEEE
AATTIEEYKMLKDAGIGTYILFQETYHRETYKKMHPSGPKADYDWHTTAHDRAMMAGIDD
EEHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCC
VGLGPLFGLYDYKYEVMGLMFHALHMEERFGVGPHTISVPRIRPALGVNRENLPYLVNDE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHCCCCCCCCEEECHH
QFMKLVTIIRLAVPYTGMIISTRESPEYRDLLLNRGISQISAGSCTGVGGYKKELERKAR
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHH
LASGSNNCGCGEEDSPQFMVDDHRSPDEVLRNICQSGWLPSYCTACYRKGRTGDRFMTLA
HHCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHEEEC
KSGEIQNVCQPNAILTFKEYLLDYASPETKAVGEETIRQHLGEIKNEQVRKITEERLKQI
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGERDLYF
CCCCCCCCC
>Mature Secondary Structure 
AINKAEIYNNQWEPADFINEEEINRLLEEGKKTSPARVREIIEKAREARGLTPEEVAIL
CCCHHHHHCCCCCCHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHEEEE
LQNDDQDLINFMFQVASEIKLKIYGKRLVLFAPLYVSDHCVNNCVYCGYRCDNKFNRRKL
EECCCHHHHHHHHHHHHHHEEEEECCEEEEEECHHHHHHHHCCCEEEEEECCCCHHHHHH
TQAEVEEEIKILESLGHKRLAVEAGEHPGECPIEYVLECLKTIYSIKFDNGSIRRCNVNI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHEEECCCCCEEEEEEEE
AATTIEEYKMLKDAGIGTYILFQETYHRETYKKMHPSGPKADYDWHTTAHDRAMMAGIDD
EEHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCC
VGLGPLFGLYDYKYEVMGLMFHALHMEERFGVGPHTISVPRIRPALGVNRENLPYLVNDE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHCCCCCCCCEEECHH
QFMKLVTIIRLAVPYTGMIISTRESPEYRDLLLNRGISQISAGSCTGVGGYKKELERKAR
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHH
LASGSNNCGCGEEDSPQFMVDDHRSPDEVLRNICQSGWLPSYCTACYRKGRTGDRFMTLA
HHCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHEEEC
KSGEIQNVCQPNAILTFKEYLLDYASPETKAVGEETIRQHLGEIKNEQVRKITEERLKQI
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGERDLYF
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]