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The map label for this gene is rlmI [H]

Identifier: 134299906

GI number: 134299906

Start: 2255718

End: 2256974

Strand: Reverse

Name: rlmI [H]

Synonym: Dred_2060

Alternate gene names: 134299906

Gene position: 2256974-2255718 (Counterclockwise)

Preceding gene: 134299907

Following gene: 134299903

Centisome position: 62.55

GC content: 44.71

Gene sequence:

>1257_bases
ATGCTCACTTCCATTTTTATATTAGCGCATAAATCATGTGGTGATACTATTAAGAATTGGGTGAATTGGGGGTTTGTATT
GATGACCAAAGTAGTATTGCATCAGGGAAAACAGAAACGCATTGCTGGGGGGCATCCCTGGGTCTATTGGGGTGAAATAA
AAGAAATTATTGGGGATTTTAATCCCGGTGATATCGTAGAGGTCGTAGATTTTCGTGATCGGTTTGTGGGGCGCGGTTAT
ATCAACCCTGCATCGCAAATTACTGTTCGCATCATGACTAGGAATTCAGAGGAAGAAATTAACGAAGAGTTTTTCCGTAA
GAGAATTCAAGGTGCCTGGGAGTATCGTCAGAAGGTTGTAAGGGAGAGCAACGCCTGTCGGGTTATCTTTGGTGAAGCAG
ATTTTTTACCTGCTTTAATTGTCGATAAGTTTGGTGATTATCTGTCTGTACAAACATTGGCCCTTGGTATAGATGTGCAT
AAGGAAACCATCTTTAAGCTTTTACAGGAAATTGTGCAACCTAAGGGGATGTATGAACGAAACGATGTATCGGTGAGAAA
ACTTGAGGGATTACCTCTGATAACCGGATTTATTGGAGAACCCTTTGACCCCAAGGTCGTGATAAAAGAAAATGGCATTC
AATTTGTTGTGGACTTGGAGGGTGGCCAGAAGACAGGGTATTTCCTCGACCAAAGGGAAAATAGGATGGCTTTGCAGGGG
CTGGTGAAGGGAAGCAGGGTACTGGACTGTTTCTGTCATACTGGTACCTTTTCCATGTATGCTACTAAATATGGAGCTAA
GGAAGTTTTAGGATTAGATATAGCGGCCCCTGCTCTGGAAGTGGCCCGGGTCAATGCACAGTTGAACGGTTACGGTGATC
GATGTACCTTTAAGGAATGCAATAGCTTTGATGAGCTAAGGGCCATGGAACGGGCGGAAGAGAAATTTGATGTGGTTATA
TTGGATCCGCCGGCCTTCACCAAGTCCCGTAAGGCCATAGAAGGGGCTATACGGGGATATAAGGAGATTAATCTGCGAGG
CATGAAACTATTACCCCCAGGTGGTTATTTGATTACCTGTTCCTGTTCCTATCACATGCATGAGGATATGTTCCTGGACG
TGATTTTAGATGCTGCCAGAGATGCCGGAAGGCAGCTAAGGCTGGTGGAACTAAGGCGCCAGGCCAAAGATCATCCAATG
TTACTGGGTTATCCAGAAAGCCACTATCTCAAGTGTGTGGTATTGCAGGTCTGGTAG

Upstream 100 bases:

>100_bases
ACCATTGATGAGGAAAGGGTAATGTACGAAGTTAGGGAGCGAGCAGCACGTTTAGCCGGTAAATAGAATTGTTAGCCCGG
AGGAGGAAGTGGGATACTTT

Downstream 100 bases:

>100_bases
TCAGAGAAATAGGTCCGTGACCCCACAGCTATCTTGACAAATCCGATCTGGCTATGGTGTGGGTATCAAGGGCTTCGAAG
CCAAAGCAGCGGTATATGTA

Product: PUA domain-containing protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLTSIFILAHKSCGDTIKNWVNWGFVLMTKVVLHQGKQKRIAGGHPWVYWGEIKEIIGDFNPGDIVEVVDFRDRFVGRGY
INPASQITVRIMTRNSEEEINEEFFRKRIQGAWEYRQKVVRESNACRVIFGEADFLPALIVDKFGDYLSVQTLALGIDVH
KETIFKLLQEIVQPKGMYERNDVSVRKLEGLPLITGFIGEPFDPKVVIKENGIQFVVDLEGGQKTGYFLDQRENRMALQG
LVKGSRVLDCFCHTGTFSMYATKYGAKEVLGLDIAAPALEVARVNAQLNGYGDRCTFKECNSFDELRAMERAEEKFDVVI
LDPPAFTKSRKAIEGAIRGYKEINLRGMKLLPPGGYLITCSCSYHMHEDMFLDVILDAARDAGRQLRLVELRRQAKDHPM
LLGYPESHYLKCVVLQVW

Sequences:

>Translated_418_residues
MLTSIFILAHKSCGDTIKNWVNWGFVLMTKVVLHQGKQKRIAGGHPWVYWGEIKEIIGDFNPGDIVEVVDFRDRFVGRGY
INPASQITVRIMTRNSEEEINEEFFRKRIQGAWEYRQKVVRESNACRVIFGEADFLPALIVDKFGDYLSVQTLALGIDVH
KETIFKLLQEIVQPKGMYERNDVSVRKLEGLPLITGFIGEPFDPKVVIKENGIQFVVDLEGGQKTGYFLDQRENRMALQG
LVKGSRVLDCFCHTGTFSMYATKYGAKEVLGLDIAAPALEVARVNAQLNGYGDRCTFKECNSFDELRAMERAEEKFDVVI
LDPPAFTKSRKAIEGAIRGYKEINLRGMKLLPPGGYLITCSCSYHMHEDMFLDVILDAARDAGRQLRLVELRRQAKDHPM
LLGYPESHYLKCVVLQVW
>Mature_418_residues
MLTSIFILAHKSCGDTIKNWVNWGFVLMTKVVLHQGKQKRIAGGHPWVYWGEIKEIIGDFNPGDIVEVVDFRDRFVGRGY
INPASQITVRIMTRNSEEEINEEFFRKRIQGAWEYRQKVVRESNACRVIFGEADFLPALIVDKFGDYLSVQTLALGIDVH
KETIFKLLQEIVQPKGMYERNDVSVRKLEGLPLITGFIGEPFDPKVVIKENGIQFVVDLEGGQKTGYFLDQRENRMALQG
LVKGSRVLDCFCHTGTFSMYATKYGAKEVLGLDIAAPALEVARVNAQLNGYGDRCTFKECNSFDELRAMERAEEKFDVVI
LDPPAFTKSRKAIEGAIRGYKEINLRGMKLLPPGGYLITCSCSYHMHEDMFLDVILDAARDAGRQLRLVELRRQAKDHPM
LLGYPESHYLKCVVLQVW

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=395, Percent_Identity=37.7215189873418, Blast_Score=297, Evalue=9e-82,
Organism=Escherichia coli, GI1787181, Length=196, Percent_Identity=29.0816326530612, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 47585; Mature: 47585

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00018 EF_HAND_1 ; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSIFILAHKSCGDTIKNWVNWGFVLMTKVVLHQGKQKRIAGGHPWVYWGEIKEIIGDF
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCC
NPGDIVEVVDFRDRFVGRGYINPASQITVRIMTRNSEEEINEEFFRKRIQGAWEYRQKVV
CCCCEEEHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RESNACRVIFGEADFLPALIVDKFGDYLSVQTLALGIDVHKETIFKLLQEIVQPKGMYER
CCCCCEEEEECCCCCHHHHHHHHCCCHHEEEEEEEEEEHHHHHHHHHHHHHHCCCCCCCC
NDVSVRKLEGLPLITGFIGEPFDPKVVIKENGIQFVVDLEGGQKTGYFLDQRENRMALQG
CCCCEEEECCCCEEECCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHH
LVKGSRVLDCFCHTGTFSMYATKYGAKEVLGLDIAAPALEVARVNAQLNGYGDRCTFKEC
HHCCCCEEEEEECCCCCEEEEECCCCHHHHCCCCCCCHHHHHEECEEECCCCCCCCHHHC
NSFDELRAMERAEEKFDVVILDPPAFTKSRKAIEGAIRGYKEINLRGMKLLPPGGYLITC
CCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCHHEECCCCEEEECCCCEEEEE
SCSYHMHEDMFLDVILDAARDAGRQLRLVELRRQAKDHPMLLGYPESHYLKCVVLQVW
EECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEC
>Mature Secondary Structure
MLTSIFILAHKSCGDTIKNWVNWGFVLMTKVVLHQGKQKRIAGGHPWVYWGEIKEIIGDF
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCC
NPGDIVEVVDFRDRFVGRGYINPASQITVRIMTRNSEEEINEEFFRKRIQGAWEYRQKVV
CCCCEEEHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RESNACRVIFGEADFLPALIVDKFGDYLSVQTLALGIDVHKETIFKLLQEIVQPKGMYER
CCCCCEEEEECCCCCHHHHHHHHCCCHHEEEEEEEEEEHHHHHHHHHHHHHHCCCCCCCC
NDVSVRKLEGLPLITGFIGEPFDPKVVIKENGIQFVVDLEGGQKTGYFLDQRENRMALQG
CCCCEEEECCCCEEECCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHH
LVKGSRVLDCFCHTGTFSMYATKYGAKEVLGLDIAAPALEVARVNAQLNGYGDRCTFKEC
HHCCCCEEEEEECCCCCEEEEECCCCHHHHCCCCCCCHHHHHEECEEECCCCCCCCHHHC
NSFDELRAMERAEEKFDVVILDPPAFTKSRKAIEGAIRGYKEINLRGMKLLPPGGYLITC
CCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCHHEECCCCEEEECCCCEEEEE
SCSYHMHEDMFLDVILDAARDAGRQLRLVELRRQAKDHPMLLGYPESHYLKCVVLQVW
EECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA