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The map label for this gene is miaB

Identifier: 134299740

GI number: 134299740

Start: 2076175

End: 2077545

Strand: Reverse

Name: miaB

Synonym: Dred_1889

Alternate gene names: 134299740

Gene position: 2077545-2076175 (Counterclockwise)

Preceding gene: 134299741

Following gene: 134299739

Centisome position: 57.58

GC content: 43.54

Gene sequence:

>1371_bases
ATGGCTGAAAAGCACCAAGATAAAATGACAAATGCAATGAATAATTCTAAACTGTACCTTATTCAATCCTTTGGTTGCCA
AATGAACGAAAGGGACGCAGAATCCCTAGCTGGTATGCTGGAGGATTTGGGTTATTGTCCTACGAGTGCTCAAGAAGAAG
CAGATATTATTTTGTTAAATACCTGTTGTGTCCGGGAGACAGCTGAGAGCAAAGTCTTTGGTCTACTGGGACGTCTGAGA
AAACTGAAGGTGGCGAAACCAGATCTGATTTTAGGTGTTTGTGGTTGTATGTCCCAACAGGAAGATGCGGCCAAGCGCAT
TAGACGTAGTTTTCCCTTCGTTGATTTGATTTTTGGCACCCATAATATTCATGAATTGCCTCGCATGATTCATCAAGTAC
AGGAAAACCATGAGGCAGTGCTGGAAGTTTGGGCCACTGAAAAAGGAATCACGGAAAGCATTCCAGTAAAACGTAAGGAT
AAACTAAAGGCTTGGGTAACCATCATGTACGGGTGTAATAACTTCTGCACCTACTGCATTGTGCCCTATGTCAGAGGCAG
AGAGCGCAGTCGCCAACCCGAAGATATTATTGATGAGATAAAGGAATTAGTTCAGGAAGGCTATAAAGAAGTGACCTTGC
TTGGGCAAAATGTAAATTCCTACGGCAAAGATTTTAAGAATAATTACCGTTTTGCAGATCTTCTGATGGCTATTGATGAT
ATTACGGGACTGGAGCGTGTAAGGTTTATGACCTCGCACCCCAGAGACTTTGATCAGAGGCTTATAGAAGTGGTGGCATC
CGCAAAAAAGGTTTGTGAACATTACCATCTTCCTGCACAGGCTGGTAGTAATCGAGTATTAAAAATGATGAACCGTGGTT
ACACCAGGGAACATTATTTGGAGTTAATTAGAAAGATCAAAGAGAGGGTTCCCAATGCCAGTATTACTGCAGATCTAATG
GTTGGGTTCCCGGGAGAAACTGAGGAAGATTTTCAGGAGACCCTTGATTTAGTGAAGCAGGTTCGGTATGATAGTGCCTT
CACCTTTGTATACAATATTCGTTCCGGAACTCCGGCGGCCAAGCTGGAACAAGTTTCGGAGGAGGTCAAGAGTGAACGTA
TACAAAGGTTAATTGAACTACAAAACCTTATTTCTCTTGAAAATAACCAACGGGAAGAGGGCAGGGTCTTAGAAGTCCTG
GTAGAGGGAGAGACCAAAACCAACCCGGATCTGTTGGCGGGACGTACCAGAACAAACAAACTTGTTGTTTTTCAAGGGTC
TGGTCACTTGCCAGGGCAACTGGTGCAGATTAGAATCACAAAGGGACGTCCAAATTTGTTAGAGGGAGAAGTGGTACCCA
ATGGTACCTAG

Upstream 100 bases:

>100_bases
AAAACTAAAAGTAATAGTTTGCAATCCCTTAGTAAATATGGGAAAATGTTATTGTTCGCCATGAACTTTCCATTTTAGCT
TTTTGGAGGAAAACAGATAG

Downstream 100 bases:

>100_bases
CATAAATTCTCTTATATCCTTGGGGGAGGTGAAAAATATGAGTAATGTGGTTTTAGAGAGAGCTTTAGAATTGGGTAAAC
TCATTGCCCAGTCTGAGGAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAEKHQDKMTNAMNNSKLYLIQSFGCQMNERDAESLAGMLEDLGYCPTSAQEEADIILLNTCCVRETAESKVFGLLGRLR
KLKVAKPDLILGVCGCMSQQEDAAKRIRRSFPFVDLIFGTHNIHELPRMIHQVQENHEAVLEVWATEKGITESIPVKRKD
KLKAWVTIMYGCNNFCTYCIVPYVRGRERSRQPEDIIDEIKELVQEGYKEVTLLGQNVNSYGKDFKNNYRFADLLMAIDD
ITGLERVRFMTSHPRDFDQRLIEVVASAKKVCEHYHLPAQAGSNRVLKMMNRGYTREHYLELIRKIKERVPNASITADLM
VGFPGETEEDFQETLDLVKQVRYDSAFTFVYNIRSGTPAAKLEQVSEEVKSERIQRLIELQNLISLENNQREEGRVLEVL
VEGETKTNPDLLAGRTRTNKLVVFQGSGHLPGQLVQIRITKGRPNLLEGEVVPNGT

Sequences:

