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The map label for this gene is nuoB

Identifier: 134299891

GI number: 134299891

Start: 2244497

End: 2245087

Strand: Reverse

Name: nuoB

Synonym: Dred_2045

Alternate gene names: 134299891

Gene position: 2245087-2244497 (Counterclockwise)

Preceding gene: 134299892

Following gene: 134299890

Centisome position: 62.22

GC content: 47.55

Gene sequence:

>591_bases
GTGGAAATAAAGGATCAAGACAAAGCCGCCAACCCGGTAGCTAAAGATGAGATGTATGAGGAAACCGTAAAACGTTTAAA
AAAGCTAGGGGCTGAACCGGCTGTCATCGGGGAGGTTGCTCGAACCGTGTCCGTTGGTCCCTTGGAAAAACTCTTGAATC
TGGCTAGGGCAAATTCACTCTGGCCTATGGGTTTTGGTTTGGCCTGTTGTGCCATTGAAGGCTTAATGGCAGCTAATATG
GCTAGGTTTGATTTAGCCAGATTTGGCTATGAGGTAATGCGGGCATCTCCACGACAAGCAGACGTGATGCTGGTATGCGG
CACGGTGACTAAAAAAGCAGCCCCCTTTGTGGTTCGTCTGTACGAGCAAATGGCAGAGCCAAAATATGTCATTGCCATGG
GTAGCTGTGCTATTTCCGGTGGACCCTTTGTGGATTCATACAGTGTTGTTCCCGGGGTGGATAAATTAATCCCGGTGGAT
GTCTATATTCCTGGTTGTGCCCCCCGGCCCGATGCAGTTATTCATGGGTTTATGAAATTAAAAGAAAAAATTATTAATCC
TGAGGTGGTGAAACGTGGCGATGAACGGTAA

Upstream 100 bases:

>100_bases
GTTTTGTAGATCTTGGTTTGGCAGGACTTATTAAAATGTTTCTTTTCATCTTTATTCTTGTTTTAGGACTTTGGTATGCC
TGGAAGGAAGGTGCACTTGA

Downstream 100 bases:

>100_bases
ACCACTGGCAGAAATTATCCAAGAACTTACAAATAAATACCCTTCATTAGAGGTATCCGAAAAAGGACATTTAACAGTGC
CGGTAGAGTCACTGATGTTA

Product: NADH-quinone oxidoreductase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MEIKDQDKAANPVAKDEMYEETVKRLKKLGAEPAVIGEVARTVSVGPLEKLLNLARANSLWPMGFGLACCAIEGLMAANM
ARFDLARFGYEVMRASPRQADVMLVCGTVTKKAAPFVVRLYEQMAEPKYVIAMGSCAISGGPFVDSYSVVPGVDKLIPVD
VYIPGCAPRPDAVIHGFMKLKEKIINPEVVKRGDER

Sequences:

>Translated_196_residues
MEIKDQDKAANPVAKDEMYEETVKRLKKLGAEPAVIGEVARTVSVGPLEKLLNLARANSLWPMGFGLACCAIEGLMAANM
ARFDLARFGYEVMRASPRQADVMLVCGTVTKKAAPFVVRLYEQMAEPKYVIAMGSCAISGGPFVDSYSVVPGVDKLIPVD
VYIPGCAPRPDAVIHGFMKLKEKIINPEVVKRGDER
>Mature_196_residues
MEIKDQDKAANPVAKDEMYEETVKRLKKLGAEPAVIGEVARTVSVGPLEKLLNLARANSLWPMGFGLACCAIEGLMAANM
ARFDLARFGYEVMRASPRQADVMLVCGTVTKKAAPFVVRLYEQMAEPKYVIAMGSCAISGGPFVDSYSVVPGVDKLIPVD
VYIPGCAPRPDAVIHGFMKLKEKIINPEVVKRGDER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=165, Percent_Identity=48.4848484848485, Blast_Score=164, Evalue=6e-41,
Organism=Escherichia coli, GI1788624, Length=147, Percent_Identity=57.1428571428571, Blast_Score=174, Evalue=4e-45,
Organism=Escherichia coli, GI1788834, Length=112, Percent_Identity=44.6428571428571, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1789074, Length=112, Percent_Identity=44.6428571428571, Blast_Score=95, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=137, Percent_Identity=52.5547445255474, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI18859983, Length=167, Percent_Identity=47.9041916167665, Blast_Score=159, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24642371, Length=167, Percent_Identity=47.9041916167665, Blast_Score=159, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24651058, Length=137, Percent_Identity=52.5547445255474, Blast_Score=157, Evalue=5e-39,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_DESRM (A4J659)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113387.1
- ProteinModelPortal:   A4J659
- SMR:   A4J659
- STRING:   A4J659
- GeneID:   4958621
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2045
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   MAANMAR
- PhylomeDB:   A4J659
- ProtClustDB:   CLSK2537093
- BioCyc:   DRED349161:DRED_2045-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 21346; Mature: 21346

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKDQDKAANPVAKDEMYEETVKRLKKLGAEPAVIGEVARTVSVGPLEKLLNLARANSL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCC
WPMGFGLACCAIEGLMAANMARFDLARFGYEVMRASPRQADVMLVCGTVTKKAAPFVVRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHH
YEQMAEPKYVIAMGSCAISGGPFVDSYSVVPGVDKLIPVDVYIPGCAPRPDAVIHGFMKL
HHHHCCCCEEEEECCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
KEKIINPEVVKRGDER
HHHHCCHHHHHCCCCC
>Mature Secondary Structure
MEIKDQDKAANPVAKDEMYEETVKRLKKLGAEPAVIGEVARTVSVGPLEKLLNLARANSL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCC
WPMGFGLACCAIEGLMAANMARFDLARFGYEVMRASPRQADVMLVCGTVTKKAAPFVVRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHH
YEQMAEPKYVIAMGSCAISGGPFVDSYSVVPGVDKLIPVDVYIPGCAPRPDAVIHGFMKL
HHHHCCCCEEEEECCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
KEKIINPEVVKRGDER
HHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA