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The map label for this gene is nusA [H]

Identifier: 134299807

GI number: 134299807

Start: 2150517

End: 2151671

Strand: Reverse

Name: nusA [H]

Synonym: Dred_1960

Alternate gene names: 134299807

Gene position: 2151671-2150517 (Counterclockwise)

Preceding gene: 134299808

Following gene: 134299806

Centisome position: 59.63

GC content: 40.78

Gene sequence:

>1155_bases
GTGAACAGTGAGTTCTTAGAAGCCTTGAGAGATTTAGAAAAAGAGAAGGGTATTGCAGTGGATGTGCTGTTAGAAGCTAT
TGAAGCGGCCTTGTTATCTGCTTATAAACGGAACTTTGGTTCGTTACAAAACGCAAGGGTCCATATTGATCGAGATACAG
GAGATTATAAAGTATATTCCCAGCGTGCTGTTGTGGAGGAAGTTGAAGACGATCGGGTTGAAATTTCCTTAGAAGATGCA
AGAAAAATTGACCCTCGGTTCAACCTGGAAGATGTGGTGGAAGATGAAGTCACTCCTAGAAATTTTGGCCGTATTGCGGC
GCAGACGGCTAAACAAGTTGTTGTTCAGCGAATTCGTGAGGCGGAAAGAAATATCATTTTTGAAGAATTTTCTAACCGTG
AAGGTGATATTCTTACTGGTATTGTGCAACGAATAGAGAATAAAAATGTTTTTATTGAGTTGGGTAAAACAGAGGCTATT
TTAACACCTTCTGAGCAAATACCGAACGAAGATTATAGACACGGCGATCGGTTGAAAACTTACATAGTGGAGGTTCGCAA
AACCACAAAGGGGCCACAGATTTTAGTGTCCAGGACTCACCCAGGTCTGCTAAAGCGGTTGTTTGAACTGGAGGTCCCTG
AACTTCAAGAAGGAGTTGTTGAATTAAAATCCATTGCCAGGGAAGCCGGTTATCGATCTAAAATTGCTGTTTATTCAAAA
GATGAAAATATTGATCCAGTGGGTGCATGTGTGGGCCCAAAAGGGATGCGTGTACAAAATATTGTTACTGAATTGAACGG
TGAAAAGATTGACATTATAAAATGGAACCAGGATCCATCAAAGTATGTAGCCTCTTCTCTCAGCCCAGCTAAAGTGGTGG
CAGTGGAAGTTTGGGAAGATGAGAAAGTAGCCAGAGTTATTGTGCCTGATTACCAACTTTCCTTAGCCATTGGAAAAGAA
GGACAAAATGCACGTCTTGCTGCAAAGCTGACAGGGTGGAAAATTGATATAAAAAGTGAGTCCCAGATGACAGATATTTA
TCCAGAATATGATGCTGAGGAAAACGATGGATTTTCCACAGATGATAATTATCTAGAAACCTTTGAGGACGAATACAAAC
ATGTTGATTCGTCCGAAGAGAAAAATGGTGAGTAA

Upstream 100 bases:

>100_bases
CACCTGGAGATTAAAGGGAAACAGGTTGTATTACCCCATCAACAGGTTGCCACCGCACGTTTGGCAGTAGAAATTTGATA
TTATTGGGGGGAGCAAAAAA

Downstream 100 bases:

>100_bases
GGAGGTTGTGCAGTGCCCAAGGTCAAAAAGGTTCCGCTAAGGATGTGTATTGGCTGTCAGGAAATGAAGCCTAAGCGAGA
ATTGACAAGGGTGGTGCGGA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNSEFLEALRDLEKEKGIAVDVLLEAIEAALLSAYKRNFGSLQNARVHIDRDTGDYKVYSQRAVVEEVEDDRVEISLEDA
RKIDPRFNLEDVVEDEVTPRNFGRIAAQTAKQVVVQRIREAERNIIFEEFSNREGDILTGIVQRIENKNVFIELGKTEAI
LTPSEQIPNEDYRHGDRLKTYIVEVRKTTKGPQILVSRTHPGLLKRLFELEVPELQEGVVELKSIAREAGYRSKIAVYSK
DENIDPVGACVGPKGMRVQNIVTELNGEKIDIIKWNQDPSKYVASSLSPAKVVAVEVWEDEKVARVIVPDYQLSLAIGKE
GQNARLAAKLTGWKIDIKSESQMTDIYPEYDAEENDGFSTDDNYLETFEDEYKHVDSSEEKNGE

Sequences:

>Translated_384_residues
MNSEFLEALRDLEKEKGIAVDVLLEAIEAALLSAYKRNFGSLQNARVHIDRDTGDYKVYSQRAVVEEVEDDRVEISLEDA
RKIDPRFNLEDVVEDEVTPRNFGRIAAQTAKQVVVQRIREAERNIIFEEFSNREGDILTGIVQRIENKNVFIELGKTEAI
LTPSEQIPNEDYRHGDRLKTYIVEVRKTTKGPQILVSRTHPGLLKRLFELEVPELQEGVVELKSIAREAGYRSKIAVYSK
DENIDPVGACVGPKGMRVQNIVTELNGEKIDIIKWNQDPSKYVASSLSPAKVVAVEVWEDEKVARVIVPDYQLSLAIGKE
GQNARLAAKLTGWKIDIKSESQMTDIYPEYDAEENDGFSTDDNYLETFEDEYKHVDSSEEKNGE
>Mature_384_residues
MNSEFLEALRDLEKEKGIAVDVLLEAIEAALLSAYKRNFGSLQNARVHIDRDTGDYKVYSQRAVVEEVEDDRVEISLEDA
RKIDPRFNLEDVVEDEVTPRNFGRIAAQTAKQVVVQRIREAERNIIFEEFSNREGDILTGIVQRIENKNVFIELGKTEAI
LTPSEQIPNEDYRHGDRLKTYIVEVRKTTKGPQILVSRTHPGLLKRLFELEVPELQEGVVELKSIAREAGYRSKIAVYSK
DENIDPVGACVGPKGMRVQNIVTELNGEKIDIIKWNQDPSKYVASSLSPAKVVAVEVWEDEKVARVIVPDYQLSLAIGKE
GQNARLAAKLTGWKIDIKSESQMTDIYPEYDAEENDGFSTDDNYLETFEDEYKHVDSSEEKNGE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=371, Percent_Identity=40.4312668463612, Blast_Score=298, Evalue=5e-82,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 43592; Mature: 43592

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSEFLEALRDLEKEKGIAVDVLLEAIEAALLSAYKRNFGSLQNARVHIDRDTGDYKVYS
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHH
QRAVVEEVEDDRVEISLEDARKIDPRFNLEDVVEDEVTPRNFGRIAAQTAKQVVVQRIRE
HHHHHHHHCCCEEEEEEHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AERNIIFEEFSNREGDILTGIVQRIENKNVFIELGKTEAILTPSEQIPNEDYRHGDRLKT
HHHHEEHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCEEEECCHHHCCCCHHCCCHHHHH
YIVEVRKTTKGPQILVSRTHPGLLKRLFELEVPELQEGVVELKSIAREAGYRSKIAVYSK
HHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEC
DENIDPVGACVGPKGMRVQNIVTELNGEKIDIIKWNQDPSKYVASSLSPAKVVAVEVWED
CCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCCEEEEEEECCC
EKVARVIVPDYQLSLAIGKEGQNARLAAKLTGWKIDIKSESQMTDIYPEYDAEENDGFST
CCEEEEEECCEEEEEEECCCCCCCEEEEEEECEEEEECCCCCCCCCCCCCCCCCCCCCCC
DDNYLETFEDEYKHVDSSEEKNGE
CCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNSEFLEALRDLEKEKGIAVDVLLEAIEAALLSAYKRNFGSLQNARVHIDRDTGDYKVYS
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHH
QRAVVEEVEDDRVEISLEDARKIDPRFNLEDVVEDEVTPRNFGRIAAQTAKQVVVQRIRE
HHHHHHHHCCCEEEEEEHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AERNIIFEEFSNREGDILTGIVQRIENKNVFIELGKTEAILTPSEQIPNEDYRHGDRLKT
HHHHEEHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCEEEECCHHHCCCCHHCCCHHHHH
YIVEVRKTTKGPQILVSRTHPGLLKRLFELEVPELQEGVVELKSIAREAGYRSKIAVYSK
HHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEC
DENIDPVGACVGPKGMRVQNIVTELNGEKIDIIKWNQDPSKYVASSLSPAKVVAVEVWED
CCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCCEEEEEEECCC
EKVARVIVPDYQLSLAIGKEGQNARLAAKLTGWKIDIKSESQMTDIYPEYDAEENDGFST
CCEEEEEECCEEEEEEECCCCCCCEEEEEEECEEEEECCCCCCCCCCCCCCCCCCCCCCC
DDNYLETFEDEYKHVDSSEEKNGE
CCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]