>Translated_456_residues
MAEKHQDKMTNAMNNSKLYLIQSFGCQMNERDAESLAGMLEDLGYCPTSAQEEADIILLNTCCVRETAESKVFGLLGRLR
KLKVAKPDLILGVCGCMSQQEDAAKRIRRSFPFVDLIFGTHNIHELPRMIHQVQENHEAVLEVWATEKGITESIPVKRKD
KLKAWVTIMYGCNNFCTYCIVPYVRGRERSRQPEDIIDEIKELVQEGYKEVTLLGQNVNSYGKDFKNNYRFADLLMAIDD
ITGLERVRFMTSHPRDFDQRLIEVVASAKKVCEHYHLPAQAGSNRVLKMMNRGYTREHYLELIRKIKERVPNASITADLM
VGFPGETEEDFQETLDLVKQVRYDSAFTFVYNIRSGTPAAKLEQVSEEVKSERIQRLIELQNLISLENNQREEGRVLEVL
VEGETKTNPDLLAGRTRTNKLVVFQGSGHLPGQLVQIRITKGRPNLLEGEVVPNGT
>Mature_455_residues
AEKHQDKMTNAMNNSKLYLIQSFGCQMNERDAESLAGMLEDLGYCPTSAQEEADIILLNTCCVRETAESKVFGLLGRLRK
LKVAKPDLILGVCGCMSQQEDAAKRIRRSFPFVDLIFGTHNIHELPRMIHQVQENHEAVLEVWATEKGITESIPVKRKDK
LKAWVTIMYGCNNFCTYCIVPYVRGRERSRQPEDIIDEIKELVQEGYKEVTLLGQNVNSYGKDFKNNYRFADLLMAIDDI
TGLERVRFMTSHPRDFDQRLIEVVASAKKVCEHYHLPAQAGSNRVLKMMNRGYTREHYLELIRKIKERVPNASITADLMV
GFPGETEEDFQETLDLVKQVRYDSAFTFVYNIRSGTPAAKLEQVSEEVKSERIQRLIELQNLISLENNQREEGRVLEVLV
EGETKTNPDLLAGRTRTNKLVVFQGSGHLPGQLVQIRITKGRPNLLEGEVVPNGT

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=36.3070539419087, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=36.4016736401674, Blast_Score=300, Evalue=3e-81,
Organism=Homo sapiens, GI93277076, Length=448, Percent_Identity=27.6785714285714, Blast_Score=172, Evalue=6e-43,
Organism=Escherichia coli, GI1786882, Length=436, Percent_Identity=42.2018348623853, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1787057, Length=407, Percent_Identity=30.2211302211302, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=473, Percent_Identity=35.0951374207188, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI71996771, Length=294, Percent_Identity=29.9319727891156, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356207, Length=497, Percent_Identity=34.0040241448692, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI19922432, Length=450, Percent_Identity=28.8888888888889, Blast_Score=171, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_DESRM (A4J5Q8)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113236.1
- ProteinModelPortal:   A4J5Q8
- SMR:   A4J5Q8
- STRING:   A4J5Q8
- GeneID:   4955393
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_1889
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- PhylomeDB:   A4J5Q8
- ProtClustDB:   PRK14328
- BioCyc:   DRED349161:DRED_1889-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51997; Mature: 51866

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKHQDKMTNAMNNSKLYLIQSFGCQMNERDAESLAGMLEDLGYCPTSAQEEADIILLN
CCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
TCCVRETAESKVFGLLGRLRKLKVAKPDLILGVCGCMSQQEDAAKRIRRSFPFVDLIFGT
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCC
HNIHELPRMIHQVQENHEAVLEVWATEKGITESIPVKRKDKLKAWVTIMYGCNNFCTYCI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
VPYVRGRERSRQPEDIIDEIKELVQEGYKEVTLLGQNVNSYGKDFKNNYRFADLLMAIDD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEHCCCCHHHHHHHHHCCCHHHHHHHHHHH
ITGLERVRFMTSHPRDFDQRLIEVVASAKKVCEHYHLPAQAGSNRVLKMMNRGYTREHYL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHH
ELIRKIKERVPNASITADLMVGFPGETEEDFQETLDLVKQVRYDSAFTFVYNIRSGTPAA
HHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHH
KLEQVSEEVKSERIQRLIELQNLISLENNQREEGRVLEVLVEGETKTNPDLLAGRTRTNK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHEECCCCCCE
LVVFQGSGHLPGQLVQIRITKGRPNLLEGEVVPNGT
EEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEKHQDKMTNAMNNSKLYLIQSFGCQMNERDAESLAGMLEDLGYCPTSAQEEADIILLN
CCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
TCCVRETAESKVFGLLGRLRKLKVAKPDLILGVCGCMSQQEDAAKRIRRSFPFVDLIFGT
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCC
HNIHELPRMIHQVQENHEAVLEVWATEKGITESIPVKRKDKLKAWVTIMYGCNNFCTYCI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
VPYVRGRERSRQPEDIIDEIKELVQEGYKEVTLLGQNVNSYGKDFKNNYRFADLLMAIDD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEHCCCCHHHHHHHHHCCCHHHHHHHHHHH
ITGLERVRFMTSHPRDFDQRLIEVVASAKKVCEHYHLPAQAGSNRVLKMMNRGYTREHYL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHH
ELIRKIKERVPNASITADLMVGFPGETEEDFQETLDLVKQVRYDSAFTFVYNIRSGTPAA
HHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHH
KLEQVSEEVKSERIQRLIELQNLISLENNQREEGRVLEVLVEGETKTNPDLLAGRTRTNK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHEECCCCCCE
LVVFQGSGHLPGQLVQIRITKGRPNLLEGEVVPNGT
EEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA