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The map label for this gene is ribH

Identifier: 134299936

GI number: 134299936

Start: 2286172

End: 2286636

Strand: Reverse

Name: ribH

Synonym: Dred_2090

Alternate gene names: 134299936

Gene position: 2286636-2286172 (Counterclockwise)

Preceding gene: 134299937

Following gene: 134299934

Centisome position: 63.38

GC content: 44.3

Gene sequence:

>465_bases
ATGCCGAAAGTATTTGAAGGACATTTACTGGGACAAGAATTAAAGTTTGGTATTGTTGTTGGTCGTTTTAACGAATTTAT
TACTAATAAATTGTTAAGCGGAGCACTAGATGCCTTGAAACGTCATGGCGTAGAGGACGAAAATGTTGAAATTGCTTATG
TTCCCGGGGCCTATGAAATTCCTTTGGTGGCAAAGAAAATGGCTGATAGCAAACGTTACGATGGCGTTATTTGCTTGGGG
GCGGTAATTCGTGGCGCAACCCCTCACTTTGAATACGTATCAGCGGAAGTTTCCAAAGGGGTGGCAAAGATCAGTTTGGA
TAGCAATCTGCCGGTGATCTTTGGCGTACTGACCGTAGATACCATCGAACAGGCCATCGAGCGGGCTGGTACCAAGGCTG
GCAACAAAGGTTGGGAAGCTGCCAATGCTGCCATTGAAATGGCCAATTTATTAAAAACTATCTAG

Upstream 100 bases:

>100_bases
AAATGCAAACTGGGTCATCTTTTAACCCTGGAAGAAAAGTCTAAGAAATCGGTCAATGAATAAAATCATTACGAATTATA
AGTAACAGGAGGACAAAAAT

Downstream 100 bases:

>100_bases
GAAAAACACTAAGAAAACCCGGAATTTTTCCGGGTTTTCTTAGTTTATCAAAAGTATTTAAGTTTTCGATACAATTGATG
CCTGGGTCAGGGCAAATAAA

Product: riboflavin synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MPKVFEGHLLGQELKFGIVVGRFNEFITNKLLSGALDALKRHGVEDENVEIAYVPGAYEIPLVAKKMADSKRYDGVICLG
AVIRGATPHFEYVSAEVSKGVAKISLDSNLPVIFGVLTVDTIEQAIERAGTKAGNKGWEAANAAIEMANLLKTI

Sequences:

>Translated_154_residues
MPKVFEGHLLGQELKFGIVVGRFNEFITNKLLSGALDALKRHGVEDENVEIAYVPGAYEIPLVAKKMADSKRYDGVICLG
AVIRGATPHFEYVSAEVSKGVAKISLDSNLPVIFGVLTVDTIEQAIERAGTKAGNKGWEAANAAIEMANLLKTI
>Mature_153_residues
PKVFEGHLLGQELKFGIVVGRFNEFITNKLLSGALDALKRHGVEDENVEIAYVPGAYEIPLVAKKMADSKRYDGVICLGA
VIRGATPHFEYVSAEVSKGVAKISLDSNLPVIFGVLTVDTIEQAIERAGTKAGNKGWEAANAAIEMANLLKTI

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=153, Percent_Identity=55.5555555555556, Blast_Score=173, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6324429, Length=147, Percent_Identity=38.0952380952381, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_DESRM (A4J6A4)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113432.1
- ProteinModelPortal:   A4J6A4
- SMR:   A4J6A4
- STRING:   A4J6A4
- GeneID:   4955321
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2090
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   A4J6A4
- BioCyc:   DRED349161:DRED_2090-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16570; Mature: 16439

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKVFEGHLLGQELKFGIVVGRFNEFITNKLLSGALDALKRHGVEDENVEIAYVPGAYEI
CCCCCCCCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
PLVAKKMADSKRYDGVICLGAVIRGATPHFEYVSAEVSKGVAKISLDSNLPVIFGVLTVD
HHHHHHHHCCCCCCCEEEEHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCEEEHHHHHH
TIEQAIERAGTKAGNKGWEAANAAIEMANLLKTI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKVFEGHLLGQELKFGIVVGRFNEFITNKLLSGALDALKRHGVEDENVEIAYVPGAYEI
CCCCCCCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
PLVAKKMADSKRYDGVICLGAVIRGATPHFEYVSAEVSKGVAKISLDSNLPVIFGVLTVD
HHHHHHHHCCCCCCCEEEEHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCEEEHHHHHH
TIEQAIERAGTKAGNKGWEAANAAIEMANLLKTI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA