Click here to switch to the map view.

The map label for this gene is rimO

Identifier: 134299776

GI number: 134299776

Start: 2115867

End: 2117201

Strand: Reverse

Name: rimO

Synonym: Dred_1926

Alternate gene names: 134299776

Gene position: 2117201-2115867 (Counterclockwise)

Preceding gene: 134299777

Following gene: 134299775

Centisome position: 58.68

GC content: 40.37

Gene sequence:

>1335_bases
ATGCCCTCTATCGGATTATTAAGCTTGGGATGCCCAAAAAACCTGGTGGATTCAGAGGTTATGCTAGGTCTACTCCGGGA
AAATAATTTTACAATAACCAATAACGAAGCAAATGCCGATGCTTTAATTGTTAACACATGCGGCTTTATTGAATCCGCCA
AGGAAGAATCCATACGGCATATCTTTGAATTGGCACAATATAAGGAGAGGGGCAAATGTAAGGCGCTTATTGTTACAGGC
TGTTTAGCACAAAGATATTCAAAGGAACTACTGGAAGAAATTCCAGAAATTGATGTTATCCTTGGTCCAGGACATGTCAG
TAATATTGTAGAAGTAGTTAATCACGCTTTGGAAGGCAAGGATAGACACACCCGAGTCGAGGACCTTTTATATATCTATG
ATGAGCATTCGCCCCGTTTGTTAAGTACACCCTCTTATACTGCCTACGTAAAAATTGCAGAGGGTTGTGATAACCGGTGC
GCTTACTGCGCTATACCGGATATCCGAGGTAAATTCCGTAGCAGGCCCATTGAATCCATTGAAGCAGAAGTTAAGGATTT
GGTGGAAAAGGGCGTGAGAGAGATTATTTTAATTGCCCAAGACACCACACGTTACGGGTTGGATTTATACGGTGAATATC
GACTGGACCAGTTATTAGAAAGATTGGGACCCATCCAGGGTCTTCAATGGATTCGATTACTATATTGTTACCCTAATCGT
TTTACGGATCAATTAATTAAGGCTATGGCGGAAAACCCCAATATATGTAAATACATTGATTTACCAATGCAGCATGCAGC
AAATGATATTTTGAGGGCTATGAAACGCCCTGTTACGAAACAACAAGCACGGATACTTATTCAAAAGTTACGAACTGAAA
TACCAGGTATTGTTATTCGGACATCTTTCATTGTTGGTTTCCCAGGGGAAACTGAGGAACATTTTAAAGAACTACTAGAT
TTTATGGAAGAAGTAAAATTTGATAGGGCTGGTGTATTTACATATTCTCAGGAGGAAGGAACCCCGGCAGCAGAAATGCC
TAATCAAATTCATGGCAGAATAAAACAAGAACGTTATCATAGGGCGATGAAATTGCAAAGGGAGATATCCCTTTCGCAAA
ATCAGAAAAGAATTGGCCAAGAAATAGAAGTTGTAGTTGAAGAGGTAACGGACCAAAGCAAAGGGGTTTATACCGGTCGT
TCTTCCTATGATGCGCCGGAGATTGATGGTACCGTAGAATTTACCAGTTCAAGACCATTACGGATTGGTGATTTTGTTAA
AGTAAAGATTAATAGGGCATTAGAGTATGATTTGATGGGAGAGTTGGCCCAATGA

Upstream 100 bases:

>100_bases
GAGTTTACAAATTATAGAACCATTTAGTTCTCTTTGACAGGTTAGTAGCACTGATTTATACTTATTACGAAAAATTCTTC
TAACAACGAAAGGAAATGTT

Downstream 100 bases:

>100_bases
ATTTACCGAACCGGTTGACTCTGGCAAGAATGTTTTTAATTCCTGTTTTTTTGGCAGTGGTTACTTTAAGAATTAAATAT
GGCGATATAATAGCTGCTGG

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MPSIGLLSLGCPKNLVDSEVMLGLLRENNFTITNNEANADALIVNTCGFIESAKEESIRHIFELAQYKERGKCKALIVTG
CLAQRYSKELLEEIPEIDVILGPGHVSNIVEVVNHALEGKDRHTRVEDLLYIYDEHSPRLLSTPSYTAYVKIAEGCDNRC
AYCAIPDIRGKFRSRPIESIEAEVKDLVEKGVREIILIAQDTTRYGLDLYGEYRLDQLLERLGPIQGLQWIRLLYCYPNR
FTDQLIKAMAENPNICKYIDLPMQHAANDILRAMKRPVTKQQARILIQKLRTEIPGIVIRTSFIVGFPGETEEHFKELLD
FMEEVKFDRAGVFTYSQEEGTPAAEMPNQIHGRIKQERYHRAMKLQREISLSQNQKRIGQEIEVVVEEVTDQSKGVYTGR
SSYDAPEIDGTVEFTSSRPLRIGDFVKVKINRALEYDLMGELAQ

Sequences:

>Translated_444_residues
MPSIGLLSLGCPKNLVDSEVMLGLLRENNFTITNNEANADALIVNTCGFIESAKEESIRHIFELAQYKERGKCKALIVTG
CLAQRYSKELLEEIPEIDVILGPGHVSNIVEVVNHALEGKDRHTRVEDLLYIYDEHSPRLLSTPSYTAYVKIAEGCDNRC
AYCAIPDIRGKFRSRPIESIEAEVKDLVEKGVREIILIAQDTTRYGLDLYGEYRLDQLLERLGPIQGLQWIRLLYCYPNR
FTDQLIKAMAENPNICKYIDLPMQHAANDILRAMKRPVTKQQARILIQKLRTEIPGIVIRTSFIVGFPGETEEHFKELLD
FMEEVKFDRAGVFTYSQEEGTPAAEMPNQIHGRIKQERYHRAMKLQREISLSQNQKRIGQEIEVVVEEVTDQSKGVYTGR
SSYDAPEIDGTVEFTSSRPLRIGDFVKVKINRALEYDLMGELAQ
>Mature_443_residues
PSIGLLSLGCPKNLVDSEVMLGLLRENNFTITNNEANADALIVNTCGFIESAKEESIRHIFELAQYKERGKCKALIVTGC
LAQRYSKELLEEIPEIDVILGPGHVSNIVEVVNHALEGKDRHTRVEDLLYIYDEHSPRLLSTPSYTAYVKIAEGCDNRCA
YCAIPDIRGKFRSRPIESIEAEVKDLVEKGVREIILIAQDTTRYGLDLYGEYRLDQLLERLGPIQGLQWIRLLYCYPNRF
TDQLIKAMAENPNICKYIDLPMQHAANDILRAMKRPVTKQQARILIQKLRTEIPGIVIRTSFIVGFPGETEEHFKELLDF
MEEVKFDRAGVFTYSQEEGTPAAEMPNQIHGRIKQERYHRAMKLQREISLSQNQKRIGQEIEVVVEEVTDQSKGVYTGRS
SYDAPEIDGTVEFTSSRPLRIGDFVKVKINRALEYDLMGELAQ

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=473, Percent_Identity=28.5412262156448, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI28872782, Length=473, Percent_Identity=28.5412262156448, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI93277076, Length=456, Percent_Identity=26.9736842105263, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=43.0155210643016, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=29.4117647058824, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=27.4468085106383, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71996771, Length=376, Percent_Identity=25.531914893617, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356207, Length=471, Percent_Identity=28.2377919320594, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI19922432, Length=450, Percent_Identity=26.8888888888889, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_DESRM (A4J5U4)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113272.1
- ProteinModelPortal:   A4J5U4
- SMR:   A4J5U4
- STRING:   A4J5U4
- GeneID:   4957531
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_1926
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   LYLYPDE
- PhylomeDB:   A4J5U4
- BioCyc:   DRED349161:DRED_1926-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50599; Mature: 50468

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIGLLSLGCPKNLVDSEVMLGLLRENNFTITNNEANADALIVNTCGFIESAKEESIRH
CCCCCEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHH
IFELAQYKERGKCKALIVTGCLAQRYSKELLEEIPEIDVILGPGHVSNIVEVVNHALEGK
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCC
DRHTRVEDLLYIYDEHSPRLLSTPSYTAYVKIAEGCDNRCAYCAIPDIRGKFRSRPIESI
CHHHHHHHHEEEEECCCCCEECCCCCEEEEEEECCCCCCCEEEECCHHHHHHHCCCHHHH
EAEVKDLVEKGVREIILIAQDTTRYGLDLYGEYRLDQLLERLGPIQGLQWIRLLYCYPNR
HHHHHHHHHHHHHHEEEEEECCCCCCCHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
FTDQLIKAMAENPNICKYIDLPMQHAANDILRAMKRPVTKQQARILIQKLRTEIPGIVIR
HHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEE
TSFIVGFPGETEEHFKELLDFMEEVKFDRAGVFTYSQEEGTPAAEMPNQIHGRIKQERYH
EEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHH
RAMKLQREISLSQNQKRIGQEIEVVVEEVTDQSKGVYTGRSSYDAPEIDGTVEFTSSRPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEECCCCCE
RIGDFVKVKINRALEYDLMGELAQ
EECCEEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure 
PSIGLLSLGCPKNLVDSEVMLGLLRENNFTITNNEANADALIVNTCGFIESAKEESIRH
CCCCEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHH
IFELAQYKERGKCKALIVTGCLAQRYSKELLEEIPEIDVILGPGHVSNIVEVVNHALEGK
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCC
DRHTRVEDLLYIYDEHSPRLLSTPSYTAYVKIAEGCDNRCAYCAIPDIRGKFRSRPIESI
CHHHHHHHHEEEEECCCCCEECCCCCEEEEEEECCCCCCCEEEECCHHHHHHHCCCHHHH
EAEVKDLVEKGVREIILIAQDTTRYGLDLYGEYRLDQLLERLGPIQGLQWIRLLYCYPNR
HHHHHHHHHHHHHHEEEEEECCCCCCCHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
FTDQLIKAMAENPNICKYIDLPMQHAANDILRAMKRPVTKQQARILIQKLRTEIPGIVIR
HHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEE
TSFIVGFPGETEEHFKELLDFMEEVKFDRAGVFTYSQEEGTPAAEMPNQIHGRIKQERYH
EEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHH
RAMKLQREISLSQNQKRIGQEIEVVVEEVTDQSKGVYTGRSSYDAPEIDGTVEFTSSRPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEECCCCCE
RIGDFVKVKINRALEYDLMGELAQ
EECCEEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA