Click here to switch to the map view.

The map label for this gene is nuoI [H]

Identifier: 134299887

GI number: 134299887

Start: 2241369

End: 2241866

Strand: Reverse

Name: nuoI [H]

Synonym: Dred_2041

Alternate gene names: 134299887

Gene position: 2241866-2241369 (Counterclockwise)

Preceding gene: 134299888

Following gene: 134299886

Centisome position: 62.13

GC content: 43.57

Gene sequence:

>498_bases
TTGCATGGCAAGGGTCTAATAAAGGGTCTGGGAGTGACGATTAAGCATTTTTTTAAACCAAAGGTAACGGTGCAATACCC
CGAGGTGCGATTGCCAATCCCAGAGAGATTTTTCGGCAGGCCACAGTTTTTTTATGATAAGTGCATTGCCTGTAACCAGT
GTGTGAACGCTTGTCCCAATAATGTTATAAAACTGGAAACAGATACAGTGGATAAAAAGAAAGTGGTGACCAGATACGAT
TTTGACCAGCAGTACTGTATGTTTTGTGGTATGTGTCAAGAGGCTTGTCCGAAGGATGCAATTAAATTTTCGGATGACTT
TGAGTTAACCCAATATAACCGCCCAGATATTAAGATTCAATTTGTTTCACCGGAGCAGGTTGAAAAGAAAAAAGAAGAAC
TTAGAAGGGCGGCGGCGGAAAAGGCTGCGGCTGAAGCAGCTAAAGCTAGGGAGGAAGCGAAGGAAAATCCCGAAGGAACA
GGCAAGGAGGGGATGTAA

Upstream 100 bases:

>100_bases
TGGAATTTGGTTGGAAGGTACTGGTTCCTCTTTCTATTGCGAATATTTTCTTAACTGGTATTGGTAAATATCTGTATCAA
ACACTAGGGTGGTGATAGCA

Downstream 100 bases:

>100_bases
TGGAGGATACCAATGTGATGATAGCCTTTGTCCTGCTGAGCGGGTTGGTCCTGGGTTCTGCCCTGCTGGTGGTAACCTTA
AAAAACTTGGTACACAGTGT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MHGKGLIKGLGVTIKHFFKPKVTVQYPEVRLPIPERFFGRPQFFYDKCIACNQCVNACPNNVIKLETDTVDKKKVVTRYD
FDQQYCMFCGMCQEACPKDAIKFSDDFELTQYNRPDIKIQFVSPEQVEKKKEELRRAAAEKAAAEAAKAREEAKENPEGT
GKEGM

Sequences:

>Translated_165_residues
MHGKGLIKGLGVTIKHFFKPKVTVQYPEVRLPIPERFFGRPQFFYDKCIACNQCVNACPNNVIKLETDTVDKKKVVTRYD
FDQQYCMFCGMCQEACPKDAIKFSDDFELTQYNRPDIKIQFVSPEQVEKKKEELRRAAAEKAAAEAAKAREEAKENPEGT
GKEGM
>Mature_165_residues
MHGKGLIKGLGVTIKHFFKPKVTVQYPEVRLPIPERFFGRPQFFYDKCIACNQCVNACPNNVIKLETDTVDKKKVVTRYD
FDQQYCMFCGMCQEACPKDAIKFSDDFELTQYNRPDIKIQFVSPEQVEKKKEELRRAAAEKAAAEAAKAREEAKENPEGT
GKEGM

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=111, Percent_Identity=38.7387387387387, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1788617, Length=130, Percent_Identity=34.6153846153846, Blast_Score=80, Evalue=5e-17,
Organism=Escherichia coli, GI1789075, Length=94, Percent_Identity=37.2340425531915, Blast_Score=78, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17555194, Length=111, Percent_Identity=39.6396396396396, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17864306, Length=111, Percent_Identity=39.6396396396396, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18866; Mature: 18866

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
4.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGKGLIKGLGVTIKHFFKPKVTVQYPEVRLPIPERFFGRPQFFYDKCIACNQCVNACPN
CCCCCHHHHHHHHHHHHCCCCEEEECCCEECCCCHHHCCCCHHHHHHHHHHHHHHHHCCC
NVIKLETDTVDKKKVVTRYDFDQQYCMFCGMCQEACPKDAIKFSDDFELTQYNRPDIKIQ
CEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCEEEE
FVSPEQVEKKKEELRRAAAEKAAAEAAKAREEAKENPEGTGKEGM
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MHGKGLIKGLGVTIKHFFKPKVTVQYPEVRLPIPERFFGRPQFFYDKCIACNQCVNACPN
CCCCCHHHHHHHHHHHHCCCCEEEECCCEECCCCHHHCCCCHHHHHHHHHHHHHHHHCCC
NVIKLETDTVDKKKVVTRYDFDQQYCMFCGMCQEACPKDAIKFSDDFELTQYNRPDIKIQ
CEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCEEEE
FVSPEQVEKKKEELRRAAAEKAAAEAAKAREEAKENPEGTGKEGM
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA